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Related Manuals for Biomeme Franklin three9 ISP
Summary of Contents for Biomeme Franklin three9 ISP
- Page 1 Franklin three9 ™ BW Panel Playbook Last Updated: 11/10/2021 Version 1.3...
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Page 2: Table Of Contents
3. Insert Cartridge Into Thermocycler and Start Run Interpreting Results Interpretation Table Detailed Data View Transferring Data Wired (USB) Wireless (Bluetooth) Recovering & Reattaching Test Data Common Scenarios Common Questions three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020 Version 1.3... -
Page 3: Bw Panel Overview
Scenario B Scenario C Scenario D Storage Troubleshooting Frequently Asked Questions (FAQ) Failure Modes Disclaimer BW Panel Overview For Research Use Only. The Biomeme BW Panel is an end-to-end mobile three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020 Version 1.3... - Page 4 Biomeme Franklin™ three9 ISP (Integrated Sample Prep) qPCR thermocycler. Safety Warning: When working with our products, always wear appropriate personal protective equipment (PPE) (e.g. lab coat, disposable gloves with adequate chemical resistance, mouth/face protection, goggles, etc.) For more information, please review the product’s safety data sheet(s) (SDS).
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Page 5: Thermocycler Kit Components
(Pouch contains cartridge and transfer pipette) Note: You only need 1 pouch to run a test. You can choose from BW Panel, BW Simulant Panel, or BW Training Panel. three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020 Version 1.3... -
Page 6: Targets
Orthopox viruses Pox2 genome variola genome Ric1 ricin toxin gene Ricin Ric2 ricin toxin gene Ric3 ricin toxin gene Staphylococcal SEB1 SEB toxin gene enterotoxin B SEB2 SEB toxin gene three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020 Version 1.3... -
Page 7: Bw Simulant Panel
IPC3 synthetic DNA control sequence Note: There are 9 wells per test cartridge. Wells 4-9 of the BW Simulant Panel cartridge are empty and do not test for anything. three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020 Version 1.3... -
Page 8: Bw Training Panel
Loads 1 of the 3 protocols listed Random Result See Training Cartridge Pouch below at random Amplifies IPC, MS2, Ba1, Ba2, and Ba3 targets only Detected Amplifies IPC and MS2 targets only Not Detected three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020 Version 1.3... -
Page 9: Thermocycler Button Layout
Displays run error with instructions to scan a new test Alert Thermocycler Button Layout There are a total of 4 buttons located on the top of your Franklin™ three9 ISP thermocycler: three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020 Version 1.3... -
Page 10: Led Status Indicators
Note: If the battery is in the red, the device will not start a run until it has been charged to 30%. 5 blinking indicates your test is complete and data is ready to BLUE be transferred to your smartphone three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020 Version 1.3... -
Page 11: Thermocycling Protocol
1 minute @ 95° C 1 minute @ 95° C Denature 8 seconds @ 98° C 3 seconds @ 95° C Anneal 15 seconds @ 60° C 15 seconds @ 60° C three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020 Version 1.3... -
Page 12: Overall Workflow
Overall Workflow three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020 Version 1.3... -
Page 13: How To Run A Test
Follow the simple steps outlined below to begin your test. Open the Biomeme BWGo app on your smartphone by tapping the icon on your phone’s home screen if you haven’t already. From the main home screen of Biomeme BWGo, tap Start Run. - Page 14 Once Bluetooth is enabled on the thermocycler, tap Connect via BLE when prompted within the smartphone app. If working with multiple Franklin™ three9 ISP thermocyclers, the app will list each thermocycler’s MAC address. Select the appropriate unit and tap Confirm. three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020 Version 1.3...
- Page 15 If done incorrectly, you risk damaging the thermocycler. Insert the short USB cable into the smartphone. Then connect the two cables. Tap OK in the pop-up in the smartphone app. three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020 Version 1.3...
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Page 16: Add Sample To Test Cartridge
2. Add Sample to Test Cartridge Each pouch contains 1 Biomeme test cartridge. Pay close attention to the labels on the pouch to differentiate between them. Tear open the foil pouch to retrieve your cartridge and pipette, but DO NOT discard the foil pouch as you’ll later need to scan the QR code found on the pouch label. -
Page 17: Insert Cartridge Into Thermocycler And Start Run
ISP cartridge should not be removed until the run is complete. If you haven’t already, launch your Biomeme smartphone app, tap Start Run and follow the instructions in app. three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020... - Page 18 If the app freezes or crashes, the experiment will also continue to run and your data can be found in the Incomplete Runs section of the app once you’ve reopened the Biomeme BWGo app and reconnected to the thermocycler.
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Page 19: Interpreting Results
No pathogen targets and at least some Alert IPCs did not amplify. Note: All BW Training Panel results will display text that identify the result as a training result. three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020 Version 1.3... -
Page 20: Detailed Data View
Target baselined PCR amplification PCR amplification plot(s) and ID and Cq value. plot(s), and Cq value(s). see Cq value(s). three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020 Version 1.3... -
Page 21: Transferring Data
USB-A end of the cable into the computer. On the smartphone, swipe down from the top of the screen and tap "Use USB for Charging" then select file transfers. three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020 Version 1.3... -
Page 22: Wireless (Bluetooth)
Wireless (Bluetooth) On your computer, make sure your Bluetooth is set to Receive a File. This will prepare your computer to accept the data transfer from your smartphone. three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020 Version 1.3... -
Page 23: Recovering & Reattaching Test Data
In the Biomeme mobile app, navigate through View Results and select a test. Once on the test result screen in the app, tap Send in the top right corner. A menu will slide in with sharing options. Select Bluetooth and transition to the Choose Bluetooth Device screen. -
Page 24: Common Questions
Scenario A My thermocycler is ON, my test is currently running or has recently completed, the thermocycler’s front LED lights are flashing blue, and my Biomeme smartphone app is still OPEN. 1. Power cycle the Bluetooth. Press the Bluetooth button on top of the thermocycler to turn OFF the Bluetooth Pairing. -
Page 25: Scenario B
Scenario B My thermocycler is ON, my test is currently running or has recently completed, but my Biomeme smartphone app has CLOSED. 1. Relaunch the Biomeme smartphone app by selecting the icon on your smartphone’s home screen. 2. Press the Bluetooth button on top of the thermocycler to turn it OFF the bluetooth. -
Page 26: Scenario C
Scenario C My thermocycler is OFF but my Biomeme smartphone app is OPEN. 1. Press the Power button on top of your Franklin™ three9 ISP thermocycler to turn it back on. If your thermocycler doesn’t turn back on, make sure it’s connected to a power source as your battery may have died. -
Page 27: Storage
4. Press the Bluetooth button on top of your thermocycler. 5. Relaunch the Biomeme smartphone app by selecting the icon on your smartphone’s home screen. 6. From the Biomeme app’s home screen, tap Incomplete Runs. 7. Select your test from the list of incomplete runs. -
Page 28: Troubleshooting
It will prompt you to reconnect to the thermocycler if you are able to. Upon reconnecting, the test data will update on the smartphone after a short delay (1-2 seconds). three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020 Version 1.3... - Page 29 If your test fails, the smartphone will notify you of the error returned from the thermocycler. Your last run will be saved in the Biomeme mobile app up to the point of failure, but if your run failed or stopped with less than 20 cycles, the data will not be processed so no Cq values will be calculated and the PCR amp plot data will not be baselined or smoothed.
- Page 30 What should I do if I receive an error message? Retry running your test, but if the error persists please contact support@biomeme.com. three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020 Version 1.3...
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Page 31: Failure Modes
● Turn OFF Bluetooth on your smartphone. Then, turn Bluetooth back ON and attempt to reconnect to the thermocycler. ● Close the Biomeme mobile app completely, then relaunch it and attempt to access Incomplete Runs from the app home screen to recover your test results. - Page 32 The LEDs will remain on the progress bars until the flush is completed, then move to blinking red. Once the LEDs are blinking red, it is safe to open the lid. three9™ ISP BW Panel Playbook © Biomeme, Inc. 2020 Version 1.3...
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Page 33: Disclaimer
For Research Use Only. Not for use in human or veterinary diagnostics. The performance characteristics of this product have not been established. Biomeme products may not be transferred to third parties, resold, modified for resale or used to manufacture commercial products or to provide a service to third parties without written approval of Biomeme, Inc.
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