Disconnecting The Bag From The Filler And Sealing The Bag; Analyzing The Samples; Analysis Setup; Noa Setup - GE MOA 280i Operation And Maintenance Manual

Disconnecting the bag from the filler and sealing the bag.

After the subject has completed the maneuver:
• Stand the bag filler on the inspiratory filter.
• Loosen the nut on the filler and remove the bag. The self-sealing valve will
close and seal the bag. Do not use caps to seal the bags.
Repeat the above procedures to connect another bag to the filler for replicate
measurements. Normally, subjects will fill three bags, and the average NO
concentrations in these three samples is used for the subject's FE Subjects
NO, 0.05.
should rest a few minutes between exhalations.
After the subject has filled the replicate bags, remove the mouthpiece and filter.
The filter should be discarded, while the mouthpiece can be reused after
sterilization.

Analyzing the Samples

The same procedure is used to analyze samples collected using either the vital
capacity or deadspace discard bag kits. The NOAnalysis Bag program is designed
for collecting data during analysis of these samples (consult the NOAnalysis manual
for instructions). Alternatively, the NOA's front panel display can be used for
viewing the concentration of NO in the samples.

Analysis Setup

The setup for the analysis of Bag samples is shown in Figure 8-5 and consists of a
three-way valve, a male-male Luer adapter, the zero air filter and the gas-
sampling package. Connect the Luer adapter to the outlet of the zero air filter and
to one leg of the three-way valve. Connect the NO sample line to the other female
Luer leg of the three-way valve. The bags will be connected to the male Luer leg
of the valve.

NOA Setup

8-11
GE Analytical Instruments ©2006 DLM 14291 Rev. A