Epi-Fluorescence Attachment; P2-Efli Epi Fluorescence Attachment - Nikon SMZ25 Instruction Manual

9

Epi-Fluorescence Attachment

The microscope system can be used with the status detection model P2-EFLI Epi Fluorescence Attachment and Motorized
P2-EFLM Epi Fluorescence Attachment.

P2-EFLI Epi Fluorescence Attachment

9.1
This illuminator is attached between the zooming body and the
tube. A HG precentered fiber illuminator is used as the light
source. The light coming from the light source is led into the
device by the HG fiber and then passed through the filter cube
attached to the internal turret for epi-fl illumination.
The turret can be attached with up to four filter cubes; the
addresses of the attachment positions are FL1 to FL4. For the
attachable filter cube types, those for fluorescence, bright-field,
and empty are available. Turn the filter cube switching knob to
assign to the indicator the turret address (FL1 to FL4) of the filter
cube to be placed into the optical path.
For systems where a control box has been connected, the
information about the turret address on the optical path is sent to
the control box via a motorized focus unit or relay box, and then
transmitted to the DS-L4 camera control unit and personal
computer.
CAUTION
• Do not use the epi-fluorescence attachment when the filter cube replacement cover is open. Harmful light including ultraviolet
light may leak and affect fluorescence observation.
• Immediately after diascopic observation using an epi-fluorescence attachment, the front face of the empty cube may become
hot, resulting in burns. Do not touch the front face of the empty cube. Replace the empty cube several minutes after finishing
the diascopic observation.
Be sure to attach four cubes to the turret.
Attach an empty cube to the position to which no filter cube is attached.
 Filter cube
• P2-EFL GFP-B, P2-EFL GFP-L, and P2-EFL RFP Filter Cubes: Used for fluorescence observation with a given
wavelength.
• P2-EFLC Filter Cube: Used for fluorescence observation with an arbitrary wavelength when combined with arbitrary
filters, for example.
• P2-EFLBFA Filter Cube (Bright Field): Used for bright-field episcopic observation.
• Empty cube (Three cubes are supplied with an epi-fluorescence attachment): Used when performing diascopic
illumination observation using the epi-fluorescence attachment.
Light shielding plate
The device is equipped with a light shielding plate for preventing the reflection of ultraviolet light. Use the light shielding
plate when performing fluorescence microscopy. When in use, the light shielding plate can be pushed aside to positions
up to 45° horizontally to the rear. When not in use, the plate can be pushed aside to positions up to 170°.
Chapter 8
Functions and Operations of the Devices
Index mark
Turret address
indication
P2-EFLI Epi Fluorescence Attachment
118
BIN O
PH OT O
Filter cube indication
label
(label attachment
position)
Filter cube
replacement hole
cover
Filter cube
switching knob