Summary of Contents for Thermo Scientific Dionex ERS 500
Page 1
Dionex ERS 500 Suppressor 031956 Revision 11 • January 2017 For Research Use Only. Not for Use in Diagnostic Procedures.
Page 2
Dionex Anion Electrolytically Regenerated Suppressor 500 for Carbonate Eluents (Dionex AERS 500 Carbonate (4 mm), Item # 085029) (Dionex AERS 500 Carbonate (2 mm), Item # 085028) Thermo Scientific Product Manual for the ERS 500 Suppressor Page 2 of 84 031956-11...
Page 3
Revision History: Revision 08, April 24, 2013, Rebranded for Thermo Scientific. Product name changed from Dionex SRS 300 to Dionex ERS 500. Revision 09, November, 2013, Updated External Water Mode flow rate recommendations, Updated hydration procedure.
Page 4
Highlights information necessary to prevent damage to software, loss of data, or invalid test IMPORTANT results; or might contain information that is critical for optimal performance of the system. Highlights helpful information that can make a task easier. Thermo Scientific Product Manual for the ERS 500 Suppressor Page 4 of 84 031956-11...
2.2.1 Dionex Reagent-Free Controller (Dionex RFC) ................23 Dionex SCC-10 Suppressor current controller (Item # 074053) ..........23 Back Pressure Coils for the Dionex ERS 500, Dionex ERS 500e and Dionex AERS 500 Carbonate ....................24 2.4.1 Assembly ............................24 Gas Separator Waste Tube for the Dionex ERS 500 ..............
Page 6
3.7.3 Pump Trap Column Installation and Regeneration ................. 41 3.7.4 Neutralization Mode Operation ..................... 42 Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate Storage......43 3.8.1 Short Term Storage (3 to 7 days) ....................43 3.8.2 Long Term Storage (More than 7 days) ..................43 Example Neutralizer Mode Applications................
Page 7
Dionex CERS 500e (4 mm and 2 mm) ................76 Current Settings for Older Detectors ..................79 Appendix B – Suppressor Controller ..................80 Dionex ERS 500 and Dionex ERS 500e Control for the CD20/CD25/CD25A and ED40/ED50/ED50A (Dionex DX-500 instruments) ............80 B.1.1 Dionex ERS 500 Control for the Dionex DX-120 ................
By neutralizing the matrix ions, trace anionic or cationic species can be detected in strong acid or base samples. Thermo Scientific Product Manual for the ERS 500 Suppressor Page 8 of 84 031956-11 For Research Use Only.
2 mm and 4 mm formats for use with 2, 3, 4, or 5 mm Ion Chromatography columns and systems. The 2 mm Dionex ERS 500, Dionex ERS 500e and Dionex AERS 500 Carbonate are specially designed with reduced internal volume to ensure optimum performance with 2 mm columns and systems.
Page 10
346-6390. Outside the U.S., call the nearest Thermo Fisher Scientific office. The Dionex ERS 500 design comprises of three channels defined by two ion exchange membranes. The central channel is the eluent channel and the two side channels are regenerant channels.
Page 11
In the Dionex ERS 500 the eluent channel is filled with ion exchange resin and provides a static capacity which is particularly useful when eluent is pumped into the device with the power off.
Page 12
500e) is optimized for use in the external water mode. The Dionex ERS 500e hardware design is similar to the Dionex ERS 500 hardware design from a sealing perspective and is optimized for minimal band dispersion and improved flow performance. The Dionex ERS 500e uses the same ion exchange membranes, screens, and resin materials as the standard Dionex ERS 500 suppressor.
This design results in decreased gas production at the outlet, and as a consequence, results in less variation in the suppressed background and is able to achieve low noise. As shown in Thermo Scientific Product Manual for the ERS 500 Suppressor Page 13 of 84 031956-11...
Page 14
Thermo Scientific Product Manual for the ERS 500 Suppressor Page 14 of 84 031956-11 For Research Use Only.
MPIC Suppression Mode Neutralization Mode The Dionex ERS 500 is not compatible with the Chemical Suppression Mode. The Dionex CRS 500 or equivalent should be used when Chemical Suppression Mode is required. NOTE Thermo Scientific Product Manual for the ERS 500 Suppressor...
Samples containing Solvents Pairing Reagent) The Dionex ERS 500 is not recommended for External Water Mode; the Dionex ERS 500e is recommended for External Water Mode applications. The Dionex ERS 500 does not support Chemical Suppression Mode. Use of a Chemically Regenerated Suppressor such as the CRS 500 or equivalent is recommended.
This is the preferred method of operation for the Dionex ERS 500e. The Dionex ERS 500 and Dionex AERS 500 Carbonate are compatible with this mode, but not with applications requiring organic solvents in the eluent, or for applications using borate as the eluent ion.
1.2.6 The Neutralization Mode The Dionex ERS 500 (4 mm) when operated in this mode requires a constant deionized water flow of 3 to 5 mL/min through the regenerant chambers. The regenerant water can be delivered from a pressurized bottle or pump. If using a pressurized bottle, it is recommend to use the Dionex ERS 500e.
1.3 Shipment and Storage 1.3.1 Shipment The Electrolytically Regenerated Suppressors (Dionex ERS 500, Dionex ERS 500e, and Dionex AERS 500 Carbonate) should not be subjected to temperatures above 50°C for long durations during shipment, storage or operation, or for any duration above 80°C.
Dionex SRS series of suppressors, such as the Dionex SRS I, Dionex SRS II, Dionex SRS ULTRA, Dionex SRS ULTRA II and Dionex SRS 300. The Dionex ERS 500 can be used in place of any of these suppressors where recycled eluent mode is employed.
Page 21
Section 2.6.1. It is highly recommended to set the current setting for the Dionex ERS 500 or Dionex AERS 500 Carbonate to the exact calculated current (within 1 mA). Older power supplies do not have the 1 mA graduated control. Failure to set the current accurately could reduce...
– Installation The Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate is installed in the column compartment or detector compartment of the chromatography module immediately after the analytical column and before the conductivity detector cell. All components required for installation of the suppressor are included with the system.
Dionex CR-TC Continuously Regenerated Trap Column. Current is delivered at 1 mA resolution. If an ERS Mode is not available, select the equivalent SRS Mode on the power supply to support the Dionex ERS 500 suppressor. The Dionex ERS 500 and Dionex ERS 500e are fully compatible with SRS settings. NOTE The Dionex AERS 500 Carbonate suppressor has different electrical current requirements than the Dionex AERS 500 suppressor.
0.005” (Red) 1.0 ft. The correct amount of back pressure for optimum operation is 40 psi. Back pressure over 450 psi after the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate can cause irreversible damage. CAUTION Thermo Scientific...
2.5 Gas Separator Waste Tube for the Dionex ERS 500 The Gas Separator Waste Tube (Item # 045460) is an integral part of the Dionex ERS 500 system. It separates any electrolytic gases generated in the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate during electrolysis.
Page 26
50, 100, 300 or 500 mA. A lower flow rate requires a lower current. A 2 mm Dionex ERS 500 or Dionex ERS 500e should NEVER be operated at a current above 100 mA. A 2 mm Dionex AERS 500 Carbonate should NEVER be operated at a current above 30 mA.
Page 27
– Installation Table 5 Maximum Suppression Capacity for Dionex ERS 500 and Dionex ERS 500e Suppressor Flow Rate (mL/min) Maximum Suppression Capacity ≤ 200 µ equivalents Dionex AERS 500 4 mm 0.5 - 3.0 Dionex AERS 500 2 mm 0.25 - 0.75 <...
Orient the suppressor with the ELUENT IN port and the cable at the top if installed vertically; align the slots on the back of the Dionex ERS 500 with the tabs on the panel. Press in, and then Thermo Scientific...
– Operation down to lock the Dionex ERS 500 in place. Lift up and pull out to remove the suppressor. Ensure the suppressor is plumbed properly according to the selected mode of operation. Refer to 2, “Installation,” for complete installation instructions.
Valve Valve 4. The correct operating pressure range for the backpressure coil being tested is – P Dionex ERS 500 P = 30–40 psi If the pressure is greater than 40 psi, then trim the back pressure coil and Cell repeat step 2 and 3 to achieve 30-40 psi in step 4.
Only use the AutoSuppression Recycle Mode for eluents and samples without organic solvents or metallic contaminants such as iron in ground water. CAUTION Thermo Scientific Product Manual for the ERS 500 Suppressor Page 31 of 84 031956-11...
Avoid adding dead volume to the system by keeping all eluent lines as short as possible. A. Install the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate inside the Dionex ICS Module. B. Connect the outlet of the analytical column to the ELUENT IN of the suppressor...
Page 33
Refer to Section 2.6.1 suppressor current calculations and Appendix A for examples. Thermo Scientific Product Manual for the ERS 500 Suppressor Page 33 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.
Recycled Eluent Mode unless organic solvents or metallic contaminants are present in the eluent or sample, or borate is used as the eluent. NOTE Thermo Scientific Product Manual for the ERS 500 Suppressor Page 34 of 84 031956-11 For Research Use Only.
4 mm systems. Avoid adding dead volume to the system by keeping all eluent lines as short as possible. A. Install the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate in the first slot inside the Chromatography Module.
10 –15 psi for the Dionex ERS 500, or 5 – 10 psi for the Dionex ERS 500e or Dionex AERS 500 Carbonate suppressor. Please note that this value is highly system dependent and may vary from one suppressor to the next.
3.5 Plumbing for Chemical Suppression Mode Operation The Dionex ERS 500 is not compatible with the chemical suppression mode. Therefore Thermo Scientific Dionex recommends the Dionex CRS 500 or equivalent for chemical suppression mode applications.
Anion MPIC separations and the current level and regenerant flow rate required to suppress them. The Dionex ERS 500 suppressor is not recommended for use with the MPIC Suppression Mode of Operation. The Dionex ERS 500e should be used.
Page 39
MPIC separations and the current level and regenerant flow rate required to suppress them. The Dionex ERS 500 suppressor is not recommended for use with the MPIC Suppression Mode of Operation. The Dionex ERS 500e should be used.
For detailed information on installation of the Dionex ERS 500e on Dionex ICS systems for Neutralization Mode, refer to Thermo Scientific Dionex Application Notes 93 and 94. The Dionex ERS 500 suppressor is not recommended for use with the Neutralization Mode of Operation. The Dionex ERS 500e should be used.
2. Using a coupler (Item # 039056) and a 1/8” OD piece of tubing (Item # 035728) from the AutoNeutralization Ship Kit, connect one end of this line to the REGEN OUT port of the Dionex ERS 500 and then connect the other end of the line to the Gas Separator Waste Tube.
3.7.4 Neutralization Mode Operation For detailed operation of Dionex IC systems, consult the accompanying Product Manuals. The Dionex ERS 500 suppressor is not recommended for use with the Neutralization Mode of Operation. The Dionex ERS 500e should be used. NOTE Neutralization pretreatment coupled with Ion Chromatography involves the following steps: A.
The trace ions are then analyzed by the Dionex Ion Chromatography system. For detailed information on the timing and configuration of the Sample Valve, Recycle Valve, and Injection Valve, refer to Thermo Scientific Dionex Application Note 93 or 94, or to the Dionex SP10 AutoNeutralizer Operator’s Manual (Document Number 034980).
Dionex ERS 500e. The use of other concentrators may result in excessive back pressure to the Dionex ERS 500e, thus damaging the Dionex ERS 500e. The Dionex ERS 500 suppressor is not recommended for use with the Neutralization Mode of Operation. The Dionex ERS 500e should be used.
The additional of complexing agent such as pyridine-2,6-dicarboxylic acid (PDCA, Item # 039671) to the CS12A eluent is required to selectively remove transition metals from the eluent via the suppression system. Thermo Scientific Product Manual for the ERS 500 Suppressor Page 45 of 84 031956-11 For Research Use Only.
* n = 8 , “Dionex IonPac CS12A Isocratic Analysis of 24% Sulfuric Acid”. Separation Conditions are shown in Section 4. Thermo Scientific Product Manual for the ERS 500 Suppressor Page 46 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.
0.5-5.0 mM NaOH in 3.5 min 5.0-38.25 mM NaOH in 12 min 18.0 The steps for sample neutralization can be performed while the Thermo Scientific Dionex IonPac AS11 is equilibrating with the starting eluent (90% E1/10% E2). NOTE Seven minutes are required at the beginning of the above program for equilibration of the Dionex IonPac AS11 with E1 prior to injecting the next sample.
With Guard: 1,850 psi (12.75 MPa) Figure 8 Dionex IonPac AS11 Gradient Analysis of Blank, Standard, 10% NaOH Sample and Spiked 10% NaOH Sample Thermo Scientific Product Manual for the ERS 500 Suppressor Page 48 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.
Page 49
2.112 29. cis-Aconitate 2.000 2.000 1.512 30. trans-Aconitate 2.000 0.072 2.072 2.303 ND = None Detected Thermo Scientific Product Manual for the ERS 500 Suppressor Page 49 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.
14–16 µS Figure 5 Dionex IonPac AS12A Isocratic Analysis of Blank, Standard, 25% NaOH Sample and Spiked 25% NaOH Sample Thermo Scientific Product Manual for the ERS 500 Suppressor Page 50 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.
Page 51
0.100 0.134 + 0.028 0.234 0.238 + 0.022 Based on 8 runs ND = None Detected Thermo Scientific Product Manual for the ERS 500 Suppressor Page 51 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.
50 mN H Expected Background Conductivity: 14–16 µS Figure 6 Dionex IonPac AS12A Analysis of 20% Ammonium Hydroxide Thermo Scientific Product Manual for the ERS 500 Suppressor Page 52 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.
50 mN H Expected Background Conductivity: 14–16 µS Figure 7 IonPac AS12A Analysis of 25% Tetramethylammonium Hydroxide Thermo Scientific Product Manual for the ERS 500 Suppressor Page 53 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.
50 mN H Expected Background Conductivity: 14–16 µS Figure 8 Dionex IonPac AS12A Analysis of 25% Tetrabutylammonium Hydroxide Thermo Scientific Product Manual for the ERS 500 Suppressor Page 54 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.
8. Calcium 50.0 Figure 9 Dionex IonPac CS12A Blank and Standard Analysis Figure 14A Figure 14B Thermo Scientific Product Manual for the ERS 500 Suppressor Page 55 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.
10.6 + 0.1 30.6 33 + 1 Based on 8 runs ND = None Detected, B = Blank Thermo Scientific Product Manual for the ERS 500 Suppressor Page 56 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.
5. Trimethylamine 6. Magnesium 7. Calcium 133.0 Figure 11 Dionex IonPac CS12A Analysis of 25% Acetic Acid Thermo Scientific Product Manual for the ERS 500 Suppressor Page 57 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.
7. Magnesium 15.3 8. Calcium 59.6 Figure 126 Dionex IonPac CS12A Analysis of 10% Hydrofluoric Acid Thermo Scientific Product Manual for the ERS 500 Suppressor Page 58 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.
6. Magnesium 22.4 7. Calcium 144.1 Figure 13 Dionex IonPac CS12A Analysis of 22% Phosphoric Acid Thermo Scientific Product Manual for the ERS 500 Suppressor Page 59 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.
The purpose of the Troubleshooting Guide is to help you solve operating problems that may arise while using the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate. For more information on problems that originate with the Ion Chromatograph System or the specific exchange column set in use, refer to the Troubleshooting Guide in the appropriate Product Manual.
– Troubleshooting Guide 5.2 Small or Increasing Analyte Peak Areas This problem is caused by running eluent through the Dionex ERS 500, Dionex ERS 500e, or Dionex AERS 500 Carbonate with the power off while using the AutoSuppression Recycle Mode or the AutoSuppression External Water Mode. It may also be caused by application to too little current to the suppressor for an extended period of time.
5.2.2 Suppressor Electrolytic Regeneration Steps A. The Dionex ERS 500, Dionex ERS 500e and Dionex AERS 500 Carbonate are unique that they can be regenerated electrolytically. Although this is slower than Chemical Regeneration, it does not require the suppressor to be disconnected or the use of any reagents.
Page 63
NOTE Do not use the Analytical pump for regeneration purpose as this would contaminate the pump NOTE Thermo Scientific Product Manual for the ERS 500 Suppressor Page 63 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.
– Troubleshooting Guide 5.3 High Background Conductivity A. Check the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate current settings. Refer to the suppressor current settings section. B. Check for eluent flow out of the suppressor ELUENT OUT port.
Page 65
In the above figure, a bubble is released from ELUENT IN line by loosening and tightening the fitting at 56.6 minutes into the run. Noise: ~2.5 nS before < 0.5 nS after Thermo Scientific Product Manual for the ERS 500 Suppressor Page 65 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.
A. Check for leaks throughout the system. If a fitting is leaking, tighten it carefully until the leak stops. Do not over tighten. If the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate is observed to be leaking from the center or bottom 5.8, “Liquid Leaks.”...
Dionex ERS 500 into the housing and check the back pressure after the suppressor. B. If there is liquid coming out of any of the four ports of the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate, carefully tighten the fittings in the leaking port –...
Refer to Section 3.3.3 Installation in thermal chamber. 20°C is the optimum temperature for Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate operation. E. Large changes to the flow rate or applied current of the suppressor: When changing eluent concentration or flow rate, recalculate the optimum current using the attached calculator;...
D. If cleaning the suppressor membranes does not restore neutralization efficiency, the suppressor may need to be replaced. E. If you cannot solve the problem on your own, contact the Thermo Scientific Regional Office nearest you. 5.12 Low Neutralization Capacity (Neutralization Mode Only) For the Dionex AERS 500e this problem is caused when the ion exchange sites in the suppressor are converted from the hydronium form to the salt form.
Dionex AERS 500 Carbonate Suppressor Cleanup This section describes routine cleanup procedures for the Dionex Electrolytically Regenerated Suppressors (Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate) in the case Section 5, “Troubleshooting Guide”) of contamination. Consult the Troubleshooting Guide (see to first determine that the system is operating properly.
A. Turn off the ERS Control or SRS Control unit and system pump. B. Disconnect the analytical (and guard) column(s) from the injection valve and the Dionex ERS 500, Dionex ERS 500e or Dionex AERS 500 Carbonate. Refer to the specific analytical column Product Manual for column cleanup procedures.
Water Mode is the mode used if organic solvents (up to 40%) are present in the eluent. It eliminates the potential for buildup of contaminating ions resulting from the oxidation of solvents. Thermo Scientific Product Manual for the ERS 500 Suppressor Page 72 of 84 031956-11 For Research Use Only.
Page 73
For optimum performance it is recommended to use a Dionex RFC-10 or Dionex RFC-30 power supply to provide fine current control with the optimum setting. Thermo Scientific Product Manual for the ERS 500 Suppressor Page 73 of 84 031956-11 For Research Use Only.
Page 74
0.30 – AS25 36 mM OH 0.25 – AS26 55 mM OH 0.30 3 mm format Thermo Scientific Product Manual for the ERS 500 Suppressor Page 74 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.
External Water Mode is the mode used if organic solvents (up to 40%) are present in the eluent. It eliminates the potential for buildup of contaminating ions resulting from the oxidation of solvents. Thermo Scientific Product Manual for the ERS 500 Suppressor Page 75 of 84 031956-11 For Research Use Only.
The AutoSuppression External Water Mode is the mode used if organic solvents (up to 40%) are present in the eluent. It eliminates the potential for buildup of contaminating ions resulting from the oxidation of solvents. Thermo Scientific Product Manual for the ERS 500 Suppressor Page 76 of 84 031956-11 For Research Use Only.
Page 77
For optimum performance it is recommended to use a Dionex RFC-10 or Dionex RFC-30 power supply to provide graduated current control with the optimum setting. Thermo Scientific Product Manual for the ERS 500 Suppressor Page 77 of 84 031956-11 For Research Use Only.
Page 78
0.5 – 4 mL/min Dionex CERS 500e (2 mm) equal to 2x eluent flow rate Measured with power ON using a graduated cylinder Thermo Scientific Product Manual for the ERS 500 Suppressor Page 78 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.
The readout is the approximate current level. For optimal performance Dionex recommends the use of a Dionex RFC-10 or Dionex RFC-30 power supply. NOTE Thermo Scientific Product Manual for the ERS 500 Suppressor Page 79 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.
NOTE To operate the Dionex ERS 500 or Dionex ERS 500e from the front panel of these detectors: A. Make sure the detector is in one of the main conductivity screens by choosing “Main Screen” or “Detail Screen.” Press the “Menu” button until you see this as an option.
The Dionex RFC-10 provides current at 1 mA intervals for exact current control. NOTE This unit is intended to provide power and control to one Dionex ERS 500 or Dionex ERS 500e when the associated detector does not have ERS control integrated into it.
Page 82
C u rre nt SRS CONTROLLER There is no “ERS” setting on the Dionex SRC-1, use the SRS setting. The Dionex ERS 500 and Dionex ERS 500e are fully backwards compatible with all SRS power adapters and settings. For optimal performance a Dionex SCC-10 suppressor current controller should be...
Page 83
Control”). This is the to the Dionex SRC-1 on and off (see only way to turn the current output to the Dionex ERS 500 on and off on the Dionex SRC-1. Two line input fuses are located in the fuse drawer on the socket of the AC input connector on the back panel of the Dionex SRC-1.
C.1.2, “The Alarm Red LED” lowering the power setting on the front panel. Also, see Section for other causes. Thermo Scientific Product Manual for the ERS 500 Suppressor Page 84 of 84 031956-11 For Research Use Only. Not for Use in Diagnostic Procedures.