Nikon ECLIPSE E400 Instructions Manual page 19

© Use as little oil as possible (just enough to fill the space between the tip of the objective and the specimen, or between the tip
of the condenser and the specimen). If too much oil is applied, the excess will flow onto the stage and around the condenser.
© Any oil remaining on oil-immersion type objectives or on the tip of dry-type objectives has a negative effect on viewing.
After using oil, wipe all of it away and make sure that there is no oil on the tips of the other objectives. The oil on the
condenser lens should also be carefully wiped away after use.
@ Use petroleum benzine to wipe away immersion oil. Finish the cleanup by wiping with absolute alcohol (ethyl alcohol or
methyl alcohol).
© If you cannot obtain petroleum benzine, use methyl! alcohol. But it will be necessary to wipe the surfaces repeatedly (usually,
three or four times) to clean the lenses.
WARNING
@ When using petroleum benzine or absolute alcohol, please follow the instructions provided by the manufacturer.
Keep these flammable liquids away from fire or sparks.
— Precautions when using immersion oil
@ Make sure to securely tighten the cap after use. After adding oil to the container, securely tighten the base cap.
Since the base cap may become loose by itself after having been used for a long time, periodically check to make
sure it is tight. Oil may leak out if the cap is loose.
@ Do not press on the container with excessive force. This may cause the oil suddenly spray from the container.
© Wipe off any oil that collects on the outside of the container during the course of use.
O Be careful so that oil does not get in your eyes. Although the oil is not toxic, apply the first-aid measures listed
below if it should come in contact with the skin or enter the eyes.
© Contact with skin: Completely wash off with soap and water.
© Enters the eyes: Rinse thoroughly with pure water (for at least 15 minutes) and seek medical attention.
O Do not place immersion oil in a location exposed to outside light (or UV light).
© Water-immersion operation
- (using a water-immersion type objective and condenser)
O Objectives marked "WI" or "W" are water-immersion type objectives. These objectives are used with the immersion water
(distilled water or physiological saline) applied between the specimen and objective. Microscopy is performed in the same
manner as for an oil-immersion type objective.
@ Since water evaporates easily, periodically check it during observation. Applying excess water will cause it to flow onto the
stage and condenser resulting in corrosion.
@ After use, wipe any water from the tip of the objective and finish the cleanup process by wiping with absolute alcohol.
@ When the water dries and leaves a stain, apply a small amount of neutral detergent and wipe gently to remove the stain.
Finish the cleanup process by wiping with absolute alcohol.