Opentrons Thermocycler GEN2 Instruction Manual
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Opentrons Thermocycler GEN2 Instruction Manual

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Thermocycler GEN2
Instruction Manual
Opentrons Labworks Inc.
Edition
GEN2, January 2023

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Summary of Contents for Opentrons Thermocycler GEN2

  • Page 1 Thermocycler GEN2 Instruction Manual Opentrons Labworks Inc. Edition GEN2, January 2023...

  • Page 2: Table Of Contents

    Table of Contents Product and Manufacturer Description ­ – ­Product­Description ­ – ­Manufacturer­Description Safety Information and Regulatory Compliance ­ – ­Safe­Use­Specifications ­ ­ – ­Safety­Warning­Labels ­ ­ – ­Instrument­Safety­Warnings ­ ­ – ­Standards­Compliance Product Specifications ­ – ­Model­Number ­ – ­Included­Parts ­...

  • Page 3: Product And Manufacturer Description

    Product and Manufacturer Description PRODUCT DESCRIPTION The­Opentrons­Thermocycler­Module­GEN2­provides­on-deck,­fully­ automated­thermocycling.­The­module­enables­automation­of­ upstream­and­downstream­workflow­steps. MANUFACTURER DESCRIPTION Opentrons Labworks Inc 45-18­Ct­Square­W Long­Island­City,­NY­11101...

  • Page 4: Safety Information And Regulatory Compliance

    Safety Information and Regulatory Compliance Opentrons­strongly­recommends­that­you­follow­the­safe­use­ specifications­listed­in­this­section­and­throughout­this­manual. Note: The Thermocycler heats and cools very quickly during operation. SAFE USE SPECIFICATIONS Input and Output Connections The­Thermocycler­has­the­following­power­input­requirements,­which­ are­met­by­the­included­power­supply. Warning:­Do­not­replace­the­power­supply­cable­unless­at­the­ direction­of­Opentrons­Support. Power­Supply­(AC): Voltage:­100–240­V ◾ Frequency: 50/60 Hz ◾ Current:­8.5–5­A ◾ Overvoltage:­Category­II­ ◾ THERMOCYCLER MODULE GEN2...
  • Page 5 Acceptable for Storage and for System System Operation Transportation Operation +20­to­+25­°C +2­to­+40­°C −10­to­+60­°C Ambient Temperature 30–80%,­non- 30–80%,­non- 10–85%,­non- Relative condensing condensing­ condensing­ Humidity (below­30­°C) (below­30­°C) Approximately­ Up­to­2000­m­ Up­to­2000­m­ Altitude 500­m­above­ above­sea­level above­sea­level sea­level Opentrons­has­validated­the­Thermocycler’s­performance­in­the­ conditions­recommended­for­system­operation,­and­operation­in­ those­conditions­should­provide­optimal­results.­The­Thermocycler­is­ safe­to­use­in­conditions­acceptable­for­system­operation,­but­results­ may­vary.­The­device­should­not­be­powered­on­or­used­in­conditions­ outside­of­those­bounds.­The­storage­and­transportation­conditions­ only­apply­when­the­device­is­completely­disconnected­from­power­ and­other­equipment.
  • Page 6 Safety Information and Regulatory Compliance Warning:­Setting­the­Thermocycler­to­hold­a­temperature­below­the­ ambient­temperature­for­a­long­time­can­cause­condensation­to­develop­in­ and­on­the­device.­Do not set the plate temperature below the ambient temperature for more than 2 hours,­as­the­resulting­condensation­could­ affect­performance­or­damage­the­module. If­you­notice­condensation­on­the­Thermocycler’s­plate­after­use,­remove­ any­labware­from­the­module­and­use­the­Opentrons­App­to­set­a­ temperature­of­40­°C­until­the­condensation­is­no­longer­visible. Software Requirements The­Thermocycler­requires­version­6.2.0­or­newer­of­the­Opentrons­App­ and­OT-2­robot­server.­ You­can­download­the­Opentrons­App­for­Windows,­macOS,­or­ Ubuntu­at­https://opentrons.com/ot-app/. Labware Compatibility The­Thermocycler­is­compatible­with­full-skirted,­96-well­PCR­plates.­ Opentrons’­performance­specifications­are­based­on­use­with­Opentrons­ Tough­PCR­Plates,­which­you­can­purchase­at­https://shop.opentrons.com.­ THERMOCYCLER MODULE GEN2...

  • Page 7: Safety Warning Labels

    Safety Information and Regulatory Compliance SAFETY WARNING LABELS Warning­labels­posted­on­the­Opentrons­Thermocycler­and­in­this­ manual­warn­you­about­sources­of­potential­injury­or­harm.­Table­1­ provides­a­key­to­each­safety­warning­label.­ NOTE:­French­safety­warnings­are­listed­in­the­back­of­the­manual.­ (REMARQUE:­Les­avertissements­de­sécurité­en­français­se­trouvent­à­ la­fin­du­manuel.) CAUTION: Risk of danger! This symbol identifies instrument components that pose a risk of personal injury or instrument damage if improperly handled. Wherever this symbol appears, please consult the manual for further information on safe handling before proceeding.

  • Page 8: Instrument Safety Warnings

    Do not attempt to repair or remove the outer case of the Opentrons Thermocycler or its power supply unless directed by Opentrons Support. Failure to do so puts you at risk of electrical shock. Warning about risk of burning. The Opentrons Thermocycler generates enough heat to cause serious burns.

  • Page 9: Standards Compliance

    Safety Information and Regulatory Compliance STANDARDS COMPLIANCE The­Thermocycler­has­been­tested­and­found­to­be­in­compliance­ with­all­applicable­requirements­of­the­following­safety­and­ electromagnetic­standards: Safety IEC/UL/CSA­61010-1­Safety­Requirements­for­Electrical­Equipment­ ◾ for­Measurement,­Control,­and­Laboratory­Use­–­Part­1:­General­ Requirements IEC/UL/CSA­61010-2-010­Particular­Requirements­for­Laboratory­ ◾ Equipment­for­the­Heating­of­Materials Electromagnetic Compatibility EN/BSI­61326-1­Electrical­Equipment­for­Measurement,­Control­ ◾ and­Laboratory­Use­–­EMC­Requirements­–­Part­1:­General­ Requirements EN­55011­Industrial,­Scientific­and­Medical­Equipment­–­Radio- ◾ Frequency­Disturbance­Characteristics­–­Limits­and­Methods­of­ Measurement FCC­47CFR­Part­15­Subpart­B­Class­A:­Unintentional­Radiators ◾ IC­ICES-003­Spectrum­Management­and­Telecommunications­ ◾ –­Interference-Causing­Equipment­Standard­–­Information­ Technology­Equipment­(Including­Digital­Apparatus)
  • Page 10 Safety Information and Regulatory Compliance FCC Warnings and Notes Warning:­Changes­or­modifications­to­this­unit­not­expressly­approved­by­ Opentrons­could­void­the­user’s­authority­to­operate­the­equipment.­ This­device­complies­with­part­15­of­the­FCC­Rules.­Operation­is­subject­ to­the­following­two­conditions:­(1)­This­device­may­not­cause­harmful­ interference,­and­(2)­this­device­must­accept­any­interference­received,­ including­interference­that­may­cause­undesired­operation. Note:­This­equipment­has­been­tested­and­found­to­comply­with­the­ limits­for­a­Class­A­digital­device,­pursuant­to­part­15­of­the­FCC­Rules.­ These­limits­are­designed­to­provide­reasonable­protection­against­ harmful­interference­when­the­equipment­is­operated­in­a­commercial­ environment.­This­equipment­generates,­uses,­and­can­radiate­radio­ frequency­energy­and,­if­not­installed­and­used­in­accordance­with­ the­instruction­manual,­may­cause­harmful­interference­to­radio­ communications.­Operation­of­this­equipment­in­a­residential­area­is­likely­ to­cause­harmful­interference­in­which­case­the­user­will­be­required­to­ correct­the­interference­at­their­own­expense. Canada ISED Compliance Canada­ICES-003(A)­/­NMB-003(A) This­product­meets­the­applicable­Innovation,­Science­and­Economic­ Development­Canada­technical­specifications. Le­présent­produit­est­conforme­aux­spécifications­techniques­applicables­ d’Innovation,­Sciences­et­Développement­économique­Canada. CISPR 11 Class A Caution:­This­equipment­is­not­intended­for­use­in­residential­ environments­and­may­not­provide­adequate­protection­to­radio­reception­ in­such­environments.

  • Page 11: Product Specifications

    Product Specifications MODEL NUMBER TCG2 INCLUDED PARTS (1) Thermocycler (1) Power Supply and Power Supply Connector (1) Power Cable (1) USB Cable (5) Rubber Automation Seals (10) Opentrons Tough PCR Plates...

  • Page 12: Physical Specifications

    Product Specifications PHYSICAL SPECIFICATIONS Dimensions: ◾ Lid­open:­244.95­mm­L­x­172­mm­W­x­310.1­mm­H Lid­closed:­244.95­mm­L­x­172­mm­W­x­170.35­mm­H Weight:­8.4­kg­(including­rear­duct) ◾ TEMPERATURE PROFILE Thermal­block­temperature­range:­4–99­°C ◾ Thermal­block­maximum­heating­ramp­rate:­4.25­°C/s­ ◾ between­ambient­and­95­°C Thermal­block­maximum­cooling­ramp­rate:­2.0­°C/s­ ◾ between­95­°C­and­ambient Lid­temperature­range:­37–110­°C ◾ Lid­temperature­accuracy:­±1­°C ◾ LED STATUS LIGHT The­Thermocycler­has­a­strip­of­LEDs­that­can­display­four­colors:­ white,­orange,­red,­and­blue.­The­possible­module­conditions­ indicated­by­the­light­strip­are: LED COLOR AND BEHAVIOR MODULE CONDITIONS Solid­white Idle Flashing­orange Error Pulsing­red Changing­to­hot­temperature­(>23­°C) Solid­red...

  • Page 13: Setup

    Setup VENTILATION REQUIREMENTS To­allow­for­sufficient­fan­output­from­the­Thermocyler,­at­least­20­ cm­/­8­inches­of­empty­space­is­required­between­the­unit­and­a­ wall. 20 cm FRONT 8”...
  • Page 14 Setup Also­for­ventilation,­and­to­provide­enough­space­for­the­power­and­ USB­cables­to­connect­to­the­module,­Opentrons­advises­using­the­ OT-2­side­and­rear­panels­shown­below: Note: These panels are included with newer models of the OT-2. They only need to be replaced on older models of the OT-2. To­add­new­panels­to­your­OT-2: THERMOCYCLER MODULE GEN2...

  • Page 15: Setup Steps

    Setup SETUP STEPS Deck Placement and Connection Carefully place the Opentrons Thermocycler in deck slots 7, 8, 10, and 11. Lift the module carefully, using two hands (the module weighs 8.4 kg, which is quite heavy for its size).
  • Page 16 Setup Connect the Thermocycler to the OT-2 and power supply by plugging in the USB cable and screwing the power cable into the unit as shown. Be sure to route the cables through the opening in the polycarbonate side panel. Connect the USB cable to the module and an open USB port at the back of the robot Connect the power supply connector to the...
  • Page 17 Setup Once everything is connected, power on the OT-2 and the Thermocycler power supply. If you see a white light on the Thermocycler, it is powered on. You can open and close the lid of the Thermocycler by pressing the round button on the front.
  • Page 18 Setup Rubber Automation Seals Your­Opentrons­Thermocycler­comes­with­5­rubber­automation­ seals­to­help­reduce­evaporation.­Each­seal­must­be­sterilized­ before­use­and­can­be­used­for­several­runs.­You­should­check­ the­state­of­the­seal­after­each­run­and­replace­it­if­needed.­ Worn­or­damaged­seals­may­result­in­increased­evaporation­of­ samples.­ Apply a seal to the lid of the module, not directly to the plate, to enable pipetting access. After applying the seal, wipe it with a 1:10 dilution of bleach and let it dry. Wipe it down with molecular biology grade water and let it dry again.

  • Page 19: Plate Latching

    Setup PLATE LATCHING Thermocycler­GEN2­does­not­include­a­plate­latch,­and­latching­ plates­with­a­hard­polycarbonate­frame­should­not­be­necessary.­ If­your­application­requires­softer­plates­and­you­have­problems­ with­plates­sticking­to­the­seal­when­opening­the­lid,­contact­ Opentrons­Support­to­obtain­a­plate­latch­mechanism. EMERGENCY LID OPENING In­case­of­a­power­outage,­connectivity­issue,­or­other­electrical­ failure,­the­Thermocycler­lid­may­not­open.­If­you­need­to­ manually­open­it­to­retrieve­your­samples:­ Turn the unit off (if possible) and unplug it. Insert the included hex wrench into the circular slot on the left side of the module, as shown.

  • Page 20: Maintenance

    Do­not­clean­the­temperature­indicator­LED­strip,­power­button,­ power­input­port,­or­USB­port­of­the­Thermocycler.­ All­other­surfaces­are­compatible­with: 10%­bleach­solution ◾ 70%­ethanol ◾ 99%­isopropyl­alcohol ◾ Spray­one­of­these­cleaners­onto­a­paper­towel­and­use­it­to­wipe­the­ following­parts­of­the­Thermocycler: PCR­top­plate ◾ Lid­heating­plate ◾ Module­housing/body ◾ Lid­plastic­housing ◾ Sheet­metal­housing ◾ Rubber­seal ◾ When­done,­wipe­down­these­parts­with­a­dry­paper­towel. Avoid­introducing­cleaning­substances­into­the­USB­port­or­power­ port.­Never­douse­or­spray­the­module­directly­with­cleaner. Repair Users­should­not­attempt­to­service­or­repair­the­Thermocycler­ Module­themselves.­If­you­have­concerns­about­your­module’s­ performance­or­require­repairs,­please­contact­Opentrons­Support. Warranty All­hardware­purchased­from­Opentrons­is­covered­under­a­1-year­ standard­warranty.­Opentrons­warrants­to­the­end-user­of­the­ products­that­they­will­be­free­of­manufacturing­defects­due­to­ part­quality­issues­or­poor­workmanship­and­also­warrants­that­ the­products­will­materially­conform­to­Opentrons’­published­ specifications. THERMOCYCLER MODULE GEN2...

  • Page 21: Étiquettes D'avertissement De Sécurité

    Étiquettes d’avertissement de sécurité Les­étiquettes­d’avertissement­affichées­sur­le­Thermocycler­ Opentrons­et­dans­ce­manuel­vous­avertissent­des­sources­ potentielles­de­blessures­ou­de­dommages.­Une­clé­pour­chaque­ étiquette­d’avertissement­de­sécurité­est­référencée­dans­le­tableau­1. ATTENTION: Risque de danger! Ce symbole identifie les composants de l’instrument qui présentent un risque de blessure ou de dommage pour l’instrument s’ils ne sont pas manipulés correctement. Où que ce symbole apparaisse, veuillez consulter le manuel pour plus d’informations sur la sécurité...
  • Page 22 Opentrons ni son alimentation, sauf indication contraire de l’équipe de support technique d’Opentrons. Ne pas le faire vous expose à un risque de choc électrique. Avertissement sur le risque de brûlure.

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