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Summary of Contents for HTL Discovery Pro
- Page 1 HTL Discovery Pro Single-channel, 8-channel, and 12-channel Pipettors Instruction Manual...
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Page 2: Table Of Contents
Table of Contents 1. Product Description ..............1 2. Packing .....................2 3. Pipettor Design ................2 4. Safety Recommendations ............3 5. Specifications ................4 6. Pipettor Operation ..............6 7. Operating Instructions ............7 8. Aspiration and Dispensing Instructions ......9 9. Checking Pipetting Accuracy Parameters and Pipettor Recalibration ........... -
Page 3: Product Description
1. Product Description The HTL Discovery Pro single-channel pipettor is a volumetric instrument designed to measure and transfer liquids precisely and safely. It can accommodate volumes from 0.1 μL to 1,000 μL depending on the model. HTL Discovery Pro 8-channel and 12-channel pipettors have been designed for microplate filling. The pipettors enable... -
Page 4: Packing
Lubricant (for single-channel pipettor) 3. Pipettor Design Model Identification Pipettor model volume range is printed on the counter overlay. Additionally, it is printed on the tip ejector bar in the multi-channel pipettors. Single- and Multi-channel Pipettors 2 – HTL DISCOVERY PRO PIPETTORS... -
Page 5: Safety Recommendations
A. Pipetting pushbutton B. Volume adjustment knob: Used to set and lock the preset volume. C. Shaft: Made of high quality plastic, ensuring high chemical and mechanical strength. D. Tip ejector: For multi-channel pipettors, the tips are ejected sequentially, which reduces the required force. E. -
Page 6: Specifications
The accuracy and precision were obtained gravimetrically using manufacturer tips performing at least 10 measurements of distilled water at a temperature of 20°C ± 1°C according to EN ISO 8655 standards. 4 – HTL DISCOVERY PRO PIPETTORS... - Page 7 The use of tips from other manufacturers or filter tips may result in incorrect liquid aspiration and require pipettor recalibration. Nominal Permissible Volume Volume Accuracy Volumes Precision Non-filter (µL) (µL) (µL) Tips (µL) Single-channel Pipettors Min. ±40.0 0.06-0.14 ≤12.0 ±12.0 0.176-0.224 ≤6.0 ±2.7 0.973-1.027 ≤1.3 Max. ±1.5 1.97-2.03 ≤0.7 Min. ±4.0 0.48-0.52 ≤2.8...
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Page 8: Pipettor Operation
Volume Setting NOTE: The red calibration switch above the counter should remain in the lower position during operation. Otherwise, any change in volume will result in a loss of calibration of the pipettor. 6 – HTL DISCOVERY PRO PIPETTORS... -
Page 9: Operating Instructions
A. To enable volume selection, set the black adjustment knob to the upper position. B. To set a certain value, turn the knob. The volume is indicated by the counter and its value consists of four digits that should be read from top to bottom. Digits on the bottom drum represent the scale division for the pipettor model. - Page 10 Do not pipette liquids with temperatures above 70°C. NOTE: When pipetting acids or corrosive solutions which emit vapors, it is recommended to disassemble the shaft and rinse the piston and seal with distilled water after finishing the pipetting operation. 8 – HTL DISCOVERY PRO PIPETTORS...
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Page 11: Aspiration And Dispensing Instructions
8. Aspiration and Dispensing Instructions Aspirating Liquid 1. Press the pushbutton to the first positive stop. Holding the pipettor vertically, immerse the pipet tip into the sample liquid. The depth to which the pipet tip is immersed in the sample liquid depends on the model (for recommended values see Section 7). If the pipet tip is not immersed to the recommended depth or if the pipetting pushbutton is rapidly released, air may enter the disposable pipet tip. -
Page 12: Checking Pipetting Accuracy Parameters And Pipettor Recalibration
(liquids collected), and other conditions (load intensity and sterilization in the autoclave, replacement of components) in which the pipettor is used. If during pipettor operation you find that the accuracy error (the difference between the real aspirated volume 10 – HTL DISCOVERY PRO PIPETTORS... - Page 13 and the preset volume) exceeds the permissible value given in the table in Section 5, the pipettor recalibration procedure should be carried out. Recalibration of the pipettor consists in setting on the counter the value of the liquid volume obtained in weighing. Recalibration of the pipettor is performed only for one liquid volume drawn by the pipettor.
- Page 14 6. Set the calibration switch in the operating position. “Operating” position 7. Fit the ejector pushbutton cap. 8. Repeat the pipetting accuracy check (Section 9: Checking the Pipetting Accuracy Parameter). If the accuracy error value is exceeded, repeat the calibration process. 12 – HTL DISCOVERY PRO PIPETTORS...
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Page 15: Pipettor Maintenance
10. Pipettor Maintenance Depending on the applications and the intensity of use, the pipettor requires periodic maintenance. The components most exposed to vapors of aggressive solutions, such as shaft elements, should be regularly checked and cleaned. To properly carry out the maintenance, familiarize yourself with the design of the shaft holder set shown in Figures 10A, 10B, 10C, and follow the instructions given in this Section. - Page 16 *Seal (J) used only in models 2 and 10 μL 14 – HTL DISCOVERY PRO PIPETTORS...
- Page 17 Single-channel Pipettors 0.1-2 µL, 0.5-10 µL, 2-20 µL, 10-100 µL (Figure 10A) In order to check, clean, or replace the shaft (C) or sealing parts (J, I), it is not necessary to dismantle the entire shaft assembly. To gain access to these parts, hold the nut of the shaft holder (F) and unscrew the tip ejector (D).
- Page 18 16 – HTL DISCOVERY PRO PIPETTORS...
- Page 19 Single-channel Pipettors 20-200 µL, 100-1,000 µL (Figure 10B) • Unscrew the shaft cap (M). • Remove the plunger elements (G) from the shaft. • Check the condition of components and clean as recommended. • If necessary to replace shaft (C) or tip ejector (D), disconnect the two parts by unscrewing the tip ejector from the nut.
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Page 20: Troubleshooting
The inside of the pipettor is ones if necessary. contaminated. Apply a small amount of lubricant onto No lubrication on the the plunger and reassemble the set sealing elements. in the correct order. 18 – HTL DISCOVERY PRO PIPETTORS... - Page 21 Problem Cause Solution The inside of the pipettor is contaminated due to aspiration of aggressive Unscrew the shaft substances. set, wash the parts. Uneven Replace the elements work of the with new ones if The inside of the pipettor pipetting set, necessary.
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Page 22: Spare Parts
1,000 SP19650 SP19611 SP19612 SP19613 I, (J) O-ring set (and seal*) SP19614 SP19615 1,000 SP19616 Calibration key SP19674 Shaft cap SP19644 Shelf clip SP19483 *Seal (J) used only in models 2 and 10 μL 20 – HTL DISCOVERY PRO PIPETTORS... -
Page 23: Limited Warranty
Corning HTL of any such defect. Corning HTL is not liable for any incidental or consequential damages, commercial loss or any other damages from the use of this product. - Page 24 Supplier _________________________________________________ Warranty/Disclaimer: Unless otherwise specified, all products are for research use only. Not intended for use in diagnostic or therapeutic procedures. Corning HTL makes no claims regarding the performance of these products for clinical or diagnostic applications. This manual is available in other languages at www.htl.com.pl.
- Page 25 For additional product information or technical information, visit www.htl.com.pl or contact your local sales office. ASIA/PACIFIC Singapore t 65 6572-9740 Australia/New Zealand f 65 6735-2913 t 61 427286832 Taiwan Chinese Mainland t 886 2-2716-0338 t 86 21 3338 4338 f 886 2-2516-7500 f 86 21 3338 4300...
- Page 26 © 2021, 2023 Corning HTL SA. All rights reserved. 9/23 CLSHTL-AN-1004DOC REV2 Corning HTL SA, Daniszewska 4, 03-230 Warsaw www.htl.com.pl Made in Poland For a listing of trademarks, visit www.corning.com/clstrademarks. All other trademarks are the property of their respective owners.
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