Third Laser; Reagents And Quality Control Materials; Sheath Fluid; Cleaning Agent - Beckman Coulter Navios Instructions For Use Manual

Use And Function

Reagents And Quality Control Materials

Third Laser

A 405 nm violet solid-state laser providing a minimum of 40 mW light regulated laser power.
Reagents And Quality Control Materials
Beckman Coulter recommends these reagents or their equivalents. All stated analytical
characteristics and specifications in this manual are based on the use of the Navios Flow Cytometry
System with the following reagents.

Sheath Fluid

In the Cytometer, the sample is guided into a stream of sheath fluid to make the sample cells flow
single file through the laser beam. IsoFlow sheath fluid, a nonfluorescent, balanced electrolyte
solution, is made for this purpose.
IsoFlow sheath fluid has the following characteristics:
• Filtered to 0.2 μm
• Transparent and nonfluorescent to 488-nm, 405-nm and 638-nm laser light
• Low background
• Compatible with the characteristics of the sample being measured (such as pH, osmolality,
conductivity).
The internal sheath container has a working capacity of aproximately 500 mL. The amount of sheath
fluid the container holds beyond the working capacity is for pressurization and level sensing.

Cleaning Agent

When the Cytometer is in the Cleanse mode, FlowClean cleaning agent flushes sample tubing and
helps to reduce protein buildup and particles in the instrument. Each cleanse cycle uses about 15 mL
of cleaning agent.
Read the container's label for more information on the cleaning agent.

Quality Control Materials

The quality control materials available from Beckman Coulter are:
Flow-Check Pro
Fluorospheres
Flow-Set Pro Fluorospheres
Flow-Count Fluorospheres
1-4
Fluorospheres used to check the stability of the optical and fluidic systems.
Fluorospheres used to standardize light scatter and fluorescence intensity.
Fluorospheres with an assayed concentration that are used to determine
absolute counts.
773232AH