Table of Contents
Advertisement
Quick Links
Advertisement
Table of Contents
Related Manuals for LI-COR Odyssey CLx 9140
Summary of Contents for LI-COR Odyssey CLx 9140
- Page 1 ® Operator’s Manual...
- Page 3 Notes on Safety ® LI-COR products have been designed to be safe when operated in the manner described in this manual. The safety of this product can not be guaranteed if the product is used in any other way than is specified ®...
- Page 4 LI-COR is an ISO9001 registered company. © 2011 LI-COR Inc. All rights reserved. Specifications subject to change. LI-COR, Odyssey, MPX, IRDye, MousePOD, and In-Cell Western are trademarks or registered trademarks of LI-COR Inc. The Odyssey CLx Imager is covered by U.S.
-
Page 5: Table Of Contents
ODYSSEY Table of Contents Chapter 1: Installation and Safety Information Safety Considerations..............1 Laser Safety . - Page 6 Chapter 3: Operation Before You Begin..............13 Cleaning the Scanning Surface .
-
Page 7: Safety Considerations
ODYSSEY Chapter 1 Installation and Safety Information Safety Considerations Laser Safety The Center for Devices and Radiological Health (CDRH) was established in October 1982, by the U.S. Food and Drug Administration (FDA) to protect the public health in the fields of medical devices and radiological health. -
Page 8: Safety Interlocks
Chapter 1: Installation and Safety Information Figure 1-2. Manufacturer label. VISIBLE AND INVISIBLE LASER DANGER: RADIATION WHEN OPEN AVOID DIRECT EXPOSURE TO BEAM RAYONNEMENT VISIBLE ET INVISIBLE LASER EN CAS DANGER: D'OUVERTURE EXPOSITION DANGEREUSE AU FAISCEAU Figure 1-1. CDRH regulation compliance label. -
Page 9: Placement In The Laboratory
Power in Chapter 2. Be cautious and use a minimum of two people, one on each side of the instru- ® ment, when moving the LI-COR Odyssey CLx Imager, as it weighs about 33 kg (72 lbs). Lift under the metal enclosure on each side of the unit, and keep the unit as near vertical as possible. -
Page 10: Chemical Safety
Figure 1-5. Turn the metal inserts on the feet to adjust the height. Chemical Safety LI-COR Biosciences recommends that all biochemicals be handled carefully, and that safe laboratory procedures be followed at all times. Be aware of the hazards associated with any chemical before you begin work. -
Page 11: Power Switch
LI-COR Biosciences recommends that you routinely inspect the system and the scanning surface. The following are some general maintenance guidelines: •... -
Page 12: Networking Cables
Chapter 1: Installation and Safety Information Networking Cables A Cat. 5e network cable is provided with the Odyssey CLx Imager. Do not use networking cables other than the one provided. The use of other networking cables may result in improper electrical performance. The networking port is on the back panel of the instrument and is marked with the label illustrated in Figure 1-7. -
Page 13: Chapter 2: System Overview
ODYSSEY Chapter 2 System Overview External Panels and Controls Front Panel and Scanning Surface Scanning surface Green image acquisition indicator (shown unlit) Red error condition Power on/off indicator button (shown unlit) ® Figure 2-1. Front panel features and power button on the Odyssey CLx Imager. -
Page 14: Rear Panel
Chapter 2: System Overview Green Image Acquisition Indicator: This indicator light blinks during image acquisition and is contin- uously on at other times, as long as communication with Image Studio software is maintained. The indicator light is off before the instrument is connected to the software and if the Image Studio software is closed or communication is otherwise lost. -
Page 15: Scanning Surface
® Odyssey CLx Imager Scanning Surface The scanning surface is a 25 cm x 25 cm glass plate (Figure 2-2), upon which the samples to be scanned are placed. The scanning surface is sealed from the instrument interior so no moisture can penetrate to the detection optics and electronics below. -
Page 16: Optical System Description
Chapter 2: System Overview Optical System Description Solid state diode lasers simultaneously provide light excitation at 685 and 785 nm. Collimating lenses, optical bandpass filters, and a focusing lens focus and tune the laser beams to produce an excitation spot on the scanning surface. -
Page 17: Image Studio Software Overview
® Odyssey CLx Imager Image Studio Software Overview Learning the Software Each part of the user interface (button, etc.) has an extensive tool tip associated with it that can be invoked by hovering the mouse over the feature on the user interface. A more comprehensive review of acquiring images as well as analysis functions can be found in the Image Studio Software Help system. -
Page 19: Chapter 3: Operation
ODYSSEY Chapter 3 Operation Before You Begin... The following procedure is recommended before every scan: 1) Thoroughly clean the glass scanning surface with ultrapure water and wipe with a lint-free tissue. 2) Repeat the wash with isopropanol to remove any visible smears. Use ethanol to remove any remain- ing residues. -
Page 20: Using Gels
Chapter 3: Operation •Use the silicone mat included to cover the membrane(s) before scanning. Use the 4" soft roller included to remove any air bubbles that may be present. These optional steps help keep the membrane flat against the scanning surface for optimum imaging. •Protect the membrane from light until it has been scanned. - Page 21 Consult protocols in the Application Protocols manual or on the LI-COR Bio Technical Resources Library (http://biosupport.licor.com) for specific recommendations. •Do not use plates with white walls because the autofluorescence from the white surface will create significant noise.
-
Page 22: Using The Mousepod Accessory
Chapter 3: Operation Using the MousePOD Accessory ® ® The optional Odyssey MousePOD Accessory fits over the Odyssey CLx scanning surface for in vivo imaging of up to three mice or rats in a temperature-controlled enclosure. Operation of the MousePOD Accessory is described in the Odyssey In vivo Imaging Guide avail- able at: http://biosupport.licor.com Positioning Membranes and Gels... -
Page 23: Positioning Microplates
Image Studio software by hovering over the Status group on the Acquire ribbon. LI-COR Technical Support may ask you to provide copies of the instrument log files to aid in diagnosing problems. The log files can be archived in a standard zip file by choosing File > Zip Select Event Logs. -
Page 25: Chapter 4: Appendix
ODYSSEY Chapter 4 Appendix Specifications Imaging System Laser Lifetime: 40,000 hours typical. 700 Channel Laser Source: Solid-state diode laser at 685 nm. 800 Channel Laser Source: Solid-state diode laser at 785 nm. Detectors: Silicon avalanche photodiodes. Scanning Speed: 5-40 cm/s. Resolution: 21-337 µm.
Need help?
Do you have a question about the Odyssey CLx 9140 and is the answer not in the manual?
Questions and answers