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Hamamatsu Photonics ORCA-Lighting C14120-20P Instruction Manual

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ORCA-Lightning
Digital CMOS Camera
C14120-20P
Instruction manual
Thank you for your purchase
•
Follow the safety precautions in Chapter 1 in order to avoid personal injury
and damage to property when using this camera. The manual describes the
correct handling method of the camera and provides instructions that should
be followed to avoid accidents. Read this manual carefully before using this
camera. After reading this manual, store it in a location where you can refer to
it at any time.
Ver. 2.5
Oct. 2019
HAMAMATSU PHOTONICS K.K.
A6540313-05

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Summary of Contents for Hamamatsu Photonics ORCA-Lighting C14120-20P

  • Page 1 Read this manual carefully before using this camera. After reading this manual, store it in a location where you can refer to it at any time. Ver. 2.5 Oct. 2019 HAMAMATSU PHOTONICS K.K. A6540313-05...
  • Page 2 ~ Blank page ~...

  • Page 3: Safety Precautions

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 1. SAFETY PRECAUTIONS SYMBOLS The symbols shown below are used for this camera. Direct current Alternating current CLASSIFICATION OF WARNINGS We have classified the warnings symbols that appear in this instruction manual and on the camera as follows for your convenience.

  • Page 4: Basic Percautions

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 BASIC PERCAUTIONS WARNING Power supply Use the camera with the indicated voltage on the rating sticker. Using a different voltage can damage the camera and lead to fire or electric shock. Cables Do not place heavy objects on cables or bend them excessively. Doing so can damage the cables and lead to fire or electric shock.
  • Page 5 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 CAUTION AC adapter When unplugging the power supply cord, do not pull on the cord. Remove the plug from the outlet to avoid causing electric shock or fire. When unplugging the power supply cord, do not pull on the cord, but remove the plug from the camera to avoid breakdown of the AC adapter or the camera.

  • Page 6: Check The Contents Of Package

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 2. CHECK THE CONTENTS OF PACKAGE When opening the package, check that the following items are included before use. If the contents are incorrect, insufficient or damaged in any way, contact a Hamamatsu subsidiary or your local distributor before attempting to operate the camera.

  • Page 7: Installation

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 3. INSTALLATION Avoid using or storing this camera in the following places Places where the temperature is not the operating temperature indicated in the specifications • Places where the temperature is not the storage temperature indicated in the specifications •...

  • Page 8: Table Of Contents

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 Contents 1. SAFETY PRECAUTIONS ..................1 SYMBOLS ..........................1 CLASSIFICATION OF WARNINGS ..................1 BASIC PERCAUTIONS ......................2 2. CHECK THE CONTENTS OF PACKAGE ............4 3. INSTALLATION ....................5 4. OVERVIEW ......................8 5.
  • Page 9 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 11-4 OPERATIONS ........................44 11-5 SWITCHING THE COOLING METHOD OF “DCAM CONFIGURATOR” ......45 11-6 DISCONNECTION OF WATER COOLING HOSES ............47 12. MAINTENANCE ....................48 12-1 CARE ............................48 12-2 WHEN USING AT WATER-COOLING .................49 13. TROUBLSHOOTING ..................50 13-1 TURNING ON THE POWER ....................50 13-2 ALTHOUGH IMAGES ARE TRANSFERRED ..............50 13-3 AT WATER-COOLING ......................51...

  • Page 10: Overview

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 4. OVERVIEW C14120-20P is equipped with the new scientific image sensor, an advanced CMOS device that realizes the multiple benefits of high resolution, high readout speed, and low noise all at once. The setting and the control of the camera are operated by the application software. 5.
  • Page 11 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 (5) Readout method of CMOS image sensor The exposure and the readout method of CMOS image sensor which this camera adopts is rolling shutter. In the rolling shutter, the exposure and readout are done four lines and the data of each horizontal line is read by four lines of column amplifier and A/D simultaneously.
  • Page 12 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 Readout methods Readout methods Readout unit Readout area Normal readout 1×1 step (1 pixel) Whole area 2×2 step (2 pixel × 2 pixel Binning readout (Digital binning) Whole area 4×4 step pixel × 4 pixel Sub-array readout 1×1 step (1 pixel) Designated area...

  • Page 13: Name And Function Parts

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 6. NAME AND FUNCTION PARTS [Top] [Front] [Side] [Rear] [Bottom] [Side] Figure 6-1 a. Lens mount f. Power switch b. CoaXPress interface connector 1, 2, 3, 4 g. DC power input connector c. Trigger input connector h.
  • Page 14 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 a. Lens mount This attaches an optical lens or an optical system with an optical lens. It can attach the type of F-mount, C-mount or TFL-II-mount either. It is attached F-mount adapter by default. When used C-mount type, it need to change to the C-mount adapter.
  • Page 15 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 f. Power switch [POWER] The power is turned on/off. When the camera is turned on, the camera starts to initialize and after the initialization is complete, the camera can be operated. g. DC power input connector [DC IN] This is the power supply terminal.

  • Page 16: Cable Connections

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 7. CABLE CONNECTIONS Refer to the figure when connecting the various cables. Camera (Rear panel) a. AC adapter Computer CoaXPress interface cables (Option: A14590-03-40) CoaXPress interface board (Option: M9982-30) Figure 7-1 a. AC adapter This is the cord to supply a power supply.

  • Page 17: Operations

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 8. OPERATIONS This camera can be started after setup. The setting and the control of the camera are operated by the application software. PRECAUTION FOR IMAGING (1) Ambient temperature The recommended ambient temperature for camera operation is 25 °C. The maximum temperatures to which the CMOS image sensor can be cooled, and the stability of the cooled temperature, are affected by the ambient temperature.

  • Page 18: Set Up

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 SET UP First, set up the following before operating the camera. (1) Setup of the computer and installation of an application software Set up a computer to use beforehand, such as installing interface or application software. Perform these procedures according to the each instruction manual.

  • Page 19: Operating Procedure

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 OPERATING PROCEDURE After setup, operate Air-cooling according to the following. Handle the application software and peripherals according to each instruction. SET UP (Section 8-2) ↓ Turn on the camera ↓ Make sure the cooling fan is turning ↓...

  • Page 20: Exchanging / Detaching Of Mount Adapter

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 EXCHANGING / DETACHING OF MOUNT ADAPTER When using the C-mount lens or the TFL-II-mount lens, exchanging or detaching a mount adapter according to the following. When the mount adapter is installed, always secure a space where you can •...
  • Page 21 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 3. Fit the C-mount adapter which is accessory of the camera into the center of the lens mount. Turn it clockwise and tighten the screw surely. C-mount adapter 4. Tighten the 2 screws of the C-mount by using the hexagonal wrench. 5.

  • Page 22: Update Procedure Of Coaxpress Interface Board

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 UPDATE PROCEDURE OF COAXPRESS INTERFACE BOARD If you use an optional CoaXPress interface board, when the driver included in DCAM-API does not match the firmware version of the interface board, the following message prompting update of the interface board may be displayed.

  • Page 23: About Image Acquisition

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 9. ABOUT IMAGE ACQUISITION READOUT METHOD OF THE IMAGE This camera uses scientific CMOS image sensor. The exposure and the readout method of CMOS image sensor which this camera adopts is rolling shutter. In the rolling shutter, the exposure and readout are done four lines simultaneously.

  • Page 24: Camera Settings And Functions

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 10. CAMERA SETTINGS AND FUNCTIONS The camera setting and operating is controlled by the connected computer (the application software). 10-1 SETTINGS AND FUNCTIONS The following are the camera’s settings and functions. Items Outlines [Default] Refer to Camera readout modes Choose the readout method of image.
  • Page 25 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 [Setting parameters] The setting parameters are different by the camera readout modes. (: enabled, −: disabled) Normal Lightsheet Functions Area Mode Readout Mode   Camera operation modes Free running mode   External Edge trigger mode triggermode...

  • Page 26: Camera Readout Modes

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 10-2 CAMERA READOUT MODES This mode is for acquiring the image. There are two following modes. The default is “Normal Area Mode”. The “Lightsheet Readout Mode” is chosen if the lightsheet microscope is used. 10-2-1 Normal Area Mode Images are readout with the timing depending on the horizontal pixel numbers and the vertical line numbers.

  • Page 27: Camera Operation Modes

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 10-3 CAMERA OPERATION MODES This mode is for controlling the timing of readout and exposure. This mode can use; Free running mode, Edge trigger mode (External trigger mode), and Start trigger mode. • Please contact a Hamamatsu subsidiary or your local distributor for the detail of the timing information.
  • Page 28 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 b. Electrical shutter operation or action The electrical shutter operation is used to get a proper signal level when signal overflow happens due to too much input photons in the normal readout operation. In this operation, the fastest frame rate is operated at full resolution even when the exposure time is short.
  • Page 29 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 10-3-2 External trigger mode The camera has various external trigger functions to synchronize the camera with the external equipment. In this mode, the external equipment becomes a master and the camera becomes a slave. The mode has the following kinds.
  • Page 30 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 Figure 10-5 (Example for rising edge in Lightsheet Readout Mode) (2) Global reset Edge trigger mode Global reset function enables to reset the electric charge of all pixels at the same time. Then all pixels can start exposure at the same time.
  • Page 31 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 (3) Level trigger mode The Level trigger mode is used to control both exposure start timing and exposure time length by inputting external trigger pulses. In this mode, the camera starts exposure at the start of high or low period of the input trigger pulse and stops exposure at the end of high or low period of the input trigger pulse.
  • Page 32 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 (4) Global reset Level trigger mode The Global reset function enables to reset the electric charge of all pixels at the same time. Then all pixels can start exposure at the same time. The example below is for the trigger level High.
  • Page 33 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 Figure 10-9 (Example for rising edge) b. Trigger times Also in the Synchronous readout trigger mode, synchronous readout can be controlled by specifying, the number of timing pulses to determine the exposure time. The following figure shows the exposure timing when the Trigger times is set as 3.
  • Page 34 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 10-3-3 Start trigger mode The Start trigger mode is to start operating the camera by a trigger input for a continuous imaging. It is useful to secure the frame rate as fast as possible when continuous image acquisition and not to sacrifice the exposure time.

  • Page 35: Readout Methods

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 10-4 READOUT METHODS The readout methods can set units for reading out images. In the Sub-array readout, the readout area is also set. The methods have the below. (: enable, −: disable) Normal Lightsheet Unit of readout pixel Readout area...

  • Page 36: Readout Time Of Horizontal Line

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 10-5 READOUT TIME OF HORIZONTAL LINE Readout time of the horizontal line is varied depending on the horizontal pixel number set in the Sub-array readout mode. Vn = number of vertical lines Readout time = (Vn+2) × 4H 4H = readout time of one line The readout time per one line (4H) can be calculated by the following formula: (1) In Normal Area Mode...

  • Page 37: Trigger Outputs

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 10-7 TRIGGER OUTPUTS The camera provides a range of trigger output signals to synchronize with an external instrument and the camera becomes the master and the external instrument becomes the slave. There are three different trigger output functions as follows. - Global exposure timing output - Programmable timing output - Trigger ready output...
  • Page 38 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 (2) Programmable timing output a. In Normal Area Mode By using the programmable timing output, synchronizing external equipment is simple. A system that needs simple timing signal does not require a delay unit or pulse generator. It is possible to program and output a pulse that has an optional pulse width and an optional delay time to Read End (the end of readout timing) or Vsync.
  • Page 39 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 b. In Lightsheet Readout Mode By using the programmable timing output, synchronizing with external devices is simple. A system which needs simple timing signal does not require a delay unit or pulse generator. It is possible to program and output a pulse that has an optional pulse width and an optional delay time to Read End (the end of readout timing), Vsync or Hsync.

  • Page 40: Real-Time Defect Pixel Correction

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 Figure 10-15 (Example for Programmable timing output with Hsync in Lightsheet Readout Mode, forward direction readout) When you choose Hsync for the reference of programmable timing output, camera can output some pulses before start the exposure. It is called as Pre-Hsync. You can set the number of Pre-Hsync.

  • Page 41: High Full Well Capacity Mode

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 10-9 HIGH FULL WELL CAPACITY MODE The High Full Well Capacity Mode can increase a full well capacity from 1000 electrons to 38 000 electrons and enable 16 bit digital output. • Switching Standard Full Well Capacity mode / High Full Well Capacity mode is done by HighDynamicRange Mode Off/On in DCAM property.
  • Page 42 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 [Calculation example] Frame rate (fps) Synchronous Free running Edge trigger Global readout mode mode reset trigger mode 4608 2592 2688 2592 2560 2560 2048 2048 Standard Full Well Capacity mode 1024 1024 1201 1175 1210 1166...

  • Page 43: When Using Water-Cooling Method

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 11. WHEN USING WATER-COOLING METHOD Water-cooling method is used when the camera is used in a place where the air conditioning is unstable. When using Water-cooling method, follow this chapter and chapter 8. •...

  • Page 44: Preparing

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 If the hose size on circulating water cooler is the same as blue hose, remove gray hose from the joint part. The gray hose can be removed when blue hose is pulled with pushing the button of the joint on gray hose.

  • Page 45: Connections

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 11-3 CONNECTIONS Connect the cooling water hoses to the connectors of the circulating water cooler. Camera (Rear panel) Cooling water hoses (2 hoses) Circulating water cooler Computer CoaXPress interface cables (4 cables) Figure 11-1 •...

  • Page 46: Operations

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 11-4 OPERATIONS After setup, operate Water-cooling according to the following. Handle the application software and peripherals according to each instruction. SET UP (Section 8-2, 11-2, 11-3) ↓ Switch on the cooling method by DCAM Configurator Turn on the camera and the computer ↓...

  • Page 47: Switching The Cooling Method Of "Dcam Configurator

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 11-5 SWITCHING THE COOLING METHOD OF “DCAM CONFIGURATOR” (1) Notices - When the camera is shipped, the cooling method for the camera is set for “Air-cooling”. (It is unnecessary to exchange it when the camera is just used at “Air-cooling”.) - The setting is not changed even if the power supply of the camera is turned off.
  • Page 48 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 3. The items of window are changed as follows. Click the “DCAM Configurator”. 4. “DCAM Configurator” is started. “DCAM-API Configurator” window is displayed. Click the “Hardware” icon, and set the cooling method to Water-cooling. Refer to the instruction manual of DCAM Configurator about how to operate the “DCAM Configurator”...

  • Page 49: Disconnection Of Water Cooling Hoses

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 11-6 DISCONNECTION OF WATER COOLING HOSES Remove the water cooling hoses only when it is necessary to remove. Cooling water may be left inside the camera even after hoses are removed. In • such case, remove water inside by blowing air from connectors.

  • Page 50: Maintenance

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 12. MAINTENANCE 12-1 CARE (1) Camera body Perform cleaning of this equipment with the dry soft cloth. • Do not wipe with a damp cloth or unclean cloth. (2) Glass window on the image sensor 1.

  • Page 51: When Using At Water-Cooling

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 12-2 WHEN USING AT WATER-COOLING (1) Circulating water cooler • Regarding handling cooling water and circulating water cooler, refer to instruction manual attached to the circulating water cooler. (2) Cooling water • It is recommended to use soft water (except pure water) for cooling water. •...

  • Page 52: Troublshooting

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 13. TROUBLSHOOTING If an abnormality occurs, look up the possible causes in the following tables and, if necessary, report the details to Hamamatsu subsidiary or your local distributor. When the following measures are taken, turn off the camera and peripherals •...

  • Page 53: At Water-Cooling

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 13-3 AT WATER-COOLING Symptom Cause Measures Chapter The power supply of circulating Turn on the power supply of the water cooler is not turning on circulating water cooler. The cooling method of DCAM Change the setting to 11-5 Cooling or circulation...

  • Page 54: Specifications

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 14. SPECIFICATIONS (1) Performances Imaging device Scientific CMOS image sensor Effective number of pixels 4608 (H) × 2592 (V) 5.5 μm (H) × 5.5 µm (V) Cell size Effective area 25.344 mm (H) × 14.256 mm (V) Cooling method Peltier device + Forced air-cooling, Water-cooling at Forced air-cooling...
  • Page 55 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 (2) Functions Camera readout modes [Correspondence table by camera readout modes] (: enabled, −: disabled) Normal Lightsheet Functions Area Mode Readout Mode   Camera operation modes Free running mode   External trigger mode ...
  • Page 56 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 d. Trigger outputs Global exposure timing output Programmable timing output 1 Programmable timing output 2 External signal output Programmable timing output 3 Trigger ready output Continuous High output Continuous Low output External trigger output level 3.3 V LVCMOS level 33 Ω...
  • Page 57 ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 (7) Spectral response characteristics (Typical) Graph 14-1...

  • Page 58: Dimensional Outlines

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 15. DIMENSIONAL OUTLINES 15-1 F-MOUNT TYPE (Unit: mm)

  • Page 59: C-Mount Type

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 15-2 C-MOUNT TYPE (Unit: mm)

  • Page 60: Tfl-Ii-Mount Type

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 15-3 TFL-II-MOUNT TYPE (Unit: mm)

  • Page 61: Warranty

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 16. WARRANTY Hamamatsu Photonics have fully inspected this camera and checked that its performance conforms to specifications. In the unlikely event of a breakdown or other malfunction, contact a Hamamatsu subsidiary or your local distributor.

  • Page 62: Contact Information

    ORCA-Lightning Digital CMOS Camera C14120-20P Instruction manual_Ver.2.5 17. CONTACT INFORMATION Manufacturer HAMAMATSU PHOTONICS K. K., Systems Division 812 Joko-cho, Higashi-ku, Hamamatsu City, Shizuoka Pref., 431-3196, Japan Telephone (81) 53-431-0124, Fax: (81) 53-435-1574 E-mail: export@sys.hpk.co.jp Local contact information worldwide can be found at: www.hamamatsu.com...

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