Zeiss Axiostar plus Operating Manual
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Zeiss Axiostar plus Operating Manual

Transmitted-light microscope
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Zeiss Axiostar plus Operating Manual

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  • Page 2 Artisan Scientific - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan-scientific.com...

  • Page 3: Copyright

    Carl Zeiss Copyright Axiostar plus Knowledge of this manual is required for the operation of the instrument. Therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument.

  • Page 4: Table Of Contents

    Axiostar plus Contents Carl Zeiss CONTENTS Page INTRODUCTION Title Page......................I Copyright ......................II Contents ......................III General Notes on Safety, Instrument Safety and EMC .........VI Notes on Safety: Unpacking, Transport, Storage, Disposal and operation.... VII Notes on Safety: Care and Maintenance, Warranty Notes ......... VIII INSTRUMENT DESCRIPTION ................1-3...
  • Page 5 Carl Zeiss Contents Axiostar plus Attaching condensers..................2-14 2.8.1 Attaching Abbe condenser 0.9/1.25................2-14 2.8.2 Inserting slider for phase contrast or darkfield.............. 2-14 2.8.3 Attaching Abbe condenser with 5-position turret............2-15 2.8.4 Attaching LED illuminator ..................... 2-16 2.8.5 Attaching darkfield condenser ..................2-18 Attaching colored disk carrier, polarizer or wide-angle unit ......2-20...
  • Page 6 3.11.3 Attaching cameras with other filter thread sizes ............3-26 3.11.4 Sony DSC-S70, DC-S75, DSC-S85 on digital camera adapter 44 M52x0.75, 000000-1108-984 ..................3-26 3.12 Axiostar plus - Packing the mount ..............3-27 CARE, MAINTENANCE AND TROUBLE-SHOOTING ........4-2 Care and maintenance of the instrument............4-2 Troubleshooting....................4-3 4.2.1...
  • Page 7 CAUTION! representatives. If the safety notes are not observed, there is a The Axiostar plus microscope is an optical risk that the instrument will be destroyed. precision instrument which may be impaired in its performance damaged handled improperly.
  • Page 8 Please observe the following safety notes when Please observe the following safety notes when unpacking, transporting storing using the Axiostar plus microscope: Axiostar plus microscope: In accordance with standard practice, the – microscope is supplied in a plastic container The microscope can be operated with a line –...

  • Page 9: Notes On Warranty

    Axiostar plus Care and Maintenance Notes on Warranty Please observe the following safety notes when The Axiostar plus microscope, including its caring for and maintaining the Axiostar plus original accessories, may only be used for the microscope: microscope techniques described this manual.
  • Page 10 Instrument description Axiostar plus Contents, List of Illustrations Carl Zeiss INSTRUMENT DESCRIPTION Contents INSTRUMENT DESCRIPTION..................1-3 Name and intended application .................. 1-3 Description and main features ..................1-3 System overview......................1-4 Objectives ........................1-7 Eyepieces ........................1-9 Stage micrometers and eyepiece reticles ..............1-9 Technical data ......................
  • Page 11 Instrument description Carl Zeiss Overall view Axiostar plus Fig. 1-1 Overall view B 40-815 e 12/01 Artisan Scientific - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan-scientific.com...

  • Page 12: Instrument Description

    Axiostar plus Intended application, Main features Carl Zeiss Description and main features INSTRUMENT DESCRIPTION Thanks to its pyramid design, the Axiostar plus Name and intended application is a compact transmitted-light microscope. Manufacturer‘s name: In addition to high-resolution ICS objectives and...

  • Page 13: System Overview

    Instrument description Carl Zeiss System overview Axiostar plus System overview B 40-815 e 12/01 Artisan Scientific - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan-scientific.com...
  • Page 14 Instrument description Axiostar plus System overview Carl Zeiss 40-815 e 12/01 Artisan Scientific - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan-scientific.com...
  • Page 15 Instrument description Carl Zeiss System overview Axiostar plus B 40-815 e 12/01 Artisan Scientific - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan-scientific.com...

  • Page 16: Objectives

    Therefore, so-called "Corr" (Carl Zeiss color code) objectives can be set for different cover slip thickness via a correction ring. For this, a 0.25 = numerical aperture specimen area is searched, and the position of ∞...

  • Page 17: Objectives

    Instrument Description Carl Zeiss Objectives Axiostar plus The following objectives are available for the Axiostar plus microscope: Magnification/ Free working Microscopy Cover slip Objective Numerical distance Cat.No. Technique thickness aperture FAA [mm] D [mm] × Transm.-light brightfield CP-Achromat /0.12 11.2 440920-0000-000 ×...

  • Page 18: Eyepieces

    54° 444054-0000-000 Eyepiece PL 16×/16 Br. foc. We recommend using eyecups for the eyepieces; they can be ordered in the Zeiss catalog under Cat. No. 444801-0000-000. Stage micrometers and eyepiece reticles Measuring and counting using the microscope requires stage micrometers and eyepiece reticles, a small...
  • Page 19 Instrument description Carl Zeiss Eyepieces, Stage micrometers, Eyepiece reticles Axiostar plus Crossline disk / d = 26 mm 474064-0000-000 Crossline micrometer disk 10:100 / d = 474066-9901-000 26 mm gradation length = 10 mm increments = 0.1 mm gradation tolerance ≤ 0.001 mm...

  • Page 20: Technical Data

    Stand with phototube................approx. 200 × 350 × 450 mm Overall height including T2 adapter and CONTAX ARIA SLR camera housing....approx. 570 mm Weight Axiostar plus with binocular tube ..................approx. 6.7 kg Ambient conditions Storage and transport (in packaging): Permissible ambient temperature ...................-40 to +70 °C Permissible relative humidity (without condensation) ............
  • Page 21 Instrument description Carl Zeiss Technical Data Axiostar plus Light sources Halogen lamp......................HAL 6 V, 20 W Adjustment of the light source ............continuous, 1.5 to 6 V DC Color temperature at 6 V ...................2800 K Light flux ........................280 lm Average life .........................1000 h...

  • Page 22: Instrument Description

    Instrument description Axiostar plus Technical Data Carl Zeiss Opto-mechanical data Stand with stage focusing................ with coarse drive (4 mm/U) and fine drive (0.4 mm/U) overall lift 15 mm Objective change...............manually via 4/5-position objective revolver Objectives ................ICS line of objectives with W 0.8 thread Eyepieces ......................
  • Page 23 Instrument description Carl Zeiss Technical Data Axiostar plus Binocular tube 30°/20 maximum field number .......................20 interpupillary distance............can be set between 55 and 75 mm viewing angle........................30° viewing angle with ergonomic base plate....30°, can be adjusted by ±6.5° in steps of 2.2°...

  • Page 24: Start-Up

    Start-Up Axiostar plus Contents Carl Zeiss START-UP Contents START-UP ........................2-3 Unpacking the instrument ...................2-3 Attaching and setting ergonomic base plate ..............2-3 2.2.1 Attaching the ergonomic base plate ................2-4 2.2.2 Adjusting the ergonomic base plate ................2-6 Attaching tubes......................2-8 2.3.1 Attaching binocular tube 45°/20 ICS ................2-8 2.3.2...
  • Page 25 Start-Up Carl Zeiss List of illustrations Axiostar plus List of illustrations Fig. 2-1 Inserting base pods......................2-4 Fig. 2-2 Attaching ergonomic base plate ..................2-5 Fig. 2-3 Adjusting ergonomic base plate ...................2-7 Fig. 2-4 Attaching binocular tube / phototube 45° ..............2-8 Fig. 2-5 Attaching binocular tube / phototube 30°...

  • Page 26: Unpacking The Instrument

    Carl Zeiss START-UP Attaching and setting ergonomic base plate Using the Axiostar plus with ergonomic base Before installing and starting-up the Axiostar plate allows you to set the microscope to your plus, carefully read through and observe the individual ergonomic requirements.

  • Page 27: 2.2.1 Attaching The Ergonomic Base Plate

    Start-Up Carl Zeiss Unpacking, Attaching ergonomic base plate Axiostar plus 2.2.1 Attaching the ergonomic base plate • Remove the tube if one is attached. • Tilt the stand back and set it down. • Remove the four plastic feet (Fig. 2-1/1) from the stand and replace with the four base pods included (Fig.
  • Page 28 Start-Up Axiostar plus Attaching and adjusting ergonomic base plate Carl Zeiss Ergonomic base plate Allen screws M4 x 30 Fig. 2-2 Attaching ergonomic base plate B 40-815 e 12/01 Artisan Scientific - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan-scientific.com...

  • Page 29: 2.2.2 Adjusting The Ergonomic Base Plate

    Start-Up Carl Zeiss Attaching and adjusting ergonomic base plate Axiostar plus 2.2.2 Adjusting the ergonomic base plate Setting viewing angle (microscope slope) Adjusting viewing height The slope of the microscope in relation to the table surface or hand rest can be set according The viewing height of the microscope can be to your needs and application.
  • Page 30 Start-Up Axiostar plus Attaching and adjusting ergonomic base plate Carl Zeiss Top of stand Handle on back of ergonomic base plate Adjustment feet Locking button Fig. 2-3 Adjusting ergonomic base plate B 40-815 e 12/01 Artisan Scientific - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan-scientific.com...

  • Page 31: Attaching Tubes

    Start-up Carl Zeiss Attaching tube Axiostar plus Attaching tubes One knurled screw and one hexagonal screw each are supplied with the mount. Any of these screws can be used to clamp the tube (phototube) to the stand. • Screw the required screw into the threaded hole (2-4/6) in the stand.

  • Page 32: Attaching Binocular Tube 30°/20 Ics

    Start-up Axiostar plus Attaching tube Carl Zeiss 2.3.3 Attaching binocular tube 30°/20 Using the binocular tube/phototube 30° with the ergonomic base plate offers the best ergonomic conditions for microscope use. The adapter for 30° tubes is required to attach the binocular tube 30°...

  • Page 33: Screwing In Objectives

    Start-up Carl Zeiss Screwing in objectives, Inserting eyepieces Axiostar plus Screwing in objectives • Remove dust caps (2-6/2) according to the number of objectives and screw objectives (2-6/3) into nosepiece (2-6/1) clockwise one one, starting with lowest magnification. Place dust caps on the unused objective openings in the nosepiece.

  • Page 34: Inserting Eyepiece Reticles

    (2-8/4) to allow easy replacement. Complete mounts with eyepiece reticles can be obtained directly from Zeiss. When reordering reticles for already available eyepieces 444232-9902-000, you must also order the mount 444232-0206-000. For eyepieces 444132-9901-000, order the mount 444132-0206-000.

  • Page 35: Attaching Folding Eyecups

    Start-Up Carl Zeiss Setting interpupillary distance and viewing height Axiostar plus 2.5.3 Attaching folding eyecups The eyepieces are equipped with rubber rings to protect eyeglasses from scratches. These rings can be replaced with folding eyecups, if desired. • Remove the rubber rings (2-8/2) from the eyepieces and attach the eyecups (2-8/1).

  • Page 36: Setting Mechanical Stage 75 X 30 R/L

    Setting mechanical stage 75 x 30 R/L Carl Zeiss Setting mechanical stage 75 x 30 R/L The Axiostar plus stand is delivered from the factory with right or left (R/L) drive operation. A specimen holder with spring lever is attached on the left of the mechanical stage.

  • Page 37: Attaching Condensers

    Start-Up Carl Zeiss Attaching condensers Axiostar plus Attaching condensers 2.8.1 Attaching Abbe condenser 0.9/1.25 • Unscrew both centering screws (2-12/3) on the condenser carrier (2-12/1) until the Abbe condenser 0.9/1.25 (2-12/7) can be easily inserted into the condenser carrier. •...

  • Page 38: Attaching Abbe Condenser With 5-Position Turret

    Start-Up Axiostar plus Attaching condensers Carl Zeiss 2.8.3 Attaching Abbe condenser with 5-position turret Attachment is made in the same way as the Abbe condenser 0.9/1.25. • Loosen both centering screws (2-14/3) on the condenser carrier (2-14/1). • Press condenser with dovetail against the spring pin (2-14/2) in the condenser carrier.

  • Page 39: 2.8.4 Attaching Led Illuminator

    Start-Up Carl Zeiss Attaching condensers Axiostar plus 2.8.4 Attaching LED illuminator • Insert LED illuminator (2-15/3) in the condenser carrier (2-15/1) in the same way as the Abbe condensers. • Tighten both centering screws (2-15/2) until the LED illuminator lies approximately in the center of the beam path.
  • Page 40 Start-Up Axiostar plus Attaching condensers Carl Zeiss Operation with Alkaline battery (9 V / Ni Cd battery block) • Turn holding screw (2-16/8) on the cover (2-16/7) of the battery compartment to the left and remove the cover. • Insert 9 V Alkaline battery (2-16/6) into the...

  • Page 41: 2.8.5 Attaching Darkfield Condenser

    Start-Up Carl Zeiss Attaching condensers Axiostar plus 2.8.5 Attaching darkfield condenser The darkfield condenser frame (for Axiostar plus) is required to place darkfield condensers in the condenser carrier. • Place darkfield condenser (2-17/6) in the darkfield condenser frame (2-17/5) and screw on fastening ring (2-17/7) The dry darkfield condenser (0.7/0.85, 465506-...
  • Page 42 Start-Up Axiostar plus Darkfield condenser Carl Zeiss Selection of recommended combinations: darkfield condenser – objective for best contrast when using transmitted-light darkfield technique Recommended darkfield condensers: Ultracondenser 1.2 / 1.4 465500-0000-000 (applicable from 0.75 - 1) Dry darkfield condenser 0.8 / 0.95 465505-0000-000 (applicable from 0.6 –...

  • Page 43: Attaching Colored Disk Carrier, Polarizer Or Wide-Angle Unit

    Start-Up Carl Zeiss Attaching colored disk carrier, polarizer or wide-angle unit Axiostar plus Attaching colored disk carrier, polarizer or wide-angle unit The polarizer must be installed if you want to use polarization contrast. The wide-angle unit must be used with objectives having a magnification <...

  • Page 44: Inserting White-Balance Filter

    Start-Up Axiostar plus Inserting white-balance filter Carl Zeiss 2.10 Inserting white-balance filter The white-balance filter can be used to balance differing, subjective color perceptions when observing a specimen and to increase contrast. The white-balance filter is only for subjective balancing for the user. For microphotography, remove the white-balance filter and use an appropriate conversion filter (e.g.

  • Page 45: Attaching Epi-Fluorescence Illuminator

    Start-Up Carl Zeiss Attaching epi-fluorescence illuminator Axiostar plus 2.11 Attaching epi-fluorescence illuminator The epi-fluorescence illuminator can be used only conjunction with binocular tube/phototube 30°. • If necessary, remove the 45° tube or 30° tupe and adapter for 30° tubes. •...

  • Page 46: Filter Stop Carrier For Epi-Fluorescence Illuminator

    Start-Up Axiostar plus Attaching epi-fluorescence illuminator Carl Zeiss 2.11.1 Filter stop carrier for epi-fluorescence illuminator The epi-fluorescence illuminator has two slots for filter stop carriers. The carriers can be inserted and accessed from the right or left. Filter stop carrier 1 (designated with one dot) has three positions (a, b, c) for filters with a 25 mm diameter.

  • Page 47: Attaching Hbo 50 Fluorescent Lamp

    Start-Up Carl Zeiss Attaching epi-fluorescence illuminator Axiostar plus 2.11.2 Attaching HBO 50 fluorescent lamp • Place HBO 50 fluorescent lamp (2-22/1) on the connection supports on the epi- fluorescence illuminator (2-22/3), align and tighten with clamping screw (2-22/2) . •...

  • Page 48: Replacing Burner In Fluorescent Lamp

    Start-Up Axiostar plus Attaching epi-fluorescence illuminator Carl Zeiss 2.11.3 Replacing burner in fluorescent lamp • Turn off power supply for HBO 50. Remove HBO 50 plug from the socket on the power supply. Danger of getting burned! Allow lamp housing to cool off at least 15 minutes.
  • Page 49 Start-Up Carl Zeiss Attaching epi-fluorescence illuminator Axiostar plus • Hold the new burner on the labeled metal base and place it in the heat sink so that the reflective portion of the burner points The heat sink must be parallel to the lamp base.

  • Page 50: Adjusting Burner In Fluorescent Lamp

    Start-Up Axiostar plus Attaching epi-fluorescence illuminator Carl Zeiss 2.11.4 Adjusting burner in fluorescent lamp Since Axiostar plus epi-fluorescence illuminator has an adjustment guide (filter stop carrier 2, 2-24/5), the HBO 50 can remain on the illuminator while adjusting the burner.

  • Page 51: 2.11.5 Changing P&C Reflector Module

    Start-Up Carl Zeiss Attaching epi-fluorescence illuminator Axiostar plus 2.11.5 Changing P&C reflector module The reflector carriage in the epi-fluorescence illuminator must be removed to change the P&C reflector module. Removing the reflector carriage: • Push the reflector carriage lever (2-25/5) to the left or middle position.
  • Page 52 Start-Up Axiostar plus Attaching epi-fluorescence illuminator Carl Zeiss Inserting a P&C reflector module: • Hold the module (2-26/4) with thumb and index finger as shown in Fig. 2-26 and insert at a slant from the top with the right and left holding supports (2-26/5) into the bottom spring clamps (2-26/2) in the reflector carriage (2-26/3).

  • Page 53: Changing Filter Set In P&C Reflector Module

    This compensates for or minimizes the already slight image shift when Zeiss filter sets are used between modules. 2-30 B 40-815 e 12/01...

  • Page 54: Changing Color Splitter In P&C Reflector Module

    Start-Up Axiostar plus Attaching epi-fluorescence illuminator Carl Zeiss We recommend the following procedure if you need to install filters without direction markings (arrow): Filters with reflective, dielectric layers should be installed so that the reflective layer Points out for an excitation filter (in relation –...
  • Page 55 Start-Up Carl Zeiss Attaching epi-fluorescence illuminator Axiostar plus • Tip the top module half up (Excitation) (2-30/1) and remove the bottom module half (Emission) from the holding supports (2-30/5b). • Remove color splitter (2-30/2) and spring frame (2-30/3) from bottom module half.

  • Page 56: Connecting The Instrument To The Line

    Start-Up Axiostar plus Connecting the instrument to the line Carl Zeiss 2.12 Connecting the instrument to the line The microscope can be operated using line voltages of 100 - 240 V without conversion. • Connect the line cable with connector...
  • Page 57 Start-Up Carl Zeiss Axiostar plus 2-34 B 40-815 e 12/01 Artisan Scientific - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan-scientific.com...

  • Page 58: Operation

    Attaching cameras with other filter thread sizes ............3-26 3.11.4 Sony DSC-S70, DC-S75, DSC-S85 on digital camera adapter 44 M52x0.75, 000000-1108-984.....................3-26 3.12 Axiostar plus - Packing the mount................3-27 B 40-815 e 12/01 Artisan Scientific - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan-scientific.com...
  • Page 59 Attaching Sony "Digital Handycam DCR-PC100" camera ...........3-24 Fig. 3-16 Attaching Sony "Digital Still Camera DSC-S70"............3-25 Fig. 3-17 Inserting the Axiostar plus in the case ................3-28 B 40-81 e 12/01 Artisan Scientific - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan-scientific.com...

  • Page 60: Switching On The Instrument

    (switch in "I" position). The integrated 6 V 20 W long- life halogen lamp must also be on. The Axiostar plus microscope is supplied with factory-aligned illumination. The illumination need not be adjusted even when the lamp is On/off switch with integrated control lamp exchanged by the customer.

  • Page 61: Using The Transmitted-Light Brightfield Technique

    Transmitted-light brightfield Axiostar plus Using the transmitted-light 3.2.2 Transmitted-light brightfield brightfield technique configuration Each Axiostar plus microscope can be – 3.2.1 General principle configured to permit the transmitted-light Transmitted-light brightfield microscopy is the brightfield technique. most usual of all the optical microscopy When using the Abbe condenser with 5- –...
  • Page 62 Operation Axiostar plus Transmitted light brightfield Carl Zeiss • Swivel 10x objective into beam path via knurled ring (3-4/5) of the nosepiece. • Look through the fixed eyepiece of the binocular tube (3-4/6) first and focus on the object via the focusing drive (3-4/1).

  • Page 63: Using Transmitted-Light Phase Contrast

    Operation Carl Zeiss Transmitted-light phase contrast Axiostar plus Using transmitted-light phase contrast 3.3.1 General principle The phase contrast technique is ideal for examinations of thin, unstained specimens, e.g. culture cells. The phase contrast technique uses the optical modulators "phase stop and phase ring" and...

  • Page 64: Setting Transmitted-Light Phase Contrast

    Operation Axiostar plus Transmitted-light phase contrast Carl Zeiss 3.3.3 Setting transmitted-light phase contrast Requirements: As described in chapter 2, the microscope is – ready for operation and switched on according to section 3.1. microscope must – transmitted-light brightfield, as described in section 3.2.

  • Page 65: Using The Transmitted-Light Darkfield Technique

    Operation Carl Zeiss Transmitted-light darkfield Axiostar plus Using the transmitted-light This is one of the reasons why even fine darkfield technique structures can be resolved and appear bright on a dark background although they partially lie 3.4.1 General principle below the resolving power of the light microscope.

  • Page 66: Setting Transmitted-Light Darkfield

    Plan-Neofluar 2.5x – 10x and Achroplan 4x. Focusing drive Luminous field diaphragm Darkfield condensers can be placed directly in Dark field slider the Axiostar plus when you use the darkfield Aperture diaphragm lever condenser carrier. Chapter 2 offers an overview Centering screws for condenser Nosepiece...
  • Page 67 Operation Carl Zeiss Transmitted-light darkfield Axiostar plus • Setting darkfield contrast with dry Reset focus level with Z drive. Focus the darkfield condensers luminous field diaphragm with condenser drive. Open the luminous field • If necessary, swivel open the wide-angle diaphragm just above the viewing field unit, colored disk carrier, polarizer or λ...
  • Page 68 Operation Axiostar plus Transmitted-light darkfield Carl Zeiss • Setting darkfield contrast with immersion Close luminous field diaphragm by turning oil darkfield condensers luminous field diaphragm ring counterclockwise until it makes contact. • If necessary, swivel open the wide-angle unit, colored disk carrier, polarizer or λ...

  • Page 69: Using Transmitted-Light Polarization Contrast

    Operation Carl Zeiss Transmitted-light polarization Axiostar plus Using transmitted-light Birefringent substances are recognized by the polarization contrast fact that they display 4 bright and 4 dark positions between crossed polarizers after 3.5.1 General principle rotation specimen around 360°. Depending on the level of birefringence,...

  • Page 70: Sample Differentiation Between Gout And Pseudogout

    Operation Axiostar plus Transmitted-light polarization Carl Zeiss • 3.5.4 Sample differentiation between If necessary, correct the orientation of the gout and pseudogout analyzer to achieve an optimal, dark viewing field. • Move two polarizers to the dark position (analyzer NORTH-SOUTH-oriented, polarizer •...

  • Page 71: Setting Epi-Fluorescence

    Swivel the desired objective into place. You can get an overview of filter sets and • Search for the specimen location to be fluorochromes available from Zeiss at: observed in the transmitted light. If the 5- position Abbe condenser with turret is to be www.zeiss.de/micro...

  • Page 72: Setting Epi-Fluorescence

    Operation Axiostar plus Setting epi-fluorescence Carl Zeiss The first epi-fluorescence setting is significantly easier if you use the Plan-Neofluar 20x/0.50 objective and a strong fluorescent specimen. Demonstration specimens can be used first. • First leave the illumination beam closed; move the filter stop carrier 2 (3-10/2) to the closed position.

  • Page 73: Measurement Of Lengths

    Operation Carl Zeiss Measurement of lengths Axiostar plus Measurement of lengths Settings: Before the length measurement using the Before starting: microscope can be performed, the micrometer The measurement of lengths using the Axiostar or scale value of the used objective / eyepiece plus requires the following, for example: reticle combination must be determined.

  • Page 74: Attaching Ics Double Tube

    Operation Axiostar plus Attaching double tube Carl Zeiss Attaching double tube • Loosen knurled screw (3-12/4) on the stand and remove binocular tube, if available. • Remove dust cover from the underside of the tube. • Press the double tube (3-12/1) to the right in a slightly inclined position into the spring of the stand mount.

  • Page 75: Attaching Photomicrography Equipment

    Axiostar plus Attaching photomicrography equipment For the use of the photomicrography equip- The Axiostar plus microscope with phototube ment, please observe the relevant separate can be changed from observation to photo- manuals in addition to the information provided micrography via the control knob (3-13/7 and...
  • Page 76 T2 adapter for PENTAX (KA bayonet) 416011-0000-000 For detailed information on SLR cameras, please see the new operating manual B 40-046 e, “Photomicrography using 35 mm SLR cameras”, from Carl Zeiss. B 40-815 e 12/01 3-19 Artisan Scientific - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan-scientific.com...

  • Page 77: Attaching Mc 80 Dx Microscope Camera (Film Cassette 35 Mm)

    Operation Carl Zeiss Photomicrography equipment Axiostar plus 3.9.2 Attaching MC 80 microscope camera (film cassette 35 mm) • Loosen hexagonal screws (3-14/7) and remove dust protection cap (3-14/9) from the phototube. • Insert adapter 60 for microscope camera (3-14/5) (456006-0000-000) into phototube (3-14/6) and fix it using three hexagonal screws (3-14/7).

  • Page 78: Attaching Adapters For Video Cameras

    Attaching adapters for video cameras The following video adapters and video zoom and 3-chip CCD cameras to the phototube of adapters with interface permit the Axiostar plus. attachment of 1-chip b/w and color cameras Tube Adapter Cameras 456105-0000-000 000000-1069-414 456106-0000-000...
  • Page 79 Carl Zeiss Attaching adapters for video cameras Axiostar plus The connecting piece 60 - 44 also allows video the phototube of the Axiostar plus with 60 mm adapters with 44 mm interface to be used with interface. Video adapter Suitable for Comments (Cat.

  • Page 80: Attaching Adapters For Digital Compact Cameras

    Operation Axiostar plus Attaching adapters for digital compact cameras Carl Zeiss 3.11 Attaching adapters for digital compact cameras Tube Adapter Cameras COMPACT 000000-1096-522 000000-1108-984 XIOSTAR IGITAL PLUS CAMERAS PHOTO M52x0.75 M52x0.75 AMCOR TUBE WITH DERS WITH To be used with:...

  • Page 81: Digital Cameras With 37 Mm Filter Thread, E.g. Sony Dcr-Pc100 / Sony Dsc - S50

    Operation Carl Zeiss Attaching adapters for digital compact cameras Axiostar plus 3.11.1 Digital cameras with 37 mm filter thread, e.g. SONY DCR- PC100 / SONY DSC – S50 When delivered, the sliding mount with thread M37 (3-15/2) and the lens mount (3-15/4) are premounted.

  • Page 82: Digital Cameras With 52 Mm Filter Thread, E.g. Sony Dsc-S70, Dsc-S75, Dsc-S85

    • Tighten the set screw (3-15/3) again. No untrimmed image may be obtained when a camera adapter combination is used which is not recommended by Carl Zeiss. 3.11.2 Digital cameras with 52 mm filter thread, e.g. SONY DSC–S70, DSC-S75,...

  • Page 83: Attaching Cameras With Other Filter Thread Sizes

    No untrimmed image may be obtained when a • Set the automatic exposure control to time camera adapter combination is used which is priority. not recommended by Carl Zeiss. • Set the aperture as wide as possible (i.e. select a small f-stop number!). 3-26 B 40-81 e 12/01 Artisan Scientific - Quality Instrumentation ...

  • Page 84: Axiostar Plus - Packing The Mount

    Axiostar plus - Packing the mount • Pull the binocular tube (3-17/6) completely The portable case for the Axiostar plus can be apart and slide the rubber foam component used to transport the basic Axiostar plus (3-17/7) between the eyepiece tubes until equipment (stand with binocular tube 45°...
  • Page 85 Velcro fasteners Rubber foam component Condenser Binocular tube 45° Rubber foam component Holding flaps Stand Fig. 3-17 Inserting the Axiostar plus in the case 3-28 B 40-81 e 12/01 Artisan Scientific - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan-scientific.com...
  • Page 86 Care, maintenance and trouble-shooting Axiostar plus Contents, List of illustrations Carl Zeiss CARE, MAINTENANCE AND TROUBLESHOOTING Contents CARE, MAINTENANCE AND TROUBLE-SHOOTING..........4-2 Care and maintenance of the instrument ..............4-2 Troubleshooting ......................4-3 4.2.1 Changing the fuses ....................4-3 4.2.2 Changing the 6 V, 20 W halogen lamp ..............

  • Page 87: Care, Maintenance And Trouble-Shooting

    MAINTENANCE TROUBLE-SHOOTING Care and maintenance of the instrument When using the Axiostar plus in humid climatic Care of the Axiostar plus is limited to the zones, proceed as follows: following operations: • Cover the instrument with the dust cover •...

  • Page 88: Troubleshooting

    Care, maintenance and trouble-shooting Axiostar plus Troubleshooting Carl Zeiss Troubleshooting Troubleshooting for the Axiostar plus is described in further detail using the following two examples: − changing the fuses and − changing the 6 V, 20 W halogen lamp Further measures are summarized in the table under4.2.2.

  • Page 89: Changing The 6 V, 20 W Halogen Lamp

    Use the protective cover to hold the new 6 V, 20 W halogen lamp and insert both lamp pins carefully into the receptacles. The Axiostar plus microscope is supplied with factory-aligned illumination. In general, the illumination need not be adjusted even when the lamp is exchanged by the customer.

  • Page 90: 4.2.3 Troubleshooting

    Care, maintenance and trouble-shooting Axiostar plus Troubleshooting Carl Zeiss 4.2.3 Troubleshooting Problem Cause Remedy Vignetting or The vis/doc knob on the Move the vis/doc knob on the inhomogeneous image phototube is not in the correct phototube to the correct position...
  • Page 91 Care, maintenance and trouble-shooting Carl Zeiss Troubleshooting Axiostar plus Problem Cause Remedy Image aberration Condenser not set correctly Set condenser correctly, see p. 3-3, 3-4, 3-5 Nosepiece not correctly switched Correctly click-stop nosepiece to stop position Specimen is not fixed on the...
  • Page 92 Care, maintenance and trouble-shooting Axiostar plus Troubleshooting Carl Zeiss Problem Cause Remedy The 6 V, 20 W halogen Line cable not connected to the Connect line cable to the line and lamp does not function line make sure to check the...

  • Page 93: 4.2.4 Spares, Wearing Parts And Tools

    Care, maintenance and trouble-shooting Carl Zeiss Troubleshooting Axiostar plus 4.2.4 Spares, wearing parts and tools Description Cat. No. Application 6 V, 20 W long-life halogen lamp 380079-9690-000 for the integrated illumination of the Axiostar plus SW 3 ball-headed screwdriver 000000-0069-551 for changing the phototube SW 2 90°-offset hexagonal key...

  • Page 94: Requesting Service

    Microscopy Service as follows: electrical components of the Axiostar plus may Telephone: ++49 180 333 6 333 only be performed by Carl Zeiss service staff or Fax: ++49 7364-20 4939 specially authorized personnel. E-mail: Med-Mikro-Service@Zeiss.de...
  • Page 95 Care, maintenance and trouble-shooting Carl Zeiss Axiostar plus 4-10 B 40-815 e 12/01 Artisan Scientific - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan-scientific.com...

  • Page 96: Appendix

    Axiostar plus Appendix Carl Zeiss APPENDIX Index............................A-2 List of Abbreviations ........................A-4 Physical and Technical Units ......................A-5 Certification in Accordance with DIN EN ISO 9001 / DIN EN 46001 EC Conformity Declaration B 40-815 e 12/01 Artisan Scientific - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan-scientific.com...
  • Page 97 Appendix Carl Zeiss Index Axiostar plus Index Disposal............VII Double tube ..........3-17 Dust cover ........3-19, 3-20 Dust-protection glass ........3-3 Abbe Condenser ....2-14, 2-15, 2-16, .............3-4, 3-6, 3-7 Adapter....3-17, 3-18, 3-19, 3-21, 3-23 EMC..............VI Ambient conditions ........1-11 Epi-fluorescence ........3-14 Ametropia ..........2-11 Epi-fluorescence illumination.....2-22, 3-14...
  • Page 98 Appendix Axiostar plus Index Carl Zeiss LED illuminator ..........3-3 Service ............4-9 Line voltage........1-11, 2-33 Slider for darkfield ........3-8 Luminous-field diaphragm ....3-3, 3-4, Slider for phase contrast ....2-14, 3-4, 3-6 .............3-5, 3-7, 3-9 Slider for phase darkfield ......2-14 SLR camera..........3-18 Spares ............4-8 Magnification ..........1-7...

  • Page 99: List Of Abbreviations

    Appendix Carl Zeiss Abbreviations Axiostar plus List of Abbreviations A-Plan Achromatic objectives featuring improved image flatness (ICS line) suitable for eyeglass wearers Correction Blue (conversion filter) Charge Coupled Device EC conformity declaration CP-Achromat Achromatic objective (ICS line) Canadian Standards Association...

  • Page 100: Physical And Technical Units

    Appendix Axiostar plus Physical and Technical Units Carl Zeiss Single Lens Reflex wrench opening black-and-white slow-blow (fuse type) T2-Adapter standard adapter for 35 mm cameras magnification of the objective association of German electrotechnicians visual W 0,8 Whitworth-type thread (inch thread) 0.8...
  • Page 101 Appendix Carl Zeiss Axiostar plus B 40-815 e 12/01 Artisan Scientific - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisan-scientific.com...

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