Sartorius mLINE 725010 User Manual

Sartorius mLINE 725010 User Manual

Mechanical pipette
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mLINE®
Mechanical
Pipette
User Manual

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Summary of Contents for Sartorius mLINE 725010

  • Page 1 mLINE® Mechanical Pipette User Manual...
  • Page 2: Table Of Contents

    Contents 1. Intended use ..................... 2 2. Product description ..................2 3. Sartorius pipette tips ..................4 4. Unpacking ......................4 5. Personal identification .................. 4 6. Pipette holder and carousel stand ............5 6.1. Installing the pipette holder ............5 6.2.
  • Page 3: Intended Use

    The Sartorius mLINE® pipette is intended, designed and manufactured for dispensing liquids in a variety of applications, and to be used in combination with Sartorius Optifit Tips or SafetySpace Filter Tips. The Sartorius pipette and tip combination, fall within the scope of in-vitro diagnostics, and can be used as a diagnostic medical device in related applications.
  • Page 4 Pipette description Cap ....... ID window and tag Operating button ....ejector ..Finger support ..Handle Display ..... Volume ... Calibration lock ....Colour coding....Tip ejector collar ...... Tip cone....Safe-Cone ..Filter ..Tip cone housing Tip cone .......
  • Page 5: Sartorius Pipette Tips

    Sartorius tips are made of pure virgin polypropylene and manufactured in protected clean room conditions. Sartorius offers a full range of standard Optifit, and SafetySpace™...
  • Page 6: Pipette Holder And Carousel Stand

    2. Remove the tag and mark the identifying information on it. 3. Position the tag in place and clip the window back into place. 6. Pipette holder and carousel stand For convenience and safety, always keep the pipette vertically on its own holder or carousel stand when not in use.
  • Page 7: Sealing And Ejecting Tips

    8.2. Sealing and ejecting tips To ensure the maximum accuracy and precision, Sartorius tips are recommended to be used with mLINE pipettes. Before fitting a tip ensure that Tip ejector the pipette tip cone is clean. Press the tip on the tip cone of the pipette.
  • Page 8: Pipetting Techniques

    Pipetting Techniques Two basic pipetting techniques, forward and reverse pipetting, are associated with mLINE pipettes. Forward pipetting is the most common used pipetting technique. The technique employs the blow-out function ensuring complete delivery of the liquid. Reverse pipetting is recommended for highly viscous, biological or foaming liquids, or very small volumes of liquid.
  • Page 9: Repetitive Reverse Technique

    (operating temperature from 15°C to 40°C). 11. Maintenance mLINE pipettes have been designed for easy in-house service. If the pipette is used daily it is recommended to clean/decontaminate and check the performance of the pipette every three months. Sartorius also provides complete...
  • Page 10: Daily Cleaning Of The Outer Surface Of The Pipette

    If your pipette is in daily use it is recommended to clean/decontaminate and grease the pipette every three months. It is recommended to send the multichannel pipettes to your local Sartorius service representative for cleaning and greasing. Hold tip To clean and decontaminate the lower parts of...
  • Page 11 Hold the tip cone holder firmly with your fingers and turn the tip cone cylinder counter clockwise with the other hand. Pipette 10 ml: Hold the tip cone cylinder firmly and turn the tip cone holder clockwise with your fingers. Note: Don't turn the tip cone cylinder + holder system together.
  • Page 12: Sterilization Of The Pipette

    Note: It is always necessary to check the performance of the pipette after in-house service or maintenance. 11.3. Sterilization of The Pipette The entire mLINE pipette can be sterilized by steam, autoclaving at 121°C, (252°F), 1 atm for Connecting 20 minutes. Remove the tip cone filter (if fitted, collar Chapter 8.3).
  • Page 13: Testing The Performance And Recalibration

    12. Testing the Performance and Recalibration It is recommended to check the performance of your mLINE pipettes regularly (e.g. every 3 months) and always after in-house maintenance. However, the user should establish a regular testing routine for their pipettes with regard to accuracy requirements of the application, frequency of use, number of operators using the pipette, nature of the liquid dispensed and...
  • Page 14 (ISO 8655-1.) Note: Sartorius specifications are achieved in strictly controlled conditions (ISO 8655-6). The user should establish own specifications based on the field of use and the accuracy requirements placed on the pipette (ISO8655-1).
  • Page 15: Recalibration

    – test method for volumetric instruments. The pipette's specifications are guaranteed only with genuine Sartorius tips. If you find the pipette to be inaccurate after performance testing, please follow the instructions below: 1. Remove the lid of the calibration nut, located on the back of the handle, with the aid of the calibration tool.
  • Page 16: Troubleshooting

    13. Troubleshooting Symptom Possible cause Solution Droplets left Unsuitable tip Use original inside the tip Sartorius tips Leakage or pipetted Non uniform wetting Attach new tip volume too small of the plastic Tip incorrectly Attach firmly attached Unsuitable tip Use original...
  • Page 17: Warranty Information

    INSTRUCTIONS GIVEN IN THIS MANUAL OR WITH OTHER THAN THE MANUFACTURER’S ORIGINAL TIPS. Each mLINE pipette is tested before shipping by the manufacturer. The Sartorius Quality Assurance Procedure guarantees that the mLINE pipette you have purchased is ready for use.
  • Page 18: Performance Specifications

    N Note: The listed systematic and random error values can be achieved only under strictly controlled conditions during type tests per ISO 8655. Due to the continuous product development by Sartorius. the systematic and random error values are subject to change without prior notice...
  • Page 19 The status of the information, specifications and illustrations in this manual is indicated by the date given aside. Sartorius reserves the right to make changes to the technology, features, specifications and design of the equipment without notice. All trademarks are Sartorius property unless...

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