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1
– Introduction
!
The AutoSuppression Recycle Mode is not compatible with eluents containing Borate or
Organic Solvents that tend to oxidize easily, such as methanol.
NOTE
1.2.3
The AutoSuppression External Water Mode
The AutoSuppression External Water Mode is used for any application requiring organic
solvents in the eluent or sample, or for applications using borate as the eluent ion. This is the
preferred method of operation for the Dionex ERS 500e. The Dionex ERS 500 and Dionex
AERS 500 Carbonate are compatible with this mode, but not with applications requiring
organic solvents in the eluent, or for applications using borate as the eluent ion. This mode
uses a constant source of deionized water from a pressurized bottle or other source of deionized
water that delivers 0.25 to 2 mL/min for 2 mm applications and 1.0 to 5 mL/min for 4 mm
applications, although the eluent flow rate is typically recommended as double the regenerant
flow rate. The amount of water flowing through the regenerant chambers is independent of the
eluent flow rate. The AutoSuppression External Water Mode eliminates the potential for build-
up of contaminating ions resulting from the oxidation of solvents. It is also recommended when
pursuing analysis with high concentrations of matrix ions. Any analysis performed using the
AutoSuppression Recycle Mode can also be performed using the AutoSuppression External
Water Mode. See
1.2.4
The Chemical Suppression Mode
The Dionex ERS 500 cannot be used in the Chemical Suppression Mode. The peak response
will not be maintained to a constant level in this mode. Thermo Scientific Dionex recommends
the use of a Chemically Regenerated Suppressor (Dionex CRS 500 or equivalent) for chemical
suppression applications. Please refer to the Dionex CRS Manual, Document No. 031727, and
the DCR Kit Manual, Document No. 031664.
The Dionex AERS 500 Carbonate and Dionex ERS 500e can be used in the Chemical
Suppression Mode; however the total suppression capacity is reduced compared to a
Chemically Regenerated Suppressor. Thermo Scientific recommends the use of a Chemically
Regenerated Suppressor such as the Dionex CRS 500 for chemical suppression applications.
1.2.5
The MPIC Suppression Mode
1.2.5.1
Anion MPIC
The Dionex AERS 500e is used for eluent suppression of Mobile Phase Ion Chromatography
(MPIC or ion-pairing) eluents by using the MPIC Suppression Mode. The Dionex AERS 500
and Dionex AERS 500 Carbonate are not recommended for this mode. The MPIC
Suppression Mode is a combination of the AutoSuppression External Water Mode augmented
with a chemical regenerant such as sulfuric acid (H
applied current and a constant source of dilute sulfuric acid solution from a pressurized bottle
delivery system. This mode must be used for MPIC applications requiring an ion pair reagent
and organic solvents in the eluent. The MPIC Suppression Mode reliably provides suppression
of typical eluents for MPIC applications using suppressed conductivity detection. The ion pair
reagents, such as tetrabutylammonium hydroxide (TBAOH), are used in concentrations
typically ranging from 1.0 to 5.0 mM. See
1.2.5.2
Cation MPIC
The Dionex CERS 500e is used for eluent suppression of MPIC eluents by using the
AutoSuppression External Water Mode or the MPIC Suppression Mode depending on the
specific MPIC application. The Dionex CERS 500 is not recommended for this mode. The
MPIC Suppression Mode uses an applied current and a constant source of dilute boric acid
Thermo Scientific
031956-11
Section 3
for complete operating instructions.
Product Manual for the ERS 500 Suppressor
For Research Use Only. Not for Use in Diagnostic Procedures.
SO
). The MPIC Suppression Mode uses an
2
4
Section 3
for complete operating instructions.
Page 17 of 84
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