Red Laser Beam Spot Size; Violet Laser Beam Spot Size; Laser Beam Separation; Optical Filters - Beckman Coulter Navios Instructions For Use Manual

Red Laser Beam Spot Size

An elliptical spot 9.6 μm high by 72 μm wide.

Violet Laser Beam Spot Size

An elliptical spot 8.9 μm high by 70 μm wide.

Laser Beam Separation

The laser beams are 125 μm (±12.5 μm) apart.

Optical Filters

The filters used in the Navios system are dependant upon your system configuration. See
Figure 3.5 and

Sensors

• The FS sensor and the SS sensor are photodiodes.
• The FL sensors are photo-multiplier tubes (PMTs) that have a 200-nm to 800-nm spectral range.

Signal Processing

• High voltage amplification, minimum 250 up to 1,100, in increments of 1, for FL1-FL10.
• Vernier gain (fine amplification), up to 1,000 (labeled volts), in increments of 1, for FS and SS. A
change of 1 to 750 represents a 1-to-4 change in gain:
• Linear amplification (gain) by 1.0, 2.0, 5.0, 7.5, 10, 20, 50, 75, 100, 200, 500 or 750 for FS and SS.
• Linear amplification (gain) by 1.0 or 2.0 for FL1-FL10.
• Four-decade digital logarithmic transformation of FS, SS and FL1-FL10.
Note: A scale of 0.1 to 1,000 is displayed on the plot axis for logarithmic parameters, but the
default statistics are based on an actual scale of 0.1024 to 1024. The displayed scale can be
changed to 1 to 10,000.
•
Six decade with True View enabled. Data is transformed in a 'log' style at high values, but
'linearly' near the axis. Up to 2 negative decades of data may be displayed. Transform 'reflects'
around origin and becomes Logarithmic at high negative values.
• Fluorescence color compensation is available in 0.1 increments, from 0 to 100%, for FL1-FL10.
• A discriminator (maximum value of 1,000) is available for any one of the signals. Only one
discriminator can be specified for any one sample acquisition.

Dynamic range

20-bit data acquisition
773232AH
Figure 3.6.
Instrument Specifications And Characteristics
Specifications 4
Figure 3.4,
4-3