Procedure For Dilution Factor: 50 - Hanna Instruments HI83325 Instruction Manual

8.5. PROCEDURE FOR DILUTION FACTOR: 50

Note: For a more accurate dilution, use laboratory-grade glass
pipettes and volumetric flasks.
• Add 10 mL of sample to the graduated cylinder using the 5 mL syringe
(twice).
Note: To measure exactly 5 mL of sample with the syringe, push the
plunger completely into the syringe and insert the tip into the sample.
Pull the plunger out until the lower edge of the seal is on the 5 mL
mark of the syringe.
• Remove the cap and fill the Demineralizer
Bottle with tap water.
• Replace the cap and shake gently for at least
2 minutes.
• Open the upper part of the Demineralizer
Bottle cap and squirt gently the demineralized
water into the cylinder, up to the 100 mL
mark.
• Pour the solution in the large 170 mL beaker,
replace the cap and invert several times to mix.
• Clean and dry the graduated cylinder, then pour 20 mL of the diluted solution from the 170-mL
beaker to the graduated cylinder.
5 mL × 2
25