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Example: Defining the Sample Volume
If you have programmed the sampler to USE DEFINED
SAMPLE (section 3.5.10), follow the steps in this
example to define the volume using pump counts.
1. At the Main Menu display, press the Calibrate
button.
PROGRAM
VIEW LOG
2. The sampler displays the Calibrate Volume or
Define Sample option screen. Use the Next or
Previous buttons to select DEFINE SAMPLE. Then
press the Enter button.
CALIBRATE VOLUME
DEFINE SAMPLE
3. The sampler displays the pre-purge screen to
define number of counts needed to purge the
suction line. To simply review the pump count
values, press the Next button to advance through
the screens. To change the pump counts, use the
Number buttons to type the pre-purge counts. Or,
press and hold the "1" button to reset the count to
zero and start the pump in reverse to purge the
line. Hold the 1 button until the line is sufficiently
purged.
___ COUNT PRE-PURGE
HOLD '1' TO PURGE
When you release the 1 button the sampler reports
the number of purge counts that elapsed while you
held down the button. Press the Enter button to
save the value and continue.
4. The sampler displays a screen to define number of
counts needed to deliver the desired volume. Use
the Number buttons to type the sample counts. Or,
press and hold the "3" button to reset the count to
zero and start the pump. The pump will draw the
liquid through the suction line and pump, then
CONFIGURE
Isco 4700 Refrigerated Sampler
deposit it into the bottle. To measure the delivered
volume, you can replace the bottle with a
graduated cylinder for this step.
___ COUNT SAMPLE
HOLD '3' TO PUMP
Release the button when the desired volume has
been collected. Press the Enter button to save the
value and continue.
5. The sampler displays the post-purge screen to
define number of counts needed to purge the
suction line. Use the Number buttons to type the
post-purge counts. Or, press and hold the "1"
button to reset the count to zero and start the pump
in reverse to purge the line. Hold the 1 button until
the line is sufficiently purged.
___ COUNT POSTPURGE
HOLD '1' TO PURGE
When you release the 1 button the sampler reports
the number of purge counts that elapsed while you
held down the button. Press the Enter button to
save the value and finish defining the sample.
___ COUNT POSTPURGE
PROGRAM
VIEW LOG
Repeat these steps as needed until the desired sample
volume is collected.
You may find it easiest to press and hold the 1 and 3
buttons the first time through to roughly define the
sample volume. Then, repeat these steps and enter the
pump count values to finely adjust the sample volume.
You can check the defined sample volume using the
Grab Sample feature (section 4.4) and selecting USE
DEFINED SAMPLE.
Section 3 Programming
CONFIGURE
3-21
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