Nikon ECLIPSE 50i Instructions Manual page 34

10
Switch to the desired objective
and view the specimen.
• Refocus.
• Use the ND filters of the Epi-fl attachment to
adjust brightness.
• Adjust the field diaphragm so that it extends
slightly beyond the field of view.
• When using an oil immersion type objective,
apply immersion oil between the specimen
and the objective.
⇒ P.51
⇒ P.48
11
To return to bright-field
microscopy.
• Close the shutter of the Epi-fl attachment and
block the light emitted by the mercury lamp.
• Turn on the microscope power switch to turn
on the diascopic light source.
• Turn the filter cube switching knob and move
the position without a filter cube into the
optical path.
12
Turn off all power switches after
completing observations.
Chapter 2 Microscopy
2.3 Microscopy with Epi-fl Attachment Mounted
31
Use the ND filters to adjust brightness.
Select the desired objective.
Press the switch to
the "|" position.
Press the power switch to
the "◯" position.
2
Close the
shutter.