Biomeme Franklin User Manual
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Biomeme Franklin User Manual

Real-time pcr thermocycler
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Franklin™ 
Real-Time PCR 
Thermocycler and 
Biomeme Go App 
 
Last Updated​ : 03/13/2020 
Version​ : 1.0 
 
 
 
 

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Summary of Contents for Biomeme Franklin

  • Page 1         Franklin™  Real-Time PCR  Thermocycler and  Biomeme Go App      Last Updated​ : 03/13/2020  Version​ : 1.0     ...

  • Page 2: Table Of Contents

        Brief Overview 4  Thermocycler Technical Specifications 5  Thermocycler Button Layout 7  Logging In and Out of Biomeme Go 8  Loading Sample into Go-Strips 8  Placing into Franklin™ Thermocycler 9  How To Start Your First Run 10  During the Run 13 ...
  • Page 3     3  Charging & Checking Thermocycler Battery Status 21  Enabling & Disabling Bluetooth (BLE ) on Your Thermocycler 23  How To Update the Biomeme Go App 23  How To Run a Melt Curve 24  Transferring Data 25  Wireless (Bluetooth) 25 ...

  • Page 4: Brief Overview

    27 targets from 1 sample or test 9 samples for up to 3  targets each. Just under 3 lbs, hand-held, and battery-operated for maximum  portability enabling a full day’s work out in the field on a single charge. Franklin™  comes in 3 different variations depending on the color channel detection you ...

  • Page 5: Thermocycler Technical Specifications

    ATTO647N (Red)  Wireless (BLE)  System Control & Data Transfer  Integrated Barcode Scanner  Yes  Max Samples per Run  9  Max PCR Targets per Run  27    Franklin™ PCR Thermocycler and Biomeme Go  © Biomeme, Inc. 2020  Version 1.0  User Manual   ...
  • Page 6 Full Load Current  3.3A  Internal Battery  5 hrs  Quantitative  Yes  IP Rating  IP30  Indoor/Outdoor?  Indoor or Outdoor in a Covered Area  Pollution Degree  2    Franklin™ PCR Thermocycler and Biomeme Go  © Biomeme, Inc. 2020  Version 1.0  User Manual   ...

  • Page 7: Thermocycler Button Layout

    Thermocycler for Proper Performance    Note: ​ T he Franklin™ one9 is limited to the FAM/SYBR (Green) channel and the two9 is limited to the FAM/SYBR (Green)  and ATTO647N (Red) channels only. You can ​ u pgrade your thermocycler(s) ​...

  • Page 8: Logging In And Out Of Biomeme Go

      To log in (Requires internet connection)    Open the Biomeme Go mobile app on your smartphone by tapping the app  icon on your phone’s home screen.  Enter your email address and password (both are case sensitive).  Tap the ​ L ogin​ button. ...

  • Page 9: Placing Into Franklin™ Thermocycler

    When the lid of the  thermocycler is closed, it will secure the caps into place, sealing the PCR  reaction.    Franklin™ PCR Thermocycler and Biomeme Go  © Biomeme, Inc. 2020  Version 1.0  User Manual ...

  • Page 10: How To Start Your First Run

    .     Note: ​ Transport your Franklin™ thermocycler in its carrying case. Additionally, moving your thermocycler while  thermocycling could result in errors. We highly recommend not moving or opening the device while thermocycling to  avoid losing your PCR run. After your run has completed, be careful when removing your Go-Strips and void filling caps ...
  • Page 11 (subtract) icons, then tap  Confirm​ . The maximum number of Go-Strips per test run is 3.  Biomeme’s Go-Strips are highly adaptable to meet your individual needs,  so choose the run layout that fits your test best.  Choose to ​ S can​ or ​ G enerate​ your Sample IDs. You can change these on the ...
  • Page 12   Note: ​ T he first time you to try scan for devices, you’ll be asked to give the Biomeme Go app permission to  turn on Bluetooth. Please make sure that the “Location” service is enabled in your phone settings. The latest ...

  • Page 13: During The Run

    If the app freezes or crashes, the experiment will also continue to run and your data can  be found in the Incomplete Runs section of the app once you’ve reloaded the Biomeme Go app and  reconnected to the thermocycler. For more information on Incomplete Runs, please refer to the “ ​...

  • Page 14: How To Create A New Protocol

        How To Create a New Protocol    Log into the Biomeme Go app if you haven’t done so already (see “​ L ogging  In and Out​ ” ).  Select ​ P rotocol Management​ from the main dashboard of Biomeme Go. ...

  • Page 15: Interpreting Results

    Note: ​ T he colors, statements, and Cq values used in the screenshots below are only examples and your results interface    may vary. Please consult your Team Admin for details regarding the Cq ranges for your team’s protocol(s).   Franklin™ PCR Thermocycler and Biomeme Go  © Biomeme, Inc. 2020  Version 1.0  User Manual ...
  • Page 16 View Cq values for each of your  targets.   Baselined Data  View amplification plots for your  baselined data.  Raw Data  View amplification plots for your raw  data.        Franklin™ PCR Thermocycler and Biomeme Go  © Biomeme, Inc. 2020  Version 1.0  User Manual   ...
  • Page 17     17    Baselined Data Example  Raw Data Example                    Franklin™ PCR Thermocycler and Biomeme Go  © Biomeme, Inc. 2020  Version 1.0  User Manual   ...
  • Page 18 Background color based on your test  ranges (e.g. 1 bar = Low, 2 bars =    result (e.g. green, yellow, red).   Medium, 3 bars = High).   Franklin™ PCR Thermocycler and Biomeme Go  © Biomeme, Inc. 2020  Version 1.0  User Manual   ...

  • Page 19: Viewing Completed Run Data

    In and Out​ ” ).  Select ​ I ncomplete Runs​ from the main dashboard of Biomeme Go to view a  list of all runs classified as incomplete.  Note: ​ A fter selecting your incomplete run, please wait roughly 30 seconds before your run data begins to populate. Be ...

  • Page 20: Thermocycler Led Status Indicators

    Thermocycler LED Status Indicators    Vertical LED on the front of your thermocycler.    Franklin™ has 5 LEDs on the front of the unit. The LEDs are used to convey various  states of the thermocycler as outlined in the table below.     ...

  • Page 21: Turning Your Thermocycler On And Off

    If your battery is running low, simply plug the AC power adapter into an outlet and  insert the power connector into the back of your thermocycler. The LED on the  back of the thermocycler will illuminate blue.       Franklin™ PCR Thermocycler and Biomeme Go  © Biomeme, Inc. 2020  Version 1.0  User Manual   ...
  • Page 22 If your device has not been  charged in more than six months and you cannot get the thermocycler to turn back  on, please contact ​ upport@biomeme.com     Franklin™ PCR Thermocycler and Biomeme Go  © Biomeme, Inc. 2020  Version 1.0  User Manual   ...

  • Page 23: Enabling & Disabling Bluetooth (Ble ) On Your Thermocycler

    Once enabled, tap ​ C onnect via BLE​ when prompted in the smartphone app. If  working with multiple Franklin™ thermocyclers, select the appropriate unit and tap  Confirm​ . The LED on the front of your thermocycler will flash white indicating it’s ...

  • Page 24: How To Run A Melt Curve

      Note: ​ P CR and Melt Curves are separate runs in Biomeme Go. After your PCR finishes, create a new Melt Curve run  (following the steps above). The PCR and Melt Curve data will be stored as separate runs within the app and to ...

  • Page 25: Transferring Data

      Reporting a Problem or Error    Log into the Biomeme Go app if you haven’t done so already (see “Logging  In and Out”).  Tap the Gear icon in the top right corner to open the ​ S ettings​ menu. ...

  • Page 26: Viewing Thermocycler Device Information

        Viewing Thermocycler Device  Information in Biomeme Go    Log into the Biomeme Go app if you haven’t done so already (see “​ L ogging  In and Out​ ” ).  Tap the Gear icon in the top right corner to open the ​ S ettings​ menu. ...

  • Page 27: Maintenance & Cleaning

      The outside of the Franklin™ thermocycler can be cleaned using 70% ethanol  solution which must be sprayed on a cloth rather than directly on the Franklin  thermocycler. Lysol wipes or Micro-Chem Plus wet paper towels are acceptable as  well. Do not spray or pour solution directly onto the thermocycler when cleaning. ...

  • Page 28: Safety Notice

    Electrical Safety Notice.    CAUTION​ - Heating wells may be hot. Care must be taken when  inserting or removing cuvettes.          Franklin™ PCR Thermocycler and Biomeme Go  © Biomeme, Inc. 2020  Version 1.0  User Manual   ...
  • Page 29     Franklin™ PCR Thermocycler and Biomeme Go  © Biomeme, Inc. 2020  Version 1.0  User Manual   ...

  • Page 30: Declaration Of Conformity

    ETSI EN 301 489-17 V3.1.1     Hazardous Substances: EN 50581:2012   This declaration of conformity is issued under the sole responsibility of the  manufacturer.          Franklin™ PCR Thermocycler and Biomeme Go  © Biomeme, Inc. 2020  Version 1.0  User Manual   ...

  • Page 31: Troubleshooting

    “My run has completed and I’ve pressed a bunch of buttons. I’m feeling flustered,  confused, and frustrated; what should I do?”  Turn off the thermocycler and close the Biomeme smartphone app.  Follow the steps described in section ​ ​ below. ...
  • Page 32 B: “My thermocycler is ​ O N ​ , my test is currently running or has completed, and my  Biomeme Go app has ​ C LOSED ​ . ”  1. Relaunch the Biomeme smartphone app by selecting the icon on your  smartphone’s home screen. ...
  • Page 33 9. Please wait while your run data is transferred.    C: “My thermocycler is ​ O FF ​ and my Biomeme Go app is ​ O PEN ​ . ”  1. Press the Power button on top of your Franklin™ three9 thermocycler to ...
  • Page 34     34  D: “My thermocycler is ​ O FF ​ and my Biomeme Go app is ​ CLOSED ​ . ”  1. Press the Power button on top of your Franklin™ three9 thermocycler to  turn it back ​ O N​ . If your thermocycler doesn’t turn back on, make sure it’s ...
  • Page 35   You must first pair your thermocycler and smartphone. Then...    1. From the Biomeme Go main dashboard, tap the gear icon in the upper right  corner.  2. Select ​ S ettings​ .   3. Tap the text field with the existing Bluetooth name and override it with a ...
  • Page 36 If your test fails, the smartphone will notify you of the error returned from the  thermocycler. Your last run will be saved in the Biomeme mobile app up to the  point of failure, but the data will not be processed resulting in no Cq values, ...
  • Page 37 TexasRedX (Amber). Once your order is received, a Biomeme representative will  follow up regarding your MAC address(es) and use this to provide you with a  unique 16-character unlock code (per unit) to be entered using the Biomeme  mobile app. Please note, your smartphone must be connected to the thermocycler ...

  • Page 38: Disclaimer

      Biomeme products may not be transferred to third parties, resold, modified for  resale or used to manufacture commercial products or to provide a service to  third parties without written approval of Biomeme, Inc. ...

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