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RWD C100-SE User Manual

Automated cell counter
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C100-SE
Automated Cell Counter
User Manual
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Summary of Contents for RWD C100-SE

  • Page 1 C100-SE Automated Cell Counter User Manual...

  • Page 2: Intellectual Property Right

    RWD’s registered trademark or mark, these trademarks and the related security mark belong to RWD’s intangible property. The use of non RWD’s trademark or mark in this user manual are only for editing purposes, without other purposes, the rights belong to their respective rights owners.

  • Page 3: Table Of Contents

    CONTENTS -INTRODUCTION .............................. 1 ..............................1 VERVIEW ........................1 OMPREHENSIVE ESCRIPTION ............................1 RODUCT EATURES ........................2 NVIRONMENTAL EQUIREMENTS ............................ 2 RODUCT ARAMETER ..........................3 EFINED ARAMETER ..............................3 RODUCT - SYSTEM SAFETY ............................4 ............................4 AFETY YMBOLS ............................ 5 AFETY NFORMATION 2.2.1...
  • Page 4 ........................14 OADING AMPLES INTO LIDE -CELL COUNT AND CELL SURVIVAL RATE ASSAYS ................15 ..........................15 RIGHT IELD SSAYS 5.1.1 Focusing ..............................17 ............................20 ESULTS TORAGE ............................21 REVIOUS OUNTS ............................22 YSTEM ETTING 5.4.1 Software Update ............................22 -MAINTENANCE ..............................

  • Page 5: -Introduction

    RWD Life Science Co., Ltd. is endeavored to improve the product function and service quality. RWD reserves the right to change or alter the contents of user manual without prior notice. If you would like the latest product information, you are welcome to call us or visit our website (http://www.rwdstco.com/).

  • Page 6: Environmental Requirements

    Temperature: -20℃~60℃ Storage environment Humidity: 10%~93% (non-condensing) Altitude:＜2000m Use the supplied RWD AC adapter Input voltage: 90 V~264 V, 50/60 Hz, 2 A Working power supply Output voltage: 24V DC; output currency: 1.5 A Voltage and currency fluctuations should be within 5% of the working voltage.

  • Page 7: Self-Defined Parameter

    1.6 Self-Defined Parameter Parameter Description 4~60 μm Cell size Cell circularity 1~100 Cell brightness 1~255 Bright field luminance 0~100 Magnification (%) 100~400 Focus 0~2,400 Support 17 groups of custom Profiles, 1 group of Profile options system preset Profile 1.7 Product List Configuration Items Description...

  • Page 8: System Safety

    2 - System Safety 2.1 Safety Symbols Flammable environment hazard Never operate the instrument in an environment with flammable gases Electromagnetic interference hazard Make sure to operate the instrument in a controlled electromagnetic environment to avoid any risk associated with instrument failure. Radiation hazard Follow all applicable radiation safety procedures all the time when handling radioactive samples.

  • Page 9: Safety Information

    In case the instrument breaks down or requires any service, shut down the instrument and contact with RWD’s after-sales personnel. 2.2.2 Electrical safety The following provisions should be followed for the safe use of the electrical devices accompanied with the instrument: a) Never disassemble the instrument without any authorization.

  • Page 10: Biosafety

    container. Both containers must be compatible with the chemical properties of the waste materials and comply with local regulations on waste storage.) c) Minimize exposure to chemicals. Wear appropriate protective articles (such as protective glasses, gloves or protective clothing) for chemical handling. For detailed safety guidelines, please refer to SDS.

  • Page 11: -Product Structure

    3 -Product structure  Automated Cell Counter Figure 3-1 Figure 3-2 1：Touch screen display: 7 inch capacitive screen. 2：USB port 1: the count data and image can be downloaded onto an external device with the USB port for storage. USB flash drive format: FAT32. 3：Counting slide port: for inserting the counting slide loaded with samples.
  • Page 12  Cell counting slide The plastic disposable closed counting slide consists of 2 chambers for loading samples, which can be used for loading the same sample as duplicates into 2 chambers for reproducibility validation. Cell count is performed in the center of each chamber. The total volume of cell for count is 20μL, which is equivalent to two small squares in a standard blood cell counter.

  • Page 13: -Preparation

    4 -Preparation 4.1.1 Start-up When the instrument is properly wired, users are allowed to start up the cell counter. Press the power switch to start up the instrument and enter the main interface as shown in the picture below. Figure 4-1 The main interface contains four parts: System Settings, Previous Counts, Profiles, and Count.
  • Page 14 Figure 4-2 Click [Trypan Blue] (TP) to switch to Trypan Blue mode under which the total cell and dead/ live cell concentrations, histograms of dead / live cell size distribution and average size are available for view. Figure 4-3...

  • Page 15: Adjusting Parameter

    4.2.1 Adjusting parameter Size, brightness and circularity sliders Figure 4-4 Size: drag the slider to enable the system to screen the cells by the size while counting. = Larger size = Smaller size Size screening: allowable range of 7-60μm by default, the lower limit of identification up to 4μm.
  • Page 16 adjust the circularity threshold. Click the [help] symbol in the upper right corner of the interface to learn more about the concepts of size, brightness, circularity, and so on. Figure 4-5 Quick Mode By checking [Quick Mode], you will perform count in a quick mode; otherwise, you will perform count in a standard mode.

  • Page 17: Load Profiles

    Restore Default Click [Default] to restore part of the initial settings, including Auto Exposure, Quick Mode, Standard Mode, threshold value of each Profiles, etc. 4.3 Load Profiles Figure 4-6 In the left column of the Profiles interface, you can delete/ add/ rename/ Lock profiles by clicking the icons of respectively.

  • Page 18: Sample Preparation

    4.4 Sample Preparation For best results, the instructions below should be followed: 1) Make sure the cell suspension is well mixed before taking the cell sample. After the cell sample is left standing for a long time, it is necessary to flick the tube or gently blow the cell suspension and draw the sample from the center of the tube rather than the bottom of the tube.

  • Page 19: -Cell Count And Cell Survival Rate Assays

    5 -Cell count and cell survival rate assays If the system recognizes insertion of the counting slide, the file name editing interface will pop up. Shown as figure 5-1. Figure 5-1 Enter the file name and click [Enter] or click [skip] (“skip” means that the default name or last typed name is selected) to enter the count interface.
  • Page 20 For setting the dilution factor of the sample. Click “1×” to pop up a numeric keypad. The range of setting value: 1.00~999.00. Note: The default dilution of Trypan Blue mode is “2×”, and the default dilution of "AO+PI" mode is “2×”. Dilution factor of sample: The cell concentration counted by cell counter is the actual concentration of the cells on the current counting slide, which may be obtained by dilution of the original solution, so you need to convert...

  • Page 21: Focusing

    Figure 5-3 A Overexposure: the light source is too bright, and the background is white, and the cell details are covered by light, which affects cell recognition. B Underexposure: the light source is too low, and the background is too dark, and the cells as a whole are too dark, which affects the judgment of the live and the dead.
  • Page 22 showed above the bar. Adjustment range of aggregation value: 0-2400. The mean of [Set]: customize the focus value. The next time the instrument is used, the system will focus based on the focus value of the last “Set” in order to quickly obtain good pictures for accurate counting.
  • Page 23 the changes of cell imaging in a clear manner when the focal length changes and select the state with the clearest cell edge as the focus. By clicking , the slide regulator will show up to allow users to zoom in/out the active view/ image. Upon completion of operations on the [Count] interface, the [Result] interface will pop up, where users are allowed to zoom in, mark cells, or view histograms, adjust profiles and count for the captured images.

  • Page 24: Results Storage

    Figure 5-7 Click to enter the Adjustment interface to re-adjust the Profiles. Click to enter the Dilution Calculator interface, as shown in the figure below, where users are allowed to calculate the volume of existing cell stock solution and the volume of buffer solution to be mixed and diluted to the target volume and concentration.

  • Page 25: Previous Counts

    name. Note: up to 1000 entries can be stored in the system. If you continue to save new data beyond this limit, the count results saved at the earliest time will be deleted by default. Note: Do not remove the USB flash drive when data is being exported. Otherwise, the USB flash drive will be damaged and data will be lost.

  • Page 26: System Setting

    5.4 System Setting Click [System Setting] on the main interfae to enter the following interface. It allows you for performing such actions as software update, date / time setting, language shift. Note: “Maintenance” is only for after-sales personnel. Figure 5-11 5.4.1 Software Update The latest version of the software is available for updating to the instrument by the USB flash drive.
  • Page 27 Figure 5-12...

  • Page 28: -Maintenance

    6 -Maintenance 6.1 Cleaning  Cleaning the touch screen 1) Gently wipe off the touch screen with a soft lint free cloth moistened with an LCD cleansing detergent. Be gentle and cautious during cleaning. Wipe the touch screen dry immediately after cleaning. 2) Prevent ingress of cleaning solvent into the Power button, power socket, counting slide port or USB port.

  • Page 29: -Failure/Alarm

    The package must be uncorrupted during transmission. Alarm prompt “Motor jammed, please Power off the device and contact RWD after- contact the after-sales personnel!” sales personnel. Alarm prompt “Camera error, please Power off the device and contact RWD after- contact after-sales personnel!”...

  • Page 30: -Warranty

    During the warranty period, the equipment cannot be used normally due to problems such as materials and process defects. RWD undertakes after-sales service such as equipment maintenance and parts replacement.
  • Page 31 RWD Life Science Web: www.rwdstco.com Add: 850 New Burton Road, Suite 201, Dover, DE 19904, Kent, Delaware, USA Add: 19F, Building 9A, Vanke Cloud City III, Liuxin 4 Street, Nanshan District, Shenzhen518000, Guangdong, P.R. China Tel: +001-858-900-6602 +86-755-86111286 After-sales Service: +86-755-86111281...

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