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CE Marking: This product (model number 5700-DS) is a CE-marked product. For conformity information, contact LI-COR Support at http://www.licor.com/biotechsupport. Outside of the U.S., contact your local sales office or distributor.
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Notes on Safety LI-COR products have been designed to be safe when operated in the manner described in this manual. The safety of this product cannot be guaranteed if the product is used in any other way than is specified in this manual. The Pearl Docking Station and Pearl Clean Box were intended to be used by qualified personnel.
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WARRANTY OF ANY KIND WITH REGARD TO THIS MATERIAL, INCLUDING, BUT NOT LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. LI-COR shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing, performance, or use of this material.
Table of Contents Chapter 1: Pearl Docking Station Description Assembling the Pearl Docking Station Docking Station Operation Routine Maintenance Docking Station Specifications Chapter 2: Pearl Clean Box Description Installation Connecting a HEPA Filter to the Docking Station Connecting a HEPA Filter to the Pearl® Imager Nose Cone Installation and Replacement Sanitizing the Pearl Clean Box Clean Box Operation...
Chapter 1: Pearl Docking Station Description The Pearl Docking Station is an optional, external accessory that duplicates many of the ® features of the Pearl Imager imaging drawer. The main features and functions of the Docking Station are listed below. Duplicates the docking hardware in the Pearl Imager imaging drawer, allowing an accessory imaging bed to be locked in place...
Chapter 1: Pearl Docking Station Assembling the Pearl Docking Station WARNING: Isoflurane gas can leak in the amount supplied to the Pearl Imaging System Docking Station if the product is not used properly. Carefully follow the instructions in this chapter to ensure that the scavenging charcoal filter and user-supplied anesthesia system are properly connected, and that the nose cone on the imaging bed has a nose cone plug inserted when not in use.
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Pearl® Imager Accessories 3) Connect the tube from the port labeled “OUT” on the rotameter to the port labeled “IN” on the back of the docking station. The gender of the connector prevents connection to the wrong port. Figure 1-3. Docking station back panel. 4) Connect the tube from the port labeled “EXHAUST”...
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Chapter 1: Pearl Docking Station 6) Connect one of the provided HEPA filter assemblies between the SmartFlow system and the Induction Chamber. 7) Connect the opposite end of the HEPA filter assembly to the provided anesthesia gas supply tube and then connect the other end of the anesthesia gas supply tube to one of the output manifold connectors on the SmartFlow Anesthesia System, or equivalent (Figure 1-5).
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Pearl® Imager Accessories 8) Hold the base of the rotameter stand and press the charcoal filter down onto the port labeled “EXHAUST” on the exterior of the rotameter. Alternatively, connect a user-supplied charcoal fil- ter assembly to the EXHAUST port. Recommendations for monitoring and replacement of the charcoal filter are given in Chapter 3 of the Pearl Imager Operator's Manual.
Chapter 1: Pearl Docking Station Docking Station Operation 1) Install a nose cone plug in the nose cone of the accessory imaging bed (Figure 1-8) and install ® the imaging bed on the Pearl Imaging System Docking Station. Lock the imaging bed in place using the guide pins and locking handle as described in Chapter 2.
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(bent), or need replacement. See Chapter 5 of the Pearl Imager Operator's Manual for the contact pin replacement procedure. Contact LI-COR if the error condition persists after contact pin replacement. 3) Wait for the heater plate to reach the set point (38 °C). The green “running” icon blinks rapidly until the set point is reached and then stops blinking and remains on (Figure 1-9).
Chapter 1: Pearl Docking Station 7) After imaging, remove the accessory imaging bed, lock it back on the docking station, remove the mouse to a recovery chamber, and re-insert the nose cone plug to stop the flow of anesthesia gas. Turn off the flow of anesthesia gas after imaging the last mouse. Routine ...
Chapter 2: Pearl Clean Box Description The purpose of the Pearl Imager Clean Box is to provide a HEPA-filtered closed environment for mice requiring added protection from exposure to pathogens. The lower portion of the Clean Box is an imaging bed with a heater plate similar to the standard Pearl imaging bed.
The nose cone may need to be replaced occasionally for sanitary or other reasons. New nose cones can be purchased from LI-COR. The nose cone can be removed by grasping the tip and pulling it straight off (parallel to the imaging bed surface) until the nose cone slips off its base.
If necessary, perform a final cleaning using the ® LI-COR Optical Tissue. Smudges on the window could be visible in all images acquired using the Clean Box, so clean thoroughly and carefully.
Chapter 2: Pearl Clean Box 3) Turn on the docking station and wait for the heater plate to reach the temperature set point (green “running” icon is ON constantly). Note: Alternatively, the heater plate can be warmed by locking the Clean Box in the Pearl ima- ging drawer until the set point temperature is reached (see the Pearl Imager Operator's Manual).
Follow with a second dry tissue to remove any residual liquid or streaks. For heavy duty cleaning of the window or entire Clean Box, use durable Heavy Duty Optical Wipes. A final cleaning of the window surface using the LI-COR Premium Grade Optical Tissue, as discussed above, may be required.
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Siemensstraße 25A • 61352 Bad Homburg • Germany Phone: +49 (0) 6172 17 17 771 • Fax: +49 (0) 6172 17 17 799 • bio-eu@licor.com LI-COR Biosciences UK Ltd. St. John’s Innovation Centre • Cowley Road Cambridge • CB4 0WS United Kingdom •...
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