Recorded Volumes Fall Within Specified Ranges - Siemens Hematek 3000 System Instruction Manual

8 Troubleshooting
Record volumes
Check rinse volume
Check stain-to-buffer ratio
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8.5.3.1

Recorded volumes fall within specified ranges

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8. Place the free end of the rinse tube into a 25mL graduated cylinder.
NOTICE
If glass slides are not placed correctly in the grooves of the conveyor
spirals, this can cause them to break
Damage to the system can result.
• Glass slides must be inserted into the grooves of the conveyor spirals
so they are parallel to the slide loading lines inscribed on the platen, see
Slide loading lines on page 4-8.
9. Load 10 blank glass slides.
10. Wait until the glass slides have been delivered into the slide drawer.
11. Record the volume of rinse, stain, and buffer pumped into each cylinder.
12. Check if the rinse volume is approximately 10mL for 10 blank glass
slides.
13. Calculate the stain-to-buffer ratio. For an example of the calculation of
the stain-to-buffer ratio, see Chapter 9.2.1.
14. Check if the stain-to-buffer ratio is between 1:2 and 1:3.
Note: If the stain-to-buffer ratio is slightly out of the range of 1:2 to 1:3
but the capillary space between the platen and the glass slides is filling
properly, good mixing is occurring, and the stained glass slides are
acceptable under the microscope, the stain-to-buffer ratio should not be
corrected.
15. Proceed as follows:
• If the rinse volume is approximately 10mL for 10 blank glass slides and
the stain-to-buffer ratio is between 1:2 and 1:3, go to Chapter 8.5.3.1.
• If the rinse volume deviates significantly from 10mL for 10 blank glass
slides and/or the stain-to-buffer ratio is not between 1:2 and 1:3, go to
Chapter 8.5.3.2.
If the rinse volume is approximately 10mL for 10 blank glass slides and the
stain-to-buffer ratio is between 1:2 and 1:3, proceed as follows:
1. Connect the tubes to the spouts under the platen. The tube from the
stain pump must lead to the right spout. The tube from the buffer pump
must lead to the middle spout. The tube from the rinse pump must lead
to the left spout.
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Hematek 3000 System – Instruction Manual – Version 1.3