Turbidity and free/total chlorine meter (44 pages)
Summary of Contents for Hanna Instruments HI83325
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HI83325 Multiparameter Photometer for Nutrient Analysis...
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If you need additional technical information, do not hesitate to e‑mail us at tech@hannainst.com or view our worldwide contact list at www.hannainst.com. All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.
1. PRELIMINARY EXAMINATION Remove the instrument and accessories from the packaging and examine it carefully. For further assistance, please contact your local Hanna Instruments Office or email us at tech@hannainst.com. Each HI83325 is delivered in a rugged carrying case and is supplied with: •...
4. ABBREVIATIONS Absorbance Good Laboratory Practice NIST National Institute of Standards and Technology US Environmental Protection Agency grams per liter (ppt) µg/L micrograms per liter (ppb) mg/L milligrams per liter (ppm) milliliter High Range Low Range Medium Range...
With the CAL Check™ feature users are able to validate the performance of the instrument and apply a user calibration (if necessary). Hanna Instruments CAL Check™ cuvettes are made with NIST traceable standards. Probe mode uses a digital pH probe with a one or two-point calibration.
5.3. FUNCTIONAL DESCRIPTION 1. Splash-proof keypad 2. Liquid Crystal Display (LCD) 3. Indexing mark 4. Protective port covers 5. Light-blocking cover panel 6. Cuvette holder 7. ON/OFF power button 8. 3.5-mm TRRS (jack) input for digital electrodes 9. Standard USB host connector for data transfer to a USB flash drive 10.
Keypad Description The keypad contains 12 direct keys and 3 functional keys with the following functions: Press the functional key to perform the function displayed above it on the LCD. Press to access the list of photometer methods. Press to move up in a menu or a help screen, to increment a set value or to access second level functions.
5.5. OPTICAL SYSTEM Instrument Block Diagram The internal reference system (reference detector) of the HI83325 photometer compensates for any drifts due to power fluctuations or ambient temperature changes, providing a stable source of light for your blank (zero) measurement and sample measurement.
Zero measurement). If a photometer measurement is on the screen, an auto-log is created before shutdown. 6.2. MODE SELECTION HI83325 has two operational modes: Photometer and Probe. Photometer mode enables on-demand measurement of a cuvette using the integrated optical system.
6.3. GENERAL SETUP Press the SETUP key to enter in Setup menu, highlight desired option using the keys and press Select. CAL Check (Photometer Mode Only) Press Select to enter the CAL Check screen. The date, time and values for the last CAL Check are displayed on the screen.
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Date & Time Press Modify to change the date and time. Press the functional keys or the keys to highlight the value to be modified (year, month, day, hour, minute or second). Use the keys to change the value.
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Language Option: Português, Deutsch, English, Español, Français, Italiano, Dutch Press Modify to change the language. Use the keys to select the desired language. Press Select to change the language. Beeper Option: Enable or Disable When enabled, a short beep is heard every time a key is pressed. A long beep alert sounds when the pressed key is not active or an error is detected.
ESC key to return to the Setup menu. 6.4. CONTEXTUAL HELP HI83325 offers an interactive contextual help mode that assists the user at any time. To access the help screen press the HELP key. The instrument will display additional information related to the current screen.
7. LOGGING DATA & DATA MANAGEMENT The instrument features a data log function to help you keep track of all your analysis. The data log can hold 1000 individual measurements. Storing, viewing and deleting the data is possible using the LOG and RECALL keys.
Explorer or Mac Finder) to move the file from the meter to the PC. Log data is exported as a single file (HI83325.csv) containing all logged photometer and probe data. The CSV file may be opened with a text editor or spreadsheet application.
8. NUTRIENT SAMPLES PREPARATION GUIDE 8.1. INTRODUCTION TO PLANT NUTRIENTS The three elements that are mostly needed by the plants are nitrogen (N), phosphorus (P) and potassium (K). They are called the macronutrients while other elements, needed by plants in smaller amounts, are called microelements.
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Phosphorus Phosphorus (P) has an important role in many fundamental biochemical and physiological processes. Plants take up phosphorus in the form of phosphate ion (PO PRESENT IN DNA and RNA, ATP, ADP • stimulates the roots growth • stimulates blooming ACTION •...
Irrigation Water In agricultural areas it is quite common to find altered values in the chemical composition of irrigation waters. The problem concerns mostly the high nitrate concentration, usually determined by excessive fertilization or irrational liquid manure spreading. The analysis of irrigation waters allows us to find out which are the substances present in major or minor quantity and to organize an advantageous fertilization plan.
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Recommended Procedures/Dilutions According to Nutrient Concentration: Dilution Method Parameter Estimated Concentration Typical Usage Factor Selection Ammonia < 2.5 ppm NH No dilution Ammonia LR Irrigation Water 2.5 - 9 ppm NH No dilution Ammonia MR Irrigation Water 9 - 100 ppm NH No dilution Ammonia HR Recycled Nutrient Solution...
8.3. PROCEDURE FOR DILUTION FACTOR: 5 Note: For a more accurate dilution, use laboratory-grade glass pipettes and volumetric flasks. • Use the graduate cylinder to measure exactly 20 mL of sample. • Remove the cap and fill the Demineralizer Bottle with tap water. •...
8.4. PROCEDURE FOR DILUTION FACTOR: 10 Note: For a more accurate dilution, use laboratory-grade glass 5 mL × 2 pipettes and volumetric flasks. • Add 10 mL of sample to the graduated cylinder using the 5 mL syringe (twice). Note: To measure exactly 5 mL of sample with the syringe, push the plunger completely into the syringe and insert the tip into the sample.
8.5. PROCEDURE FOR DILUTION FACTOR: 50 Note: For a more accurate dilution, use laboratory-grade glass pipettes and volumetric flasks. 5 mL × 2 • Add 10 mL of sample to the graduated cylinder using the 5 mL syringe (twice). Note: To measure exactly 5 mL of sample with the syringe, push the plunger completely into the syringe and insert the tip into the sample.
• Open the upper part of the Demineralizer Bottle cap and squirt gently the demineralized water into the cylinder, up to the 100 mL mark. • Clean and dry the large 170 mL beaker, then pour the solution from the graduated cylinder to the large 170 mL beaker, replace the cap and invert several times to mix.
9. PHOTOMETER MODE 9.1. METHOD SELECTION In order to select the desired method press the METHOD key and a screen with the available methods will appear. Press the keys to highlight the desired method. Press Select. After the desired method is selected, follow the procedure described in the related section. Before performing a method, read all the instructions carefully.
Proper Use of Dropper Bottle 1. Tap the dropper on the table several times. 2. Remove the cap and wipe the outside of the tip with a cloth. 3. Keep the dropper bottle in a vertical position while dosing the reagent. Proper Use of Powder Packet 1.
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(b) Shaking the cuvette, moving the cuvette up and down. The movement may be gentle or vigorous. This mixing technique is indicated with “shake gently” or “shake vigorously”, and one of the following icons: shake gently shake vigorously (c) Swirl the cuvette gently to mix the solution. This mixing technique is indicated with one of the following icons: In order to avoid reagent leaking and to obtain more accurate measurements, close the cuvette first with the supplied High-Density Polyethylene (HDPE)
Discard the sample immediately after the reading is taken, or the glass might become permanently stained. All the reaction times reported in this manual are at 25 °C (77 °F). In general, the reaction time should be increased for temperatures lower than 20 °C (68 °F) and decreased for temperatures higher than 25 °C (77 °F).
9.6. METER VALIDATION & CAL CHECK ® Warning: Do not validate the meter with standard solutions other than the Hanna Instruments CAL Check Standards. For accurate validation results, please perform tests at room temperature, 18 to 25 ° C (64.4 to 77.0 ° F).
4. Insert a cuvette filled with deionized water, then press Zero. 5. Insert the prepared sample cuvette, then press Read. ® Warning: Never use absorbance methods for validation using Hanna Instruments CAL Check cuvettes. The factory calibration corrections for CAL Check cuvettes are applied while in CAL Check...
® HI83325 can be used to perform direct pH measurements by connecting a Hanna Instruments digital pH electrode with a 3.5 mm TRRS connector. Connect the electrode to the 3.5 mm port marked with EXT PROBE located at the rear of the meter. If the meter is in Photometer mode, set the meter to Probe mode by pressing the MODE key.
10.2. pH CALIBRATION From the probe measurement screen, press Calibrate to begin the calibration process. During pH calibration, the display will show the current pH reading, temperature reading, selected buffer type and the buffer number (“Buffer: 1” for the first buffer, “Buffer: 2” for the second buffer). Press Clear to clear the current calibration.
10.3. pH MESSAGES & WARNINGS No Probe No probe is connected or the probe is broken. Connecting The meter has detected a probe and is reading the probe configuration and calibration information. Incompatible Probe The connected probe is not compatible with this device. Incompatible Calibration The probe’s current calibration is not compatible with this meter.
Check Probe & Buffer There is a large difference between the pH measurement and the selected buffer value or the electrode slope is outside of the accepted slope limit. Clean the probe and confirm the correct buffer selection. Wrong Temperature The buffer temperature is outside of the acceptable window for the selected buffer value.
10.5. pH ELECTRODE CONDITIONING & MAINTENANCE Remove the protective cap. Do not be alarmed if salt deposits are present, this is normal. Rinse the probe with water. Shake the electrode down as you would do with a clinical thermometer to eliminate any air bubbles inside the glass bulb.
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Periodic Maintenance Inspect the electrode and the cable. The cable used for connection to the instrument must be intact and there must be no points of broken insulation on the cable, connectors must be perfectly clean and dry. If there are any scratches or cracks on the electrode stem or bulb, replace the electrode. For refillable electrodes, refill the reference chamber with fresh electrolyte (HI7082 HI8082...
11. METHOD PROCEDURES 11.1. AMMONIA LOW RANGE SPECIFICATIONS Range 0.00 to 3.00 mg/L (as NH Resolution 0.01 mg/L Accuracy ±0.04 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1426 Nessler Method REQUIRED REAGENTS Code...
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• Remove the cuvette. • Add 4 drops of HI93700A-0 Ammonia Low Range Reagent ×4 A. Replace the plastic stopper and the cap. Swirl to mix the solution. • Add 4 drops of HI93700B-0 Ammonia Low Range Reagent ×4 B. Replace the plastic stopper and the cap. Swirl to mix the solution.
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• Press Chem Frm to convert the result to mg/L of ammonia (NH ) and ammonium (NH • Press the or key to return to the measurement screen. INTERFERENCES Interference may be caused by: • Hardness above 1 g/L •...
11.2. AMMONIA MEDIUM RANGE SPECIFICATIONS Range 0.00 to 10.00 mg/L (as NH Resolution 0.01 mg/L Accuracy ±0.05 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1426, Nessler Method REQUIRED REAGENTS Code...
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• Remove the cuvette. ×4 • Add 4 drops of HI93715A-0 Ammonia Medium Range Reagent A. Replace the plastic stopper and the cap. Swirl to mix the solution. • Add 4 drops of HI93715B-0 Ammonia Medium Range Reagent B. ×4 Replace the plastic stopper and the cap.
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• Press Chem Frm to convert the result to mg/L of ammonia (NH ) and ammonium (NH • Press the or key to return to the measurement screen. INTERFERENCES Interference may be caused by: • Hardness above 1 g/L •...
11.3. AMMONIA HIGH RANGE SPECIFICATIONS Range 0.0 to 100.0 mg/L (as NH Resolution 0.1 mg/L Accuracy ±0.5 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 420 nm Method Adaptation of the ASTM Manual of Water and Environmental Technology, D1426, Nessler Method REQUIRED REAGENTS Code...
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• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Remove the cuvette. • Add 4 drops of HI93733A-0 Ammonia High Range Reagent A. ×4 Replace the plastic stopper and the cap. Swirl to mix the solution. •...
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• Press Chem Frm to convert the result to mg/L of ammonia (NH ) and ammonium (NH • Press the or key to return to the measurement screen. INTERFERENCES Interference may be caused by: • Hardness above 1 g/L •...
11.4. CALCIUM SPECIFICATIONS Range 0 to 400 mg/L (as Ca Resolution 1 mg/L Accuracy ±10 mg/L ±5% of reading at 25 °C Light Source LED with narrow band interference filter @ 466 nm Method Adaptation of the Oxalate Method REQUIRED REAGENTS Code Description Quantity...
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• Replace the plastic stopper and the cap. Invert several times to mix. • Insert the cuvette into the holder and close the lid. • Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement. •...
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• After waiting 5 minutes, invert the cuvette 10 times to mix (about 15 seconds). • Insert the cuvette into the holder and close the lid. • Press Read to start the reading. The instrument displays the results in mg/L of calcium (Ca INTERFERENCES Interference may be caused by: •...
11.5. IRON(II)/(III) SPECIFICATIONS Range 0.00 to 6.00 mg/L (as Fe) Resolution 0.01 mg/L Accuracy ±0.10 mg/L ±2% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of Standard Methods for the Examination of Water and Wastewater, 23 Edition, 3500-Fe B, Phenanthroline Method REQUIRED REAGENTS...
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MEASUREMENT PROCEDURE • Select the Iron(II)/(III) method using the procedure described in the METHOD SELECTION section. • Fill the cuvette with 10 mL of unreacted sample (up to the mark). 10 mL Replace the plastic stopper and the cap. • Insert the cuvette into the holder and close the lid. •...
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• Press Timer and the display will show the countdown prior to the measurement or wait 3 minutes and press Read 1. The instrument displays the result in mg/L of Iron (Fe Warning: Timing is critical for accurate measurement. Reaction times beyond 3 minutes may cause some ferric iron (Fe ) o also react, producing false high measurements.
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• Press Chem Frm to cycle through the available chemical forms of Fe and Fe Note: Each chemical form can be logged independently by pressing the LOG key. • Press the or key to return to the measurement screen. INTERFERENCES Interference may be caused by: •...
11.6. MAGNESIUM SPECIFICATIONS Range 0 to 150 mg/L (as Mg Resolution 1 mg/L Accuracy ±5 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 466 nm Method Adaptation of the Calmagite Method REQUIRED REAGENTS Code Description Quantity...
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• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement. 0.5 mL of sample • Remove the cuvette. • Add 0.5 mL of sample to the cuvette using the second 1 mL syringe. •...
11.7. NITRATE SPECIFICATIONS Range 0.0 to 30.0 mg/L (as NO - N) Resolution 0.1 mg/L Accuracy ±0.5 mg/L ±10% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of the Cadmium Reduction Method REQUIRED REAGENTS Code Description...
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• Remove the cuvette and add one packet of HI93728-0 Nitrate Reagent. • Replace the plastic stopper and the cap. Shake vigorously up and down for exactly 10 seconds. Continue to mix by inverting the cuvette gently for 50 seconds, while taking care not to induce air bubbles.
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• Press Chem Frm to convert the result to mg/L of nitrate (NO ¯). • Press the or key to return to the measurement screen. INTERFERENCES Interference may be caused by: • Ammonia and amines, as urea and primary aliphatic amines •...
11.8. PHOSPHATE HIGH RANGE SPECIFICATIONS Range 0.0 to 30.0 mg/L (as PO Resolution 0.1 mg/L Accuracy ±1.0 mg/L ±4% of reading at 25 °C Light Source LED with narrow band interference filter @ 525 nm Method Adaptation of Standard Methods for the Examination of Water and Wastewater, 18 Edition, Amino Acid Method REQUIRED REAGENTS...
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• Add 10 drops of HI93717A-0 Phosphate HR Reagent A. ×10 • Add one packet of HI93717B-0 Phosphate HR Reagent B to the cuvette. Replace the plastic stopper and the cap. Shake gently until completely dissolved. • Insert the cuvette into the holder and close the lid. •...
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• Press the or key to return to the measurement screen. INTERFERENCES Interference may be caused by: • Sulfide • Chloride above 150000 mg/L • Magnesium above 40000 mg/L CaCO • Calcium above 10000 mg/L CaCO • Iron (Ferrous) above 100 mg/L...
11.9. POTASSIUM SPECIFICATIONS Range 0.0 to 20.0 mg/L (as K) Resolution 0.1 mg/L Accuracy ±3.0 mg/L ±7% of reading at 25 °C Light Source LED with narrow band interference filter @ 466 nm Method Adaptation of the Turbidimetric Tetraphenylborate Method REQUIRED REAGENTS Code Description...
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• Press Zero. The display will show “-0.0-” when the meter is zeroed and ready for measurement. • Add one packet of HI93750B-0 Potassium Reagent B. Replace the plastic stopper and the cap. Shake gently for 1 minute. • Insert the cuvette into the holder and close the lid. •...
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• Press Chem Frm to convert the result to mg/L of potassium oxide (K • Press the or key to return to the measurement screen. INTERFERENCES Interference may be caused by: • Chloride above 12000 mg/L • Calcium above 10000 mg/L CaCO •...
11.10. SULFATE SPECIFICATIONS Range 0 to 150 mg/L (as SO Resolution 1 mg/L Accuracy ±5 mg/L ±3% of reading at 25 °C Light Source LED with narrow band interference filter @ 466 nm Method Sulfate is precipitated with barium chloride crystals REQUIRED REAGENTS Code Description...
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• Add one packet of HI93751-0 Sulfate Reagent. • Replace the plastic stopper and the cap. Invert gently for 1 minute (about 30 inversions). • Insert the cuvette into the holder and close the lid. • Press Timer and the display will show the countdown prior to the measurement or wait 5 minutes and press Read.
There is an excess amount of ambient light reaching the detector. Make sure the lid is closed before performing any measurements. If the issue persists, please contact Hanna Instruments technical support. The sample and the zero cuvettes are inverted. Swap the cuvettes and repeat the measurement.
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The measured value cannot be calculated. Please check sample preparation and measurement procedure. Stored results of the CAL Check measurements have been lost. Please redo the CAL Check measurements to ensure accurate results. User settings have been lost. Please reset the values. If the issue persists, please contact Hanna Instruments technical support.
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Real time clock is not accurate. Some features are no longer available. Restart the meter. If the issue persists, please contact Hanna Instruments technical support. The device serial number can not be identified. Some features are no longer available. Restart the meter. If the issue persists, please contact Hanna Instruments technical support.
14.3. pH SOLUTIONS BUFFER SOLUTIONS Code Description HI70004P pH 4.01 buffer sachet, 20 mL (25 pcs.) HI70007P pH 7.01 buffer sachet, 20 mL (25 pcs.) pH 10.01 buffer sachet, 20 mL (25 pcs.) HI70010P HI7001L pH 1.68 buffer solution, 500 mL HI7004L pH 4.01 buffer solution, 500 mL HI7006L...
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ELECTRODE CLEANING SOLUTIONS Code Description HI70000P Electrode rinse sachet, 20 mL (25 pcs.) HI7061L General cleaning solution, 500 mL HI7073L Protein cleaning solution, 500 mL HI7074L Inorganic cleaning solution, 500 mL HI7077L Oil & fat cleaning solution, 500 mL HI8061L General cleaning solution in FDA approved bottle, 500 mL HI8073L Protein cleaning solution in FDA approved bottle, 500 mL...
14.4. OTHER ACCESSORIES Code Description HI72083300 carrying case HI731318 cloth for wiping cuvettes (4 pcs.) HI731331 glass cuvette (4 pcs.) HI731335N cap for cuvette (4 pcs.) HI731340 200 µL automatic pipette HI731341 1000 µL automatic pipette HI731342 2000 µL automatic pipette HI740034P cap for 100 mL beaker (10 pcs.) HI740036P...
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USB power adapter, European plug HI75110/220U USB power adapter, USA plug HI76404A electrode holder HI83325-11 CAL Check cuvette kit for HI83325 Sample preparation kit consisting of activated carbon for 50 tests, demineralizer bottle for 10 L of water, 100 mL graduated beaker with cap,...
Damage due to accidents, misuse, tampering or lack of prescribed maintenance is not covered. If service is required, contact your local Hanna Instruments Office. If under warranty, report the model number, date of purchase, serial number (engraved on the bottom of the meter) and the nature of the problem.
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Hanna Instruments reserves the right to modify the design, construction or appearance of its products without advance notice.
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World Headquarters Hanna Instruments Inc. Highland Industrial Park 584 Park East Drive Woonsocket, RI 02895 USA www.hannainst.com MAN83325 Printed in ROMANIA...