Volts; Gain; Details; Setup Mode - Beckman Coulter Navios Instructions For Use Manual

Using Navios Software 10
Cytometer Menu
Discriminators
Lists the currently selected parameter and allows the setting and modification of discriminator
values. To change a value, select the discriminator you wish to alter and use the Slide Control on the
right hand part of the display to change the value as appropriate.

Volts

Select the appropriate Voltage for the particular Detector. This is done by using the Slide Bar on the
right side of the screen and adjusting it to the required value. For fine adjustment, the Up and Down
arrows at the ends of the Slide Bar can be used.

Gain

Adjusts the Gain amplifier. Set the Gain to the appropriate setting you require for that Detector. This
is chosen from the drop down box, which appears when you click in the box.

Details

Display the Laser Control dialog.
NOTE
Do not use an AutoGate as a Live Gate. A Live Gate should be exclusionary.

Setup Mode

Enable this checkbox to allow the continuous acquisition of data from the Cytometer. In this mode,
Stops are disabled, Printing is disabled and listmode files are not stored.
Setup Mode allows continuous acquisition of data from the Cytometer giving continuous real-time
feedback of instrument performance on the displayed plots. Any Stop & Save values set are ignored
in this mode.
Abort Setup Mode
Display of data continues until is selected, to end data acquisition, or the box is
unchecked, to begin normal data acquisition.
In Setup Mode the plots are updated real-time with only the most recently acquired events
displayed. The incoming data is not saved. During Setup Mode, older events are cleared from the
screen and from memory allowing the Cytometer settings to be adjusted in real-time without
reaching a set stop condition.
Data is displayed on a "first in; first out" basis. The number of events to be displayed can be changed
by entering the required value into the

Dots

input box.
Abort Setup Mode
Once you have finished using Setup Mode click , or uncheck the checkbox and then
perform the acquisition.
Dots
This sets the maximum events to be displayed during Setup Mode . The default size is 1,000 events.
Setup Mode
The rolling displays are updated up to three times per second. To change the size, edit
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