LI-COR Odyssey M 3340 Operator's Manual
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LI-COR Odyssey M 3340 Operator's Manual

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Odyssey® M
Operator's Manual

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Summary of Contents for LI-COR Odyssey M 3340

  • Page 1 Odyssey® M Operator’s Manual...
  • Page 3 CE Marking: This product (model number 3340, 3350) is a CE-marked product. For conformity information, contact LI-COR Support at http://www.licor.com/biotechsupport. Outside of the U.S., contact your local sales office or distributor. 奥德赛M 成像仪 Odyssey M Imager 部件名称 有毒有害物质或元素 Part Name Toxic and Hazardous Substances or Elements 铅...
  • Page 4 Notes on Safety LI-COR products have been designed to be safe when operated in the manner described in this manual. The safety of this product cannot be guaranteed if the product is used in any other way than is specified in this manual.
  • Page 5 WARRANTY OF ANY KIND WITH REGARD TO THIS MATERIAL, INCLUDING, BUT NOT LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. LI-COR shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing, performance, or use of this material.

  • Page 6: Table Of Contents

    Chapter 2: System Overview External Panels and Controls Front Panel and Scan Surface Rear Panel General Description of Scanning LI-COR Acquisition Software Overview Continuous Operation Disconnecting the Power Optical System Description Brief Description of Illumination Sources Computer Connections and Networking...
  • Page 7 Chapter 3: Operation Before You Begin Clean the Scan Surface Clean Silicone Mat (926-70003) Clean Alignment Guides (926-18972 and 926-18971) Imaging Membranes Handling Membranes Scanning Fluorescent and Colorimetric Membranes Scanning Chemiluminescent Membranes Imaging Gels Scanning Gels Imaging Multiwell Plates Scanning Multiwell Plates Imaging Slides Scanning Slides Obtaining Technical Support...

  • Page 9: Chapter 1: Installation And Safety Information

    Odyssey® Chapter 1: Imager Installation and Safety Information Safety Considerations Intended Use of the Odyssey M Imager The Odyssey M provides reliable data for membranes, plate-based assays, slides, nucleic acid gels, and protein gels. The Odyssey M is versatile and can capture assays in 18 channels, including white light, luminescence (bioluminescence and chemiluminescence), and fluorescence (near-infrared / visible).
  • Page 10 Chapter 1: Installation and Safety Information The Odyssey® M Near-Infrared Imaging System contains four lasers; one emitting at 488 nm, 520 nm, 685 nm, and 785 nm. The lasers emit visible and invisible laser radiation - avoid eye or skin exposure to direct or scattered radiation.
  • Page 11 Odyssey® M Imager Imager Label Locations Figure 1-3. The label affixed to the rear panel of the imager contains ETL, CDRH regulation compliance, and manufacturer information. Figure 1-4. A laser safety label is visible on the front panel of the imager with the Tray Holder removed. Page 11...

  • Page 12: Safety Interlocks

    Chapter 1: Installation and Safety Information Figure 1-5. There is a laser safety label on the bottom panel of the imager. Safety Interlocks The Odyssey® M Imager has safety interlocks that prevent access to the lasers, LEDs, and moving parts, by disabling these hazards when the front or rear lid is opened. Do not attempt to defeat these interlocks.

  • Page 13: Imager Ventilation

    76 cm depth x 38 cm height). With the lid fully open, the Odyssey M has a height of 28" (71 cm). Moving the Imager LI-COR recommends that you do not move your imager. Instead, please contact LI-COR Technical Support for options. LI-COR cannot be held responsible for any damage incurred while moving, transporting, or shipping the Odyssey M Imager, even if an existing warranty and/or service contract applies.

  • Page 14: Leveling The Imager

    Chapter 1: Installation and Safety Information Leveling the Imager Leveling the imager is important for many reasons, including to prevent liquid from pooling in the imaging tray and to keep the imager from tilting. The imager sits on three feet. The two front feet can be used to raise or lower the front corners of the imager.

  • Page 15: Chemical Safety

    Figure 1-8 Turn the metal inserts on the feet to adjust the height. Chemical Safety LI-COR Biosciences recommends that all biochemicals be handled carefully, and that safe laboratory procedures be followed at all times. Be aware of the hazards associated with any chemical before you begin work.

  • Page 16: Fuse Information

      For information about disconnecting power, see Disconnecting the Power on page 35. Fuse Information There are no user-serviceable fuses. If the imager does not power on, please contact LI-COR Biosciences or a LI-COR representative. Routine Maintenance The Odyssey® M requires only minimal maintenance. However, as with any equipment utilizing electrical voltages, there is a danger of fire or electrical shock if the equipment is not properly maintained.

  • Page 17: Clean Or Replace Fan Filter

    The fan filter behind the Tray Holder should be inspected and cleaned at least once a year, but more frequent cleaning may be necessary depending on the cleanliness of the imager's surroundings. If the fan filter becomes damaged, contact LI-COR for a replacement. Follow this procedure when you need to inspect or clean the fan filter:  1.
  • Page 18 Page 18...

  • Page 19: Chapter 2: System Overview

    Power Button Briefly press the Power button  to turn the imager on and off when the imager is not connected to LI-COR® Acquisition Software.   When the imager is powered on, the power button will be illuminated solid blue.
  • Page 20 Chapter 2: System Overview   The username chosen in LI-COR® Acquisition Software when the Odyssey® M Imager was con- nected to LI-COR Acquisition Software.   If the lid is open.   The time remaining in a scan.   During a scan, the type of scan will be shown.
  • Page 21 Odyssey® M Imager Figure 2-3  Move Scan Surface Use the following instructions to move the Scan Surface to the rear of the imager. You may need to do this, for example, to clean the grid pattern below the Scan Surface (see Clean the Grid Below the Scan Surface on page 24).
  • Page 22 Chapter 2: System Overview Manually Move Scan Surface If there is a power outage during a scan, the Scan Surface will not move forward normally. If the Scan Surface is in the rear portion of the imager and is not moving forward normally, you may need to move the Scan Surface forward manually by following these steps.
  • Page 23 Figure 2-5   5) Rotate the thumb screw by hand clockwise to move the Scan Surface forward. If the Scan Surface does not move, please contact LI-COR Technical Support. Do not attempt to move the Scan Surface by applying force to it.
  • Page 24 Chapter 2: System Overview Clean Front Panel To clean the front panel, use mild soap and water. Do not allow soap or water to enter the imager. Remove any debris from the electromagnets on the front two corners of the front lid. Electromagnetic Lid Lock Figure 2-7 ...
  • Page 25 Odyssey® M Imager Clean Underside of Scan Surface Cleaning the underside of the Scan Surface is not part of regular maintenance. The underside of the Scan Surface does not need to be cleaned routinely. Over time, depending on lab conditions, the underside may need to be cleaned.
  • Page 26 Chapter 2: System Overview Balls on bottom of scan glass Figure 2-9 Balls on bottom Figure 2-10 Y-Stage sockets of glass frame Alignment Guides Plate Alignment Guide Use the Plate Alignment Guide (926-18972) to precisely position multiwell plates during imaging. The Plate Alignment Guide is held in position by magnets. Figure 2-11 ...
  • Page 27 Odyssey® M Imager Figure 2-13  Figure 2-14  Sample Enclosure The Sample Enclosure is the frame surrounding the Scan Surface. The Sample Enclosure is held in place by magnets and needs to be removed in some circumstances. Remove the Sample Enclosure Pull upward on the Sample Enclosure to remove it.
  • Page 28 Chapter 2: System Overview Installing the Sample Enclosure Lower the Sample Enclosure into place. The magnets will attract and pull the Sample Enclosure into position. Tray Holder The Tray Holder is used to store the following accessories: the Plate Alignment Guide, the Slide Alignment Guide, and the Silicone Mat.
  • Page 29 Odyssey® M Imager  1) Gently push the door of the Tray Holder, then release. The door will pivot open. Figure 2-18   2) Remove everything inside the Tray Holder.  3) With the door open, push the front of the Tray Holder into the imager. It will move into the imager about 0.5 in.
  • Page 30 Chapter 2: System Overview Figure 2-21   6) Pull the Tray Holder up and away from the base of the imager. The roller pins on the Tray Holder will detach from the hooks on the imager. Figure 2-22  Install Tray Holder  1) Position the lower roller pins on the left and right sides of the Tray Holder into their corresponding hooks.
  • Page 31 Odyssey® M Imager Figure 2-24  Serial Number The serial number for your Odyssey M is located inside the Tray Holder near the top. The serial number is in this format: ODM-XXXX (ODM- followed by four numbers). Serial Number Label Figure 2-25 This image shows the location of the serial number label within the Tray Holder. The Tray Holder has been removed in this photo.

  • Page 32: Rear Panel

    Exhaust: The slotted openings help ventilate air through the imager.   Power IN: Plug the DC power plug part of the LI-COR provided power adapter into this port. Rear Lid The rear lid of the imager can be removed, if necessary, to retrieve samples (see Retrieve Samples on page 34) or to clean the chemi module (Chemi Module Glass on page 35).
  • Page 33 The rear lid is safety interlocked. Removing the lid removes power to the illumination sources and the motors.  1. Exit LI-COR® Acquisition Software  2. Turn off the Odyssey M by pressing the Power button  on the front panel.  3. Disconnect the power cord. See "Disconnecting the Power" on page 35 for more information about disconnecting power.
  • Page 34 Chapter 2: System Overview Rear Lid Latch Figure 2-28   5. Inside the latch hole, press the switch to the left to release the latch. The rear lid will pop up slightly.  6. Lift the rear lid up slightly (about 10 - 20 degrees).  7.

  • Page 35: General Description Of Scanning

    Scans are initiated using LI-COR® Acquisition Software. LI-COR Acquisition Software Overview LI-COR Acquisition Software provides step-by-step workflows for imaging the types of assays that the Odyssey M is intended to image. Choose the appropriate workflow for the type of assay that you want to image, then follow the guidance provided on the pages in the software imaging workflow.
  • Page 36 Chapter 2: System Overview a specific wavelength. This process of light excitation and emission is known as fluorescence. Fluorescence detection can be used for samples or fluorophores. The benefit of using lasers to induce fluorescence is that specific structures (those excited by the wavelength of the incident laser) will be illuminated, but the rest of the sample that does not fluoresce under the indicent laser's wavelength will not be illuminated.

  • Page 37: Computer Connections And Networking

    Note: Use only the supplied Category 6 network cable to connect the Odyssey M and computer. When LI-COR® Acquisition Software starts, it searches for and discovers any Odyssey M Imagers automatically. If only one imager is found, LI-COR Acquisition Software establishes communication.
  • Page 38 Page 38...

  • Page 39: Chapter 3: Operation

    Odyssey® Chapter 3: Imager Operation Before You Begin Clean the Scan Surface thoroughly before and after every scan. Thoroughly clean the silicone mat each time the mat is used. It is very important that the glass and silicone mat be free of smudges, dust, and dye before placing membranes or gels onto the Odyssey M.

  • Page 40: Clean Silicone Mat (926-70003)

    Chapter 3: Operation Metal Frame Scan Surface Figure 3-2  Do not use scouring compounds or abrasive scouring pads. The glass can scratch, which can affect the scanned image.  3) Repeat the wash with 70% ethanol to remove any visible smears. Use methanol to remove any remaining residues.

  • Page 41: Scanning Fluorescent And Colorimetric Membranes

    Odyssey® M Imager Scanning Fluorescent and Colorimetric Membranes  1. Ensure the Odyssey M is powered on and connected to LI-COR® Acquisition Software  2. Open the lid.  3. Place wet or dry membrane(s) on the Scan Surface with the sample-side down.

  • Page 42: Imaging Gels

     4) Place the gel face-up on the Scan Surface, being careful not to trap air bubbles underneath.  5) Close the lid.  6) In LI-COR Acquisition Software, choose the workflow for scanning gels and follow the guidance on the pages in the workflow.

  • Page 43: Scanning Multiwell Plates

    For an In-Cell Western™ Assay, LI-COR recommends the following plates.   For adherent cells, LI-COR recommends a 96-well plate with a clear, flat bottom and black wells, such as the Greiner Bio-One CELLSTAR® Black μClear® Microplate, LI-COR PN 926- 19156 (8 pack) or 926-19157 (32 pack).
  • Page 44  6. Carefully lower the Slide Alignment Guide onto the Scan Surface.  7. Close the lid.  8. In LI-COR® Acquisition Software, connect to the Odyssey® M Imager and start the Microscope Slide image acquisition workflow. Follow the guidance on the pages in the workflow.

  • Page 45: Obtaining Technical Support

    Obtaining Technical Support To resolve a question about your Odyssey M, contact LI-COR Technical Support at 800-645-4260 (North America) or by email at biohelp@licor.com. To help you, Technical Support may request log files or images from LI-COR Acquisition Software. Page 45...
  • Page 46 Page 46...

  • Page 47: Chapter 4: Appendix

    Odyssey® Chapter 4: Imager Appendix Imaging System Specifications Unless otherwise specified, the following apply to Odyssey M Model 3350 and Odyssey M Model 3340. Laser Source The Odyssey M is a Class 1 laser product. Laser Source Maximum Laser Output Beam Divergence 488 nm 30 mW...

  • Page 48: Operating Specifications

    Power Supply: One end of the provided power supply connects to a wall outlet and the other end connects to the imager. The power supply converts from wall outlet voltage to 24 VDC (5 Amp). Only use the provided power supply to plug in the imager. If you need another power supply, contact LI-COR Biosciences. Dimensions  ...
  • Page 49 Odyssey® M Imager Detectors   sCMOS image sensor   Sensor for chemiluminescence (Model 3350): CCD Expected Laser Lifetime   685 nm: 20,000 hours   785 nm: 20,000 hours   488 nm: 40,000 hours   520 nm: 40,000 hours Light Sources  ...

  • Page 50: Odyssey M Detection Channel Summary

    Chapter 4: Appendix Odyssey M Detection Channel Summary Page 50...
  • Page 51 Odyssey® M Imager Odyssey Assay Cat- Excitation / Emission Channel M Chan- egory / Ima- Illumination Filter ging Mode 785 nm 816 - 840 nm 785Ex-820Em Membrane, In-Cell Western (includes On-Cell Western), Cell Analysis, Micro- scope Slide, Custom 685 nm 721 - 740 nm 685Ex-720Em Membrane, Gel, In- Cell Western...

  • Page 52: Embedded Firmware Source Code

    Clear Window 470Trans Gel, ELISA Embedded Firmware Source Code The embedded firmware running on the Odyssey M Imager (model number 3340, 3350) utilizes open source software. To obtain source code or related information, contact LI-COR Support at http://www.licor.com/biotechsupport. Page 52...

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