SCIEX 3200 Series System User's Manual
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SCIEX 3200 Series System User's Manual

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SCIEX 3200 Systems
System User Guide
RUO-IDV-05-6475-C
February 2022

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Summary of Contents for SCIEX 3200 Series

  • Page 1 SCIEX 3200 Systems System User Guide RUO-IDV-05-6475-C February 2022...
  • Page 2 SCIEX warranties are limited to those express warranties provided at the time of sale or license of its products and are the sole and exclusive representations, warranties, and obligations of SCIEX. SCIEX...

  • Page 3: Table Of Contents

    Theory of Operation ......................32 3 Ion Source Installation......................34 Prepare for Installation......................34 Install the Probe........................35 Connect the Ion Source Tubing....................36 Install the Ion Source on the Mass Spectrometer..............36 System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 3/114...
  • Page 4 Clean the Curtain Plate...................... 62 Clean the Front of the Orifice Plate..................63 Put the Mass Spectrometer Back in Service..............63 Empty the Source Exhaust Drain Bottle..................63 Inspect the Roughing Pump Oil Level..................66 Storage and Handling......................67 SCIEX 3200 Systems System User Guide 4/114 RUO-IDV-05-6475-C...
  • Page 5 Optimize the APCI Probe Position..................94 Optimize the Nebulizer Current..................96 Optimize the APCI Probe Temperature................96 D Source Parameters and Voltages..................98 TurboIonSpray Probe Parameters..................98 APCI Probe Parameters......................99 Parameter Descriptions......................100 Probe Position........................102 Solvent Composition......................102 System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 5/114...
  • Page 6 Contents E Glossary of Symbols......................104 F Glossary of Warnings......................110 Contact Us..........................113 Customer Training......................... 113 Online Learning Center......................113 SCIEX Support........................113 CyberSecurity........................113 Documentation........................113 SCIEX 3200 Systems System User Guide 6/114 RUO-IDV-05-6475-C...

  • Page 7: Operational Precautions And Limitations

    Refer to the appropriate laboratory reference material and standard operating procedures. Documentation Symbols and Conventions The following symbols and conventions are used throughout the guide. DANGER! Danger signifies an action that leads to severe injury or death. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 7/114...

  • Page 8: Regulatory Compliance

    Canadian ICES-001. Refer to the section: Electromagnetic Interference. • Safety: • CAN/CSA C22.2 No. 61010-1 • CAN/CSA C22.2 No 61010-2-061 Europe • Electromagnetic Compatibility (EMC): Electromagnetic Compatibility Directive 2014/30/EU as implemented in these standards: SCIEX 3200 Systems System User Guide 8/114 RUO-IDV-05-6475-C...

  • Page 9: United States

    • Electromagnetic Compatibility (EMC): • IEC 61326-1 • IEC CISPR 11 (Class A) • IEC 61000-3-2 • IEC 61000-3-3 Refer to the section: Electromagnetic Compatibility. • Safety: • IEC 61010-1 • IEC 61010-2-061 System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 9/114...

  • Page 10: Electrical Precautions

    The covers need not be removed for routine maintenance, inspection, or adjustment. Contact a SCIEX Field Service Employee (FSE) for repairs that require the covers to be removed. • Follow required electrical safe work practices.

  • Page 11: Chemical Precautions

    For the health and safety precautions that must be followed for a chemical, refer to the document: Safety Data Sheet. For storage information, refer to the document: Certificate of Analysis. To find a SCIEX Safety Data Sheet or Certificate of Analysis, go to sciex.com/tech-regulatory.

  • Page 12: System Safe Fluids

    CAUTION: Potential System Damage. Do not use any other fluid until confirmation is received from SCIEX that it does not present a hazard. This is not an exhaustive list. Note: Use only new, freshly prepared LC-MS-grade or better solvents for the LC mobile phases.

  • Page 13: Ventilation Precautions

    WARNING! Puncture Hazard, Ionizing Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Discontinue use of the ion source if the ion source window is cracked or broken, and then contact a SCIEX Field Service Employee (FSE). Any toxic or injurious materials introduced in the equipment will be present in the source exhaust output.

  • Page 14: Physical Precautions

    2 °C per hour, then the resolution and mass calibration might be affected. Electromagnetic Environment Electromagnetic Compatibility Basic Electromagnetic Environment: Environment existing at locations characterized by being supplied directly at low voltage from the public mains network. SCIEX 3200 Systems System User Guide 14/114 RUO-IDV-05-6475-C...
  • Page 15 Operation of this equipment in a residential area is likely to cause harmful interference in which case you will be required to correct the interference, at your own expense. Changes or modifications not expressly approved by the manufacturer could void your authority to operate the equipment. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 15/114...

  • Page 16: Decommissioning And Disposal

    Refer to the section: Storage and Handling. Note: SCIEX will not accept any system returns without a completed Decontamination Form. Contact an FSE to obtain a copy of the form. Do not dispose of system components or subassemblies, including computer parts, as unsorted municipal waste.

  • Page 17: Laboratory Conditions

    The covers need not be removed for routine maintenance, inspection, or adjustment. Contact a SCIEX Field Service Employee (FSE) for repairs that require the covers to be removed. System User Guide...
  • Page 18 Operational Precautions and Limitations WARNING! Personal Injury Hazard. Use SCIEX-recommended parts only. Use of parts not recommended by SCIEX or use of parts for any purpose other than their intended purpose can put the user at risk of harm or negatively impact system performance.

  • Page 19: Principles Of Operation

    (APCI) probe. Refer to the document: Turbo V Ion Source Operator Guide. • SCIEX-supplied computer and monitor with the Analyst software for instrument optimization, acquisition method development, data acquisition, and processing. For computer specifications and requirements, refer to the Software Installation Guide for the Analyst software.

  • Page 20: Hardware Overview

    Integrated Switching Valve Stainless steel, plastic (tubing, fittings, valve body), PEEK (tubing, fittings) Ion Source Stainless steel (tubing, fittings, heat shield, micrometer), bronze (lock nut), rubber, FKM (tubes), silica glass (window), polyester (labels) SCIEX 3200 Systems System User Guide 20/114 RUO-IDV-05-6475-C...
  • Page 21 Roughing Pump Connection Aluminum (hose fitting), Zn-plated steel (hose clamps) I/O Panel Gas Panel Stainless steel (tubing fittings) Ion Source Stainless steel (tubing fittings, heat shield, micrometer), micrometer, silica glass (window), polyester (labels) System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 21/114...
  • Page 22 The Instrument Status lights indicate the status of the mass spectrometer vacuum. When the operational vacuum conditions are satisfied and the mass spectrometer is in analysis mode, the Ready light (green) is illuminated and the Fault light (red) is extinguished. The Fault light flashes SCIEX 3200 Systems System User Guide 22/114...
  • Page 23 Figure 2-4 Sample Introduction Features and Status Indicators Item Description Integrated Switching Valve Load / Inject Instrument Status (Fault / Ready) Integrated Syringe Pump System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 23/114...
  • Page 24 • IEEE-488 (GPIB) port (provides a connection to the acquisition computer) Figure 2-5 I/O Panel Gas Panel The gas panel contains the gas supply connections and the external connections for the source exhaust system. SCIEX 3200 Systems System User Guide 24/114 RUO-IDV-05-6475-C...
  • Page 25 Curtain Gas Supply (Max 60 PSIG) Sheath Gas (Max 105 PSIG) Exhaust Supply (55-60 PSIG) Exhaust Waste Out Valve Waste Out CAD Gas (Max 60 PSIG) Gas 1/Gas 2 (Max 105 PSIG) System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 25/114...

  • Page 26: Ion Source Overview

    The installation procedures can be performed on the following systems: • TripleTOF systems • QTRAP and SCIEX Triple Quad systems In this guide, the software that controls the mass spectrometer is referred to as the control software. The control software varies depending on the mass spectrometer used. Refer to the following table.
  • Page 27 Refer to the section: APCI Mode. Ion Source Components Figure 2-7 Ion Source Components Item Description Primary Materials Sample tubing from a sample supply device Red PEEK Electrode adjustment nut Polyoxymethylene Retaining ring PEEK System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 27/114...
  • Page 28 Samples introduced through the TurboIonSpray probe are ionized within the tubing, by the application of high voltage (IonSpray Voltage). Then they are nebulized by a jet of hot, dry, Zero-grade air from the turbo heaters, creating a mist of small, highly-charged droplets. The SCIEX 3200 Systems System User Guide 28/114...
  • Page 29 A high-velocity jet of nebulizer gas flows around the electrode tip to disperse the sample as a mist of fine particles. It moves through the ceramic vaporization heater to the reaction region of the ion source and then past the corona discharge needle where the sample molecules System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 29/114...
  • Page 30 An ion source sense circuit disables the high-voltage power supply for the mass spectrometer and the source exhaust system under these conditions: • The ion source is not installed or is improperly installed. • A probe is not installed. SCIEX 3200 Systems System User Guide 30/114 RUO-IDV-05-6475-C...
  • Page 31 A vacuum switch installed in the source exhaust sense circuit measures the vacuum in the source. If the vacuum in the source rises above the set point while the probe is installed, then the system enters an exhaust fault, that is, Not Ready, state. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 31/114...

  • Page 32: Theory Of Operation

    Figure 2-10 Ion Optics Path for the 3200 System Item Description Curtain plate DP (orifice) C2B (collar lens) (QTRAP only) DF (deflector) Detector EXB (exit lens) (QTRAP only) ® LINAC collision cell SCIEX 3200 Systems System User Guide 32/114 RUO-IDV-05-6475-C...
  • Page 33 Full spectra data can be acquired from the ions collected in the Q3 region by rapidly scanning the main RF voltage. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 33/114...

  • Page 34: Ion Source Installation

    For optimum stability and performance, the electrode tip should extend between 0.5 mm and 1.0 mm from the end of the probe. Refer to the section: Optimize the TurboIonSpray Probe Position APCI Probe Optimization. SCIEX 3200 Systems System User Guide 34/114 RUO-IDV-05-6475-C...

  • Page 35: Install The Probe

    For the APCI probe only, make sure that the corona discharge needle tip is pointed toward the curtain plate aperture. Refer to the section: Adjust the Position of the Corona Discharge Needle. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 35/114...

  • Page 36: Connect The Ion Source Tubing

    Note: If the probe is not properly installed in the ion source, then the high-voltage power for the mass spectrometer and source exhaust system is turned off. SCIEX 3200 Systems System User Guide 36/114...
  • Page 37 The mass spectrometer recognizes the ion source and then shows the ion source identification in the control software. Connect the red PEEK tubing from the sample supply device to the other side of the grounding union on the ion source. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 37/114...

  • Page 38: Inspect For Leaks

    WARNING! Toxic Chemical Hazard. Wear personal protective equipment, including a laboratory coat, gloves, and safety glasses, to avoid skin or eye exposure. Inspect fittings and tubing to make sure that there are no leaks. SCIEX 3200 Systems System User Guide 38/114...

  • Page 39: Ion Source Optimization

    WARNING! Puncture Hazard, Ionizing Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Discontinue use of the ion source if the ion source window is cracked or broken, and then contact a SCIEX Field Service Employee (FSE). Any toxic or injurious materials introduced in the equipment will be present in the source exhaust output.

  • Page 40: Flow Rate

    Note: To keep the system clean and at optimum performance, adjust the probe position when changing the flow rate. Tip! It is easier to optimize signal and signal-to-noise with flow injection analysis than with on-column injections. SCIEX 3200 Systems System User Guide 40/114 RUO-IDV-05-6475-C...

  • Page 41: Flow Rate And Ion Source Temperature

    Set the ion source temperature to 450. Let the ion source warm up for 30 minutes. The 30-minute warm-up stage prevents solvent vapors from condensing in the cold probe. Start the solvent flow and sample injection. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 41/114...

  • Page 42: Set The Starting Conditions

    Monitor the signal or signal-to-noise of the analytes in the control software. Use the horizontal micrometer to adjust the probe position in small increments to achieve the best signal or signal-to-noise ratio. The probe can optimize slightly to either side of the aperture. SCIEX 3200 Systems System User Guide 42/114 RUO-IDV-05-6475-C...
  • Page 43 The optimal setting for the electrode tip is compound-dependent. The distance that the electrode tip protrudes affects the shape of the spray cone, and the shape of the spray cone affects mass spectrometer sensitivity. Figure 4-1 Electrode Tip Extension Adjustment Item Description Probe Electrode System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 43/114...

  • Page 44: Optimize Source And Gas Parameters And Voltage

    Table 4-1 CUR Parameter Values Mass Spectrometer Starting Value SCIEX 3200 systems Adjust the ion source voltage in increments of 500 V to maximize signal-to-noise. Optimize the Turbo Heater Temperature The optimal heater temperature is dependent on the compound, flow rate, and mobile phase composition.

  • Page 45: Apci Probe Optimization

    Note: When using the APCI probe, make sure that the corona discharge needle is pointing toward the aperture. Optimization Tips Optimization of the ion source minimizes the need for cleaning of the ion source and vacuum interface components. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 45/114...
  • Page 46 • For flow rates greater than 2 mL/min in APCI mode, equilibrate the mass spectrometer before starting the liquid flow, to make sure that the nebulization temperature is reached. SCIEX 3200 Systems System User Guide 46/114...

  • Page 47: Operating Instructions

    WARNING! Personal Injury Hazard. Follow the instructions in the documentation when using the system. The protection provided by the equipment might be impaired if the equipment is used in a manner not specified by SCIEX. Start Up the System WARNING! Electrical Shock Hazard. Make sure that the system can be disconnected from the mains supply outlet in an emergency.

  • Page 48: Syringe Pump

    To stop the syringe pump, click Stop Syringe. Diverter Valve To configure the valve, access the Configuration tab and then make sure that the Use integrated injector/diverter valve check box is selected. SCIEX 3200 Systems System User Guide 48/114 RUO-IDV-05-6475-C...

  • Page 49: Plumb The Diverter Valve In Injector Mode

    When the valve is in Position A, the sample flows through the external loop. When the valve switches to Position B, the sample is injected. Plumb the valve for Injector mode. Figure 5-1 Diverter Valve—Injector Mode Position A System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 49/114...

  • Page 50: Plumb The Diverter Valve In Diverter Mode

    When the valve is in Position A, the sample flow goes to the mass spectrometer. When the valve switches to Position B, the flow goes to waste. Plumb the valve for Diverter mode. SCIEX 3200 Systems System User Guide 50/114...
  • Page 51 Operating Instructions Figure 5-3 Diverter Valve—Diverter Mode Position A Figure 5-4 Diverter Valve—Diverter Mode Position B System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 51/114...

  • Page 52: Shut Down And Vent The System

    Turn off the mass spectrometer convenience switch. Disconnect the mass spectrometer mains supply cable from the mains supply outlet. Disconnect the roughing pump mains supply cable from the mains supply outlet. SCIEX 3200 Systems System User Guide 52/114 RUO-IDV-05-6475-C...

  • Page 53: Operating Instructions - User Workflows

    Configure the LIMS Software User Guide or Help System. Add users to the software and assign roles Laboratory Director Guide or Help System. Archive logs Software User Guide or Help System. Reviewer System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 53/114...
  • Page 54 Operating Instructions — User Workflows Task Reference Review processed results Software User Guide or Help System. Explore data Software User Guide or Help System. Review logs Software User Guide or Help System. SCIEX 3200 Systems System User Guide 54/114 RUO-IDV-05-6475-C...

  • Page 55: Service And Maintenance Information - Mass Spectrometer

    The covers need not be removed for routine maintenance, inspection, or adjustment. Contact a SCIEX Field Service Employee (FSE) for repairs that require the covers to be removed. WARNING! Ionizing Radiation Hazard, Biohazard, or Toxic Chemical Hazard.
  • Page 56 Solutions. To order consumable parts and for basic service and maintenance requirements, contact a QMP or refer to the document: Parts and Equipment Guide. Contact a SCIEX FSE for all other service and maintenance requirements. Table 7-1 Mass Spectrometer Maintenance Tasks...

  • Page 57: Clean The Surfaces

    Clean the external surfaces of the mass spectrometer after a spill or when they become dirty. CAUTION: Potential System Damage. Use only the recommended cleaning methods and materials to avoid damaging the equipment. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 57/114...

  • Page 58: Clean The Front-End

    • Additional peaks that are not part of the sample in full scan or survey scan methods If any of these issues are observed, then clean the mass spectrometer front-end. Required Materials Note: U.S. customers can call 877-740-2129 for ordering information and inquiries. International customers can visit sciex.com/contact-us. SCIEX 3200 Systems System User Guide 58/114...

  • Page 59: Cleaning Best Practices

    WARNING! Toxic Chemical Hazard. Refer to the chemical product safety data sheets and follow all of the recommended safety procedures when handling, storing, and disposing of chemicals. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 59/114...
  • Page 60 • Only dampen the wipe or swab slightly when applying water or cleaning solution. Water, more often than organic solvents, might cause the wipe to deteriorate, leaving residue on the mass spectrometer. SCIEX 3200 Systems System User Guide 60/114 RUO-IDV-05-6475-C...

  • Page 61: Prepare The Mass Spectrometer

    Remove the ion source. Refer to the section: Remove the Ion Source. When the ion source is not in use, store it to protect it from damage and to maintain operating integrity. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 61/114...

  • Page 62: Clean The Curtain Plate

    Using a dampened wipe or small poly swab, clean the aperture. Wait until the curtain plate is dry. Inspect the curtain plate for solvent stains or lint, removing any residue with a clean, slightly damp, lint-free wipe. SCIEX 3200 Systems System User Guide 62/114 RUO-IDV-05-6475-C...

  • Page 63: Clean The Front Of The Orifice Plate

    WARNING! Hot Surface Hazard. Let the Turbo V ion source cool for at least 30 minutes before starting any maintenance procedures. Some surfaces of the ion source and vacuum interface become hot during operation. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 63/114...
  • Page 64 Follow the steps in this procedure to empty the bottle. Remove the ion source. Loosen the clamps connecting the hoses to the cap of the source exhaust drain bottle. SCIEX 3200 Systems System User Guide 64/114 RUO-IDV-05-6475-C...
  • Page 65 Service and Maintenance Information — Mass Spectrometer Figure 7-4 Source Exhaust Drain Bottle Item Description Connection to vent Source exhaust drain tubing: 2.5 cm (1.0 inch) inner diameter (i.d.) Roughing pump exhaust hose: 3.2 cm (1.25 inch) i.d. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 65/114...

  • Page 66: Inspect The Roughing Pump Oil Level

    Inspect the sight glass on the roughing pump to verify that the oil is above the minimum level. If the oil is below the minimum level, then contact the Qualified Maintenance Person (QMP) or SCIEX Field Service Employee (FSE). SCIEX 3200 Systems...

  • Page 67: Storage And Handling

    If the mass spectrometer must be stored for a long time or prepared for shipping, then contact a SCIEX FSE for decommissioning information. To disconnect power from the mass spectrometer, remove the mains supply connector from the AC mains supply.

  • Page 68: Service And Maintenance - Ion Source

    WARNING! Puncture Hazard, Ionizing Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Discontinue use of the ion source if the ion source window is cracked or broken, and then contact a SCIEX Field Service Employee (FSE). Any toxic or injurious materials introduced in the equipment will be present in the source exhaust output.

  • Page 69: Ion Source Handling

    (blue and gray) and surfaces that remain hot for an extended period of time (red). Do not touch the surfaces shown in red while using or removing the ion source. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 69/114...

  • Page 70: Remove The Ion Source

    Wait at least 30 minutes for the ion source to cool. Disconnect the sample tubing from the grounding union. Turn the two source latches up to the 12 o'clock position to release the ion source. SCIEX 3200 Systems System User Guide 70/114...

  • Page 71: Clean The Ion Source Surfaces

    Set the flow rate of the gas for the Curtain Gas interface to the highest setting possible. Wait until the temperature setpoint is reached. Make sure that the probe and sample tubing are flushed thoroughly. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 71/114...

  • Page 72: Remove The Probe

    WARNING! Puncture Hazard. Be careful when handling the electrode. The tip of the electrode is extremely sharp. Prerequisite Procedures • Remove the Ion Source. • Remove the Probe. The probe contains an electrode. Replace the electrode when there is a decrease in performance. SCIEX 3200 Systems System User Guide 72/114 RUO-IDV-05-6475-C...
  • Page 73 Use the 1/4 inch open-ended wrench to remove the retaining nut that holds the electrode tube in the PEEK union. Remove the electrode tube from the retaining nut. Insert the new electrode tube in the retaining nut and then in the PEEK union. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 73/114...

  • Page 74: Replace The Corona Discharge Needle

    If it cannot be removed, then cut off the needle tip to remove it and replace the entire corona discharge needle. Rotate the ion source so that the open side is accessible. SCIEX 3200 Systems System User Guide 74/114 RUO-IDV-05-6475-C...
  • Page 75 Insert the probe and then install the ion source on the mass spectrometer. Refer to the section: Ion Source Installation. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 75/114...

  • Page 76: Replace The Sample Tubing

    The environmental requirements for the storage and transport of the ion source: • Ambient temperature between –30 °C and +60 °C (–22 °F and 140 °F) • Atmospheric pressure between 75 kPa to 101 kPa • Relative humidity not exceeding 99%, non-condensing SCIEX 3200 Systems System User Guide 76/114 RUO-IDV-05-6475-C...

  • Page 77: Mass Spectrometer Troubleshooting

    Mass Spectrometer Troubleshooting This section contains information for troubleshooting basic system issues. Certain activities can only be carried out by a SCIEX-trained Qualified Maintenance Person (QMP) in the laboratory. For advanced troubleshooting, contact a SCIEX Field Service Employee (FSE). Table 9-1 System Issues...
  • Page 78 FSE. The mass spectrometer The mass spectrometer is not Use the Instrument resolution is poor. tuned. Optimization wizard to optimize the mass spectrometer. Refer to the documents: Software User Guide or Help. SCIEX 3200 Systems System User Guide 78/114 RUO-IDV-05-6475-C...
  • Page 79 Corrective Action Sensitivity is Decreased The ion source parameters are not optimized. Optimize the ion source parameters. The mass spectrometer is not optimized. Use the Instrument Optimization wizard to optimize the mass spectrometer. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 79/114...
  • Page 80 Clean the curtain plate. Refer to the section: Clean the Curtain Plate. The orifice plate is dirty. Clean the front of the orifice plate. Refer to the section: Clean the Front of the Orifice Plate. SCIEX 3200 Systems System User Guide 80/114 RUO-IDV-05-6475-C...
  • Page 81 Curtain Gas interface to 45 or 50. Run for a minimum of 2 hours or preferably overnight for best results. For sales, technical assistance, or service, contact an FSE or visit the SCIEX website at sciex.com for contact information. System User Guide...

  • Page 82: A Calibration Ions And Solutions

    Q1 and Q3 3 months to 6 months 3 months to 6 months 3 months to 6 months 3 months to 6 months Table A-2 Suggested Tuning Solutions for the 3200 Series of Instruments System Q1 and Q3 Positive Negative...
  • Page 83 3200 QTRAP LC-MS/MS system 45.00 585.39 933.64 1,223.85 1,572.10 Table A-4 Masses and Polarity for LIT Scans (PPG 3000) Instrument/ Polarity Masses Positive 115.1 500.4 1080.8 1661.2 Negative 121.1 585.4 991.8 1630.1 System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 83/114...

  • Page 84: B Principles Of Operation - Ion Source

    B-1. As a conducting medium, excess charges reside at the surface of the droplet. As the solvent evaporates, the electrical field at the surface of the droplet increases due to the decreasing radius of the droplet. SCIEX 3200 Systems System User Guide 84/114...

  • Page 85: Apci Mode

    The following figure shows the reaction flow of the APCI process for reactant positive ions, the proton hydrates, H System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 85/114...
  • Page 86 Although NO is normally not a major constituent of clean air, the concentration of this species in the ion source is enhanced due to neutral reactions initiated by the corona discharge. SCIEX 3200 Systems System User Guide 86/114 RUO-IDV-05-6475-C...
  • Page 87 As a result, the ionization process yields primarily molecular product ions for mass analysis in the mass spectrometer. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 87/114...

  • Page 88: Apci Ionization Region

    Figure B-4 APCI Ionization Region Item Description Discharge needle tip SCIEX 3200 Systems System User Guide 88/114 RUO-IDV-05-6475-C...
  • Page 89 Operating the APCI probe at temperatures above the optimal temperature might cause thermal decomposition of the sample. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 89/114...

  • Page 90: C Ion Source Optimization

    Note: If the IonSpray Voltage is too high, then a corona discharge can occur. A corona discharge is visible as a blue glow at the tip of the probe. It results in decreased sensitivity and stability of the signal. SCIEX 3200 Systems System User Guide 90/114...

  • Page 91: Set Up The System

    Exceeding the maximum flow rate can cause solvent to accumulate in the ion source. Do not use the ion source if the source exhaust system is not enabled and functioning when the ion source and the probe are properly installed. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 91/114...

  • Page 92: Set Up The System

    If the ion source has been allowed to cool, then do the following. Set the ion source temperature to 450. Let the ion source warm up for 30 minutes. The 30-minute warm-up stage prevents solvent vapors from condensing in the cold probe. SCIEX 3200 Systems System User Guide 92/114 RUO-IDV-05-6475-C...

  • Page 93: Set The Starting Conditions

    Curtain Gas interface, which can produce a noisy signal; prevent contamination of the aperture; and increase the overall signal-to-noise ratio. Table C-2 CUR Parameter Values Mass Spectrometer Starting Value SCIEX 3200 systems System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 93/114...

  • Page 94: Adjust The Position Of The Corona Discharge Needle

    After the probe is optimized, it needs only minor adjustment. If the probe is removed, or if the analyte, flow rate, or solvent composition changes, then repeat the optimization procedure. SCIEX 3200 Systems System User Guide 94/114...
  • Page 95 Use the vertical micrometer to adjust the probe in small increments to achieve the best signal or signal-to-noise ratio. Adjust the black electrode adjustment nut on the probe to move the electrode tube in or out of the probe (to adjust the protrusion). System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 95/114...

  • Page 96: Optimize The Nebulizer Current

    Optimize the APCI Probe Temperature The quantity and type of solvent affects the optimal APCI probe temperature. At higher flow rates, the optimal temperature increases. SCIEX 3200 Systems System User Guide 96/114 RUO-IDV-05-6475-C...
  • Page 97 Ion Source Optimization Adjust the ion source temperature in increments of 50 °C to 100 °C to achieve the best signal or signal-to-noise ratio. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 97/114...

  • Page 98: D Source Parameters And Voltages

    0 V Optimum temperature values depend on the compound and mobile phase composition. Higher aqueous content requires a higher temperature. Zero (0) means no temperature is applied. DP values depend on the compound. SCIEX 3200 Systems System User Guide 98/114 RUO-IDV-05-6475-C...

  • Page 99: Apci Probe Parameters

    Positive: 60 V Positive: 0 V to 300 V Negative: –60 V Negative: –300 V to 0 V Probe vertical micrometer Scale 0 to 13 setting Temperature values depend on the compound. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 99/114...

  • Page 100: Parameter Descriptions

    Controls the heat applied to the sample to vaporize it. The optimal ion temperature source temperature is the lowest temperature at which the sample is vaporized completely. Optimize in increments of 50 °C. SCIEX 3200 Systems System User Guide 100/114 RUO-IDV-05-6475-C...
  • Page 101 If the ion source temperature is set too low, then vaporization is incomplete, and large, and visible droplets are expelled into the ion source housing. If the ion source temperature is set too high, then thermal degradation of the sample occurs. System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 101/114...

  • Page 102: Probe Position

    In the Analyst software, this is the IonSpray Voltage field. Interface heater This parameter is always on for 3200 series systems. Turns the interface heater on and off. Heating the interface helps maximize the ion signal and prevents contamination of the ion optics. Unless the compound the user is analyzing is extremely fragile, we recommend that the user heat the interface.
  • Page 103 They might also suppress the strength of the target compound ion signal. • Triethyl amine (TEA) • Sodium phosphate • Trifluoroacetic acid (TFA) • Sodium dodecyl sulfate System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 103/114...

  • Page 104: E Glossary Of Symbols

    Australian Communications Media Authority (ACMA) EMC Requirements. Alternating current Amperes (current) Asphyxiation Hazard Authorized representative in the European community Biohazard CE Marking of Conformity cCSAus mark. Indicates electrical safety certification for Canada and USA. Catalog number SCIEX 3200 Systems System User Guide 104/114 RUO-IDV-05-6475-C...
  • Page 105 Symbol Description Caution. Consult the instructions for information about a possible hazard. Note: In SCIEX documentation, this symbol identifies a personal injury hazard. China RoHS Caution Label. The electronic information product contains certain toxic or hazardous substances. The center number is the Environmentally Friendly Use Period (EFUP) date, and indicates the number of calendar years the product can be in operation.
  • Page 106 Hertz International safety symbol "Caution, risk of electric shock" (ISO 3864), also known as High Voltage symbol If the main cover must be removed, then contact a SCIEX representative to prevent electric shock. Hot Surface Hazard In Vitro Diagnostic Device...
  • Page 107 Do not expose to rain. Relative humidity must not exceed 99%. Keep upright. Lacerate/Sever Hazard Laser Radiation Hazard Lifting Hazard Magnetic Hazard Manufacturer Moving Parts Hazard Pacemaker Hazard. No access to people with pacemakers. Pinching Hazard System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 107/114...
  • Page 108 Transport and store the system within the specified minimum (min) and maximum (max) levels of relative humidity, noncondensing. Transport and store the system within –30 °C to +45 °C. Transport and store the system within –30 °C to +60 °C. SCIEX 3200 Systems System User Guide 108/114 RUO-IDV-05-6475-C...
  • Page 109 USB 3.0 connection Ultraviolet Radiation Hazard United Kingdom Conformity Assessment Mark Volt Ampere (power) Volts (voltage) WEEE. Do not dispose of equipment as unsorted municipal waste. Environmental Hazard Watts yyyy-mm-dd Date of manufacture System User Guide SCIEX 3200 Systems RUO-IDV-05-6475-C 109/114...

  • Page 110: F Glossary Of Warnings

    Note: If the indicator is tripped, then this container has been dropped or otherwise mishandled. Make a note on the Bill of Lading and then check for damage. Any claims for shock damage require a notation. SCIEX 3200 Systems System User Guide 110/114 RUO-IDV-05-6475-C...
  • Page 111 OR “TILT INDICATOR” ON THE WAYBILL BEFORE ACCEPTING SHIPMENT WAYBILL BEFORE ACCEPTING SHIPMENT AND NOTIFY YOUR LOCAL AB AND NOTIFY YOUR LOCAL AB SCIEX CUSTOMER SUPPORT ENGINEER SCIEX CUSTOMER SUPPORT ENGINEER IMMEDIATELY. IMMEDIATELY. DO NOT UNCRATE. CONTACT YOUR DO NOT UNCRATE. CONTACT YOUR...
  • Page 112 Glossary of Warnings Label Translation (if applicable) WARNING: NO USER SERVICEABLE PARTS WARNING: NO USER SERVICEABLE PARTS INSIDE. REFER SERVICING TO QUALIFIED INSIDE. REFER SERVICING TO QUALIFIED PERSONNEL. PERSONNEL. Note: Consult instructions for use. SCIEX 3200 Systems System User Guide 112/114 RUO-IDV-05-6475-C...

  • Page 113: Contact Us

    SCIEX Now Learning Hub SCIEX Support SCIEX and its representatives maintain a staff of fully-trained service and technical specialists located throughout the world. They can answer questions about the system or any technical issues that might arise. For more information, visit the SCIEX website at sciex.com...
  • Page 114 To find hardware product documentation, refer to the Customer Reference DVD that comes with the system or component. The latest versions of the documentation are available on the SCIEX website, at sciex.com/ customer-documents. Note: To request a free, printed version of this document, contact sciex.com/contact-us.

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