Acquire Chromatographic And Spectral Data - Agilent Technologies 1100 series Quick Start Manual

Step
4 Optimize the spectrum by setting the
Fragmentation Amplitude.
5 Save the mass spectrum.
6 Isolate and fragment another mass.
7 Turn off isolation and fragmentation. • In the MS(n) tab, select Manual

Acquire chromatographic and spectral data

Steps
1 Start an acquisition to acquire
chromatogram data.
2 Stop the acquisition.
Agilent 1100 Series LC/MSD Trap System Quick Start Guide
Detailed Instructions
a Increase or decrease the amplitude
in increments of 0.1 until the
isolated ion is reduced to about 10%
of the initial intensity, and the
fragment ions are present in
significant amounts.
b Enter a new amplitude with:
•
the keyboard,
•
scroll-bar buttons, or
•
the mouse wheel.
• Save the desired mass spectra:
•
Click the Save a single profile
toolbar button, or
•
Press the F7 key, or
•
From the top menu, click
Acquisition > Save Profile Now.
• Repeat Steps 3-5.
MS(n) > All Off.
Detailed Instructions
Start the acquisition in ChemStation:
•
Click the Start button, or
•
From the top menu, select
RunControl > Run Method or
Run Sequence.
Stop the method manually:
•
Click the Stop button, or
•
From the top menu, select
RunControl > Stop Run.
Comments
• The saved file is named test0000.d.
• The saved file is named test0001.d.
Comments
• Make sure that the LC settings are
correct.
• Assume that you have entered the
appropriate sample information and
data file name into the Sample Info
tab.
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