When To Use A Micro Vacuum Degasser - Agilent Technologies 1100 Series Reference Manual

Optimizing Performance

When to Use a Micro Vacuum Degasser?

When to Use a Micro Vacuum Degasser?
For capillary LC application with our 1100 Series Capillary LC System
For high-pressure mixing pumps with low flow rates or when you have
following conditions:
• if your detector is used with maximum sensitivity in the low UV
wavelength range,
• if your application requires optimum injection precision
• if your application requires highest retention time reproducibility
(mandatory at flow rates below 0.5 ml/min)
• if your sample or detection is sensitive to dissolved oxygen in the mobile
phase (degradation).
• With a Fluorescence Detector.
Generally a degasser should be used when negative effects due to dissolved
gas in the mobile phase exceed the limits that are acceptable for the user.
Negative effects that can be caused by dissolved gas are:
• Unstable flow due to unstable pumping conditions. This may result in a
high ripple or high standard deviations of peak retention times and peak
areas especially at low flow rates.
• Baseline noise on detectors that are sensitive to changes in the refractive
index.
• Sample degradation.
• Fluorescence Quenching due to dissolved oxygen.
• Baseline drift in electrochemical detectors due to dissolved oxygen
especially in reduction mode.
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