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Summary of Contents for Leica TCS SP
- Page 1 True Confocal Scanner Leica TCS SP User Manual...
- Page 2 LCS File Formats..........................45 Specification of the "Lei" File Format (Version beta 2.000) .............47 General ..............................54 Introduction to the Leica Confocal Software Help ................55 Opening the Context-Sensitive Help ....................55 Procedures and Exercises for Selected Processes ................63 Setting Instrument Parameters for the First Image Recording............63 Creating User-Defined Instrument Parameter Settings (IPS) ............69...
- Page 3 About this book This manual is both a tutorial and a reference manual for the Leica TCS SP. We intend this manual for the novice and the experienced user. If you are a new user, you should start with the chapter "Running the LCS Software,"...
- Page 4 Leica Microsystems Heidelberg GmbH General Documentation Conventions Button The buttons provided on the user interface of the Leica Confocal Software. Buttons are marked with icons and/or have an (often abbreviated) English label. They either trigger actions directly or open dialog windows. Menu The menus are divided into the categories of File, View, Macro, Tools, Window and Help and are displayed in the menu bar located at the upper edge of the user interface.
- Page 5 LEICA. The LEICA TCS SP system allows you to image a single focal plane as well as a series of planes— horizontal or vertical. A single vertical section or xz-scan allows for a side view of the specimen.
- Page 6 2. Thus, the optical resolution along the z-axis amounts to approximately one half of the resolution within the focal plane. The LEICA TCS SP microscope is a no-compromise true point-scanning system with extremely high sensitivity and theoretical maximum x-, y- and z-resolution.
- Page 7 General Integration The Leica TCS SP was designed as an integrated system. Optical and mechanical elements work seamlessly with computer hardware and software. The integrated Leica Confocal Software package supports the complete imaging process, from optical sectioning, through image processing and analysis (which is the main application), to hardcopy output.
- Page 8 No part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or storing in a retrieval system, or translating into any language in any form without the express written permission of Leica Microsystems Heidelberg GmbH.
- Page 9 The software described in this document is furnished under a License Agreement which is included with the product. This agreement specifies the permitted and prohibited uses of the product. Leica TCS SP User Manual English Part no.: 159330193 / Vers.: 31102002...
- Page 10 The owner or operator will be fully liable for all consequences which may result, if the device is opened by persons other than authorized Leica service staff, if it is not serviced or maintained properly or if it is used for purposes other than those described in the accompanying documentation and the online help facility.
- Page 11 3B lasers and high-voltage electrical equipment during installation, operation and maintenance. Operation and maintenance procedure changes are performed entirely at the user's risk. The Leica TCS SP used in accordance with the instructions is harmless. The user is responsible for compliance with current safety regulations. For safety reasons, only trained Leica service personnel is permitted in the laser area.
- Page 12 To reduce the risk of fire hazard and electrical shock, do not expose the unit to rain or humidity. Do not open the cabinet. All maintenance is to be performed by an authorized Leica TCS SP User Manual English Part no.: 159330193 / Vers.: 31102002...
- Page 13 Leica Microsystems Heidelberg GmbH has done everything possible to maximize user safety and minimize health risks applying special safety measures in accordance with several safety directives. The user must operate the TCS SP in accordance with the instructions in order to ensure safety. Several important points are listed below:...
- Page 14 Lasers that are generally harmless if inadvertently viewed directly, including intrabeam viewing; however, these lasers may represent a danger if collector optics are used. Class 3B Leica TCS SP User Manual English Part no.: 159330193 / Vers.: 31102002 Page 14 of 278...
- Page 15 (non-pulsed) < 25 < 5 continuous wave (non-pulsed) HeNe < 3 < 1 continuous wave (non-pulsed) HeNe < 15 < 4 continuous wave (non-pulsed) Leica TCS SP User Manual English Part no.: 159330193 / Vers.: 31102002 Page 15 of 278...
- Page 16 < 15 mW HeNe 633 nm CLASS IIIb LASER PRODUCT LASER RADIATION AVOID DIRECT EXPOSURE TO BEAM < 400 mW ArKr 457-675 nm CLASS IIIb LASER PRODUCT Leica TCS SP User Manual English Part no.: 159330193 / Vers.: 31102002 Page 16 of 278...
- Page 17 Confocal systems operated with a UV laser feature a special microscope optics. For this reason, retrofitting a confocal system designed for the visible spectral range with a Leica TCS SP User Manual English Part no.: 159330193 / Vers.: 31102002 Page 17 of 278...
- Page 18 AVOID EXPOSURE IS EMITTED FROM THIS APERTURE for systems WITH UV laser VISIBLE AND INVISIBLE LASER RADIATION AVOID EXPOSURE IS EMITTED FROM THIS APERTURE Leica TCS SP User Manual English Part no.: 159330193 / Vers.: 31102002 Page 18 of 278...
- Page 19 AVOID EXPOSURE IS EMITTED FROM THIS APERTURE for systems WITH UV laser VISIBLE AND INVISIBLE LASER RADIATION AVOID EXPOSURE IS EMITTED FROM THIS APERTURE Leica TCS SP User Manual English Part no.: 159330193 / Vers.: 31102002 Page 19 of 278...
- Page 20 AVOID EXPOSURE TO BEAM 4. Supply unit (cover) for systems with HeNe laser DANGER LASER RADIATION WHEN OPEN AVOID EXPOSURE TO BEAM for systems with ArKr laser Leica TCS SP User Manual English Part no.: 159330193 / Vers.: 31102002 Page 20 of 278...
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