Replacing The Underplaten Tubing; Removing The Underplaten Tubing - Siemens Hematek 3000 System Instruction Manual

7 Performing maintenance
Insert cannulas into bottles
Vent bottles
7
Prepare staining
7.4

Replacing the underplaten tubing

7.4.1

Removing the underplaten tubing

7 8
14. Make sure that at least 7mm (1/4in) of the tube is connected to the
nipple.
15. Execute step 9 to step 14 for each tube.
16. Left of the pump assemblies, turn the pump tubing release knob to the
lock position, see Chapter 4.1.1.
17. Insert the appropriate cannula into its respective bottle in the Hematek
Stain Pak by puncturing the center of the indentation in the visible corner
of the bottle.
18. Remove the cannula, turn it 1/4 turn, and insert it again into the same
puncture. The double puncture creates a slightly larger hole for venting.
19. Push the cannula down until the guard at the top touches the plastic
container.
20. Decide if you want to have additional venting.
• If so: Go to step 21.
• If not: Go to step 24.
21. Remove the cannula.
22. With the cannula, make a second hole in the top of the bottle, near the
indentation.
23. Set the cannula back in the puncture within the indentation of the bottle.
24. Repeat step 17 to step 23 for each bottle.
25. Close the lid.
26. Prime the pump tubing, see Chapter 5.2.
27. Load four priming slides, see Chapter 5.3.
28. Check if the capillary gap between the priming slides and the platen is
properly filled with stain, buffer, and rinse during the staining of the
priming slides. Little patches in the corners could remain dry.
• If so: You can now load smeared glass slides, see Chapter 5.4.
• If not: Go to Chapter 8.5.1.
The underplaten tubing is the tubing between the spouts under the platen
and the nipples on the front side of the middle panel.
To remove the underplaten tubing, proceed as follows:
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Hematek 3000 System – Instruction Manual – Version 1.3