Default Generic Panels And Protocols - Beckman Coulter Navios Instructions For Use Manual

Application Flow-Set Pro Protocol
Name
AS 6C 2L AS 6C 2L_STAND.Pro
The initial protocol in each application, which you use to adjust or confirm correct PMT High
Voltage and parameter gain settings, utilizes Flow-Set Pro fluorospheres.
If you wish to use a negative control sample use the "AS *_STAND.Pro" protocol in the above panels.
You must modify the protocol for the number of colors you are analyzing.
Each AutoSetup II application requires a user defined base protocol with the desired parameter
names to generate the application. The Application Definition Wizard creates a unique base
protocol with the same name as the application.
The guidelines below show approximate region widths generated by the Application Definition
Wizard. Flow-Set Pro region widths generated by the Application Definition Wizard may be edited.
If different regions widths are desired, open the appropriate *_Stand protocol onto the workspace
and edit the regions. Save the protocol. Subsequent scheduling of the application uses the edited
regions.
• Forward Scatter (linear): ±20
• Side Scatter (linear): ±50
• Fluorescence Parameters (logarithmic);
• 1st decade - beginning or low end: ±0.1
• 1st decade - high end: ±0.3
• 2nd decade - beginning or low end: ±1.0
• 2nd decade - high end: ±3.0
• 3rd decade - beginning or low end: ±10
• 3rd decade - high end: ±30
• 4th decade - beginning or low end: ±100
• 4th decade - high end: ±300

Default Generic Panels and Protocols

The following generic protocols are included with Navios software.
Panel Name
773232AH
Compensation
Protocols
AS 6C 2L_FL01.Pro
AS 6C 2L_FL02.Pro
AS 6C 2L_FL03.Pro
AS 6C 2L_FL04.Pro
AS 6C 2L_FL05.Pro
AS 6C 2L_FL06.Pro
Protocol
Verification Protocol Settings Protocol
AS 6C 2L_VERIFY.Pro AS 6C 2L
Settings.Pro
Specifications 4
Software Specifications
Base Protocol
AS
6C2L_APP.Pro
4-9