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Summary of Contents for Stalwart STM-2092
- Page 1 Inverted Biological Microscope Model Number STM-2092 Instruction Manual To ensure correct operation, please read this manual thoroughly before using the microscope and keep it near the product for easy reference.
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Page 2: Table Of Contents
CONTENTS User notice ............................3 1. Application ..........................3 2. Safety Precaution ........................3 3. Maintenance and Storage ...................... 4 4. Safety Symbols ........................5 I. Nomenclature ..........................6 2. Installation ........................... 6 2-1 Installation Diagram ......................6 2-2 Installing steps ........................7 2-2-1 Installing and replacing the lamp（Fig.4）... - Page 3 6-3-1 Selecting the light path ..................26 6-3-2 installing the photography set ................26 7. Specifications ........................... 27 7-1 Main specifications ......................27 7-2 Objective Specifications ....................28 8. Configuration table for inversed microscope STM-2092 ............28 9. Troubleshooting ........................ ...
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Page 4: User Notice
User notice 1. Application Inversed Biological microscope is the dedicated microscope for biological and medical areas, applied in the field of microscopic measurement, health agencies, laboratories, research institutes and universities and other units for biology, genetics, immunology, chemistry, environment protection, oceanography, pharmacology, bacterial observation, education and professional studies. -
Page 5: Maintenance And Storage
any liquid. Otherwise, the instrument will be damaged. 4. When working, the lamp house on the top of the arm③ (Fig.1) will become very hot, be sure there is enough room around the lamp house (especially the top side for cooling). 5. -
Page 6: Safety Symbols
4. Do not disassemble any part of the microscope as this could result in malfunction or reduced performance. 5. If no objectives are mounted, be sure to cover the objective mounting threaded positions on the revolving nosepiece to prevent any dust and spilled culture solution from getting on the lenses inside. -
Page 7: Nomenclature
I. Nomenclature Lamp house Filter holder Illumination column Eyepieces Phase contrast slider Stage Observation tube Condenser Mechanical ruler Auxiliary stage 35/54 mm Petri dish holder Objective Terasaki holder 4x, 10x, 20x, 40x Nosepiece Main switch Main frame Light intensity control knob Fig.2 INVERSED BIOLOGICAL MICROSCOPE 2. -
Page 8: Installing Steps
Keep well with the supplied S1.5 and S2 hexagon wrench. When changing the components, you will need it again. ④ Fig.3 2-2 Installing steps 2-2-1 Installing and replacing the lamp（Fig.4） Please use the specified halogen Lamp 6V30W. 1. Hold to the bulb ①after you wrap it with gauze or other protection materials, then depress the plugs②... -
Page 9: Installing The Lamp House（Fig.5
2. Replacing the lamp when using or soon after When using, or soon after it is turned off, the lamp, the lamp house and nearby parts will be very hot and will cause serious burns. Please turn the main switch to “O” (off), pull up power plug, and make sure the bulb, the lamp house and periphery are all cool. - Page 10 at its lower limit. ★ For ensuring the safety of the instrument during transportation, the nosepiece is located in the lowest position and the tension adjustment collar② is adjusted to an appropriate tension while leaving the factory. 2. Screw the lowest magnification objective onto nosepiece from the nearside, then turn the nosepiece clockwise, mount other objectives according the magnification sequence of low to high.
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Page 11: Installing The Stage Lengthen Splint And The Mechanical Ruler（Fig. 8
2-2-4 Installing the stage lengthen splint and the mechanical ruler（Fig. 8） ◎ Stage lengthen splint can be installed at either side of the stage to enlarge the work surface. can’t install mechanical ruler together at the same side. ◎ Generally, the mechanical ruler will be installed at the right side for comfortable adjustment. -
Page 12: Installing The Color Filters
Fig.10 2-2-7 Installing the color filters （Fig.11） Let the filters cool down sufficiently before INVERSED BIOLOGICAL MICROSCOPE replacing them. Take out the filter holder① and insert the required filters②. Push the filter down to the bottom as shown in Fig.11 ③ so that it does not tilt. If the filter is inclined or is not pushed down to the bottom④, it may fall off the filter holder. -
Page 13: Connecting The Power Cord
（Fig.12,13 &14） 2-2-8 Connecting the power cord ★ Cables and cords are vulnerable when bent or twisted. Never subject them to excessive force. 1.Make sure that the main switch of the power supply is set to “o”(OFF)before connecting cables.(Fig.12) 2.Connect the plug④ of the illumination column firmly to its jack⑤on the rear of the microscope.(Fig.13) 3. -
Page 14: Controls
2-2-9 (Fig.12-14) Replacing the fuse ★ Do remember to turn the main switch① to the state of “O” (off) before replacing the fuse, and unplug the power cord②. Rotate the fuse kits out of the holder by the “--”type screwdriver, replace a new fuse, then rotate back to the holder again. -
Page 15: Using The Controls
4. Using the controls 4-1 Microscope Frame (Fig.16） 4-1-1 Turning on the lamp Connect the power supply, turn on the main switch①(shown in Fig.16)which on the bottom side of the base to “－”(on). 4-1-2 Adjusting the brightness( Fig.16） Turning the brightness adjustment knob② clockwise, the voltage raise, and the brightness strengthen;... -
Page 16: Stage
4-2 stage 4-2-1 Placing the specimen（Fig.18& Fig.19） Put the specimen in the center of the stage. ★ to obtain the best image effect, please select the containers, such as culture dish culture bottle, with bottom thickness 1.2mm, same thickness is also required for the specimen slide. -
Page 17: Moving The Specimen
4-2-2 Moving the specimen Turn the X-axis and Y-axis knobs of the mechanical stage or move the specimen directly by hand. ★ Be careful when changing objectives. When objectives are switched after observing the specimen with an objective with short working distance, the newly selected objective may interfere with the stage center plate or Petri dish holder. -
Page 18: Adjusting The Interpupillary Distance
4-3-2 Adjusting the interpupillary distance (Fig.20-21) While looking through the eyepieces, move both eyepieces until the left and right fields of view coincide completely. ★ The reticle on the interpupillar distance indicator③, pointed by the spot “.” ②on the eyepiece holder, shows the scale of the interpupillar distance.（Fig.20）... -
Page 19: Illumination
Light path Light Intensity ratio Application switching knob Both the upper and 100% for binocular observation Dark specimen observation lower knob is set to gap position 80% for binocular eyepieces，20% for The upper knob is Observation bright set to“PHO”position TV/photography specimens, photography, observation... -
Page 20: Using The Aperture Diaphragm
Filter Application Monochrome contrast filter (green) IF550 Color temperature conversion filter (for observation and photomicrography) Exposure time compensation in photomicrography SIF800 XDINVERSED BIOLOGICAL MICROSCOPE -202 型倒置生物显微 70-80% 30-20% Fig.24 （Fig.24） 4-4-2 Using the aperture diaphragm ◎ the aperture iris diaphragm determines the numerical aperture of the illumination system in bright field observation.Only when the numerical aperture of the objective and the illumination system being matching, you can obtain the higher image resolution and contrast, and the increased depth of focus, too. -
Page 21: Removing The Condenser Lens
(Fig.25) 4-4-3 Removing the condenser lens To provide more working distance, turn the condenser’s lower section ① and remove it. The height of Petri dish can be up to 150mm. ★ When you do this, however, keep in mind that proper illumination cannot be achieved. -
Page 22: Phase Contrast Slider
◎phase contrast objective 10x, 20x, 40 x; ◎installation type: refer to 2-2-4: replace the normal objective with phase contrast objective on nosepiece Fig.26 （Fig.27） 5-1-2 Phase contrast slider ◎ Center adjustable phase contrast slider ● The light ring was centered beforehand, so it needn’t adjust in the using process. If the ring is not in the center, you could adjust it by the centering bolt with S2 wrench. -
Page 23: Centering The Light Annulus
Fig.28 (Fig.29-30) 5-2-2 Centering the light annulus Usually you needn’t the operation of centering. If necessary, please accord to the following steps: 1. Place a specimen on the stage and bring it into focus. 2. Replace the eyepiece in the sleeve with the centering telescope. 3. -
Page 24: Microscope Photography And Video
absolutely sure the light annulus① is not deviating from the phase annulus②. If there is any deviation, perform the centering procedure with the other objectives again. ★ Optimum performance cannot be achieved if the light annulus is not properly centered. ★... -
Page 25: Installing The Video Set
6-1-2 Installing the video set (Fig.32) 1. Take away the dust cap① on trinocular tube. 2. Inset the video accessory② into trinocular tube and screw the pressing ring③ tightly. 3. Screw the mount adapter with CCD camera into the video accessory port② in the direction as shown in Fig.32. -
Page 26: Focus
● Before connecting the camera and the latch notch on the photography accessory, remove the camera lens firstly, then connect the lens port with the accessory. Pay attention to the notch type, please. ● Magnification of micrograph= objective magnification ×camera lens magnification ★... -
Page 27: Digital Photography
6-3 Digital photography 6-3-1 Selecting the light path ★ Just used in the trinocular observation Refer to section 6-1-1 for operation information. More details about light path selection can be obtained in section 4-3-4. Digital Camera Fig.34 6-3-2 installing the photography set (Fig.34) 1. -
Page 28: Specifications
7. Specifications 7-1 Main specifications Optical Infinite Optical System system Seidentopf binocular viewing head Inclined at 45°; Viewing Division ratio: 80% of Binocular Viewing and 20% of Video Viewing & Tube Micrography Eyepiece Wide Field Eyepiece 10X, Linear Field of View : Ф20 mm Nosepiece Quintuple Nosepiece Infinite Long Working Distance Plan Achromatic objective: 4X... -
Page 29: Objective Specifications
Long Working ∞ 1.2mm Distance Plan Phase 0.55 ∞ 1.2mm Contrast Objective 8. Configuration table for inversed microscope STM-2092 ITEM SPECIFICATION QUANTITY Main Body Main frame of inversed microscope STM-2092 4X（plan） Objective 10X（plan phase contrast） （Infinite system） 20X（plan phase contrast）... -
Page 30: Troubleshooting
Digital Optional photography accessory CCD transfer lens Optional 135 camera Optional accessory Fluorescent B,G,U,V Optional attachment Specified Power Cord Spare lamp 6V30W Hexagon wrench S1.5,S2 1.ea. Dust cover User manual Certificate of inspection SED BIOLOGICAL MICROSCOPE 9. Troubleshooting Under certain condition, some no-fault factors will bring a reversible influence to the instrument’s performance. - Page 31 the color filter is stopped evenly Push it in all the way illuminated. halfway The phase contrast slider Move the slider until it is not located in the clicks into place proper position 3. Dirt or dust is Replace with a clean Dirt/dust on the specimen specimen visible in the field...
- Page 32 Moving the culture dish When looking at the edge until you obtain the phase of the culture dish, the contrast effect. You also phase contrast ring and could demount the slider, the light ring is deviated and set the field from each other diaphragm lever to “...
- Page 33 The interpupillar distance Adjust the interpupillar is not correct distance The diopter is not right Adjust the diopter Upon looking into The field of view eyepieces, try looking at of one eye does the overall field before not match that of Your view is not concentrating on the the other...
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