Nikon ECLIPSE E800 Instructions Manual page 8

3. Microscopic Procedure
Switch to any desired objective
and view the specimen.
• Re-adjust the focus.
• Use ND filters to adjust the
brightness.
• Close the field diaphragm so
that it is just outside the field
of view.
• Close the condenser aperture
diaphragm to about 70 to
80% of the objective
numerical aperture, (p. 19)
■ If a portion of the field of view is dark, try adjusting the condenser centering screws. If that does
not resolve the problem, check the following items:
• Filter insertion/removal (p. 21)
• Positioning of the revolving nosepiece (p. 35)
• Condenser focusing (p. 18)
• Full opening of the field and aperturediaphragms (p. 19, 20)
• Optical path selection for the eyepiece tube (p. 16)
• Lamp installation (p. 28)
• Revolving nosepiece installation (p. 30)
• Condenser installation (p. 30)
■ If it is not possible to focus on the specimen, check the following items.
• Mounting of the specimen (p. 35, 36)
• Release of the coarse focus stopper (p. 15)
• Thickness of the cover glass (0.17 mm is standard) (p. 35)