Plot Display; Prism - Beckman Coulter Navios Instructions For Use Manual

Plot Display

The results of sample analysis appear on the Workstation screen as graphs called plots. You assign
the parameters to the plot axes. Plots can be displayed in black and white or color as:
• Single-parameter histogram plot
• Dual-parameter dot plot
• Dual-parameter density plot
• Prism plot
Dual-parameter plots can be displayed in 64 x 64, 128 x 128, 256 x 256, or 512 x 512 resolution. Plots
can be displayed on a black or white background.

PRISM

Prism is used to analyze multicolor immunofluorescence samples. With multicolor
immunofluorescence a cell is either positive or negative for each of up to ten cell surface markers.
A particular combination is called a phenotype. Prism allows you to display percentages on all
phenotypic populations in a single plot. It is software derived and can be acquired in either run time
or listmode.
Prism is available on up to ten parameters. TIME, RATIO, and Prism itself cannot be used for Prism.
All other signals can be used for Prism. Generally, the fluorescence parameters are used.
A Prism plot shows a spike or population for each antibody combination with a percent of the total
that represents the percent of the total events in the Prism plot. See
Figure 3.11 Prism Plot
See Prism Plot
773232AH
for instructions on using Prism.
Operation Principles
Plot Display
Figure 3.11.
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