Summary of Contents for Hamamatsu Photonics ORCA-Fire C16240-20UP02
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ORCA-Fire Digital CMOS Camera C16240-20UP C16240-20UP01 / C16240-20UP02 Instruction manual Thank you for your purchase Follow the safety precautions in Chapter 1 in order to avoid personal injury and • damage to property when using this camera. The manual describes the correct handling method of the camera and provides instructions that should be followed to avoid accidents.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 1. SAFETY PRECAUTIONS SYMBOLS The symbols shown below are used for this camera. Direct current Alternating current CLASSIFICATION OF WARNINGS We have classified the warnings symbols that appear in this instruction manual and on the camera as follows for your convenience.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 Power supply Use the camera with the indicated voltage on the rating sticker. Using a different voltage can damage the camera and lead to fire or electric shock. Cables Do not to place heavy objects on cables or bend them excessively. Doing so can damage the cables and lead to fire or electric shock.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 AC adapter When unplugging the power supply cord, do not pull on the cord. Remove the plug from the outlet to avoid causing electric shock or fire. When unplugging the power supply cord, do not pull on the cord, but remove the plug from the camera to avoid breakdown of the AC adapter or the camera.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 FCC Rules This device complies with part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) This device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 2. CHECK THE CONTENTS OF PACKAGE When opening the package, check that the following items are included before use. If the contents are incorrect, insufficient or damaged in any way, contact a Hamamatsu subsidiary or your local distributor before attempting to operate the camera.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 3. INSTALLATION Avoid using or storing this camera in the following places Places where the temperature is not the operating temperature indicated in the specifications • Places where the temperature is not the storage temperature indicated in the specifications •...
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 Contents 1. SAFETY PRECAUTIONS ................... 1 SYMBOLS ........................... 1 CLASSIFICATION OF WARNINGS ..................1 2. CHECK THE CONTENTS OF PACKAGE ............5 3. INSTALLATION ....................6 4. OVERVIEW ......................9 5.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 13. TROUBLESHOOTING ..................58 13-1 IMAGE IS NOT TRANSFERRED ..................58 13-2 ALTHOUGH IMAGES ARE TRANSFFERED ..............58 14. SPECIFICATIONS .................... 59 14-1 CAMERA SPECIFICATIONS .................... 59 14-2 SPECTRAL RESPONSE CHARACTERISTICS (TYP.) ............ 62 15.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 4. OVERVIEW The Digital CMOS camera (C16240-20UP / C16240-20UP01 / C16240-20UP02) utilizes a image sensor (scientific CMOS image sensor: sCMOS) using the latest CMOS design technologies and ultra-small semiconductor technologies and realizes low readout noise. The camera provides 10.5 megapixels resolution at 115 fps (frames/s) while achieving 1.0 electrons (rms) readout noise performance.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 (7) Real-time correction functions There are a few pixels in CMOS image sensor that have brighter or darker intensity, when compared to surrounding pixels. The camera has a real-time variant (defective) pixel correction feature to further improve image quality.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 (9) Camera readout modes The camera has two kinds of readout mode, Area readout mode and Lightsheet readout mode. The camera also has four readout direction in Lightsheet readout mode. (Camera readout modes) (Scan speed) (Readout direction)
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 6. NAME AND FUNCTION OF PARTS (1) C16240-20UP (C-mount type) Figure 6-1 (2) C16240-20UP01 (F-mount type) Figure 6-2...
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 (3) C16240-20UP02 (M36-mount type) Figure 6-3 Place the camera the ventilation ports to be lateral side. Do not place the • rear panel of the camera, which connectors are located, to be at the bottom (Do not block ventilation openings.).
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 CoaXPress interface connector 1 - 4 [CoaXPress 1 - 4] The connector 1 to 4 are connected to the CoaXPress interface connector 1 to 4 on the computer, respectively. • When a connection interface is changed from CoaXPress to USB, and vice versa, the application software must be closed and the camera must be turned off.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 7. CONNECTION Refer to the figure when connecting the various cables. (1) CoaXPress interface Figure 7-1 (2) USB interface Figure 7-2...
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 Place the camera the ventilation ports to be upward. Do not place the rear • panel of the camera, which connectors are located, to be at the bottom (Do not block ventilation openings.). •...
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 8. Base plate • Do this procedure on the stable working space and conditions. • Do not use other screws nor other wrench which were not included. ATTACHMENT OF BASE PLATE 1.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 A hexagonal wrench is perpendicularly inserted to a screw. Fasten screws by hexagonal wrench while holding the camera. 3. Confirm the base plate is firmly fixed. 4. Attach the camera onto tripod or lab jack. REMOVEMENT THE BASE PLATE Remove the base plate in the reverse procedure of 8-1clause.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 9. OPERATIONS OPERATING PRECAUTIONS Be careful of the following when you operate the camera. (1) Cooling method Cooling of this equipment is done using a Peltier element. With a Peltier element, when current is supplied, one surface is cooled, and the other surface is heated.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 PREPARATING FOR IMAGING Use the following procedure when start operating the camera. • When you connect cables, turn off the power supply of the camera and the peripheral devices. • When using F-mount (C16240-20UP01), an F-mount adapter can be subject to light leaks due to the mating flange mechanism.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 STARTUP DCAM CONFIGURATOR The following is a procedure to startup “DCAM Configurator”. 1. Open “Setup.exe” in the DCAM-API software’s folder. If the DCAM-API software is not installed on your computer, insert the media of DCAM-API software in the slot of your computer.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 4. “DCAM Configurator” window is displayed. The startup is completed with this. - Even if the camera's power supply is turned off, the state of setting is kept. - The state of setting can confirm according to “Hardware” icon on DCAM Configurator window. - After the startup, operate DCAM Configurator according to "DCAM Configurator Instruction manual".
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 UPDATE PROCEDURE OF COAXPRESS INTERFACE BOARD If you use an optional CoaXPress interface board, when the driver included in DCAM-API does not match the firmware version of the interface board, the following message prompting update of the interface board may be displayed.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 10. DESCRIPTION OF CMOS IMAGE SENSOR 10-1 THEORY OF CMOS IMAGE SENSOR The pixel of a CMOS image sensor is composed of the photodiode and the amplifier that converts the charge into voltage.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 10-2 READOUT METHOD OF CMOS IMAGE SENSOR The CMOS camera adopts a rolling shutter readout for high speed imaging. In the camera, the odd and even lines are read out in pairs, therefore the exposure and readout timing is slightly delayed as the readout moves down the sensor (Figure 10-2).
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 (3) Over light Be careful not to input too strong light such as high-energy laser into CMOS • image sensor because CMOS image sensor may be damaged by over light.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 11. DESCRIPTION OF VARIOUS FUNCTIONS 11-1 AREA READOUT MODE 11-1-1 CAMERA READOUT MODES (READOUT DIRECTION) The camera reads out in two line pairs simultaneously. Figure 11-1 Readout direction of Area readout mode 11-1-2 READOUT METHODS (1) Full resolution readout mode (1×1 readout) Perform charge readout from camera individually for all pixels.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 11-1-3 READOUT SPEED (SCAN SPEED) The readout speed can achieve a frame rate of 115 fps for full resolution with low noise (1.0 electrons (rms)) . The CoaXPress interface is required to transfer the full resolution image data at the full speed of 115 frames per second.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 11-1-4 FRAME RATE CALCULATION (1) CoaXPress Exp1 = 7.309 µs to 10 s (input in units of seconds) 1H = 7.309 µs Exp2 = ROUNDUP (Exp1 / 1H) * Round up to integer Vn1 = Number of vertical lines Vn2 = (Vn1 / 2) + 5 Conditions...
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 (2) USB Exp1 = 7.309 µs to 10 s (input in units of seconds) 1H = 7.309 µs Exp2 = ROUNDUP (Exp1 / 1H) * Round up to integer Hn1 = Number of horizontal lines (Binning OFF) BN = Binning setting Output bit depth BW = Interface band width...
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 • The frame rate value is valid 3 digits and rounded down to 4th digit. • The calculation formula and the frame rate value of Start trigger mode (External trigger mode) are same as Free running mode.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 11-1-5 CONFIGURING EXPOSURE TIME The exposure time setting can be done by the units of seconds. The actual exposure time setting is defined by the following formula, and the camera automatically calculates a longer and closest value from the specified exposure time setting.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 11-1-6 CAMERA OPERATION MODES 11-1-6-1 Free running mode The camera has Free running mode which the exposure and readout timing can be set and controlled by an internal microprocessor. Free running mode has normal readout mode (in which the exposure time is longer than the 1 frame readout time) and electrical shutter mode (in which the exposure time is shorter than the 1 frame readout time).
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 (2) Electrical shutter The electrical shutter mode is used to get a proper signal level when signal overflow happens due to too much input photons in normal readout mode. In this mode, the fastest frame rate is 115 fps (Via CoaXPress), 15.7 fps (Via USB) at full resolution even when the exposure time is short.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 11-1-6-2 External trigger mode The camera has various external trigger functions to synchronize the camera with the external equipment. In External trigger mode, the external equipment becomes a master and the camera becomes a slave.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 (2) Global reset Edge trigger mode Global reset function enables to reset the electric charge of all pixels at the same time. Then all pixels can start exposure at the same time. With this Global reset Edge trigger mode, the exposure of all pixels begins on the edge (rising / falling) timing of the input trigger signal into the camera.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 (3) Level trigger mode The Level trigger mode is used to control both exposure start timing and exposure time length by inputting external trigger pulses. In this mode, the camera starts exposure at the start of high or low period of the input trigger pulse and stops exposure at the end of high or low period of the input trigger pulse.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 (4) Global reset Level trigger mode Global reset function enables to reset the electric charge of all pixels at the same time. Then all pixels can start exposure at the same time. The example below is for the trigger level High.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 (5) Synchronous readout trigger mode The Synchronous readout trigger mode is used for continuous imaging when it is necessary to control the exposure start timing of each frame from an external source. It is useful for confocal microscopy.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 Trigger Times; Also in the Synchronous readout trigger mode, synchronous readout can be controlled by specifying, the number of timing pulses to determine the exposure time. The input trigger is valid only during the trigger ready is enabled.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 11-1-6-3 Start trigger mode Start trigger mode is to start operating the camera by a trigger input for a continuous imaging. It is useful to secure the frame rate as fast as possible when continuous image acquisition and not to sacrifice the exposure time.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 11-1-7 TIMING OUTPUT The camera provides a range of trigger output signals to synchronize with an external instrument and the camera becomes the master and the external instrument becomes the slave. There are four different trigger output functions as follows.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 External trigger 0(H) 1(H) ・ ・ ・ N-1(H) N(H) Sensor readout Read end Delay Width Vsync Delay Width Trigger Delay Width Figure 11-12 Programmable timing output (4) Trigger ready output The trigger ready output is useful to make the frame intervals as short as possible in external trigger mode.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 11-2 LIGHTSHEET READOUT MODE Lightsheet Readout Mode is a unique feature of CMOS image sensor which provides improved control over the rolling shutter mechanism. By finely synchronizing the camera readout with the illumination scan, scattered light is rejected allowing images of higher signal to noise ratios to be acquired.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 11-2-2 ABOUT READOUT AT LIGHTSHEET READOUT MODE (1) Readout methods This mode can set Sub-array readout. Binning readout mode is not supported. The minimum selectable steps of size and position of sub-array can be configured according to the table below.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 11-2-3 FRAME RATE CALCULATION (1) CoaXPress Exp1 = 7.309 µs to 276.9 ms (input in units of seconds) 1H = 7.309 µs to 233.9 µs Exp2 = ROUNDUP (Exp1 / 1H) * Round up to integer Vn1 = Number of vertical lines Vn2 = (Vn1 / 2) + 5 Operation modes...
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 (2) USB Exp1 = 7.309 µs to 276.9 ms (input in units of seconds) 1H = 7.309 µs to 233.9 µs Exp2 = ROUNDUP (Exp1 / 1H) * Round up to integer Hn1 = Number of horizontal lines Output bit depth BW = Interface band width...
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 11-2-4 READOUT TIME OF THE HORIZONTAL LINE Readout time and exposure time can be varied with Lightsheet Readout Mode for synchronizing the camera readout with the illumination scan. = Number of vertical lines = 7.309 µs to 233.9 µs Readout time = (Vn / 2 + 5) ×...
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 11-2-5 TIMING DIAGRAM (1) Free running mode Internal exposure timing setting 0(H) 1(H) ・ ・ ・ N-1(H) N(H) Sensor readout CoaXPress data output USB data output Any-row exposure timing output 1H readout time : 7.309 μs –...
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 11-2-6 TRIGGER OUTPUT The camera provides a range of trigger output signals to synchronize with an external instrument and the camera becomes the master and the external instrument becomes the slave. There are three different trigger output functions as follows.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 11-3 REAL-TIME DEFECT PIXEL CORRECTION CMOS image sensors have pixels with high dark current caused by the failure of the silicon wafer, which appear as white spots with long exposure time. The camera has real-time pixel correction features to improve image quality.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 11-4 DATA REDUCTION FUNCTIONS The camera provides 10.5 megapixels resolution at 115 fps and 16 bit of gradation. Then the camera outputs 2.4 GB of data per second, and large capacity of storage device would be necessary to store such a large amount of data.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 11-5 MASTER PULSE The camera has master pulse function which can generate pulses that is independent of the exposure or readout timing of image sensor. External trigger mode can work synchronized with the timing pulses that the master pulse generates, except for External trigger mode in Lightsheet Readout Mode.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 12. MAINTENANCE 12-1 CARE Perform cleaning of this equipment with the dry soft cloth. • Do not wipe with a damp cloth or unclean cloth. Then, the glass window on the image sensor should be cleaned according to the following. 1.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 13. TROUBLESHOOTING If an abnormality occurs, look up the possible causes in the following tables and, if necessary, report the details to Hamamatsu subsidiary or your local distributor. 13-1 IMAGE IS NOT TRANSFERRED Cause Measures Chapter...
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 14. SPECIFICATIONS 14-1 CAMERA SPECIFICATIONS (1) Image quality specifications Imaging device sCMOS image sensor Effective number of pixels 4432 (H) × 2368 (V) Pixel size 4.6 μm × 4.6 µm Effective area 20.387 mm ×...
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 (2) Functional specifications Sensor mode Area readout / Lightsheet readout Readout mode Full resolution readout / Binning readout * / Sub-array readout * Exposure time 7.309 µs ~ 10 s (7.309 µs step) Readout time * 3, 4 8.695 ms...
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 1 Digital binning, 2×2, 4×4. At Area readout mode. 2 Minimum settable step of the size and position are as follows: It is in only the case that the camera is used with DCAM-API. Horizontal size Vertical size Horizontal position...
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 (7) Applicable standards EN61326-1: 2013 Class A Emission limits: CISPR 11 Group1 Class A Immunity requirements: Table2 Judgment criteria A Intensity fluctuation of image is within ±5 %. Though the operating function is temporarily Function performance impaired, Judgment criteria B...
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 15. DIMENSIONAL OUTLINES 15-1 C16240-20UP (Unit: mm)
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 15-2 C16240-20UP01 (Unit: mm)
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 15-3 C16240-20UP02 (Unit: mm) 15-4 BASE PLATE (Unit: mm)
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 16. WARRANTY Hamamatsu Photonics have fully inspected this camera and checked that its performance conforms to specifications. In the unlikely event of a breakdown or other malfunction, contact a Hamamatsu subsidiary or your local distributor.
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Digital CMOS Camera C16240-20UP / C16240-20UP01 / C16240-20UP02 Instruction manual_Ver.1.5 17. CONTACT INFORMATION Manufacturer HAMAMATSU PHOTONICS K. K., Systems Division 812 Joko-cho, Chuo-ku, Hamamatsu City, Shizuoka Pref., 431-3196, Japan Telephone (81) 53-431-0124, Fax: (81) 53-435-1574 E-mail: export@sys.hpk.co.jp Local contact information worldwide can be found at: www.hamamatsu.com...
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