Neutralization Mode Operation - Thermo Scientific Dionex ERS 500 Product Manual

3 – Operation
C. Installation of the Dionex CTC-1 Trap Column
The Dionex CTC-1 Trap Column (Item # 040192) is used to trap cations that may be in the
carrier solution that could result in high blanks. The Dionex CTC-1 should be regenerated
prior to installation.
D. Regeneration of the Dionex CTC-1 Trap Column
Using a pump other than the carrier pump, the Dionex CTC-1 should be regenerated and
rinsed using the following two steps.
3.7.4

Neutralization Mode Operation

For detailed operation of Dionex IC systems, consult the accompanying Product Manuals.
!
The Dionex ERS 500 suppressor is not recommended for use with the Neutralization Mode
of Operation. The Dionex ERS 500e should be used.
NOTE
Neutralization pretreatment coupled with Ion Chromatography involves the following steps:
A. The concentrated sample is loaded into the sample loop of the "Sample Valve" with
B. The sample loop is switched in-line and flushed with a stream of deionized water.
C. The deionized water pushes the sample through the Dionex ERS 500, where
D. Common bases or acids that have been diluted fourfold or more can be completely
E. For more concentrated bases or acids or faster flow rates, multiple passes through the
F. The partially neutralized sample is passed down to the 2 mL collection loop on the
G. The recycle valve is actuated so that the sample flow is directed to pass through the
H. The Recycle Valve can be actuated multiple times if multiple passages are necessary
Thermo Scientific
031956-11
2. Rinse the Dionex ATC-HC with deionized water for 30 minutes at a flow rate of 1
mL/min. If the carrier solution is different from water, equilibrate it to the carrier
solution for 30 minutes at a flow rate of 1 mL/min.
1. Regenerate the Dionex CTC-1 with 0.5 M H
mL/min.
2. Rinse the Dionex CTC-1 with deionized water for 30 minutes at a flow rate of 1
mL/min. If the carrier solution is different from water, equilibrate it to the carrier
solution for 30 minutes at a flow rate of 1 mL/min.
either a syringe or an Autosampler.
neutralization begins.
neutralized after one pass through the Dionex ERS 500e at flow rates ≤ 0.5 mL.min.
Dionex ERS 500e will be required for complete neutralization.
Recycle Valve.
Dionex ERS 500e a second time (or multiple times) to neutralize the sample
completely.
for complete neutralization.
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
SO for 50 minutes at a flow rate of 1
2 4
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