Biomeme Franklin three9 User Manual

Biomeme Franklin three9 User Manual

Isp real-time pcr thermocycler
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Franklin™ three9 
ISP Real-Time PCR 
Thermocycler 
 
 
 
Last Updated​ : 03/05/2020 
 
 

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Summary of Contents for Biomeme Franklin three9

  • Page 1           Franklin™ three9  ISP Real-Time PCR  Thermocycler        Last Updated​ : 03/05/2020     ...
  • Page 2: Table Of Contents

    Transferring Data 13  Recovering & Reattaching Test Data 15  Maintenance & Cleaning 19  Safety Notice 20  General Safety Warnings 20  Troubleshooting 22  Disclaimer 25      Franklin™ three9 ISP PCR Thermocycler User  © Biomeme, Inc. 2020  Version 1.0  Manual   ...
  • Page 3 At just over 4 lbs, the hand-held and internally battery-operated system  provides for maximum portability while enabling a full day’s work out in the field  on a single charge.        Franklin™ three9 ISP PCR Thermocycler User  © Biomeme, Inc. 2020  Version 1.0  Manual   ...
  • Page 4: Technical Specifications

    Wired (USB micro)  Yes  Integrated Barcode Scanner  (via smartphone app)  Max Samples per Run  1  Max PCR Targets per Run  27  Weight  1.95kg / 4.30 lb    Franklin™ three9 ISP PCR Thermocycler User  © Biomeme, Inc. 2020  Version 1.0  Manual   ...
  • Page 5 Note ​ : Transport your Franklin™ three9 ISP thermocycler in its carrying case. Moving your thermocycler while  thermocycling could result in errors. We highly recommend not moving or opening the device while thermocycling to    avoid losing your PCR run.    Franklin™ three9 ISP PCR Thermocycler User  © Biomeme, Inc. 2020  Version 1.0  Manual   ...
  • Page 6: Thermocycler Button Layout

    Thermocycler Button Layout    There are a total of 4 buttons located on the top of your Franklin™ three9 ISP  thermocylcer:                Franklin™ three9 ISP PCR Thermocycler User  © Biomeme, Inc. 2020  Version 1.0  Manual   ...
  • Page 7: Led Status Indicators

        5 blinking indicates your test is complete and data is ready to  be synced to your smartphone          Franklin™ three9 ISP PCR Thermocycler User  © Biomeme, Inc. 2020  Version 1.0  Manual   ...
  • Page 8: Turning Your Thermocycler On And Off

    The LED  on the back of the thermocycler will illuminate blue. If you're unable to turn your    Franklin™ three9 ISP PCR Thermocycler User  © Biomeme, Inc. 2020  Version 1.0  Manual   ...
  • Page 9 If your device has  not been charged in more than six months and you cannot get the thermocycler to  turn back on, please contact ​ upport@biomeme.com         Franklin™ three9 ISP PCR Thermocycler User  © Biomeme, Inc. 2020  Version 1.0  Manual   ...
  • Page 10: Enabling & Disabling Bluetooth (Ble )

    1. Tap ​ C onnect via USB​ on the smartphone app.    2. Press Bluetooth button on top of the device and tap ​ C onfirm​ in the  smartphone app.    Franklin™ three9 ISP PCR Thermocycler User  © Biomeme, Inc. 2020  Version 1.0  Manual   ...
  • Page 11: Loading Sample Into Isp Cartridge

      5. Tap ​ O K​ in the pop-up in the smartphone app.              Loading Sample into ISP Cartridge      Franklin™ three9 ISP PCR Thermocycler User  © Biomeme, Inc. 2020  Version 1.0  Manual   ...
  • Page 12: Placing Cartridge Into Franklin™ Three9 Isp

    ISP    ● Open the lid of your Franklin™ three9 ISP thermocycler by sliding the latch  on the front of the unit to the right.    Franklin™ three9 ISP PCR Thermocycler User  © Biomeme, Inc. 2020  Version 1.0  Manual   ...
  • Page 13: Transferring Data

    13    ● Insert your ISP cartridge and close thermocycler lid.  ● If you haven’t already, launch your Biomeme smartphone app, tap ​ S tart Run  and follow the instructions in the app.  For further instructional information, please contact ​...
  • Page 14 Exact steps for transferring data from your smartphone to your  computer could vary depending on your operating system. If you require further assistance, please contact  support@biomeme.com ​ .     Wireless (Bluetooth)    Franklin™ three9 ISP PCR Thermocycler User  © Biomeme, Inc. 2020  Version 1.0  Manual   ...
  • Page 15: Recovering & Reattaching Test Data

      2. In the Biomeme mobile app, navigate through ​ V iew Results​ and select a test.    3. Once on the test result screen in the app, tap ​ S end​ in the top right corner. ...
  • Page 16 “My run has completed and I’ve pressed a bunch of buttons. I’m feeling flustered,    confused, and frustrated; what should I do?” Turn off the thermocycler and close the Biomeme smartphone app.  Follow the steps described in section ​ ​ below. ...
  • Page 17   A: “My thermocycler is ​ O N ​ , my test is currently running or has recently completed  and the LED lights are flashing blue, and my Biomeme smartphone app is still  OPEN ​ . ”  1. Power cycle the Bluetooth. Press the Bluetooth button on top of the ...
  • Page 18 9. Please wait while your run data is transferred.    C: “My thermocycler is ​ O FF ​ but my Biomeme smartphone app is ​ O PEN ​ . ”  1. Press the Power button on top of your Franklin™ three9 ISP ...
  • Page 19     19  D: “My thermocycler is ​ O FF ​ and my Biomeme smartphone app is ​ C LOSED ​ . ”   1. Press the Power button on top of your Franklin™ three9 ISP  thermocycler to turn it back ​ O N​ . If your thermocycler doesn’t turn back on, ...
  • Page 20: Maintenance & Cleaning

    20      Maintenance & Cleaning    The Biomeme Franklin™ three9 ISP thermocycler is maintenance-free and has no  serviceable parts. In the case of thermocycler failure or damage, please contact  support@biomeme.com​ .     The outside of the Franklin™ three9 ISP thermocycler can be cleaned using 70% ...
  • Page 21: General Safety Warnings

        Electrical Safety Warnings    Unplug AC power cord from the wall outlet in case of an  emergency.          SYMBOL  DESCRIPTION   Franklin™ three9 ISP PCR Thermocycler User  © Biomeme, Inc. 2020  Version 1.0  Manual   ...
  • Page 22: Troubleshooting

    Why is my Bluetooth (BLE) not connecting?    If you are having trouble connecting, ensure that you enabled Bluetooth and  “Location Services” on your smartphone and Bluetooth on your thermocycler.      Franklin™ three9 ISP PCR Thermocycler User  © Biomeme, Inc. 2020  Version 1.0  Manual   ...
  • Page 23 If your test fails, the smartphone will notify you of the error returned from the  thermocycler. Your last run will be saved in the Biomeme mobile app up to the  point of failure, but if your run failed or stopped with less than 20 cycles, the data ...
  • Page 24 If the smartphone app closes during a test, you can sync to the test by reopening  the app and pressing the ​ I ncomplete Runs​ option from the Biomeme app home  screen. This will display the previous incomplete run. See Recovering & ...
  • Page 25 ● Turn OFF Bluetooth on your smartphone. Then, turn Bluetooth back ON and  attempt to reconnect to the thermocycler.  ● Close the Biomeme mobile app completely then relaunch it and attempt to  access ​ I ncomplete Runs​ from the app home screen to recover your test ...
  • Page 26: Disclaimer

      Biomeme products may not be transferred to third parties, resold, modified for  resale or used to manufacture commercial products or to provide a service to  third parties without written approval of Biomeme, Inc. ...

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