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GE EPS 3501 XL Operating Instructions Manual

Electrophoresis power supply
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GE Healthcare
Electrophoresis Power
Supply EPS 3501 XL
Operating Instructions
Original instructions

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  Summary of Contents for GE EPS 3501 XL

  • Page 1 GE Healthcare Electrophoresis Power Supply EPS 3501 XL Operating Instructions Original instructions...
  • Page 3: Table Of Contents

    Maintenance ................41 General ........................41 Troubleshooting ..............43 Reference information ............45 Specifications ......................45 Literature ........................45 Ordering information ................... 45 Appendix A Electrical and communication connections..47 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 4 Table of Contents Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 5: Introduction

    Do not operate EPS 3501 XL in any other way than described in the user documentation. If you do, you may be exposed to hazards that can lead to personal injury and you may cause damage to the equipment.
  • Page 6: Intended Use

    EPS 3501 XL is a high quality, high precision power supply for electrophoresis applications that require advanced programming and high voltage. EPS 3501 XL is intended for research use only, and shall not be used in any clinical procedures, or for diagnostic purposes.
  • Page 7: Regulatory Information

    Software texts and commands are identified by bold italic text. A colon is used to separate menu levels (e.g. File:Open refers to the Open option in the File menu). Regulatory information This section lists the directives and standards that are fulfilled by EPS 3501 XL. Manufacturing information Requirement...
  • Page 8: International Standards

    GE Healthcare. Regulatory compliance of connected equipment Any equipment connected to EPS 3501 XL should meet the safety requirements of EN 61010-1/IEC61010-1 or relevant harmonized standards. Within the European Union, connected equipment must be CE-marked. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 9: Instrument

    Two electrophoresis units can be connected to EPS 3501 XL and run with the same programmed method at one time. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 10 Introduction Instrument Instrument description Figure 1-1. The main parts of the EPS 3501 XL instrument. Part Description Display Keyboard Output sockets Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 11: Front Panel

    (LED) that lights when voltage is applied (HV on) and connectors for two electrophoresis units. Display Part Description Part Description Mode Breakpoint parameter Voltage Phase number Current Control mode (step) Power Program number Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 12 STOP. Press run to run the same method again or press set enter to choose another program, program a new method or make changes in an existing method. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 13 There are two sets of output sockets to allow two electrophoresis units to be connected and run at the same time. Output socket Label Color Voltage Positive 0 and +1750 V Negative Black or blue 0 and –1750 V Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 14 Introduction Instrument Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 15: Safety Instructions

    2.1 Safety precautions Introduction The EPS 3501 XL is powered by mains voltage and handles liquids that may be hazardous. Before installing, operating or maintaining the equipment, you must be aware of the hazards described in this manual. Follow the instructions provided to avoid personal injury or damage to the equipment.
  • Page 16 Safety instructions Safety precautions WARNING Do not use EPS 3501 XL if it is not working properly, nor if it have suffered any damage, for example: • damage to the power cord or its plug • damage caused by dropping the equipment •...
  • Page 17: System Operation

    Safety instructions 2 Safety precautions 2.1 WARNING Protective ground. The EPS 3501 XL must always be connected to a grounded power outlet. WARNING Power cord. Only use power cords delivered or approved by GE Healthcare. WARNING Access to power switch and power cord. Do not block the rear panel of the instrument.
  • Page 18: Labels

    Symbols used in safety labels Label Meaning Warning! Read the user documentation before using the system. Do not open any covers or replace parts unless specifically stated in the user documentation. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 19 This symbol indicates that the product contains hazardous materials in excess of the limits established by the Chinese standard SJ/ T11363-2006 Requirements for Concentration Limits for Certain Hazardous Substances in Electronics. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 20: Emergency Procedures

    Safety instructions Emergency procedures 2.3 Emergency procedures This section describes how to do an emergency shutdown of EPS 3501 XL. The section also describes the result in the event of power failure. Emergency procedures In an emergency situation, do as follows to stop the run:...
  • Page 21: Recycling Information

    Disposal, general instructions When taking EPS 3501 XL out of service, the different materials must be separated and recycled according to national and local environmental regulations. Recycling of hazardous substances EPS 3501 XL contains hazardous substances.
  • Page 22 Safety instructions Recycling information Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 23: Installation

    Installation 3 Site requirements 3.1 Installation EPS 3501 XL is delivered in protective packing material and shall be unpacked with great care. Any equipment connected to EPS 3501 XL must fulfill applicable standards and local regulations. For detailed information on Installation, see Electrophoresis Power Supply EPS 3501 XL User Manual.
  • Page 24: Connections

    Connections Communication Connect EPS 3501 XL according to the electrical drawings in Appendix A. Connect the leads from the electrophoresis unit to the output sockets on the EPS 3501 XL front panel (red to red, and black or blue to blue), see Section 1.3. The red lead is the positive and black or blue is the negative.
  • Page 25: Spare Parts And Accessories

    Note: Only 13 Amps fuse approved to B.S 1362 A.S.T.A. should be used. 3.5 Spare parts and accessories For correct up to date information on spare parts and accessories visit: www.gelifesciences.com\2DE Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 26 Installation Spare parts and accessories Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 27: Operation

    Operation 4 Operation overview 4.1 4 Operation 4.1 Operation overview The operation of the EPS 3501 XL is described in the following sequence. Programming or editing a method. Editing a method. Running a method. Programming and running are discussed in more detail in the following sections.
  • Page 28: Setting Up A Run

    Alarm Phase 1? Phase: - - - - Confirm by Select by enter 1000 Vh 1000 Vh SET: SET: 1000 V 10 mA 10 W 1000 V 10 mA 10 W Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 29 Choosing step means that voltage, current and power limiting values are programmed. The electrophoresis will be controlled by one of these limiting values, which means that it is run at either constant voltage, current or power. The EPS 3501 XL automatically switches over the controlling parameter according to programmed limits and conductivity variations in the system.
  • Page 30 A linear voltage gradient is made with zero (for the first phase) or the programmed endpoint of the phase before (for the next phases) as starting point and the programmed endpoint as Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 31 Setting up a run 4.3 endpoint. The electrophoresis will be controlled by this voltage gradient provided the limiting current or power is not attained. The EPS 3501 XL thus automatically switches over the controlling parameter according to the programmed limits and conductivity variations in the system.
  • Page 32 Last Phase? After programming the breakpoint you are asked if this is the last phase or not. Select Yes (Y) or No (N) with and confirm with set enter. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 33 If needed, change program number by entering the start position in SET mode with set enter or exit, changing the number with and confirming by set enter. Bypass the mode question with set enter. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 34: Optional Programming

    Manual. Choosing run parameters EPS 3501 XL is an automatic cross-over power supply that allows the user to set limits for voltage, current and power. It is also possible to program linear voltage gradients. During electrophoresis, only one of the parameters is limiting at a time. The limiting parameter determines, together with the conductivity in the electrophoresis system, the values for the other two parameters.
  • Page 35: Preparations Before Start

    A light emitting diode shows when voltage is applied (HV on). If no current is displayed or if “HALT: Low start current” is shown, check the electrical connections to the electrophoresis equipment. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 36 Choose Insert or Delete. Program a new phase 2. Back to new Phase 2 start position. Old phase 2 is deleted. Figure 4-4. Inserting and deleting a phase in a program. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 37 Press exit or pause continue to go back from P-SET to PAUSE. Press run or pause continue to proceed with the electrophoresis. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 38 Note that no values can be changed here. Only one phase is shown at one time. Switch to another phase by using . The display returns automatically to show RUN values after 5 s. Alternatively use exit or run. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 39 STOP. Disconnect the leads and proceed with post-electrophoretic techniques. Since diffusion will begin as soon as the voltage is turned off, you should remove the gel and begin staining, blotting or autoradiography immediately. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 40 Operation Performing a run Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 41: Maintenance

    General 5.1 5 Maintenance 5.1 General No user maintenance is required for Electrophoresis Power Supply EPS 3501 XL. NOTICE Cleaning. Keep the instrument dry and clean. Wipe regularly with a soft damp tissue and, if necessary, a mild cleaning agent. Let the instrument dry completely before use.
  • Page 42 Maintenance General Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 43: Troubleshooting

    If a serious error occurs, the program enters the FAIL mode. The output is switched off and an error message is shown in the display. FAIL:Code No: xxx Call service Please note the error code number and contact your GE Healthcare representative. Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 44 Troubleshooting Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 45: Reference Information

    84 to 106 kPa (840 to 1060 mbar) Acoustic noise level < 70 dB A Literature For further information regarding Electrophoresis Power Supply EPS 3501 XL, refer to the Electrophoresis Power Supply EPS 3501 XL User Manual. Ordering information For ordering information visit www.gelifesciences.com/2DE.
  • Page 46 Reference information Ordering information Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 47: Appendix A Electrical And Communication Connections

    Voltage 100-120/220-240 V AC Frequency 50/60 Hz Power 260 W 4 A Max MAINS Disconnect before servicing 100-120 220-240 Part Description Power switch Power inlet Voltage range switch Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 48 Appendix A Electrical and communication connections Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28-9639-69 AA...
  • Page 50 Sweden All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them. A copy of these terms and conditions is available on request. Contact your local GE Healthcare representative for the most current information.