Table of Contents
Advertisement
Advertisement
Table of Contents
Related Manuals for Nikon Eclipse 50i POL
Summary of Contents for Nikon Eclipse 50i POL
- Page 1 M362E 05.8.NF.1 Polarizing Microscope ECLIPSE 50i POL Instructions...
-
Page 3: Preface
To ensure correct use, please read this manual carefully before operating the product. • No part of this manual may be reproduced or transmitted in any form without the prior consent of Nikon. • The contents of this manual are subject to change without notice. -
Page 4: Meaning Of Symbols Used On The Equipment
Decontaminate the contaminated portion according to the standard procedure of your facility. Caution for heat This marking on the lamphouse of the Eclipse 50i POL calls your attention on the following; • The lamp and its surroundings (including the lamphouse) become very hot during and immediately after the illumination. -
Page 5: Safety Precautions
Never attempt to disassemble any part other than the parts described in this manual. If you experience problems with the product, contact your nearest Nikon representative. 3. Read the instructions carefully To ensure safety, carefully read this manual and the manuals provided with any other equipment used with this product. -
Page 6: Caution
If water enters a component, immediately suspend use of this product, disconnect the power cord from the outlet, and contact your nearest Nikon representative. 5. Do not place any object on top of the product Do not place any object on top of the product or cover it with a piece of cloth or so on. - Page 7 Safety Precautions CAUTION 7. Cautions as to conditions for operation, transportation, and storage The product must be operated, transported, or stored under the following conditions. Using or storing the product in hot, humid locations may result in mold formation or condensation on lenses, performance degradation, or malfunctions.
-
Page 8: Notes On Handling The Product
Notes on handling the product Notes on handling the product 1. Handle with care This product is a precision optical instrument. Avoid subjecting it to sudden impact and shocks. Even relatively minor impacts are capable of affecting the precision of the objective. 2. - Page 9 Notes on handling the product 6. Focusing knobs • Never turn the focus knobs on the left and right sides of the product in opposite directions at the same time. Doing so may damage the product. • Turning the coarse focus knob past its farthest point will damage the product. Never use excessive force when turning the knob.
-
Page 10: Expressions Used In This Manual
Expressions Used in This Manual The product names and expressions used in this manual are given below. The manual uses the following expressions: Name of device Expressions Microscope Eclipse 50i POL 50i POL P-TB binocular eyepiece tube Binocular eyepiece tube P-TT trinocular eyepiece tube... -
Page 11: Table Of Contents
Contents Contents Preface ........................ 1 WARNING and CAUTION Symbols ................... 1 Meaning of symbols used on the equipment ..............2 Safety Precautions ....................3 WARNING ...................... 3 CAUTION ....................... 4 Notes on handling the product ................. 6 Expressions Used in This Manual ..............8 Part Names.................... - Page 12 Contents 3.4.2 Knob Rotation and Stage Movement............. 34 3.4.3 Number of Knob Turns and Distance of Stage Travel........34 3.4.4 Adjusting the Rotating Torque of the Coarse Focus Knob......... 35 3.4.5 Coarse Focus Stopper Ring ................. 35 Stage Rotation....................36 3.5.1 Stage Rotation ..................
- Page 13 Contents Troubleshooting.................... 27 Optical System ....................65 Mechanical System.................... 67 Electrical System ....................67 Care and Maintenance................... 30 Lens Cleaning ....................68 Cleaning the Product ..................68 Disinfecting the Product ..................68 Storage ......................69 Periodical Inspection (Fee Charged)..............69 Specifications....................
-
Page 14: Part Names
Part Names Components and Controls Front Left Side 1.1.1 C mount adapter (option) TV vertical tube adapter (option) Binocular part Trinocular eyepiece tube Polarizing intermediate tube Analyzer slider P-CL 1/4λ & tint plate *1 Centering nosepiece Microscope main body Objective Vernier Condenser focus knob Stage rotation clamp screw... -
Page 15: Front Right Side
Chapter 1 Part Names 1.1 Components and Controls Front Right Side 1.1.2 Eyepiece Diopter adjustment Optical path selection lever (only for the trinocular eyepiece tube) Bertrand lens centering screws (both sides) Bertrand lens turret Bertrand lens focus ring Microscope main body Specimen holders *2 Circular graduated stage Condenser focus knob... -
Page 16: Microscope With The Epi Illuminator
Chapter 1 Part Names 1.2 Microscope with the Epi Illuminator Microscope with the Epi Illuminator Lamphouse *3 Filter slider Dummy slider Epi illuminator (LV-UEPI) Aperture diaphragm open/close lever Field diaphragm Illumination selection lever centering screws (both sides) (bright-field/dark-field) Field diaphragm open/close lever Polarizer slider or dummy slider... -
Page 17: Rear Side
Chapter 1 Part Names 1.3 Rear Side Rear Side Rear Side of the Microscope 1.3.1 Handle Input voltage indication Lamphouse AC inlet Tool When the Lamphouse Cover Is Opened 1.3.2 Lamphouse cover Halogen lamp... -
Page 18: Microscopy
Microscopy Diascopic Bright-field Microscopy This section describes the diascopic bright-field microscopy using the built-in lamp of the microscope. Turn on the power. When the microscope has power applied to it, the power indicator on the right side of the microscope is lit. -
Page 19: Eyepiece Tube
Chapter 2 Microscopy 2.1 Diascopic Bright-field Microscopy Move the Bertrand lens turret to the “O” position to remove the Bertrand lens from the optical path. ⇒ P.48 Remove the Bertrand lens from the optical path. Move the 10X objective into the optical path. - Page 20 Chapter 2 Microscopy 2.1 Diascopic Bright-field Microscopy Focus on the specimen. Fully loosen the coarse focus stopper ring. ⇒ P.34 Focus on the specimen using the coarse focus knob and the fine focus knob. Adjust the diopters and the interpupillary distance. ⇒...
- Page 21 Chapter 2 Microscopy 2.1 Diascopic Bright-field Microscopy Switch to any desired objective and view the specimen. Switch to any • Each time you change objectives, the field desired objective diaphragm and the condenser aperture diaphragm must be adjusted. For the bright-field microscopy, the field aperture should be adjusted so that its image circumscribes the view field.
-
Page 22: Orthoscopic Observation
Chapter 2 Microscopy 2.2 Orthoscopic Observation Orthoscopic Observation This section describes the orthoscopic observation procedure. This is the characteristic observation method of polarizing microscopes. In this method, the specimen is observed with the polarizer and the analyzer placed in the optical path. The shape of the specimen in the direction of the optical axis and its optical properties in the direction of the thickness can be observed. -
Page 23: Conoscopic Observation
Chapter 2 Microscopy 2.3 Conoscopic Observation Conoscopic Observation This section describes the conoscopic observation procedure. This is the characteristic observation method of polarizing microscopes. In this method, the specimen is observed with the polarizer, the analyzer, and the Bertrand lens placed in the optical path. The specimen can be observed from various angles with diascopic light in the form of a single image. -
Page 24: Episcopic Microscopy (With The Epi Illuminator Option)
Chapter 2 Microscopy 2.4 Episcopic Microscopy (with the Epi Illuminator Option) Episcopic Microscopy (with the Epi Illuminator Option) You can perform the episcopic microscopy with the epi illuminator option. Before the microscopy • If the cumulative lit-on time has exceeded the average operation life for lamps of its kind, replace the lamp. - Page 25 Chapter 2 Microscopy 2.4 Episcopic Microscopy (with the Epi Illuminator Option) Move the Bertrand lens turret to the “O” position to remove the Bertrand lens from the optical path. Remove the Bertrand lens from the optical path. Push in the illumination selection lever on the epi illuminator to set the bright-field position for it.
- Page 26 Chapter 2 Microscopy 2.4 Episcopic Microscopy (with the Epi Illuminator Option) Move the open/close Fully open the field diaphragm and lever to upper position to fully open the field the aperture diaphragm. diaphragm and the aperture diaphragm. Move the NCB 11 filter into the optical path.
- Page 27 Chapter 2 Microscopy 2.4 Episcopic Microscopy (with the Epi Illuminator Option) Focus on the Switch to any desired objective specimen using the and view the specimen. Adjust the coarse focus brightness knob and the with the fine focus • Each time you change objectives, the field ND filters.
-
Page 28: Photomicroscopy
Chapter 2 Microscopy 2.5 Photomicroscopy Photomicroscopy For detailed discussions of the camera, photomicroscopic software, and PC, refer to the operating manuals provided with the respective products. The following instructions assume a DS camera head DS-5M and DS-L1 camera control unit. Adjust the microscope for proper image observation. - Page 29 Chapter 2 Microscopy 2.5 Photomicroscopy Capture and save images. Position the specimen. Readjust the focus. Adjust the image brightness using the camera exposure compensation function. Check the image using the Freeze button. When the image is acceptable, press the CAPT. button to save the image. The operating procedure differs if DF/FL scene mode is selected.
-
Page 30: Individual Operations
Individual Operations Item Title Operating sections Power ON/OFF Power switch Brightness adjustment Brightness control knob, preset switch, ND filter, and color compensating filter Optical path selection (for trinocular Optical path selection lever eyepiece tube) Stage vertical movement Coarse/fine focus knobs, coarse focus torque adjustment knob, and coarse focus stopper ring Stage rotation Circular graduated stage, vernier, and stage rotation clamp... -
Page 31: Power On/Off
Chapter 3 Individual Operations 3.1 Power ON/OFF Power ON/OFF Power Supply for the Microscope 3.1.1 To turn on the microscope, press the power switch to the “I” position. To turn off the microscope, press the power switch to the “O” position. When the power to the microscope is on, the power indicator on the right side of the microscope is lit. -
Page 32: Brightness Adjustment
Chapter 3 Individual Operations 3.2 Brightness Adjustment Brightness Adjustment The brightnesses of images are adjusted with the following methods: Method Operating controls Section Diascopic image Adjusting the lamp voltage (causes Brightness control knob (microscope 3.2.1 color temperature shifts) body) Preset switch, preset brightness 3.2.2 volume-control Setting ND filters (free from color... -
Page 33: Adjustment Using The Preset Switch
Chapter 3 Individual Operations 3.2 Brightness Adjustment Adjustment Using the Preset Switch 3.2.2 Push in the preset switch to enable the brightness level (lamp voltage) previously set with the preset brightness volume-control. Toggle the preset switch – that is to say, return it to the out position –... -
Page 34: Adjustment Using Nd Filters On The Epi Illuminator
Chapter 3 Individual Operations 3.2 Brightness Adjustment Adjustment Using ND Filters on the Epi Illuminator 3.2.5 The LV-UEPI universal epi illuminator has two filter sliders. You can use suitable ND filters by operating the filter sliders. ND filters are used to adjust the light intensity. A large number filter has a low transmittance and is used to get a dark image. -
Page 35: Optical Path Selection (For Trinocular Eyepiece Tube)
Chapter 3 Individual Operations 3.3 Optical Path Selection (for Trinocular Eyepiece Tube) Optical Path Selection (for Trinocular Eyepiece Tube) Light Distribution 3.3.1 With the trinocular eyepiece tube, the optical path Optical path selection lever selection lever allows the light distribution to the binocular section and vertical tube section. -
Page 36: Stage Vertical Movement (Focusing)
Chapter 3 Individual Operations 3.4 Stage Vertical Movement (Focusing) Stage Vertical Movement (Focusing) Prohibited Actions 3.4.1 Avoid the following actions, which can cause equipment malfunctions. Never do them at all. Rotating the right and left coarse/fine focus knobs in opposite directions. •... -
Page 37: Adjusting The Rotating Torque Of The Coarse Focus Knob
Chapter 3 Individual Operations 3.4 Stage Vertical Movement (Focusing) Adjusting the Rotating Torque of the Coarse Focus Knob 3.4.4 Adjust the rotation torque of the coarse focus ring (rotation resistance) by turning the torque adjustment ring (TORQUE) located at the base of the coarse focus knob. -
Page 38: Stage Rotation
Chapter 3 Individual Operations 3.5 Stage Rotation Stage Rotation Stage Rotation 3.5.1 To rotate the circular graduated stage, loosen the stage rotation clamp screw and turn the whole Vernier stage carefully by hand. The angle of rotation can be read to 0.1 degrees with the two vernier scales. -
Page 39: Diopter Adjustment
Chapter 3 Individual Operations 3.6 Diopter Adjustment Diopter Adjustment Diopter adjustment compensates for differences in visual acuity between the right and left eyes, improving binocular observation. It also minimizes focal deviations when switching objectives. In the case of a polarizing microscope, since an eyepiece containing crosshairs is used for the right eye, the procedure for adjusting the diopter differs from that of an ordinary microscope. -
Page 40: Adjusting The Condenser Position
Chapter 3 Individual Operations 3.8 Adjusting the Condenser Position Adjusting the Condenser Position Adjust the condenser position (focusing and centering) so that the light passing through the condenser forms an image at the correct location (center of the optical path) on the specimen surface. -
Page 41: Adjusting The Aperture Diaphragm
Chapter 3 Individual Operations 3.9 Adjusting the Aperture Diaphragm Adjusting the Aperture Diaphragm The aperture diaphragm for the diascopic microscopy is adjusted with the condenser aperture diaphragm knob. For the diaphragms of the epi illuminator, refer to “3.17 Episcopic microscopy.” For the Bright-field Microscopy and the Orthoscopic Microscopy 3.9.1 The numerical aperture for the condenser can be read with the scale on it. -
Page 42: For Conoscopic Microscopy
Chapter 3 Individual Operations 3.10 Selecting a Condenser For Conoscopic Microscopy 3.9.2 For the conoscopic microscopy, the condenser aperture diaphragm functions as a field diaphragm on the conoscopic image surface. Stop down the diaphragm until it circumscribes the circumference of the view field of the conoscopic image (pupil of the objective). 3.10 Selecting a Condenser To perform the polarization microscopy, the P swing-out condenser must be used. -
Page 43: Adjusting The Field Diaphragm
Chapter 3 Individual Operations 3.11 Adjusting the Field Diaphragm Adjusting the Field Diaphragm 3.11 The field diaphragm restricts illumination to the area of the specimen that is being viewed. Operating the field diaphragm control changes the size of the field diaphragm. For normal observation, the size of the diaphragm should be such that it is just outside the edge of the viewfield. -
Page 44: Centering The Objective
Chapter 3 Individual Operations 3.13 Centering the Objective Centering the Objective 3.13 To perform the polarization microscopy, the center of the objective optical path must be aligned to the rotation center of the circular graduated stage. This product comes with the centering nosepiece. -
Page 45: Oil Immersion Operation
Chapter 3 Individual Operations 3.14 Oil Immersion Operation Oil Immersion Operation 3.14 Objectives marked “oil” are oil-immersion type objectives. These objectives are used with immersion oil (option) between the specimen and the tip of the objective. Bubbles in the oil will adversely affect the viewing of the image. -
Page 46: Water Immersion Operation
Chapter 3 Individual Operations 3.15 Water Immersion Operation Water Immersion Operation 3.15 Objectives marked “WI” or “W” are water-immersion type objectives. These objectives are used with immersion water (distilled water or physiological saline) applied between the specimen and the tip of the objective. Microscopy procedures are the same as for oil-immersion type objectives. -
Page 47: Polarization Microscopy
Chapter 3 Individual Operations 3.16 Polarization Microscopy Polarization Microscopy 3.16 Operation of the Polarizers 3.16.1 Polarizer for the diascopic microscopy To perform the diascopic polarization microscopy, attach the polarizer for the diascopic microscopy at the bottom of the condenser. The orientation indicator (a circle mark on the outer Dia polarizer frame of the polarizer) must be facing toward the operator. -
Page 48: Operation Of The Analyzer
Chapter 3 Individual Operations 3.16 Polarization Microscopy Operation of the Analyzer 3.16.2 Attach/detach the analyzer The intermediate tube has the analyzer slider. The analyzer can be placed into the optical path with Analyzer the operation of the slider. slider To place the analyzer into the optical path, pull out the slider. -
Page 49: Azimuth Adjustment Of The Polarizer And Analyzer
Chapter 3 Individual Operations 3.16 Polarization Microscopy Azimuth adjustment of the polarizer and analyzer 3.16.3 For the diascopic observation (1) Push in the analyzer setting knob to move the analyzer out of the optical path. (2) Focus on the specimen. (3) Pull out the analyzer setting knob to move the Dia polarizer analyzer into the optical path. -
Page 50: Bertrand Lens Operation
Chapter 3 Individual Operations 3.16 Polarization Microscopy Bertrand Lens Operation 3.16.4 The intermediate tube has the Bertrand lens. The Bertrand lens can be placed into the optical path to perform the conoscope observation. Setting the Bertrand lens Put the Bertrand lens turret in the “B” position to move the Bertrand lens into the optical path. -
Page 51: P-Cl 1/4Λ & Tint Plate
Chapter 3 Individual Operations 3.16 Polarization Microscopy P-CL 1/4λ & Tint Plate 3.16.5 The intermediate tube has a slot for the P-CL 1/4λ & tint plate. It is used not only for the plate but also for the optional P-CS Senarmont compensator or P-CQ quartz wedge to perform the retardation measurement. - Page 52 Chapter 3 Individual Operations 3.16 Polarization Microscopy P-CQ quartz wedge The P-CQ quartz wedge is used by inserting it into the slot of the intermediate tube in place of the P-CL 1/4λ & tint plate. The quartz wedge is engraved with a scale and can be used for rough measurement of retardation in P-CQ quarts wedge the range of 1λ...
-
Page 53: Episcopic Microscopy
Chapter 3 Individual Operations 3.17 Episcopic Microscopy Episcopic Microscopy 3.17 To perform episcopic microscopy, attach the LV-UEPI universal epi illuminator to the microscope. Switching the Episcopic Illumination 3.17.1 You can switch the illumination between the bright-field and the dark-field to be used for the episcopic microscopy by operating the illumination selection lever on the right side of the epi illuminator. -
Page 54: Aperture Diaphragm In The Epi Illuminator
Chapter 3 Individual Operations 3.17 Episcopic Microscopy Centering the field diaphragm (1) Perform steps in “2.4 Episcopic Microscopy (with the Epi Illuminator Option)” and focus on the specimen using the 10X objective under the episcopic illumination. (2) Lower the field diaphragm open/close lever to stop down the field diaphragm. (3) Turn the field diaphragm centering screws (on both sides) so that the field diaphragm image is positioned in the center of the view field. -
Page 55: Operating Filter On The Epi Illuminator
Chapter 3 Individual Operations 3.17 Episcopic Microscopy Operating Filter on the Epi Illuminator 3.17.4 The LV-UEPI universal epi illuminator has two filter sliders. You can use suitable filters by operating the filter sliders. Filter sliders Filters Usage NCB11 (color balancing filter) For color balance adjustment and color photomicrography ND4 (ND filter) Brightness adjustment (transmittance 25%) -
Page 56: Image Capturing
Chapter 3 Individual Operations 3.18 Image Capturing Image Capturing 3.18 Microscopy images can be captured by attaching a camera head to the trinocular eyepiece tube. For detailed information, refer to the operating manual provided with the camera head or camera control software. Proper adjustment of light intensity and focus on the microscope are important for obtaining clear images. -
Page 57: Making Adjustments To Keep Out Ambient Light
Chapter 3 Individual Operations 3.18 Image Capturing Making Adjustments to Keep out Ambient Light 3.18.5 • Field diaphragm: Stop down the diaphragm to a setting just slightly wider than the area shown on the monitor. • Eyepiece: Cover the eyepiece with a piece of cloth. Anti-vibration Measures 3.18.6 If the exposure is less than 1/8 of a second, reduce light intensity with ND filters to make... -
Page 58: Assembly
Assembly System Configuration TV vertical TV vertical tube adapter tube adapter 0.55X Eyepieces Eyepieces Trinocular Binocular eyepiece tube eyepiece tube P-CL 1/4λ & tint plate P-CS Senarmont compensator Polarizing intermediate tube P-CQ quartz wedge (with analyzer slider) Polarizer slider Filter slider (x2) Quintuple Filters DIN slot... -
Page 59: Assembly Procedure
If the indicated voltage is different, do not indication use the microscope. Contact your nearest Nikon representative. Attaching the circular graduated stage (1) Remove the cushioning material from the substage Specimen holder section and turn the coarse focus handle until the... -
Page 60: Attaching The Nosepiece
Chapter 4 Assembly Attaching the nosepiece Attach the P-N quintuple centering nosepiece on this microscope. Clamp screw for the nosepiece (1) Lift the nosepiece from a position just forward of the point directly below the fitting part and slide toward the back to attach. Continue sliding the nosepiece until its front position is aligned with that of the fitting part. - Page 61 (for at least 30 minutes after the lamp is turned off), before replacing the lamp. • Use the Nikon LV-LH50PC halogen lamphouse for the lamphouse. (1) Loosen the clamp screw on the top side of the Mount the lamphouse...
-
Page 62: Attaching The Polarizing Intermediate Tube
Chapter 4 Assembly Attaching the polarizing intermediate tube (1) Loosen sufficiently the clamp screw for the intermediate tube (or the illuminator) on the microscope arm. Use the tool provided with the microscope to loosen the clamp screw for the epi illuminator. (2) Fit the circular dovetail of the polarizing intermediate tube into the circular dovetail groove Clamp screw for... - Page 63 Chapter 4 Assembly Attaching a camera (for the trinocular eyepiece tube) To attach a photomicrography device such as a camera onto the vertical tube of the trinocular eyepiece tube, you must use two adapters: First, attach the TV vertical tube adapter or the TV vertical tube adapter 0.55X onto the trinocular eyepiece tube.
-
Page 64: Replacing Consumables
Replacing Consumables Replacing the lamp CAUTION • Be careful to avoid burns: Wait until the lamp and nearby parts have cooled before attempting to replace the lamp. • Be careful to avoid electrical shock: Turn off the power switch and unplug the power cord from the outlet. •... - Page 65 (for at least 30 minutes after the lamp is turned off), before replacing the lamp. • Use the Nikon LV-HL50W 12V 50W LONGLIFE halogen lamp or non-Nikon 12V 50W SHORTLIFE halogen lamp (model OSRAM HLX 64610, OSRAM HLX 64611, or PHILIPS 7027) for the lamp. If you wish to buy these lamps, please contact your nearest Nikon representative.
- Page 66 Chapter 5 Replacing Consumables 5.1 Replacing the lamp (1) Loosen the lamphouse cover clamp screw using the hexagonal wrench. (2) Remove the lamphouse cover. (3) Push down the lamp clamp lever and remove the old lamp. (4) With the lamp clamp lever held down, insert the electrodes of a new lamp into the pin holes of the socket.
-
Page 67: Troubleshooting
Troubleshooting When the product does not function properly, take appropriate action as described below. If the problem is still not resolved after referring to "Troubleshooting," please contact your nearest Nikon representative. Optical System Problem Cause Countermeasure Attach the parts (nosepiece, condenser, Some parts are attached incorrectly. - Page 68 Chapter 6 Troubleshooting 6.1 Optical System Problem Cause Countermeasure The nosepiece is not attached Attach the nosepiece correctly. And rotate correctly. Or its rotation has not it to the click stop position. stopped at the click stop position. The focus is uneven. The specimen is not secured in place Place the specimen properly on the stage.
-
Page 69: Mechanical System
Chapter 6 Troubleshooting 6.2 Mechanical System Mechanical System Problem Possible causes Remedy The image is not in focus although the objective is The stage has been attached Install the stage correctly. raised to the highest incorrectly. position. Set the specimen on the stage with the The specimen is placed upside-down. -
Page 70: Care And Maintenance
Care and Maintenance Lens Cleaning Do not let dust, fingerprint, etc. get on the lenses. Dirt on the lenses, filters, etc. will adversely affect the view of image. If any of the lenses get dirty, clean them as described below. Either brush away dust with a soft brush, or else gently wipe it off with a piece of gauze. -
Page 71: Storage
Before putting on the dust-proof cover, turn off the power switch of the microscope (flip it • to the “O” position) and wait until the lamphouse gets cool sufficiently Periodical Inspection (Fee Charged) Periodical inspections (expenses charged) of this product are recommended in order to maintain peak performance. Contact your nearest Nikon representative for details. -
Page 72: Specifications
Specifications Specifications Nikon microscope ECLIPSE 50i POL Model ECLIPSE 50i POL Optical system Infinity-corrected CF optical system Objective: CFI60 Eyepiece: Field number 22 (for binocular eyepiece tube) Field number 25 (the trinocular eyepiece tube) Nosepiece: quintuple (with centering mechanism for objectives) - Page 73 Chapter 8 Specifications 8.1 Specifications External dimensions and External dimensions: weight (main unit) 269 (W) x 375 (H) x 357 (D) mm (including intermediate tube and stage but excluding protrusions) Weight: Approx. 11 kg • This is UL-listed product. (UL61010A-1) Safety standard •...