Bio-Tek ELx800 Operator's Manual page 101

Validation Formulas
Validation formulas can be used to determine whether or not blanks
and/or controls are valid. In addition, Assay Validation formulas can be
used to determine whether or not the entire assay should be considered
valid.
See
accessing the SELECT VALIDATION TYPE screen.
S E L E C T
C O N T R O L
Control and Blank Validation Formulas
Blank Validation is used to ensure that the OD values for the blank
replicates, or for the blank mean, meet certain criteria. Control Validation
serves the same purpose as Blank Validation, but it applies to the control
replicates or control mean. If the criteria are not met, results are considered
suspect, and the message "RESULTS INVALID! BLANK (OR CONTROL)
VALIDATION FAILED" appears on results reports.
Examples
If an assay protocol states that
ELx800 Operator's Manual
Formula Type on page 70 for instructions on selecting an assay and
V A L I D A T I O N
One blank validation formula can be defined.
Up to four control validation formulas can be defined.
Define the plate map (via DEFINE
control validation formulas.
Blank/Control validation can be performed on individual replicates
(BLK, PC), or on the group mean (BLK;x, NC;x).
Each blank well on a plate should have an OD of less than 0.050, the
formula is: BLK < 0.050
Each Positive Control replicate must have an OD higher than 1.000,
but less than 2.500, this can be accomplished with one formula:
PC>1.000ANDPC<2.500
Or with two separate formulas:
PC > 1.000 and PC < 2.500
Negative Control mean must have an OD of less than 0.100, the
formula is: NC;x < 0.100
Define (Method, Map, Formula and Curve) |
A S S A Y
MAP) before creating blank or
T Y P E :
B L A N K
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