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RWD 71001-S User Manual

Automated stereotaxic instrument
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Automated
Stereotaxic Instrument
User Manual
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Summary of Contents for RWD 71001-S

  • Page 1 Automated Stereotaxic Instrument User Manual...

  • Page 2: Intellectual Property Right

    RWD’s registered trademark or mark, these trademarks and the related security mark belong to RWD’s intangible property. The use of non RWD’s trademark or mark in this instruction manual are only for editing purposes, without other purposes, the rights belong to their respective rights owners.

  • Page 3: Table Of Contents

    Contents 1 - About ........................... 1 1.1 Product Description ......................1 1.2 General Description ......................1 1.3 Features ........................... 1 1.4 Product Applications....................... 1 1.5 Environmental Requirements ..................2 1.6 Product Parameters ......................2 1.7 Model Configuration ...................... 3 1.8 Product Option List ......................4 2 - System Security .........................

  • Page 4: About

    RWD Life Science Co., Ltd. is committed to continuously improving product functions and service quality. RWD reserves the right to make changes to any product described in this manual and the content of this manual without prior notice.

  • Page 5: Environmental Requirements

    1.5 Environmental Requirements Please prepare the equipment operating environment according to the conditions listed in the table below to ensure the operability and safety of the system. Temperature: 5°C ~ 40°C Humidity: 15% ~ 95% (non-condensing) Working Environment Altitude< 2000 m Temperature: -20°C ~ 60°C Humidity: 10% ~ 95% (non-condensing) Storage Environment...

  • Page 6: Model Configuration

    Note: Due to the differences between different versions of the user manual, this product list is for reference only. Please check the delivered parts against the enclosed packing list upon receipt and contact your RWD service immediately if any discrepancies are identified.

  • Page 7: Product Option List

    1.8 Product Option List Configuration Unit Model Remark Upgrade Includes manipulator, micromanipulator, control box, Manipulator Module extension interface and electrode cable Includes injection unit + injection unit connection cable + 3.5-inch micropipette+ 7-inch micropipette+ universal Injection Module 71000-I adaptor for all nanojects + 68207 V attachment + filling needle + sealing material + hexagonal wrench.

  • Page 8: System Security

     Users shall be responsible for any fault caused by their improper operations during cleaning, maintenance, or use of the device.  If the device body is disassembled or assembled without approval from RWD, RWD will no longer provide any quality assurance or technical maintenance service commitments for the device.

  • Page 9: System Structure

    3 - System Structure Figure 3-1 Part/Interface Name Function Manipulator It can move and hold accessories. (contains DV-axis, ML-axis) AP-axis Secure the manipulator. U-shaped Base Secure the animal adaptor and ear bars. Control manipulator and injection, etc., set parameters and Auto-Stereotaxic Software control program operation, compatible with Win10/Win11 operating systems.
  • Page 10 Part/Interface Name Function and it must be connected to the matching control box of the manipulator to work. The mechanical master control box is programmed to control Control Box the manipulator and injection and to control the operation and data exchange of different components. Stops the control of the manipulator by the micromanipulator Emergency Stop Button box in an emergency.

  • Page 11: System Connection

    1) After unpacking, remove the protective foam and take out all accessories. 2) Please check the materials received against the enclosed packing list. If you find any missing or damaged, please contact RWD after-sales service immediately. 3) Take out the instrument with caution and place it on a flat, level table.
  • Page 12 6) As shown in Figure 4-3, the V-shaped connector can be fixed on the manipulator through knob 6; ① Allen Screw Knob 6 Figure 4-3 7) The lower left figure shows the general fixing accessories. Knob 7 is used to fix and adjust the injection unit.
  • Page 13 Note: The size of the holes on both sides of the sealing post are different, so pay attention to the direction when installing. a) Use a micropipette puller (RWD MP-500 micropipette puller is recommended) to draw micropipette. b) Backfill the pulled micropipette with mineral oil (R480-MO) using a filler needle. For some operation experiments, it is also possible to directly fill the micropipette with injection liquid or directly withdraw the injection liquid without filling mineral oil.
  • Page 14 Figure 4-7 d) Slightly loosen the collet, as shown below. Figure 4-8 e) Put the pulled and filled micropipette into the exposed steel needle piston and slowly insert it into the bottom. Operate gently and slowly to avoid the entry of air bubbles, as shown below.

  • Page 15: System Connection

    4.2 System Connection Refer to the simple connection cable diagram for connection, as shown below. External Devices : Square Port Connection Cable : Micro Cable : BNC Cable : Injection Unit Connection Cable : 7-core Connection Cable : Power Adaptor : Ground Wire Figure 4-10 Simple Connection Cable Diagram 1) Connect the manipulators ML, AP and DV to the corresponding motor ports (ML, AP, DV)
  • Page 16 2) When used with the injection unit, use the injection unit connection cable to connect the fixed injection unit (see Figure 4-15) to the injection port on the control box corresponding to the manipulator connection; finally, connect the control box (see Figure 4-16) to the computer via the square port connection cable, and then start the computer software in order to operate the computer/micro-operation controller via manipulator and injection unit.

  • Page 17: Operation Instructions

    5 - Operation Instructions 5.1 Software Function Introduction This is the PC-based operating software used with the automated stereotaxic instrument. The software integrates the brain atlas and motion control program as well as the syringe pump control program, which allows the user to achieve the motor motion for the AP/ML/DV-axis of the stereotaxic instrument and to control the syringe pump on the software interface.

  • Page 18: Left And Right Manipulator Switching Region

    5.3 Left and Right Manipulator Switching Region It is used to switch between the left and right manipulator operation interfaces, as shown below. Note: When the left/right manipulator control box is not connected, you cannot switch to the operation interface of that manipulator. Figure 5-2 5.4 Origin Setting Region Figure 5-3...

  • Page 19: Fixed-Ponit Displacement Region

    Button Function Hot Keys Click to switch between the set step values. None Click to move forward one step by pressing the AP step value. Keypad " " Click to move backward one step by pressing the AP step value. Keypad "...

  • Page 20: Atlas Display Region

    Note: Manipulator and atlas coordinates cannot be set at the same time! The coordinates cannot exceed the range of motor travel! *Entry point: at the position above the target coordinate along the DV-axis higher than the retraction distance of the skull surface (see the figure below). Entry Point Skull Window Skull Surface...

  • Page 21: Menu/Program Setting Region

    Key functions. Button Click to select [3D View], press the left mouse button and drag in the brain atlas to rotate the brain atlas display in 3D, press the right mouse button and drag in the brain atlas to zoom in/out the image display, press the mouse wheel and drag in the brain atlas to adjust the view position.
  • Page 22 5.8.1.1 Atlas Selection Click to select rat/mouse brain atlas. The brain atlas version is the second edition for mice, the fifth edition for mice and the sixth edition for rats. After selecting the version, click [OK] on the software interface, [Atlas Display Region] will display the corresponding version of the brain atlas.
  • Page 23 Frame Calibration Function If there is a deviation between the AP/ML/DV value displayed in the [coordinate displacement region] of the manipulator on the software interface and the actual reading of AP/ML/DV on the manipulator of the stereotaxic instrument (e.g., offset after long-term use), you can calibrate the AP/ML/DV Coordinate Calibration value and probe position displayed on the software interface here.
  • Page 24 Button Function After clicking, the probe will automatically move to the coordinate position of the currently selected coordinate according to the speed set by the system, the button will become the stop button “ ”, and other interface operations will be disabled during the movement until the movement is automatically completed or the stop button is clicked.
  • Page 25 (such as the auditory cortex), it is necessary to tilt the skull of experimental animals to achieve stereotaxic injection in the target atlas. It can be used with RWD's rat rotation adaptor (68063), mouse rotation adaptor (68065), or rotation stereotaxic instrument.
  • Page 26 5.8.1.6 Atlas Calibration Figure 5-14 [Atlas Calibration] is a process of determing the coordinates of Bregma, Lambda, center point left site, and center point right site, and aligning the animal’s skull size and tilt angle in relation to the coronal, sagittal, and horizontal planes to the brain atlas. You can level the skull according to the atlas angles in relation to the coronal, sagittal, and horizontal planes.
  • Page 27 5.8.1.7 System Settings Set AP/ML/DV Movement Speed Set Retraction Distance Switch System Language Anti-backlash Function On/Off Motional Collision Prompt On/Off Manipulator Collision Prompt On/Off Figure 5-15 Parameter settings: Parameter Setting range Defaults Select to set the movement speed of the manipulator system. Setting range (optional): AP/ML-axis speed (2.00 mm/s, 1.00 mm/s, AP/ML/DV-Axis 0.50 mm/s, 0.20 mm/s, 0.10 mm/s);...

  • Page 28: Program

    5.8.2 Program The program column contains four items: Skull Window Program, Tissue Removal Program, Multi-point Program, and Injection. 5.8.2.1 Skull Window Program [Skull Window Program] precisely move the microdrill fixed on the manipulator, a round/square cranial window of the target size can be precisely drilled into the skull of the animal for subsequent experiments.
  • Page 29 Key functions: Function Button Click to run the skull window program. Click to pause the skull window program. Click to end the skull window program. 5.8.2.2 Tissue Removal Program [Tissue Removal Program] allows precise removal of brain tissue and reduces unnecessary brain damage.
  • Page 30 Key functions: Function Button Click to run the tissue removal program. Click to pause the tissue removal program. Click to end the tissue removal program. Clear the contents of the step list (selected state, depth, number of layers). After entering the tissue removal program interface, click the switch button in the lower- left corner of the atlas to switch between the brain atlas display (Figure 5-18) and the tissue removal display (Figure 5-19).
  • Page 31 5.8.2.3 Multi-point Program Program Run Move to Entry Point Add Coordinates Delete Coordinates Call [Store Coordinate] Coordinates Save to [Store Coordinate] Move Up Move Down Settings: Click this to set the type of signal emitted by the multi-point program and the movement trigger method.
  • Page 32 Click the button “ ” to display the movement setting interface, as shown in the following figures. Figure 5-21 Figure 5-22 Mode settings: Settings Type Description No sending signal. Positive Type of Pulse Send a single 5V high-level signal to the outside, with a pulse width of 5ms. Signal Signal Send...
  • Page 33 Settings Type Description after receiving the external rising edge signal/falling edge signal (optional), it automatically moves to the next coordinate. Manual Moves to the next coordinate after manually clicking Set the delay before injection, injection volume, injection rate/injection time, and needle retention time for each coordinate (Figure 5-24) or click [Overlay] Injection to synchronize all coordinates after unified setting, and click [Save] to Mode...
  • Page 34 5.8.2.4 Injection The system has a built-in injection control program (not available if no syringe pump is connected), which can be used to directly operate the syringe pump for injection, filling, retraction and other operations through the software, facilitating the experiments. The filling/emptying/injection time, volume and status are displayed in real time at the bottom of the interface.
  • Page 35 Parameter settings: Mode Parameter Setting range Defaults Empty Speed 200 nl/s Range: 10 ~ 200 nl/s with a step value of 1 nl/s. Empty Volume 1000 nl Range: 0.6 ~ 5280.0 nl, step value 0.1 nl. Manual mode Fill Speed 100 nl/s Range: 10 ~ 200 nl/s with a step value of 1 nl/s.

  • Page 36: Alarm Prompt

    6 - Alarm Prompt Alarm message prompt Alarm cause Troubleshooting 1) The control box is not powered on. 2) The connection cable between Please power on the control box and control box and restart the software. computer is not Reconnect the cable between control connected or not box and computer.

  • Page 37: Product Cleaning And Maintenance

    7 - Troubleshooting This chapter describes the common problems (faults) during the use of this product, possible causes, and suggested troubleshooting methods. Symptom Troubleshooting method Return the unit to the factory for calibration and maintenance Accuracy abnormal in a regular manner. The motor wires are connected incorrectly.

  • Page 38: Unit Cleaning And Maintenance

    7) Disconnect the power cord if the unit is not in use for a long period of time, place it in a suitable storage environment and slightly loosen the collet. 8) When the device parts are damaged, please contact RWD for inspection and maintenance. Non-authorized personnel from RWD are not allowed to disassemble the device to avoid...

  • Page 39: Product Warranty

    If it is found after inspection that the device has been disassembled without the authorization of RWD, RWD does not provide a warranty or free maintenance and parts replacement services. The warranty statement (including its restrictions) is exclusively issued by RWD, and shall...
  • Page 40 RWD Life Science Co., Ltd. Add: 10410 Corporate Drive, Sugar Land, TX 77478, USA Add: (Floor 9, 19&20 Building 7A, Floor 9 Building 7D) Room 1901, Building 7A, International Innovation Valley, Dashi 1st Road, Xili Community, Nanshan District, Shenzhen 518000, Guangdong, P. R. China Web: www.rwdstco.com...

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71000-s71001-d71000-d

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