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Cole Parmer CG-500 Operation Manual

Cryogenic grinder designed for milling tough and temperature sensitive samples
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CG-500 Freezer/Mill®
Cryogenic Grinder Designed for Milling Tough and Temperature Sensitive Samples
Operation Manual
For 115V (04500-42) and 230V (04577-94)
1300-87052-1M, REV 6

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  • Page 1 CG-500 Freezer/Mill® Cryogenic Grinder Designed for Milling Tough and Temperature Sensitive Samples Operation Manual For 115V (04500-42) and 230V (04577-94) 1300-87052-1M, REV 6...
  • Page 2 08840. SPEX SamplePrep is now part of Cole-Parmer®. The Cole-Parmer® CG-500 Freezer/Mill was formerly known as SPEX 6875D Freezer/Mill. Over the years, we’ve acquired many high-quality and reputable brands. After many years of continual growth, we realize our brands are all as brilliant as each other. Rather than have a portfolio of complementary brands, we felt consolidating them under one world- class brand name enabled us to offer a single and significant brand experience.

  • Page 3: Table Of Contents

    CG-500 Freezer/Mill TABLE OF CONTENTS SECTION DESCRIPTION PAGE Introduction ........................4 Specifications ........................5 Unpacking ........................... 6 Setting Up ..........................7 Electrical Connection ......................7 Power Switch and Controller .................... 8 Liquid Nitrogen Connection………………………………………………………………………………………….9 Lid, Coil Assembly and Gate, LN Sensor ................10 Grinding Vial Sizes, Adapters, and Vial Openers ............

  • Page 4: Introduction

    They are the “mills of first choice” for many abnormal materials, or samples whose composition or structure cannot be ground using conventional grinding methods. NOTE: Do not operate the CG-500 Freezer/Mill until you have read the Operating Manual and are familiar with the controls and operation.

  • Page 5: Specifications

    CG-500 Freezer/Mill 2.0 CG-500 FREEZER/MILL SPECIFICATIONS Type of Mill: Dual Chamber Cryogenic impact mill Controller: Touchscreen Data Transfer: Dual USB Port (back of controller) Grinding Mechanism: Steel impactor driven by magnetic coil Coolant: Liquid nitrogen (LN) Dimensions: 20 in. (50.8 cm) x 21½ in. (55 cm) x 25½ in. (64.77 cm) Weight: (empty, without vial or coolant) approx.

  • Page 6: Unpacking

    6757 for microvials. The 6870S also includes a vial holder for running up to four small vials per CG-500 grinding chamber. The full range of grinding vials and accessories for the CG-500 Freezer/Mill is described in our catalog, The Freezer/Mill Accessory Manual, and at www.Cole-Parmer.com.

  • Page 7: Setting Up

    Figure 2 – CG-500 Freezer/Mill, Rear View 4.0 SETTING UP The CG-500 Freezer/Mill weighs 78 pounds (35.38 Kg) empty. The lid opens from the front with the lid latch in the center. The AC Input module and the ON/OFF switch (which also serves as a circuit breaker) are on the back of the cabinet.

  • Page 8: Power Switch And Controller

    The viewing angle of the controller on the CG-500 Freezer/Mill can be adjusted to minimize glare. To adjust the viewing angle, simply grasp the back of the controller box and gently pull it forward until the desired angle is reached, as shown in Figure 3.

  • Page 9: Liquid Nitrogen Connection

    (Figure 4). Then attach the LN transfer hose to the safety valve, and the other end to the inlet on the CG-500 Freezer/Mill. The safety valve should be higher than the LN inlet on the Freezer/Mill. The purpose of the safety valve is to provide an exit for liquid nitrogen vapor in case the mill and LN Tank valves are both closed, and pressure builds up inside the hose.

  • Page 10: Lid, Coil Assembly And Gate, Ln Sensor

    Chamber 1 or Chamber 2. Do not run the CG-500 Freezer/Mill without a loaded sample vial in the grinding Chamber(s) that is active (programmed to grind).

  • Page 11: Grinding Vial Sizes, Adapters, And Vial Openers

    The liquid nitrogen (LN) sensor is located behind a shield on the back wall of the LN tub. While in Auto Fill mode, with the CG-500 Freezer/Mill connected to the LN Tank, the unit will add liquid nitrogen automatically to maintain the proper LN level. If the unit is started and the LN Level is low, the screen will display status as LOW LN LEVEL.

  • Page 12: Touchscreen Display

    6757V Microvials. Microvials can be opened using the 6758 Extractor. 5.0 TOUCHSCREEN DISPLAY The CG-500 Freezer/Mill is programmed and operated through a series of touchscreen displays. Transitioning between screens, and all programming and operating commands, are done by touching the screen with a fingertip or stylus.

  • Page 13: Control Panel

    CG-500 Freezer/Mill 5.2 Control Panel The Control Panel displays the programmed run parameters, as shown in Figure 6. Changes to the run settings, and to activate Mono or Dual grinding modes are made from this screen. In Mono mode the programmed run parameters will Only operate Chamber 1. If Dual mode is selected the run parameters (Figure 6), will operate Chamber 1 and Chamber 2 simultaneously.
  • Page 14 In Figure 6, Auto-Fill or Manual Fill mode has not been selected (note the buttons are gray). The CG-500 Freezer/Mill must be connected to a LN Tank or LN Line to run in Auto Fill mode. To change from Auto Fill mode to Manual Fill mode, touch the Manual button, the button will change from gray to blue indicating the unit will now be filled manually.
  • Page 15 CG-500 Freezer/Mill 5.2 CONTROL PANEL (Cont’d) If the LN Tank is empty and unable to fill the tub with liquid nitrogen change the LN Tank and touch RESUME to begin operation. If the lid is not completely closed and latched, the screen will display LID OPEN, as shown in Figure 8.
  • Page 16 CG-500 Freezer/Mill 5.2 CONTROL PANEL (Cont’d) Following the PRECOOL stage is the first GRINDING cycle stage. The RUN TIME line will display the time remaining in that cycle of the grinding stage. At the end of each grinding period a green check mark is placed next to that field.

  • Page 17: Changing Settings

    CG-500 Freezer/Mill 5.2.2 Adjusting the Grinding Rate While in the CONTROL PANEL screen, the only parameter that can be modified during grinding is the RATE. It is adjusted by touching the minus (-) and plus (+) buttons next to the Rate field.

  • Page 18: Saved Protocols

    CG-500 Freezer/Mill 5.4 Saved Protocols The SAVED PROTOCOLS screen is shown in Figure 12. In this example, three saved protocols have been stored and named (e.g. Hair, Bone, Soft Tissue). Up to 20 Protocols can be stored. access or make visible additional protocols, or vacant protocol spaces (e.g. User Protocol) swipe up or down the touchscreen.
  • Page 19 CG-500 Freezer/Mill 5.4 Saved Protocols (Cont’d) The Up/Down yellow arrow key on KEYBOARD allows the user to shift back and forth from upper case to lower case (the default is upper case). Touch Down arrow to switch to lower case. Or touch Up arrow to revert to upper case.

  • Page 20: Operation

    6.1 Standard Auto Fill When the CG-500 Freezer/Mill is set up for the first run of the day, with a loaded vial(s) in the grinding Chamber(s), make sure the unit is connected to a LN Tank or LN line and the LN valve is open.

  • Page 21: Manually Adding Liquid Nitrogen

    Liquid nitrogen (LN) should only be added to CG-500 Freezer/Mill with the lid open. To open the lid, hold the lid down with one hand, and with the other hand pull the upper end of the latch toward you.
  • Page 22 CG-500 Freezer/Mill 6.2 Manually Adding Liquid Nitrogen (Cont’d) Once more, slowly lower the lid and secure the latch. Caution: When the lid is closed and latched, nitrogen vapor which vents from the Freezer/Mill can displace the oxygen in a closed room and cause asphyxiation.
  • Page 23 CG-500 Freezer/Mill 6.2 Manually Adding Liquid Nitrogen (Cont’d) • Grind longer. (In Manual Fill mode, 20 minutes of actual grinding time is the maximum recommended for one run, due to the liquid nitrogen capacity of the tub, but you can top off the LN and repeat the run if needed.)

  • Page 24: Loading Vials Into The Freezer/Mill

    CG-500 Freezer/Mill 6.2.5 Loading a Very Small Sample into a Vial and Keeping it Cold A similar loading technique to Section 6.2.4 can be used to grind very small samples with an upright, open pre-chilled vial. Place the sample on the chilled end plug and add a small amount of fluid such as an extraction medium or water.
  • Page 25 CG-500 Freezer/Mill 6.5 Opening, Emptying, and Cleaning Freezer/Mill Vials (Cont’d) polycarbonate center section. The best way to prevent the Extractor from jamming is to make sure both the Extractor and the threaded end plugs are dry and clean before you use them.
  • Page 26 CG-500 Freezer/Mill 6.5 Opening, Emptying, and Cleaning Freezer/Mill Vials (Cont’d) If it is necessary to recover 100% of the sample, let the vial warm up and add some water or other liquid that will not affect the polycarbonate (Section 6.5.3). Shake the vial, empty it onto filter paper, and rinse again if necessary to recover the entire sample.

  • Page 27: Checking The Liquid Nitrogen Level

    CG-500 Freezer/Mill 6.6 Checking the Liquid Nitrogen Level In Manual Fill mode only, every time a vial is inserted into or removed from the Freezer/Mill, note the level of liquid nitrogen in the tub. If the LN level is Low the mill will not run. Add LN as needed.

  • Page 28: System Settings

    Figure 16 – Settings Screen 9.0 MAINTENANCE The CG-500 Freezer/Mill has been designed to provide trouble-free operation over a long period of time. To assure proper performance it is very important to keep the unit clean. When the Freezer/Mill is at room temperature, any spilled powders or liquids should be wiped up immediately.

  • Page 29: Trouble-Shooting Guide

    CG-500 Freezer/Mill 10.0 TROUBLE-SHOOTING GUIDE PROBLEM POSSIBLE CAUSE POSSIBLE SOLUTION No Power Make sure power cord is plugged into outlet and inlet. No Power Check outlet for power and The unit does not turn on. correct as required. Circuit Breaker tripped Turn unit OFF then ON.
  • Page 30 CG-500 Freezer/Mill 10.0 TROUBLE-SHOOTING GUIDE (Cont’d) PROBLEM POSSIBLE CAUSE POSSIBLE SOLUTION Water in vial has frozen the Remove vial and replace vial impactor. and contents with a dry unit or tap ends of the vial on counter to free impactor.

  • Page 31: Warranty

    CG-500 Freezer/Mill 11.0 WARRANTY Cole-Parmer® guarantees its products against defects in materials or workmanship for three years from the date of original shipment. Repairs, replacements, or parts are guaranteed for 30 days or for the remaining original warranty period (whichever is greater) for the item that was repaired or replaced.

  • Page 32: Instrument Disposal

    CG-500 Freezer/Mill 12.0 INSTRUMENT DISPOSAL In accordance to the EU Directive 2012/19/EU covering Waste Electrical and Electronic Equipment, all equipment with the disposal symbol must not be disposed of with general waste. (See Figure 17) Disposal Label is located on the back of unit.

  • Page 33: Contact Us

    CG-500 Freezer/Mill 13.0 CONTACT US Repair Service Phone: 1.732.623.0465 Cole-Parmer 65 Liberty St Metuchen, NJ 08840 Attn: Service and Repair Please include RA Number on the shipping label. Germany T: +1.800.323.4340 or T: +49 (0) 9377 92030 +1.800.323.4340 E: de.sales@antylia.com E: sales@antylia.com...

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