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LI-COR Odyssey XF Operator's Manual

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Odyssey® XF
Operator's Manual

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Summary of Contents for LI-COR Odyssey XF

  • Page 1 Odyssey® XF Operator’s Manual...
  • Page 3 CE Marking: This product (model number 2802) is a CE-marked product. For conformity information, contact LI-COR Support at http://www.licor.com/biotechsupport. Outside of the U.S., contact your local sales office or distributor. 奥德赛的Fc/XF成像仪 Odyssey Fc Imager and Odyssey XF Imager 部件名称 有毒有害物质或元素...
  • Page 4 Notes on Safety LI-COR products have been designed to be safe when operated in the manner described in this manual. The safety of this product cannot be guaranteed if the product is used in any other way than is specified in this manual.
  • Page 5 LI-COR’s or its licensors’ names or any of their respective trademarks. LI-COR is ISO 9001:2015 certified. © 2022 LI-COR, Inc. All rights reserved. Specifications subject to change. The Odyssey XF Imaging System, FieldBrite XT technology, and IRDye reagents are covered by U.S. patents, foreign equivalents, and patents pending.

  • Page 6: Table Of Contents

    Right-side and Rear Panels Optical System Description Imaging Drawer Imaging Drawer Safety Sample Placement Sticking Imaging Drawer Image Acquisition Using Membranes Using Gels Software Overview LI-COR® Acquisition Software for Image Acquisition Empiria Studio® Software for Analysis Detection Channel Summary Page 6...
  • Page 7 Chapter 3: Maintenance Routine Maintenance Clean Imager Clean Tray Cleaning the Fan Filter Front Panel Warning Light Indicates an Error Desiccant Replacement Desiccant Replacement Instructions Obtaining Technical Support Chapter 4: Appendix Specifications Embedded Firmware Source Code Page 7...

  • Page 9: Chapter 1: Installation And Safety Information

    Safety Act of 1968 are required to furnish various reports to the CDRH. The Odyssey XF Imager is certified as a Class I laser product. This means that hazardous laser radiation is not emitted outside the instrument. Radiation emitted inside the Odyssey XF Imager is confined within protective housings and external covers.

  • Page 10: Safety Interlocks

    Where there is a potential for class 4 laser radiation behind a given panel, the label in Figure 3-4 is affixed to the panel. The label shown in Figure 3-4 is affixed to the Odyssey XF in two locations; one on top of the camera hood and one on the inner front access panel (Figure 3-3).

  • Page 11: Placement In The Laboratory

    Space Requirements The Odyssey XF Imaging System requires an area 41.4 cm W (16.3") × 47 cm D (18.5") × 67.3 cm H (26.5"). Depth with the imaging drawer open is 59.7 cm (23.5”). Level the Imager Level the imager to prevent the imaging drawer from scraping on anything and to keep the imager from tilting.

  • Page 12: Chemical Safety

    This is a necessary safety feature. If you are unable to insert the plug into the outlet, you will need to replace the outlet. Do not defeat the purpose of the grounding-type plug. Do not locate the Odyssey XF Imager where the power cord will be walked on or exposed to water or chemical spills.

  • Page 13: Chapter 2: System Overview

    External Panels and Controls Front Panel Figure 4-1. Front panel features and controls of the Odyssey XF Imager. Power On/Off Button: Pressing this button momentarily turns the instrument on and off. While the instrument is performing its startup or shutdown procedure, the blue indicator light in the power button blinks.
  • Page 14 Green Image Acquisition Indicator: This indicator light blinks during image acquisition and is continuously on at other times, as long as communication with LI-COR® Acquisition Software is maintained. The indicator light is off only if communication is lost or LI-COR® Acquisition Software is closed or the instrument is powered off.

  • Page 15: Right-Side And Rear Panels

    Before image acquisition starts, the imaging tray containing a membrane or gel is placed in the Odyssey XF imaging drawer and the drawer is closed. A safety interlock prevents operation of the laser illumination module while the imaging drawer is open.
  • Page 16 (such as SYBR Safe) can be imaged in the 600 nm channel. The quality of images from the Odyssey XF Imager is enhanced by a patented filtering system that dramatically reduces noise before detection by the CCD detector. Infrared signal detection has also been optimized for LI-COR®...

  • Page 17: Imaging Drawer

    During an unexpected power loss, the drawer button will not function. This may leave a sample inside the Odyssey XF Imager, or it may result in the imaging drawer being left partially open or closed. If the power remains off, the drawer can be opened or closed by pulling or pushing on the drawer front.

  • Page 18: Sample Placement

    Chapter 2: System Overview Sample Placement The Odyssey® XF Imaging Tray has four corner markers that delineate the edges of the Odyssey XF imaging area. Place membranes or gels up to 10 x 12 cm on the imaging tray within the corner markers for the entire membrane or gel to be imaged.

  • Page 19: Sticking Imaging Drawer

    Do not use acetone or any other harsh solvents to clean the imaging tray. Sticking Imaging Drawer If the Odyssey XF imaging drawer does not open and close smoothly, contact LI-COR technical support for instructions about acquiring the correct lubricant and correctly applying the lubricant.
  • Page 20 NIR Western blot membranes can be imaged wet or dry. Place the membrane sample-side up in the imaging tray, and place the imaging tray into the imaging drawer. LI-COR Acquisition Software Steps for Scanning Membranes 1. Start LI-COR Acquisition Software 2.

  • Page 21: Using Gels

    Resolution: 125 µm Fluorescence Acquisition Time Options: 30 sec, 1 min, 2 min (default), 5 min, 10 min Software Overview Below is an overview of the software available to you for your Odyssey XF Imager. LI-COR® Acquisition Software for Image Acquisition LI-COR Acquisition Software is the latest software to acquire images using your Odyssey XF Imager.

  • Page 22: Empiria Studio® Software For Analysis

    Use Empiria Studio to analyze NIR Western blot images, chemiluminescent Western blot images, or gel images acquired on the Odyssey XF. To get started using Empiria Studio, please see see licor.com/empiria-support. Detection Channel Summary...

  • Page 23: Chapter 3: Maintenance

    CAUTION: Remove the tray and its contents from the instrument prior to any maintenance that requires moving or tilting the instrument. The Odyssey XF Imager requires only minimal maintenance. However, as with any equipment utilizing electrical voltages, there is a danger of fire or electrical shock if the equipment is not properly maintained.

  • Page 24: Cleaning The Fan Filter

    When the Odyssey® XF Imager is unable to complete a task requested by LI-COR Acquisition Software, or some hardware failure is encountered, the red error indicator on the Odyssey XF front panel (Figure 4-1 and Figure 4-2) flashes and an audible alarm sounds. This error condition is automatically cleared after a few seconds and a message dialog is displayed in the LI-COR Acquisition Software to explain the error.

  • Page 25: Desiccant Replacement Instructions

    When the internal relative humidity reaches 30%, the software will provide a warning message. When the relative humidity reaches 60%, the cooler will be disabled to prevent condensation. The desiccant is user-replaceable. If needed, please contact LI-COR Biosciences for a desiccant replacement kit (9957-046) to perform this task.
  • Page 26 Chapter 3: Maintenance 7. Lift the black plastic cover. 8. The brass desiccant cap should now be visible. Brass desiccant cap 9. Remove the desiccant cap by turning it counter-clockwise. You can insert a screwdriver in the top of the cap to help loosen the cap. 10.

  • Page 27: Obtaining Technical Support

    Wait 2 hours before starting the imager to allow time for the air inside the camera to dry. Obtaining Technical Support To resolve a problem with your Odyssey XF Imager, start by contacting LI-COR Technical Support at 800-645-4260 (U.S. only), or emailing biohelp@licor.com. Outside of the U.S., contact your local sales office or distributor.
  • Page 28 Page 28...

  • Page 29: Chapter 4: Appendix

    Chemiluminescence none Visible ® Dyes: Optimized for LI-COR® IRDye® 680RD and IRDye 800CW reagents, and chemiluminescent substrates. Image Display Options: Pseudocolor, positive and negative grayscale, single color (red, green or blue), or two colors with overlapping fluorescence displayed in a third color.

  • Page 30: Embedded Firmware Source Code

    Chapter 4: Appendix Weight: 27 kg (60 lbs). The Odyssey XF Imager is certified as a Class 1 laser product. Hazardous laser radiation is not emitted outside the instrument. Radiation emitted inside the Odyssey XF Imager is confined within protective housings and external covers. A series of safety interlocks ensures that laser beams cannot escape during any phase of user operation.

This manual is also suitable for:

2802

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