Malvern NANOSIGHT NS300 Operating Manual
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Malvern NANOSIGHT NS300 Operating Manual

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NANOSIGHT NS300

OPERATING MANUAL

MAN0516-05-EN-00 September 2014

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  • Page 1: Operating Manual

    NANOSIGHT NS300 OPERATING MANUAL MAN0516-05-EN-00 September 2014...
  • Page 2 Worcestershire WR14 1XZ United Kingdom. Tel + [44] (0)1684-892456 Fax + [44] (0)1684-892789 www.malvern.com NanoSight, Malvern and the 'hills' logo are registered trademarks in the UK and/or other countries, and are owned by Malvern Instruments Ltd. NanoSight NS300 Operating Manual (MAN0516-05-EN-00)

  • Page 3: Table Of Contents

      1.3.7 R ..........................8 ETURNING QUIPMENT   1.3.8 W ............................. 8 ARRANTY   2. NANOSIGHT NS300 HARDWARE ........................ 9   2.1 NS300 TECHNICAL SPECIFICATIONS ......................9   2.1.1 NS300 I ........................9 NSTRUMENT OUSING   2.1.2 NS300 L ..........................9...
  • Page 4   3. NANOSIGHT NS300 CONTROL SOFTWARE ..................... 21   3.1 NTA S ..........................21 OFTWARE TARTUP   3.2 C ............................22 AMERA ETUP   3.3 A NS300 ....................22 DJUSTING THE TO OBTAIN A VIDEO   3.4 T ) .................. 23...

  • Page 5: Introduction

    Other solvents may not be compatible with wetted surfaces and should not be used without confirming suitability. If in doubt about the choice of solvent and its compatibility with any part of the device, please contact Malvern (helpdesk@Malvern.com) for further information.

  • Page 6: Electrical Safety

    • The instrument can be used with all non-flammable water-based solvents of neutral pH. Other solvents may not be compatible and must not be used without first contacting Malvern to confirm suitability. • When using bio or chemically hazardous sample material, it is the responsibility of the operator to determine the requisite protection for each application.

  • Page 7: Servicing

    Between uses of the NanoSight NS300, or for longer term storage, the unit must be cleaned and dried as described in this manual.

  • Page 8: Installation And Relocation

    Malvern before returning any equipment, and should be clearly displayed on the return shipment and included on all subsequent correspondence. If the original shipping case is not available, shipping cases will be supplied by Malvern as required to ensure safe return of the system 1.3.8 Warranty...

  • Page 9: Nanosight Ns300 Hardware

    2. NanoSight NS300 Hardware 2.1 NS300 Technical Specifications 2.1.1 NS300 Instrument Housing Size: 400 mm x 400 mm x 250 mm Weight: ~12 kg Operating temperature: 10 °C to 40 °C Humidity: Up to 80% rH at 31 °C then decreasing linearly to 50% at 40 °C...

  • Page 10: Ns300 Operation

    Between uses of the instrument, the sprung fastening screws holding the top-plate down should be fully loosened, so that the optical flat is not held under any pressure. Failure to loosen the top-plate screws between uses can result in damage to the optical flat. NanoSight NS300 Operating Manual (MAN0516-05-EN-00)

  • Page 11: Laser Module Configuration

    The sample chamber configuration and the instructions for cleaning will vary according to which design of top-plate is being used. Please refer to Section 2.2.3.1 and 2.2.4.1 for the flow-cell top-plate and Section 2.2.3.2 and 2.2.4.2 for the O- ring top-plate, as appropriate. Flow-cell top plate O-ring top-plate NanoSight NS300 Operating Manual (MAN0516-05-EN-00)

  • Page 12: Flow-Cell Top-Plate

    NS300. NS300. For instructions on how to clean and assemble the flow-cell top plate see Section 2.2.4.1 NanoSight NS300 Operating Manual (MAN0516-05-EN-00)

  • Page 13: O-Ring Top-Plate

    Section 2.2.5). Once the sample has been loaded, the laser module should be placed into the NS300, keeping the syringe connected For instructions on how to assemble and clean the O-ring top plate see Section 2.2.4.2 NanoSight NS300 Operating Manual (MAN0516-05-EN-00)
  • Page 14 The fluidic tubing connection is intended for use with the syringe pump accessory only. Manually loading sample through a syringe with the waste tubing connected can exert high pressures on the chamber seal. This can result in sample leakage or damage to the top-plate. NanoSight NS300 Operating Manual (MAN0516-05-EN-00)

  • Page 15: Assembling And Cleaning The Laser Module

    It is important to take care not to abrade or scratch the optical flat surface, or introduce particulates or contaminants onto the surface. Treat all optical surfaces with the same care as would be employed with equivalent surfaces on your microscope. Should the optical flat in the laser module become damaged, it can be replaced by Malvern (pricing on request).
  • Page 16 Important: Avoid touching or rubbing the seal when drying the underside. Keep the compressed air nozzle at least 5cm away from the seal, to avoid high pressure damaging it Important: If the gasket component repeatedly leaks after a manual clean, it should be replaced (Part No. NTA4027). NanoSight NS300 Operating Manual (MAN0516-05-EN-00)

  • Page 17: O-Ring Top-Plate

    Unscrew the sprung fastening screws securing the top plate and lift the top plate gently off the laser module. Rinse the top-plate with water, or water then ethanol if needed, including the O-ring seal and inside the fluidic ports. NanoSight NS300 Operating Manual (MAN0516-05-EN-00)

  • Page 18: Loading A Sample

    These can sometimes be removed by carefully moving fresh sample through the chamber, allowing air bubbles to disperse. If samples repeatedly show evidence of bubble formation, it is advisable to de-gas the sample before analysis. NanoSight NS300 Operating Manual (MAN0516-05-EN-00)

  • Page 19: Using The Flow-Cell Top-Plate

    Once liquid is seen emerging in the waste tubing, place the syringe into the syringe pump holder as described in the NanoSight Syringe Pump Operating Manual Operate the syringe pump as described in the manual. NanoSight NS300 Operating Manual (MAN0516-05-EN-00)

  • Page 20: Mounting The Laser Module

    Rinse the chamber by slowly (i.e. 1ml syringe in 5-10 seconds) loading clean water or a suitable cleaning solution (please contact Malvern for advice on customised cleaning procedures) Confirm the cleanliness of the chamber by checking for any particles present in the NTA software image, before loading the next sample as described in Section 2.2.5.1.

  • Page 21: Nanosight Ns300 Control Software

    Run the NTA Software. NanoSi The NanoSight NTA software comes pre-installed on Malvern-supplied computers and can be run either from the desktop icon or via the start menu (under All Programs > NanoSight). On opening the NTA Program, the following screen appears:...

  • Page 22: Camera Setup

    3.3 Adjusting the NS300 to obtain a video The NS300 imaging position is set-up and calibrated by Malvern. The system is designed to have good beam relocation once set-up, although small adjustments may be necessary to optimise the image. If the beam appears not to be central in the field of view on screen, i.e.

  • Page 23: Thresholding Pixels (High-Sensitivity Systems Only)

    Filter 2 etc. to move the selected filter into the field of view. Adjust the grey histogram (see Section 3.4) so that the image intensity scale is restricted as much as possible. Adjust the focus as necessary and begin video capture. NanoSight NS300 Operating Manual (MAN0516-05-EN-00)

  • Page 24: Capture Videos

    Before using the temperature controller it is imperative that all the warnings mentioned above are read and understood. NanoSight NS300 Operating Manual (MAN0516-05-EN-00)

  • Page 25: Initialising

    A new temperature can be programmed to change the temperature (by repeating steps 2 and 3). When finished with the system press the ‘off’ button in NTA to turn off the temperature controller. The temperature readout will continue. NanoSight NS300 Operating Manual (MAN0516-05-EN-00)
  • Page 26 When planning a measurement, the temperature at which the measurement should be taken can be set by clicking in the ‘Target temperature’ box under the SOP tab. Type in the temperature required Tick the ‘Use Temperature Control’ button. NanoSight NS300 Operating Manual (MAN0516-05-EN-00)

  • Page 27: Spare Parts List

    Replacement O-ring top-plate luer ports angled (Pack of 10) NTA4027 Flow-cell gasket component NTA4049 Flow-cell fixing bolt and nut (2) & 2mm Allen key NTA4109 Top-plate sprung fastening screws (Pack of 4) BOT0048 NS300 250ml Waste Bottle NanoSight NS300 Operating Manual (MAN0516-05-EN-00)

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