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RWD MP-1000 User Manual

Micropipette puller
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MP-1000
Micropipette Puller
User Manual
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Summary of Contents for RWD MP-1000

  • Page 1 MP-1000 Micropipette Puller User Manual...
  • Page 2 RWD’s registered trademark or mark, these trademarks and the related security mark belong to RWD’s intangible property. The use of non RWD’s trademark or mark in this instruction manual are only for editing purposes, without other purposes, the rights belong to their respective rights owners.
  • Page 3 CONTENTS -INTRODUCTION ..........................1 ............................1 VERVIEW ............................1 AFETY ..........................1 NTRODUCTION ............................2 EATURES ........................... 2 ARAMETERS ..........................3 RODUCT LIST ......................... 3 ONDITIONS -IMPORTANT INFORMATION AND SECURITY ................4 ............................4 YMBOLS ........................... 4 AFETY AUTIONS General safety ..........................4 Safety Instructions ........................
  • Page 4 RWD Life Science Co., Ltd. is committed to continuously improving product functions and service quality, and reserves the right to make changes to any product described in this manual and the content of this manual without prior notice.
  • Page 5 Preparation of liquid flow pipeline and liquid flow control system in microfluidic chip technology, preparation of nozzles in new printed Application in industry circuit and 3D printing technology, preparation of operating needles in cell cutting and capture systems, probe production or preparation of particle capture needles in material science.
  • Page 6 Product list Configuration Name Quantity Description Standard MP-1000 Micropipette Puller Main unit Plug-in power wire for device power Standard Power wire input Standard Electrode storage box For storing microelectrodes pulled disassemble assemble Standard Double-end screwdriver accessories. One end is cross and the other slotted.
  • Page 7 The device is only allowed to operate within its applicable scope, and only accessories suitable for this device are allowed to be used. RWD shall not be responsible for any device damage caused by any non-conforming operation, including the damage to a third party.
  • Page 8 -Product interface and buttons ② ① ③ ④ ⑤ ⑥ Figure 3-1 ⑦ ⑧ Figure 3-2 No. Components Quantity Description ① Install the filament ② Drierite canister interface Connect the Drierite canister ③ Capillary glass mounting brackets Install the capillary glass ④...
  • Page 9 -Operating Procedures Initial start Before using the device for the first time, or after replacing the heating filament, pay attention to check and ensure that the heating filament is tightened! This device should be placed on a stable and level platform during use. Do not place on the unstable platform! Connect the power wire, plug it into the three-hole socket, press the power switch to start the device, and the system first displays the boot interface.
  • Page 10 Pressure (1-999) The range of air pressure to cool glass and the heating filament is 1-999 The reasonable range of duration of the humidity control chamber being filled Puff(s) with dry gas at the beginning and the end of the pulling is 5-20 seconds In order to avoid unstable pulling or burning the heating filament, the heat cannot Safe heat be lower or higher than 10% of the softening point...
  • Page 11 Do not use excessive force when pulling the bars and tightening the knob, otherwise the glass may be broken! Note! When the microelectrode used in patch clamp, RWD recommends the model of glass is B-15086-10F, B-12069-10F, B-150110-10F, B-12094-10F! Softening point Test The heating assembly of this device increase the temperature of the heating filament via the current to soften the glass.
  • Page 12 Figure 4-5 Get and record the softening point test value. Figure 4-6 Click on the interface in Figure 4-6 to enter the [Softening point test] interface as shown in Figure 4-4 and retest the softening point. Click to enter the [Main Interface] shown in Figure 4-2, and the current softening point test value will be automatically filled in the softening point input box.
  • Page 13 In the interface shown in Figure 4-7, each parameter can be edited. Figure 4-7 Pull Protocol On the [Main Interface], click to select the preset protocols of RWD for pulling. Select the heating filament model. Heating filaments are all box-shaped. Model Specification(box length× width) HF-3025B 3.0×...
  • Page 14 Select capillary glass. Model Specification(O.D.× I.D.× length) B-15086-10F 1.5× 0.86× 100 mm B-10050-10F 1.0× 0.5× 100 mm B-10078-10F 1.0× 0.78× 100 mm B-12069-10F 1.2× 0.69× 100 mm B-12094-10F 1.2× 0.94× 100 mm B-150110-10F 1.5× 1.1× 100 mm Figure 4-9 Select the application of microelectrodes. Figure 4-10 Finally, the system generates program parameters based on the above three options, and the current protocol can be used as a new program or overwrite current parameters.
  • Page 15 Figure 4-11 Start and pause The green button [START/STOP] is a multi-function button, which can start or pause pulling; the red button [RESET] can restart the system. Program List Click on the [Main Interface] as shown in Figure 4-2 to save to the current program or save as a new program.
  • Page 16 Remove microelectrode After pulling, hold the end of the microelectrode with one hand, loosen the knob with the other hand, and take out the microelectrode on both sides from the top of the V-groove. Note! The temperature of the newly pulled microelectrode is very high. Please do not approach the heating chamber! When removing the microelectrode, do not slide along the groove, otherwise the tip of the microelectrode may be hit and damaged.
  • Page 17 Warning! Test the softening point before turning on the jaw preheating funciton, otherwise the preheating time may be overlong or the heating filament may be burned! Turn on the "Preheat jaws to 70℃" function. It may be necessary to adjust the recommended parameters to obtain the target microelectrode.
  • Page 18 When testing the median velocity, the recommended cycles are 4 and 5! Recommended parameter Velocity 24 25 26 27 28 29 31 32 33 34 35 36 Cycle Median velocity Select velocity Test the impedance corresponding to the median velocity of 4 cycles and 5 cycles, and compares it with the target impedance, and selects the median velocity which the impedance is closer to the target impedance for adjustment.
  • Page 19  Multi-line adjustment method Use multi-line adjustment method if the target microelectrode cannot be pulled by the single-line adjustment or the cycle value changes after the single-line adjustment. The heat adjustment range is ± 30, and the velocity adjustment range is ± 10. Example: Select the velocity of 30 to cycle for 5 times, divide the setting parameters into 5 lines, and then adjust the heat or velocity of last 1-3 lines to obtain the parameters of the target microelectrode.
  • Page 20 The tip of the microelectrode becomes smaller, the taper gets decrease longer, and the impedance becomes larger The tip of the microelectrode becomes larger, the taper gets increase shorter, and the impedance becomes smaller Delay 10 units change The tip of the microelectrode becomes smaller, the taper gets decrease longer, and the impedance becomes larger The tip of the microelectrode becomes smaller, the taper gets...
  • Page 21 System diagnosis Pressure diagnosis Pressure diagnosis is to check whether the air pump runs, whether the air valve is normally opened and closed, and whether there is air leakage. Figure 4-20 Set pressure, the value range is 1-999, turn on [Automatic pressure], the air circuit will automatically run to the set pressure;...
  • Page 22 Heat diagnosis Figure 4-22 Pull diagnosis Set the pull value within the value range of 0-255, pull the puller bar handles to the middle, and press the green [START] button on the right side of the display. Set the pull value to correspond to the pull during pulling, and the user can judge whether the pull diagnosis is normal.
  • Page 23 Figure 4-24 Help About this puller Figure 4-25 Track leakage Click and operate following the prompts to locate leakages, refer to 4.12.1 System diagnosis. Figure 4-26...
  • Page 24 Install filament Users can check the installation method of heating filaments step by step, or refer to 6.3 Replace filament. Click “Next” to follow the instructions. Figure 4-27 Install glass Users can check the installation method of glass step by step, or refer to 4.2 Install glass. Click “Next”...
  • Page 25 System Maintenance Click to decrease the brightness and click to increase the brightness. [Maintenance] is only for RWD after-sales personnel. Figure 4-30...
  • Page 26 -Troubleshooting Alarms/Faults Solutions Wait for the system to be cooled down to Electric circuit overheated. 70 ℃; Wait for cooling down to 70℃. Please contact technical If the alarm is not removed, Please support if the problem persists after sufficient cooling. contact our after-sales personnel Wait for the jaw to be cooled down to 90 ℃;...
  • Page 27 Try to prevent vibration or collision when the device is moved;   When the accessories of the device are damaged, please contact RWD after-sales service for inspection and maintenance. The device shall not be disassembled by unauthorized personnel to avoid injury to the operator or damage to the device.
  • Page 28 Unscrew the two screws on the jaw, as shown in Figure 6-2, and remove the old heating filament. Figure 6-2 First check whether the jaw groove corresponds to the size of the new filament, as shown in Figure 6-3. If the size corresponds, install the new filament directly on the jaw. If the size does not correspond, unscrew the screw ①...
  • Page 29 Figure 6-5 Install the new heating filament on the jaw to make the glass go through the center of the filament, and put in two screws①②. Be careful not to tighten the screws at this time, as shown in Figure 6-6. Figure 6-6 Note! To prevent potential damage, do not pinch the filament or touch it with sharp objects such as...
  • Page 30 Figure 6-8 10) Hold the jaw, loosen screws ① and ②, and adjust both jaws up and down as shown in Figure 6-9, until the glass is vertically centered inside the filament. When loosening screws ① and ② , hold this jaw to prevent the filament from damage! Figure 6-9...
  • Page 31 Figure 6-11 Figure 6-12 13) Turn on the power switch, pull the glass to verify the installation of the heating filament, and refer to 4.3 Softening point Test——4.9 Pull result. Warning! Do make sure to adjust the filament and test the softening point after replacing a new heating filament! 14) Align the tips of the left and right micropipettes side by side and compare their lengths from tip to shoulder.
  • Page 32 Figure 6-15 Figure 6-16 Figure 6-17 Replace Drierite canister When the drierite is dry, it is blue or blue-purple, and becomes pink after absorbing moisture. When two thirds of the drierite is pink, replace it with a new drierite canister. Unplug the power wire before replacing the drierite canister.
  • Page 33 Confirm whether the touch screen operation is normal;   Confirm whether the identification is firmly pasted; If it is found that some parts or materials need to be replaced during general check or maintenance,  please contact RWD after-sales service for support.
  • Page 34 -Warranty The warranty for this system starts from the day when it leaves the factory. RWD provides after-sales support such as device maintenance and parts replacement in case of the device failure due to defects in materials or process during the warranty period.
  • Page 35 RWD Life Science Co., Ltd. Add: 10410 Corporate Drive, Sugar Land, TX 77478, USA Add: (Floor 9, 19&20 Building 7A, Floor 9 Building 7D) Room 1901, Building 7A, International Innovation Valley, Dashi 1st Road, Xili Community, Nanshan District, Shenzhen 518000, Guangdong, P. R. China Web: www.rwdstco.com...