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RWD C100-SE User Manual

Automated cell counter
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C100-SE Automated Cell Counter
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Summary of Contents for RWD C100-SE

  • Page 1 C100-SE Automated Cell Counter User Manual...

  • Page 2: Intellectual Property Right

    RWD have the right to use the instruction as confidential information. Any individual and/or organization shall not disclose the instruction of all or part of the information by any means without RWD’s written permission. Nor shall any other person or organization be allowed to obtain all or part of the information of this instruction manual by any means.

  • Page 3: Table Of Contents

    CONTENTS 1-INTRODUCTION............................1 1.1 O ............................. 1 VERVIEW 1.2 S ..............................1 AFETY 1.3 C ......................1 OMPREHENSIVE ESCRIPTION 1.4 P ..........................2 RODUCT EATURES 1.5 E ......................2 NVIRONMENTAL EQUIREMENTS 1.6 P ........................... 2 RODUCT ARAMETER 1.7 U ........................3 SER SETTING ARAMETER 1.8 P...
  • Page 4 5.1 B ......................... 14 RIGHT IELD SSAYS 5.1.1 Focusing ............................16 5.2 S ..........................19 AVING ESULTS 5.3 P ..........................20 REVIOUS OUNTS 5.4 S ..........................20 YSTEM ETTINGS 5.4.1 Software Update ........................... 21 6-MAINTENANCE ............................. 22 6.1 A ........................... 22 NNUAL INSPECTION 6.2 C ............................

  • Page 5: 1-Introduction

    RWD Life Science Co., Ltd. is endeavored to improve the product function and service quality. RWD reserves the right to change or alter the contents of user manual without prior notice. If you would like the latest product information, you are welcome to call us or visit our website (http://www.rwdstco.com/).

  • Page 6: Product Features

    Temperature: -20℃~60℃ Storage environment Humidity: 10%~93% (non-condensing) Altitude:＜2000m Use the supplied RWD AC adapter Input voltage: 90 V~264 V, 50/60 Hz, 2 A Working power supply Output voltage: 24V DC; output currency: 1.5 A Voltage and currency fluctuations should be within 5% of the working voltage.

  • Page 7: User Setting Parameter

    1.7 User setting Parameter Parameter Description 4~60 μm Cell size Cell circularity 1~100 Cell brightness 1~255 Bright field luminance 0~100 Magnification (%) 100~400 Focus 0~2,400 Profile options Up to 18 Profiles 1.8 Product List Configuration Items Quantity Description Standard Automated Cell Counter Cell count Standard AC adapter...

  • Page 8: 2- System Safety

    Never use any transmitters such as mobile phones near the instrument. In case the instrument breaks down or requires servicing, shut down the instrument and contact RWD after-sales service. Radiation hazard Always follow all applicable radiation safety procedures when handling radioactive samples. Make sure to take appropriate disinfection and safety measures when disposing of radioactive pollutants.

  • Page 9: Chemical Safety

    allowed to operate within the scope of application, and only the components and accessories suitable for the instrument can be used. The manufacturer will not be held liable for any damage to the instrument caused by any improper operation, including damage to the third party.

  • Page 10: Chemical Waste Safety

    2.2.3 Chemical waste safety Caution! Hazardous waste. Please refer to the SDS and relevant local regulations when handling and disposing of waste. To minimize the risk of chemical waste, you must: a) Read and understand the SDS provided by the chemical manufacturer in the waste container before storing, handling or disposing of chemical waste.

  • Page 11: 3-Product Structure

    3-Product structure The C100-SE Automated Cell Counter is composed of the cell counter host and cell count slide.  Automated Cell Counter Fig. 3-1 Fig. 3-2 ① Touch screen display: 7 inch capacitive screen. ② USB port 1: the count data and image can be downloaded onto an external device with the USB port for storage.

  • Page 12: 4-Preparation

    4-Preparation 4.1 Start-up When the instrument is properly wired, the cell counter can be started. Press the power switch to start the instrument and enter the main interface as shown in the picture below. Fig. 4-1 The main interface contains four parts: System Settings, Previous Counts, Profiles, and Count. Note: If directly inserts the count slide while in this interface, the system will automatically enter the Count page, on which operator can start count immediately according to the default settings and get the count results.
  • Page 13 Fig. 4-2 Click [Trypan Blue] to switch to the Trypan Blue mode. In this mode, the total cell and dead/ live cell concentrations, histograms of dead / live cell size distribution and average size are available for view. Fig. 4-3...

  • Page 14: Adjusting Parameter

    4.2.1 Adjusting parameter Size, brightness and circularity sliders Size: Drag the slider to cause the system to screen the cells according to their size while counting. = Larger target = Smaller target Size screening: the device default range of 7-60 μm, the lower limit of identification can be up to 4 μm;...
  • Page 15 Fig. 4-4 Quick Mode By checking [Quick Mode], you will perform count in a quick mode; otherwise, you will perform count in a standard mode. After selecting the count Profiles in the Quick mode, while the count slide is recognized, the device will calculate the cells and output the results; in the normal mode, the focusing results will be presented first, and then the count results will be output after manually clicks the [Count] button.

  • Page 16: Load Profiles

    4.3 Load Profiles Fig. 4-5 In the left column of the Profiles page, you can delete/ add/ rename Profile protocols, corresponding to icons respectively. Here, in addition to the Default and Red Blood Cells profiles, up to 16 profiles can be added, which can be called directly before and during count. Each Profile protocol defines the count Profiles (size, brightness, circularity) to simplify the workflow.

  • Page 17: Loading Samples Into Slide

     The trypan blue stain was centrifuged to obtain the supernatant;  The cells were centrifuged and then re-suspended. 4.5 Loading Samples into Slide 1) Mix 10 µL of the cell suspension with 10 µL of 0.4% Trypan Blue Stain to prepare the sample. Pipette up and down repeatedly to mix well.

  • Page 18: 5-Cell Count And Cell Survival Rate Assays

    5-Cell count and cell survival rate assays If the system recognizes insertion of the count slide, the Count page will pop up to show the cell view. This page allows you to perform such actions as magnification, focused fine-tuning and light source brightness adjustment, so as to capture optimized images for accurate count of cell concentration or dead/ live cells.
  • Page 19 entering the imaging interface. The overexposed, moderate and underexposed effects of the bright field are shown in Figure 5-2 (A~C) below Fig. 5-2...

  • Page 20: Focusing

    A-overexposure: the light source is too bright, and the background is white, and the cell details are covered by light, which affects cell recognition. B-underexposure: the light source is too low, and the background is too dark, and the cells as a whole are too dark, which affects the judgment of the live and the dead.
  • Page 21 misjudgment of live cells as dead cells by the device.  The focal length of the device was deviated, and the cells presented a fuzzy halo phenomenon, which caused the device to misjudge the dead cells as living cells. How to determine if the focus is correct: ...
  • Page 22 Fig. 5-5 Click to mark cells, as shown in the figure below. Fig. 5-6 Click to enter the Adjustment page to re-adjust the Profiles. Click to enter the Dilution Calculator page, as shown in the figure below, where you can calculate the volume of existing cell stock solution and the volume of buffer solution to be mixed for diluting to the target volume and concentration.

  • Page 23: Saving Results

    is a “Help” symbol, click it to get more about calculation method. Fig. 5-7 5.2 Saving Results After count, you can choose to save/ export the original image, cell marking image, count Profiles, cell count report and other data, and also Profile the default naming rules for results exporting. Click [Save as] to save the results to the local computer in various forms or/ and to an external storage device that meets the requirements.

  • Page 24: Previous Counts

    5.3 Previous Counts Click [Previous Counts] on the Home page to enter the following interface, where you can view the operation Previous data of the instrument, delete and export data to the external storage device. Fig. 5-9 5.4 System Settings Click [System Settings] on the Home page to enter the following interface.

  • Page 25: Software Update

    5.4.1 Software Update The latest version of the software can be updated to the instrument by the USB. The method: First of all, copy the latest software to the USB and insert it into the USB port. When the system successfully identifies the software program in the USB, it will enter the upgrade state as shown below.

  • Page 26: 6-Maintenance

    For details, please contact RWD. It is recommended to clean the instrument regularly to prevent dust buildup from affecting its performance and causing contamination. See the following for cleaning procedures.

  • Page 27: 7-Troubleshooting

    Firmware update fails directory of the USB instead of saving it in a folder or subfolder. The file must be intact with the extension .zip. Motor jammed， please contact the after-sales Turn off the power and contact RWD aftersales personnel personnel!

  • Page 28: 8-Warranty

    If the reworked equipment was found to have been unauthorised disassembly, RWD will not provide after-sales service such as warranty, free maintenance and parts replacement.
  • Page 29 RWD Life Science Web: www.rwdstco.com Add: 850 New Burton Road, Suite 201, Dover, DE 19904, Kent, Delaware, USA Add: 19/20F, Building 9A, Vanke Cloud City III, Liuxin 4 Street, Nanshan District, Shenzhen518000, Guangdong, P.R. China Tel: +001-858-900-6602 +86-755-86111286 After-sales Service: +86-755-86111281...

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