Peak Integration - Agilent Technologies 4150 TapeStation system System Manual

Peak Integration

In DNA assays, concentration values are calculated using the area of the
sample peak compared to the known concentration of the upper marker.
The user must ensure that both marker and sample peaks are properly
integrated, by manually adjusting the peak when necessary. An example of
correct upper marker peak integration is shown below.
In assays with no upper marker (RNA, High Sensitivity RNA, Genomic DNA, and Cell-free
N O T E
DNA assay) quantification is calculated using the lower marker.
Figure 27
Agilent 4150 TapeStation System Manual
Correct upper marker integration for DNA ScreenTape assays. Figure shows
D1000 ScreenTape assay.
Good Measurement Practices

Peak Integration

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