Olympus FLUOVIEW FV1000 Setup Manual
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Olympus FLUOVIEW FV1000 Setup Manual

Olympus FLUOVIEW FV1000 Setup Manual

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Note
To conduct maintenance of this equipment safely as well as securely, be sure to read
through this repair manual and the instruction manual before starting work.
Always carry this manual while repairing this equipment and keep it safely after reading it.
(This manual serves as the contents for maintenance workers)
OLYMPUS CORPORATION
Scientific Solutions Technical Service Dept.
All rights reserved. Reproduction in whole or in part without written permission is prohibited.
FV1000/FV1200
Document ID: DID-06397
Set Up Manual
(License D)
FLUOVIEW Series
17.00

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Summary of Contents for Olympus FLUOVIEW FV1000

  • Page 1 Always carry this manual while repairing this equipment and keep it safely after reading it. (This manual serves as the contents for maintenance workers) OLYMPUS CORPORATION Scientific Solutions Technical Service Dept. All rights reserved. Reproduction in whole or in part without written permission is prohibited.

  • Page 2: Revision History

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Revision History Date Version Description Corrected by Prepared the first edition. Dec. 28, 2008 1.00 2.00 Installation postscript of safety ring. Mitake Jun. 23, 2009 Dec.19, 2011 3.00 Collection and add explanation etc. Hara Nov.09, 2012 4.00 Added FV1200 unit, IX83 &...

  • Page 3: Table Of Contents

    Document ID: DID-06397 Contents of this Manual Before using this manual ........................- 1 - 1. Work flow and standard time ......................- 2 - 2. Assemble system ..........................- 4 - 2-1. Setup anti-vibration table ....................- 4 - 2-2. Setup microscope ........................- 5 - 2-2-1.Setup IX81【Mount microscope】...
  • Page 4 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 3-1-5. Rotation Delay2 adjustment (2kHz bi-directional Scan of adjustment to Y-axis Galvano) ............................... - 52 - 3-1-6. Bi-directional Scan (aspect ratio) scan-range adjustment ........- 53 - 3-1-6-1. 1kHz Bi-directional Scan range adjustment ............- 53 - 3-1-6-2.
  • Page 5 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 9. Used tool ............................- 88 - 9-1.Jig and tool list ........................- 88 - 9-2.Measurement instrument list ....................- 91 - 10. Appendix ............................- 92 - 10-1. AOTF scanning field check and adjustment ..............- 92 - 10-1-1.

  • Page 6: Before Using This Manual

    It includes important safety information about the system. All maintenance, setup, or service requiring assemble or disassemble Laser unit to or from the FLUOVIEW system must be performed by an authorized OLYMPUS service representative. Concept of this manual *This manual is for the purpose of Installing FV1000/FV1200, and there for does not contain repair procedure.

  • Page 7: Work Flow And Standard Time

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 1. Work flow and standard time Following list is standard time during installation. Check setup flow according to installing system configuration. List 1.【FV1000/FV1200 setup, Standard time】 Ref. Necessary jigs and Standar Task Work item document tools d time 1.5~ Unpacking and...
  • Page 8 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 * Standard time above is effective when installing system after pre-setup at the factory. * Additional procedure and time to install option unit are explained in License C manual. * Short plug have to be brought back after disconnecting from LD559. * To avoid ESD damage, connect to power supply immediately after disconnecting the plug.

  • Page 9: Setup Anti-Vibration Table

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 2. Assemble system 2-1. Setup anti-vibration table Necessary jig and tools Spanner, Level Purpose of work Remove shipping restrains and make it operational with air Necessity to check on check None sheet Cautions during work Be sure safety work when heavy product is handled. (Note) Procedure Picture/ GUI...

  • Page 10: Setup Microscope

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 2-2. Setup microscope IX3-FP (For IX83 microscope), IX2-FP(for IX81)､FV5-UVTAD(for BX61)､ Necessary jig and tools Waterproof cover (for BX61WI) ､ FV10-AVT(anti-vibration table) ､ Attached screws to fix anti-vibration table, hex wrench Fix microscope with anti-vibration table and setup other units such as Purpose of work objective lens.

  • Page 11: 2-2-2.Setup Bx61【Mount Microscope

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 2-2-2.Setup BX61【Mount microscope】 Procedure Picture/ GUI Remove 4 rubber feet from underneath of BX61 as shown right Fig. (See right Fig.) Place attached metal foot coming with Rubber foot FV5-UVTAD (See right Fig) Place and fix attached 3 metal parts at instructed position with black circle on anti-vibration table (2 parts which have elongate hole must...

  • Page 12: 2-2-3.Setup Bx61Wi【Mount Microscope

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 2-2-3.Setup BX61WI【Mount microscope】 Procedure Picture/ GUI Refer right Fig., mount BX61WI at black circle position on anti-vibration table. To fix 2 screw holes at back side of BX61WI, use BX61WI’s holes that are inside of Y-shaped part. Remove blind seal (White) at Y-shaped part when they are used.

  • Page 13: Ix83 Setup [Secure The Microscope]

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 2-2-4. IX83 Setup [Secure the microscope] Procedure Picture/ GUI Place the rings included with the IX3-FP to the bottom of the IX83 main unit on the four rubber feet. Secure the main unit so it does not come loose when placed on the vibration removal board.
  • Page 14 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture/ GUI Install on the anti-vibration table ⑲○○●○○●○○○ - If FV12-HSD is attached, secure the microscope ： ： ： ： using the four holes on the anti-vibration ⑦○○○○○○○○○ table. ： ： ： ： Refer to the four black circles in the diagram ①○○●○○●○○○...

  • Page 15: Release The Microscope Transport Lock [Ix83/Ix81/Bx61/Bx61Wi]

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 2-2-5. Release the Microscope Transport Lock [IX83/IX81/BX61/BX61WI] Procedure Picture/ GUI [Release the transport lock] IX81 Release motorized revolving nosepiece transport lock Loosen and remove the fastening device screw (1) using the hex screwdriver. Turn the fastening device nut (2) in the direction of the arrow and turn the fastening device axis (3) in the direction of the arrow until it is at the very bottom.

  • Page 16: Mounting The Microscope Unit [Ix83]

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure The mounting of any other items possessed by the user other than those shipped from the factory with the unit including condenser optical elements, fluorescent cubes, etc. must be registered with the IX83 TPC; Touch Panel Controller. For changing the settings for the IX83 TPC;...
  • Page 17 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture/ GUI Press in the direction of the arrow. (Push inside as seen from the person assembling the unit.) Use the included hexagon socket screws (four) and firmly tighten using Allen Key wrench.  AB5X12SA(4) Mount the stage so that the stage and microscope are the same lengthwise (laterally).
  • Page 18 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture/ GUI Mount the objective lenses Mount the objective lenses starting from the lowest magnification ordered in the first revolver hole. Start the FV10-ASW and check the position using “MICROSCOPE CONFIGURATION” or the touch panel controller.
  • Page 19 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture/ GUI Mount the observation tube and eyepiece lens Mount the fluorescence mirror unit (IX3-RFACA) Turn the fastening knobs ‘a’ on the side of the IX83 microscope to remove the knobs securing the cover and the cover itself.
  • Page 20 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture/ GUI Secure the IX3-RFACA using the hex screwdriver. Mount the cover to the IX83. Connect the interface cable to the IX3-RFACA. [Installation of the Inter Lock switch] Remove the plate (JA779200) shown in the figure from the IX83.
  • Page 21 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture/ GUI Insert the interlock switch included with the FV12-SUIX into the side of the IX83 main unit side Insert board. Secure the switch while pressing it in the direction of the arrow shown in the figure on the right.
  • Page 22 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture/ GUI Turn on power to the IX83. 1. Turn on the main switch to the IX3-CBH. 2. Turn on the main switch to the touch panel controller. 3. The black start screen will be shown for around 20 seconds. 4.
  • Page 23 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture/ GUI Close the cover by holding the mirror unit turret cover knob. If the cover is left open, the turret cannot be initialized. In addition, you will not be able to observe through the microscope because the illumination light is being shielded.

  • Page 24: Assemble Microscope Unit

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 2-2-7. Assemble microscope unit Procedure Objective lens should be mounted from lower power revolver number 1. (Since software registration from factory is same as above) Mount objective lenses after checking particle and scratch on mounting surface of revolver’s hole.

  • Page 25: 2-3.Setup Units For Fv

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 2-3.Setup units for FV Necessary jig and tools hex wrench Purpose of work Setup units for FV Necessity to check on check None sheet Cautions during work Mount SU’s foot according to its label and check repeatability of height (Note) when SU connect with microscope.

  • Page 26: Mount Fv10-Su/Fv12-Su With Microscope [Bx61, Bx61Wi]

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture/ GUI F10-RFACA fixing screw Insert FV10-RFACA to filter cube cassette slot and fix it with set screw. Tighten with hex wrench attached with microscope. (See right Fig.) 2-3-2. Mount FV10-SU/FV12-SU with microscope [BX61, BX61WI] Procedure Picture/ GUI [Common for FV1000/FV1200]...

  • Page 27: Assemble The Fv12-Su And Microscope [Ix83]

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture/ GUI From left side, insert FV10-SHUBX to AN slot of FV10-RFABX / RFAWI. (See right Fig1) Fig.1 FV10-SHAUBX is fixed to FV10-RFABX / RFAWI with set-screw in it for Laser Safety. (See right Fig2) Fig.2 2-3-3.
  • Page 28 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture/ GUI Adjust the height of the SU legs so that they are the same height as the tube lens for the mounted light projecting tube lead-in in step 2. The set screws on the bottom of the feet will turn when loosened.

  • Page 29: Fv10-Td Setup

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 2-3-4. FV10-TD Setup Procedure Picture/ GUI Transmitted lamp house Insert transmitted lamp house to FV10-TD and fixes it by set-screw as shown on right picture. Figure 1 Fix the optical fiber with both FV10-TD and F5-TDILL with set screw. Figure 2 【Connect with IX81】...

  • Page 30: 2-3-5.Fv10-Fir Setup

    Adjust evenness of illumination by using target Jig. * Refer FLUOVIEW FV1000 User’s Manual [Hardware Manual] ⅡPREPARATION For OPERATION Page 3-1 Attach Epi-Fluorescence optical-fiber to the adapter in FV10-FIR and Epi-Fluorescence port that is on side surface of FV10-SU.

  • Page 31: U-Hglgps Setup

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 2-3-6. U-HGLGPS Setup 【Used products】 ・Light Source（U-HGLGPS） ・Light guide adapter（FV12-HGAD） ・・・Component units： F12-LLGAD, F12-SHU F12-LLGAD F12-SHU ・Adapter for light guide illumination system ・Liquid light guide（U-LLG150/U-LLG300） （U-FFWADP） Procedure Picture/ GUI [FV1200] The following preparation is necessary because the diameters of the U-HGLGPS fiber and fiber port of the FV10-SU differ.
  • Page 32 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture/ GUI [For the IX3-ZDC specifications] Attach the spacer for reflected light illumination to the U-FFWADP. ・Set screw Attach the F12-SHU to the U-FFWADP (or fluorescent light adapter). ・Set screw Attach the U-FFWADP to the F12-SHU. ・Set screw Attach the light guide adapter to the U-FFWADP.
  • Page 33 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture/ GUI When the light guide end is fully inserted, secure it using the two locking screws with the F12-LLGAD. - AHU6X6SA(2) Loosen the set screw indicated by the arrow in the diagram and secure it in the SU fiber port. - 28 -...

  • Page 34: 2-3-7.Setup Power Supply Of M-Ar Laser And Ld559

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 2-3-7.Setup power supply of M-Ar laser and LD559 Procedure Picture/ GUI <In case of FV5-LAMAR laser which is made by Melles Griot> Adjust volume at front panel of power supply of M-Ar laser (rotate clockwise direction). 【Trouble case】...
  • Page 35 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 ☆ Setting up the FV5-LAHEG-1-2/FV10-LAHEG230-1 HeNe-G laser combined with a FV10-MCOMB/S-2 laser combiner HeNe-G lasers are manufactured by Melles Griot as of July 2014 as a result of the transfer of production from S.O.C. to Melles Griot. The FV5-LAHEG-1-2 is intended for use in the 100-V areas (exclusively for use in Japan) while the FV10-LAHEG230-1 is intended for use in both 115-V and 220-V areas.

  • Page 36: 2-4.Confirm How To Place Units

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 2-4.Confirm how to place units Necessary jig and tools None Purpose of work Place microscope and units for FV (According to recommended layout) Necessity to check on check None sheet Cautions during work Basically recommended layout, place units based on space and utility (Note) layout at installing site.
  • Page 37 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 【Recommended layout1.】Standard Spec.(e.g. Configuration with BX) [Caution] See following caution for layout laser power supply * [Caution] ◆ For laser power supply In case configuration of one laser power supply, place it on system desk as shown above Fig. In case configuration of two laser power supply, place LD559 power supply on system desk and place M-Ar laser power supply under anti-vibration table.
  • Page 38 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 【Recommended layout 2.】ASU Configuration (e.g. Combination with FV10-MCOMB) 【Layout of bottom base】 Bottom base - 33 -...
  • Page 39 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 【V1200 Recommended layout 3.】IX83 Microscope Assembly Example FV1200-IX83 Microscope and ASU Assembly - 34 -...

  • Page 40: Connect Cables

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 2-4-2．Connect cables Things to prepare Nothing Purpose of work Connect cables to each unit Register installation check None sheet Be careful of the core of cables with ferrite. Be careful of the order the cables are connected to each unit if used with the FV12-HSD.
  • Page 41 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Connect the BNC connector of the KW007100 cable unit to the DW547800 cable unit BNC connector “No.15.” The old version of the DW547800 cable unit has two blue BNC connectors. Take care not to connect it to the wrong connector.

  • Page 42: Connect Cables With Fv10-Pc And Fv12-Ctl

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 2-4-3. Connect cables with FV10-PC and FV12-CTL 【FV1000/FV1200】 FV10-PC can control IX81/BW61/BX61WI Microscopes. (IX83 is NOT controllable) The connection to FV10-EVA/FV10-MSASW uses COM port or USB port and U-USB232. Work precautions (Remarks) 【FV1200】 FV12-CTL is equivalent to IX83/IX81/BW61/BX61WI Microscopes.(FV10-ASW Ver.4 or later) The connection with IX3-CBH and FV12-CTL uses a C1394A (IEEE1394) cable.
  • Page 43 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 The setting of the dual display The screen property recognizes primary monitor and secondary monitor conventionally but in case of using”ATI Caralyst Control Center” two monitor can be recognized as one monitor. Procedure Picture/ GUI Click the right button on the disk top and choose "Screen resolution".
  • Page 44 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 * OLFV-z420 series. 【FV12-CTL-SET】 In case of connecting with one monitor Connect “Dual link cable” to DVI connector In case of connecting with two monitors Connect “Single link cable” to DVI connector and left side monitor Connect “DVI cable”...

  • Page 45: 2-5.Check For Starting Up Software

    USER: Administrator Password: FLUOVIEW [LOGIN to Windows in FV12-CTL-SET] When LOGIN window is displayed, log in as followings. USER :olympus Password :olympus After Windows starts up, launch FV10-ASW software and Log in as followings. UserID :Maker Password :Access - 40 -...
  • Page 46 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture/ GUI When initialize finished without any error, this procedure completes. In case error occurs during initialization or software freezes, figure out its cause from timing of error or message on the dialog. - 41 -...
  • Page 47 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 2-6.Laser Power Check Necessary jig and tools None Purpose of work Check each LD output before starting system adjustment Necessity to check on check sheet Check laser power for each wavelength and they are higher than 85% of Standard value measured power at the facility In case SIM system, measure laser power by temporally mounting ASU’s...

  • Page 48: Adjustment For Fv10-Mcomb

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 2-6. Adjustment for FV10-MCOMB 2-6-1. Inside layout Broadband Fiber for Main LD405 Broadband Fiber for ASU LD440 LD473 LD635 LD559 Multi-AR Laser - 43 -...

  • Page 49: Checking Location For Laser Power

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 2-6-2. Checking location for laser power Necessary jig and tools Laser power meter In case Laser power is lower than specification on ‘Laser power check’ Purpose of work in pre-step, adjust optical in MCOMB to optimize laser power for SU and Necessity to check on check None sheet...
  • Page 50 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Scan and laser setting are followings when measuring laser power. Acquiring image condition through Maintenance software: Scan size 4096x4096, Zoom 50x, Scan speed 200um/pixel, laser power 100% Laser setting through Maintenance software: “100%” and “Blanking setting is off” for all lasers [FV10-MCOMB/D] After...

  • Page 51: Optical Adjustment

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 2-6-3. Optical Adjustment Each laser has individual optics in order to align angle and position. In case laser power is less than specification, adjust corresponded optics according to checking location of laser power, 2-6-2. [Procedure] Find well aligned laser which the power meet specification at every checking location and use it as reference.

  • Page 52: Adjust System

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 3. Adjust System 3-1. Main SU Scan Field adjustment Necessary jig and Grid Chart (FVKC002), UPLAPO40x-Dry(or User’s Ob40x), Standard Eyepieces tools (KN0048) Set scanning range to FN18 and center of scan images to eyepiece’s center Purpose of work by adjusting “Gain”...
  • Page 53 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Adjust HV to make good contrast of scan images. Rotate the grid chart and make it parallel with edge of images if the scan image is rotated. (Make sure the center position of the grid by using KN0048 eyepieces after the grid chart specimen was moved.) * This adjustment would be easier to display Grid by clicking “2D Control Panel”...

  • Page 54: Scanner Delay1 Adjustment (1 Khz Bi-Directional Scan Of Adjustment To X-Axis Galvano)

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 3-1-2. Scanner Delay1 adjustment (1 kHz Bi-directional Scan of adjustment to X-axis Galvano) Necessary jig and Grid chart (FVKC002), UplanApo40xdry (or user’s Ob.40x) tools Purpose of work Jitter adjustment for 1kHz Bi-directional Scan Necessity to check on check sheet Standard value Jitter for 1kHz Bi-directional Scan <...

  • Page 55: Rotation Delay1 Adjustment (1 Khz Bi-Directional Scan Of Adjustment To Y-Axis Galvano))

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 3-1-3. Rotation Delay1 adjustment (1 kHz bi-directional Scan of adjustment to Y-axis Galvano)) Necessary jig and Grid chart (FVKC002), UplanApo40xdry (or user’s Ob.40x) tools Purpose of work Jitter adjustment for rotated 1kHz Bi-directional Scan (Y Galvano) Necessity to check on check sheet Standard value...

  • Page 56: Scanner Delay2 Adjustment (2 Khz Bi-Directional Scan Of Adjustment To X-Axis Galvano)

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 3-1-4. Scanner Delay2 adjustment (2 kHz Bi-directional Scan of adjustment to X-axis Galvano) Necessary jig and Grid chart (FVKC002), UplanApo40xdry (or User’s Ob.40x) tools Purpose of work Jitter adjustment for 2kHz Bi-directional Scan Necessity to check on check sheet Standard value Jitter for 2kHz Bi-directional Scan <...

  • Page 57: Rotation Delay2 Adjustment (2Khz Bi-Directional Scan Of Adjustment To Y-Axis Galvano)

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 3-1-5. Rotation Delay2 adjustment (2kHz bi-directional Scan of adjustment to Y-axis Galvano) Necessary jig and Grid chart (FVKC002), UplanApo40xdry (or user’s Ob.40x) tools Purpose of work Jitter adjustment for rotated 2kHz Bi-directional Scan (Y Galvano) Necessity to check on check sheet Standard value Jitter for rotated 2kHz Bi-directional Scan (Y Galvano) <...

  • Page 58: 1Khz Bi-Directional Scan Range Adjustment

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 3-1-6. Bi-directional Scan (aspect ratio) scan-range adjustment Necessary jig and Grid Chart (FVKC002), UplanApo40xdry(or User’s Ob.40x) tools Purpose of work Scan range adjustment for 1kHz and 2kHz bi-directional scan. Necessity to check on check sheet Standard value Shift from Normal Scan <...
  • Page 59 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Move to the upper part of Ratio/Concentration window. Select “Live” from Image drop down list. In Equation setting select “C” as a background from drop down list. The image like right figure appears when scan starts with opening Ratio/Concentration window.

  • Page 60: 2Khz Bi-Directional Scan Range Adjustment

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 3-1-6-2. 2kHz Bi-directional Scan range adjustment Procedure Picture / GUI Acquire reference image by normal scan Mode …Normal Scan (XY) Scan Speed …Fast Scan Size …256×256 *1) Zoom …6X Rotation …0 *Those values are different from that of 1 kHz scan Set the scanning condition as follows.

  • Page 61: Su Scan Field Automatic Adjustment. (Form Fv10-Asw Ver.1.7 Effective)

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 3-1-7. SU Scan Field automatic adjustment. (Form FV10-ASW Ver.1.7 effective) This automatic adjustment contains step 3-1-1 to 3-1-6, if it’s succeeded, those adjustments can skip. [Caution] No check on Kalman [Caution] Sim Light Path is / Sequential OFF (NOT pushed) (2) Scan Size 1:1 Zoom 1x...

  • Page 62: 3-2.Asu Scan Field Adjustment

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 3-2.ASU Scan Field adjustment Necessary jig and Grid Chart (FVKC002), UPLAPO40x-Dry(or User’s Ob40x), Standard Eyepieces tools (KN0048) Adjust ASU image matches with SU image by adjusting “Gain” and “Offset” Purpose of work for ASU Galvano motor. Necessity to check on check sheet Shift between SU image and ASU image...
  • Page 63 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Start [XY Repeat] scanning. Adjust laser power to make good contrast of scan images. (PMT HV is constant) If the scan images have shifted in the rotation direction, move "Rotation Offset" in “Scanner Parameter”...

  • Page 64: Scanner Delay Adjustment

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 3-2-2. Scanner Delay adjustment Procedure Picture / GUI Scanner Delay Adjustment Stop “Scanning”. Change the value of "Scanner Delay. (Scan images will be moved 1 pixel when the value of “Scanner Delay” is changed “20”.) Start “Scan” once and change “Scan Speed” on ASW, and then return to original scan speed again.

  • Page 65: Adjust Asu Image To Su Image

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 3-2-3. Adjust ASU image to SU image Procedure Picture / GUI Change the light path to “main SU” side. (Click “SIM Light Path” button on [Image Acquisition Control] window.) Set "Scan Pixel" to "1:1", “Scan Size” to “800x800”, “Fast Speed”...
  • Page 66 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Move [Offset]. [X], and [Y] of “Electric trimmer” on FV10-ASU side, and they are adjusted so that the value of “Center Pos. X/Y” on [Real Time Monitor] be same as SU’s value. Ar-488(or 473) laser for main SU and LD405 laser for ASU are used by turns, and then make sure there is no gap between images of main SU and ASU.

  • Page 67: 3-2-4.Asu Scans Field Automatic Adjustment. (Form Fv10-Asw Ver.1.7 Effective)

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 3-2-4.ASU scans Field automatic adjustment. (Form FV10-ASW Ver.1.7 effective) This automatic adjustment contains step 3-2-1 to 3-2-3, if it’s succeeded, those adjustments can skip. (3) Change to SIM light path and click Imaging. Acquire sequential image by clicking XY Repeat (2) Scan Size 1:1 Zoom 1x...

  • Page 68: Evenness Alignment Of Laser Illumination

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 4. Evenness alignment of laser illumination Necessary jig and Grid Chart (:FVKC002), UPLAPO40xDry(or User’s Ob.40x) tools Purpose of work Centering of the Laser spot Necessity to check on check sheet Evenness of illumination: Decreased illumination value in edge of transmitted images should not less than 50% of centers.
  • Page 69 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Take Transmitted images by using FLUOVIEW software and click “Ctrl and H” to change LUT (:Look-up table) to be “Hi-Lo” mode. *Note image do NOT become red in case the intensity is too dark Check the transmitted images and make sure the evenness of the laser illumination.

  • Page 70: Evenness Alignment (Laser For Asu)

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 4-2. Evenness alignment (Laser for ASU) Procedure Picture / GUI Change light path to ASU side in ｢Light Path｣ menu on ASW. Turn off all of the lasers for imaging that connected to main SU. Turn on the laser that connected ASU in ｢Laser｣ menu on ｢Stimulus Setting｣...

  • Page 71: Pinhole Position Adjustment

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 5. Pinhole position adjustment Necessary jig and Green fluorescence plate (FVKC003), UplanApo10x (or User’s Ob.10x) tools Purpose of work Adjust BSF (Beam Shifter) to align pinhole position to optical center. Necessity to check on check sheet Standard value Average of Image Intensity is maximum at each DM position Cautions during work...
  • Page 72 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI (※Unnecessary to change focus (1) Adjust “HV” of PMT to make good contrast of the position, 50μm from brightest images. And then adjust the focus position, where position. Because, reflection of the images are brightest. 488(or 473) laser is removed by SDM510.) (2) Click “Stop”...

  • Page 73: Manual Adjustment

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 5-2. Manual Adjustment Procedure Picture / GUI Acquire image according to step 5-1(1) to (7). Open “Real Time Monitor” window from “Maintenance” software and click “View” button at “Image Intensity” window. Adjust ”Pinhole Setting” on ”Pinhole” tab of “Maintenance”...

  • Page 74: Grating Adjustment

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 6. Grating adjustment Necessary jig and Mirror sample, UplanApo40xdry (or User’s Ob.40x) tools ◆ Grating position alignment Using 3 types of laser, adjust control signal for Galvanometer mirror that manipulating the “Grating”. Checking reflection of lasers by mirror, adjust ｢Offset｣ of galvano Purpose of work with 633(635) laser and then ｢...
  • Page 75 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Click “XY Repeat” button, start scanning. Adjust focus position to surface of the mirror. Adjust Z height of stage to make image the brightest. Click “Stop” button to stop scanning. Un-check CHS3, and then choose CHS1 (or CHS2) Set DMs and Barrier filters in “Light path”...
  • Page 76 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Click “XY Repeat” to start scanning. Adjust “Offset” for of CH1 (or CH2) in “Electric Trimmer” menu on “SPD” tab, and find the brightest position of the scanning images. Check the value of “Average” of “Image Intensity” on “Real Time Monitor”...

  • Page 77: Gain Adjustment@Laser543 (Or 559)

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 6-2. Gain adjustment@Laser543 (or 559) Procedure Picture / GUI : This step has to do after procedure 6-1. Turn off the 633(635) laser and turn on 543(559) laser. Set “Spectral Setting” for CHS1 (or CHS2) as following.

  • Page 78: Fine Offset Adjustment@Laser488 (Or 473)

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 6-3. Fine Offset adjustment@Laser488 (or 473) Procedure Picture / GUI : This step has to do after procedure 6-1 and 6-2. Turn off 543(559) laser and turn on 488(473) laser. Set “Spectral Setting” for CHS1 (or CHS2) as following.

  • Page 79: Checking Adjusted Value

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 6-4. Checking adjusted value Procedure Picture / GUI : This step has to do after procedure 6-1, 6-2 and 6-3. Turn 488, 543 and 633-laser on. (473/559/635 for diode laser) Set ｢Spectral Setting｣ for CHS1(or CHS2) as following.

  • Page 80: Fine Offset (Pan) Adjustment For Spdm

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 6-5. Fine Offset (PAN) adjustment for SPDM Procedure Picture / GUI Turn off 543(559) and 633(635)-laser, only 488(473)-laser is turned on. Select 1ch, SPD1CH (or2ch, SPD2CH), on ｢Image Acquisition Control｣ window and set the diameter of pinhole to ｢50μm｣.
  • Page 81 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Adjust ｢Grating Galvo Offset (by PAN)｣ for CH1 (or CH2) on ｢SPD｣ tab, and find most brightest position of the scanning images. Check the value of ｢Average｣ of ｢Image Intensity｣ on ｢Real Time Monitor｣ window Click ｢Set｣...

  • Page 82: Checking Analog Offset

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 7. Checking analog offset Necessary jig and tools None This adjustment is to get back ground of image with condition of no Purpose of work laser incidence and no voltage on PMT. Necessity to check on check sheet Average intensity of image should be within followings.
  • Page 83 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Click “View” on “Intensity” part. Average intensity is calculated and displayed after each scan. Set Normal Scan/Fast Scan mode on “Scan Setting” of “Acquisition Setting” of FV10-ASW. Click main“XY Repeat” on “Image Acquisition Control”...

  • Page 84: Hdd Backup/Recovery Procedure 【Fv1200

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 8. HDD backup/recovery procedure 【FV1200】 This procedure is for HP PCs arranged by OT. When performing restore/recovery on other PCs, it is recommended to check with the manufacturer. This procedure describes how to create backup of the C-drive in the D-drive, how to copy it to the C-drive and how to recover the C-drive by using this data.
  • Page 85 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Select drive 'D' as a copy destination and click ’Next’ on the screen shown to the right. Clicking ’Start backup’ in the screen shown on the right starts the backup. - 80 -...
  • Page 86 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI During backup, the screen shown at the top right appears. When backup is complete, click 'No' in the screen shown at the center right. Click ’Close’ in the screen shown at the bottom right to complete the backup.

  • Page 87: Creation Of System Repair Disc

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 8-2. Creation of system repair disc It is possible that system restoration disc is required when HDD recovery. Procedure Picture / GUI Open the Control Panel and click ’Back up your computer.’ Click ’Create a system repair disc.’ Insert blank disc (CD or DVD) into disk drive.
  • Page 88 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Replace the D-drive and copy the ’WindowsImageBackup’ folder, which is found immediately under the drive C, to a location immediately under the D-drive. Go to No. 6. Replace the C-drive. Immediately after turning on the power to the PC, insert the system recovery disk into the disk drive.
  • Page 89 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI After the screen shown at the top right appears, the screen shown at the bottom right appears. Go to No. 11. Open the Control Panel and click ’Back up your computer.’ Click the ’Recover system settings or your computer’...
  • Page 90 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Click ’Advanced recovery methods’ on the screen shown to the right. Click ’Use a system image you created earlier to recover your computer’ on the screen shown to the right. To restart the PC, click ’Skip’ on the screen shown at the top right and click ’Restart’...
  • Page 91 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Recovery is executed and the screen shown to the right appears. Click ’Next.’ Select ’Select a system image’ and click ’Next’ on the screen shown to the right. Select the D-drive as a backup storage location and click 'Next' on the screen shown to the right.
  • Page 92 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Click ’Next’ on the screen shown to the right. Clicking ’Finish’ on the screen shown at the top right displays the screen shown at the bottom right. Click ’Yes’ to start the restore process. The restore process takes about 5 minutes.

  • Page 93: Used Tool

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 9. Used tool 9-1.Jig and tool list Registered Name Description Use for final adjustment with this Biological specimen image e.g. PTK2 Use for Grating adjustment Fluorescence beads sample Evaporated surface should be on top (not protected) Glass bottom dish As for ZDC, this is used to check AF function...
  • Page 94 Keep the slide in a dark place and wrap it in aluminum foil so that it is shielded from light. PLAPON60XO SC2/NA1.42 Use for performance of OSR (Olympus N4273900 Super Resolution) IMMOIL-F30CC Use for performance of OSR (Olympus...
  • Page 95 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Registered Name Description UPLSAPO60XS/NA1.30 Use for performance of OSR (Olympus N4212200 Super Resolution) SIL300CS-30CC Silicone Oil 300CS N4190800 Use for performance of OSR (Olympus Super Resolution) FV12-MHSY or FV12-MHBY Use for performance of OSR (Olympus Super Resolution)

  • Page 96: 9-2.Measurement Instrument List

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 9-2.Measurement instrument list Registered Name Description check level － Leveling instrument anti-vibration table UNIVERSAL USE STANDARD EYEPIECE Use to align grid chart through this KN0048 before Scan field adjustment 0.1mm / Scale Laser power meter set Use to measure laser power at multiple location HIOKI: 3664+9742...

  • Page 97: Appendix

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 10. Appendix 10-1. AOTF scanning field check and adjustment Necessary jig and tools Grid Chart (:FVKC002), UPLAPO40xDry(or User’s Ob.40x) AOTF’s output-timing adjustment based on starting and stopping Purpose of work position of scanning for both SU and ASU Necessity to check on check None sheet...

  • Page 98: Delay/Extent Automatic Adjustment For Su's Aotf

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 10-1-2. Delay/Extent automatic adjustment for SU’s AOTF *In case execute this adjustment continuing from step 10-1-1, (1) to (7) can be skipped. Procedure Picture / GUI Adjust microscope focus and condenser lens height as following condition. Sample：Grid Chart（FN18, 1:1）...

  • Page 99: Delay/Extent Manual Adjustment For Su's Aotf

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 10-1-3. Delay/Extent manual adjustment for SU’s AOTF *In case 10-1-2 adjustment is NOT completed, this procedure needs to be done with same condition. Procedure Picture / GUI Follow step (1) to (7) of procedure 10-1-2 to make adjustment condition.

  • Page 100: Asu's Aotf

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 10-2. ASU’s AOTF 10-2-1. Check laser-output-timing of ASU’s AOTF *This check has to be done after laser-output-timing adjustment of Su’s AOTF Procedure Picture / GUI Adjust microscope focus and condenser lens height as following condition. Sample：Grid Chart（FN18, 1:1） Objective lens: UPLAPO40XDry(or User’s Ob 40X) Choose TDch and click “SIM Light Path”...

  • Page 101: Delay/Extent Automatic Adjustment For Asu's Aotf

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 10-2-2. Delay/Extent automatic adjustment for ASU’s AOTF *This check has to be done after laser-output-timing adjustment of Su’s AOTF *In case execute this adjustment continuing from step 10-2-1, (1) to (7) can be skipped. Procedure Picture / GUI Adjust microscope focus and condenser lens height as following condition.

  • Page 102: Delay/Extent Manual Adjustment For Asu's Aotf

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 10-2-3. Delay/Extent manual adjustment for ASU’s AOTF *This check has to be done after laser-output-timing adjustment of Su’s AOTF *In case 10-2-2 adjustment is NOT completed, this procedure needs to be done with same condition. Procedure Picture / GUI Follow step (1) to (7) of procedure 10-1-2 to make adjustment condition.

  • Page 103: Setup Procedure Of Optional Unit

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 10-3. Setup Procedure of Optional Unit 10-3-1. LD405/440 for Option imaging 【FV1000】 Green fluorescence plate (FVKC003), fluorescence beads Necessary jig and tools Specimen(0.5um) 1.5mm hex wrench（2） Purpose of work Align FV10-COMB LD405/440 for imaging (FV10-COMB) with main lasers. Necessity to check on check sheet Standard value...
  • Page 104 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI FV10-ASW setting Check TD and 1ch, and choose laser. (Either 488nm or 515nm) Minimize pinhole sides. Set laser output 10% and click “XY-Repeat” button to start SCAN. Focus on green fluorescence plate and adjust HV for both TD and 1CH.
  • Page 105 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Move COL part of FV10-FUR to make bright part of image aligning with 1ch image that is adjusted on step 8. Repeat step 10 and 11 to make the brightest positions of TD and 1CH image that capture with LD laser aligning with they are captured with main laser.
  • Page 106 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Scan as XY-Z mode to get fluorescence bead’s image. Check excited beads image between LD and main laser No shift on XY direction（optical center position） No shift on XZ direction（Per-focal position） In case there is any shift on XY direction, repeat step 10 to 12.

  • Page 107: Mount Ix2-Zdc 【Fv1000

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 10-3-2. Mount IX2-ZDC 【FV1000】 Procedure Picture / GUI Lens ass’y 【Mount IX2-ZDC unit】 Disassemble lens ass’y from AF sensor unit. AF sensor unit Keep AF sensor unit leveled and insert it until it is stopped by contacting with ZDC mount. Rotating protector Fix set screw (a) at the position where rotation protector contacts with upper part of slotted hole of...
  • Page 108 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI 【Check AF】 Set glass bottom dish with marked sample. -1 Check AF functions from 150um upper of focus plane. -2 Check AF function from 150um lower of focus plane. Check AF function with FV10-ASW Click [ZDC Setting] of [Acquisition Setting] to open [ZDC Control] window.

  • Page 109: Mount The Ix3-Zdc [Fv1200+Ix83]

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 10-3-3. Mount the IX3-ZDC [FV1200+IX83] Procedure Picture / GUI [Remove the transport lock] Use the hex screwdriver included with the microscope to remove transport lock “a.” Be sure to store the locking screw in a safe place because it will be used when transporting the microscope.
  • Page 110 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Use the hex screwdriver to tighten the IX3-ZDC locking screws. Connect the IX3-ZDC cable connector to the IX83 connector. Notch filter for IX3-ZDC Raise the filter holder on the IX3-ILL and attach the notch filter for the FV12-ZDCBA.
  • Page 111 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture/ GUI Attach the FV12-IXCOV to the IX3-ILL filter holder. Align the FV12-IXCOV mounting holes with the mounting hole positions at the front and rear of the IX3-ILL filter holder. Secure the FV12-IXCOV with the screws supplied.
  • Page 112 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI [Continuous Focus Mode] 1. At the [MENU] screen, tap [Start Operation]. 2. At the [Operation] screen, open the [Continuous AF] tab. 3. Tap the [Start] button. The Z drift correction will begin. The machine will beep once when it is focused.
  • Page 113 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI [ZDC Control] window Explanation <Return to current pos> Moves to the location with the Z position drift error removed. <Move to offset pos> Removes the Z position drift error and moves to the set focus position by pressing the [Set Offset] button.

  • Page 114: Fv12-Hsd Setup [Fv1200]

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 10-3-4. FV12-HSD Setup [FV1200] Things to prepare Green fluorescent plate specimen (FVKC003), mirror specimen Purpose of work Mount the FV12-HSD unit to the FV10-FD/FV10-SPD Register installation check sheet Analog offset Normal :150±20 Standard values Fast :150±60 Photon count :100±3 Work precautions Adjust the analog offset after more than two hours have passed since...
  • Page 115 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Connect the cable to the FV12-HSD. [FV1200 IX81/BX63+FV12-HSD] ZCONT for PSU Connect the cables in the following order: PSU OP for SU (PSU-2)-SU-FV12-HSD. For FV10-SHAUIX/BX SHA for SHAU/OPD [For the FV1200 IXD/BXD specifications] Connect the cables in either of the following order: PSU (PSU-2)-SU-IXD or BXD or BXD-GAP-FV12-HSD.
  • Page 116 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI From the maintenance software, click “Real Time Monitor,” and then click “View” under “Intensity.” The intensity value will not be displayed if the Real Time Monitor is not started before the scan. Every time an image is scanned the image’s average intensity value will be automatically calculated.
  • Page 117 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Set the cursor for the maintenance software “Analog When the “Coarse” cursor is Offset” – “Coarse” side to the very left (where gradually moved to the right, there is the value is the smallest) and then gradually move it a place where the intensity value to the right (gradually raise the value).
  • Page 118 Turn off all other channels that will not be adjusted (remove the checkmarks). * If FV10-OSRASW (Olympus Super Resolution Imaging License) is included in the bundle to be sold, refer to Chapter 10-8, “The check method of OSR performance.” - 113 -...

  • Page 119: Mounting The Camera Adapter [Fv1200+Ix83]

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 10-3-5. Mounting the Camera Adapter [FV1200+IX83] Applicable Camera Adapters: U-TV0.3XC, U-TV0.2XC, and U-TV1XC Procedure Picture / GUI Remove the cap (AA837900) from the left side port of the IX83. Attach the Camera Adapter to the left side port of the IX83.

  • Page 120: Fv1200 Ix83 Specifications How To Terminate The System

    Terminate FV10-ASW software Tap “OFF” on the IX83 TPC. When “Sure to Exit?” is displayed, tap “OK.” Confirm that [OLYMPUS] is displayed, as shown in the figure to the right. Press the main switch of the TPC to turn it off.

  • Page 121: Set Down Procedure Of Fv1200, Ix83 Specification

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 10-5. Set Down procedure of FV1200, IX83 specification Procedure Picture / GUI Terminate FV10-ASW software Terminate FV12-CTL. * Do not terminate IX3-CBH and TPC unit. Tap "Start Operation" of the touch TPC. * See Instruction manual of IX83 to operate TPC. Open right side of cover on IX83.
  • Page 122 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Tap "OFF" of the TPC and finish OPRATION menu and Menu. 10-4. procedure FV1200 IX83 specifications SYSTEM end method, finish an FV1200 system. Attach the transportation lock screw in IX83. Remove the plate included with the interlock to the IX83.
  • Page 123 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Remove a IX83 from anti-vibration table. When handling the IX83, be sure to hold the base (front) “a” and the bottom of the illumination support “b” as shown in the diagram on the right. Other instructions Do not bent U-LLG150 less than 40mm radius.

  • Page 124: Placing The Button Function Stickers To U-Mcz

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 10-6. Placing the button function stickers to U-MCZ. - 119 -...

  • Page 125: Attachment Ferrite Core To U-Mcz Cable

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 10-7. Attachment ferrite core to U-MCZ cable * Corresponds from FV1200 IX83 System S/N 3K** of shipments after the March, 2014. - 120 -...

  • Page 126: The Check Method Of Osr Performance

    10-8. The check method of OSR performance. * FV10-OSRASW is image processing software. * The following steps are for checking the OSR (Olympus Super Resolution) performance of the FV1000/1200 as hardware. These steps do not involve FV10-OSRASW analysis of scanned images acquired through the FV12-HSD.
  • Page 127 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Attach a spectral cube (FV12-MHSY or FV12-MHBY) to the FV12-HSD. Attach the spectral cube by tightening the fixing screw (blue). Securely fasten the spectral cube to the dovetail. Attach the panel (JB094300) to the FV12-HSD. ・Screws (JA95860)(quantity: 4) FV12-MHBY or FV12-MHSY PANEL...
  • Page 128 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI 【Light Path & Dyes】 Excitation DM:473/559/635, 488/543/633, 488/559/635, etc. 1CH to 3CH_SDM: Glass Select a DM (excluding B20/80) that reflects 473-nm or 488-nm laser Click the “Through HSD Light Path” button. 【If the UPLSAPO60XS objective is used】 Adjust the correction collar of the objective while observing the brightness of the fluorescent beads and resolution power.
  • Page 129 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI ASW setting Zoom: Switch to 1x. Click the [XY-Repeat] button. Bring a fluorescent bead into focus while looking at the [View] screen. O.K. Adjust the laser intensity such that the center intensity of the fluorescent bead is somewhere between O.K.
  • Page 130 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Check the intensity value of the isolated bead. Enlarge the fluorescent bead the intensity of which is to be measured. Open [Intensity Profile]. Draw a horizontal ROI line through the isolated bead. Draw the line such that it passes through the center of the bead.
  • Page 131 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI 【Image Acq. Control】 Click the [Depth] button. 【Filter Mode】 Place a check mark in the Kalman checkbox. Select the Kalman Line radio button. Set Frame to “10.” Click the “XY-Z” button. Upon completion of the XYZ scan within the specified range, the options “Series Done”...
  • Page 132 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Visually check the created intensity profile. If the FWHM of the fluorescent bead diameter is 250 nm or less, proceed to the next step. If the FWHM exceeds 250 nm, draw a ROI line through another bead. How to check the FWHM 1.
  • Page 133 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI 2. Calculate the value equivalent to half the intensity. In the following figure, the value is 800 x 1/2 = 400 (Y axis). * The horizontal red arrows point at the points corresponding to this value. 3.
  • Page 134 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Repeat steps 19 through 21 to acquire intensity profiles of at least three fluorescent beads with FWHMs of up to 250 nm by drawing ROI lines through such beads. Then, save the .xls files. File name: any Destination: Desktop Save the XY-Z image used for the performance check.
  • Page 135 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI ※Profile ※Fitting Entry fields in “Fitting.xls” Profile_DATA Copy the data in B7:B31/C7:C31. Fitting_DATA Paste the profile data into B12:B36/C12:C36. Overwrite the Fitting data with the Profile data. Select “Data” in the Menu tab. Click the “Solver”...
  • Page 136 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI The “Solver Results” window appears. Confirm that the message “Solver has converged to the current solution. All constraints are satisfied.” is displayed in boldface. Click the “OK” button. Confirm that the “FWHM” value for J127:K12: Is equal to or less than the standard value for the PLAPON60XO SC2 objective, i.e., 206 nm;...

  • Page 137: Procedure For Adding Solver To Excel

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 10-9. Procedure for Adding Solver to Excel * This section describes how to add Solver to Excel assuming that Excel 2010 is used. * For information on how to add Solver to other versions of Excel, please refer to the Microsoft website.
  • Page 138 Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 Procedure Picture / GUI Click the “Data” tab and confirm that the “Solver” icon is displayed at the top right of the screen. * Reference 【Product Standard Excerpts】 List of objectives available in FV10-OSRASW super resolution mode Objective NA UPLSAPO60XW 1.35...

  • Page 139: Fv10-Lcd30【Eizo】Connection

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 10-10. FV10-LCD30【EIZO】connection Resolution settings with respect to each monitor and Connecting to a controller 1. For a controller in combination with two 19-inch monitors: Set the monitor resolution to 1280x1024 and connect it by DVI cable provided with monitor or by Display Port to DVI converter cable 2.

  • Page 140: Connecting To Fv12-Ctl-Set【Z440

    Document ID: DID-06397 FLUOVIEW Series FV1000/FV1200 10-11. Connecting to FV12-CTL-SET【Z440】 10-11-1. Monitor connection (1) In case of connecting with one monitor ・Connect cable to Display Port connector (2) In case of connecting with two monitors ・Connect cable to DVI connector ・Connect the DVI cable with DVI convertor connector to Display Port connector IEEE1394b connector Connect to CBH DVI connector...
  • Page 141 5301 Blue Lagoon Drive, Suite 290 Miami, FL 33126, U.S.A OLYMPUS (CHINA) CO., LTD A8F, Ping An International Financial Center No.1-3, Xinyuan South Road, Chaoyang District, Beijing. 100027 P.R.C. OLYMPUS KOREA CO., LTD. Olympus Tower, 446, Bongeunsa-ro (Samsung-dong), Gangnam-gu Seoul, Korea 135-509.

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