Nikon ECLIPSE E400 POL Instructions Manual page 25

Polarizing microscope
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3. Operation of Each Part
Use of optional accessories (Sold Separately)
1. P-CS Sénarmont Compensator
The P-CS Sénarmont compensator is used by inserting
into the slot of the intermediate tube in place of the
P-CL 1/4, & tint plate.
It can be used to measure retardation up to 1A according
to the following procedure.
1
Determination of Extinction Position
2
Determination of Subtraction Position
Rotate the stage 45° from the extinction position to the diagonal position. Insert the P-CL
1/4 & tint plate into the optical path and confirm that the interference color of the section
of the specimen to be measured changes toward the lower order side. If the interference
color changes toward the higher order side, rotate the stage another 90°.
3
Measurement
Place the GIF filter on the field lens and replace the P-CL 1/4) & tint plate with the P-CS
Sénarmont compensator. Rotate the analyzer so that the section of the specimen to be
measured becomes darkest. When the rotation angle of the analyzer at that time is taken to
be theta (6) degrees, then retardation (R) (nm) is determined with the following formula:
8
.
R=
180
А
(A; wavelength used)
The value of À when using the GIF filter is 546 nm.
25

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