Nikon LS-4500AF User Manual page 55

6. Using the Film Holder
Step 2
Set the film in the film compartment with the emulsion side downward.
The four frames aligned with the apertures can be scanned.
The fourth image will be read.
The third image will be read.
The second image will be read.
The first image will be read.
Nikon 35
Film Scanner LS-4500AF User's Manual