Fused Silica Capillary Issues; Sampler Issues - Agilent Technologies 1100 Series System Manual

• To achieve the best flow stability, especially in the micro mode, %Ripple must be

Fused Silica Capillary issues

• When you connect a capillary (especially at the column) press it smoothly into the
NOTE
and Fittings," starting on page 45 for information on installing and handling capillaries.
• Be careful when you bend a Fused Silica Capillary. The diameter must not be
• When you replace a part, especially a capillary, clean it with Acetone.
• If a fused silica capillary leaks, do not retighten under flow. Set column flow to zero,
• Avoid the use of alkaline solutions (pH > 8.5) which can attack the fused silica from
• Be careful not to crush capillaries when applying module doors.
• A broken capillary can release silica particles into the system (e.g. cell) causing
• A blocked capillary can often be unblocked by back-flushing the capillary. Acetone

Sampler issues

• For fast gradient use valve to bypass function after the sample is transferred to the
• When doing automated gradient runs, use the fast composition
1100 Series Capillary LC System Manual
within acceptable values, typically no worse than 2%.
fitting to avoid air gaps. Incorrect setting will result in dispersion causing tailing or
footing peaks.
Do not overtighten the fused silica capillaries. see
smaller than 40 mm.
reinsert the capillary, tighten and set new column flow.
the capillaries.
problems in the system down-stream of the break.
is a recommended solvent for capillary back-flushing.
column. This function results in smaller delay times and sharper gradient curves.
change/reconditioning function to equilibrate the system between runs.
31
40 mm
Optimizing Performance 2
Chapter 3, "Capillaries